KR880010124A - Method for preparing a highly active promoter gene isolated from DNA of (YA-14) in alkaline resistant Bacillus - Google Patents

Method for preparing a highly active promoter gene isolated from DNA of (YA-14) in alkaline resistant Bacillus Download PDF

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KR880010124A
KR880010124A KR870001038A KR870001038A KR880010124A KR 880010124 A KR880010124 A KR 880010124A KR 870001038 A KR870001038 A KR 870001038A KR 870001038 A KR870001038 A KR 870001038A KR 880010124 A KR880010124 A KR 880010124A
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dna
preparing
highly active
promoter gene
active promoter
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KR870001038A
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Korean (ko)
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KR890003679B1 (en
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구본탁
김진만
공인수
백운화
유주현
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유주현
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

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Abstract

내용 없음No content

Description

알카리 내성 바씰러스속 (YA-14)의 DNA로 부터 분리된 고활성 프로모터 유전자 제조방법Method for preparing a highly active promoter gene isolated from DNA of alkaline resistant Bacillus (YA-14)

제1도는 p-12를 함유하고 있는 바씰러스 서브틸리스 207-25로부터 분리한 플라스미드 DNA와 이를 제한 효소를 절단한후 행한 아가로스 겔 전기 영동의 사진 도해이다.1 is a photographic illustration of plasmid DNA isolated from Bacillus subtilis 207-25 containing p-12 and agarose gel electrophoresis after cleavage of the restriction enzyme.

제2도는 p-12 내의 삽입 유전자로부터 재조합 플라스미드 p-12S와 p-12B1을 제조하는 과정을 나타내는 도면이다.2 is a diagram illustrating a process for preparing recombinant plasmids p-12S and p-12B1 from an insertion gene in p-12.

제3도는 p-12S와 p-12B1을 함유하는 바씰러스 서브틸리스 207-25로부터 분리한 플라스미드 DNA와 이들을 제한 효소로 처리한후 행한 아가로스 겔 전기 영동 사진 도해이다.3 is a diagram of plasmid DNA isolated from Bacillus subtilis 207-25 containing p-12S and p-12B1 and agarose gel electrophoresis after treatment with restriction enzymes.

Claims (1)

토양에서 분리한 알칼리 내성 바씰러스 스페시스 YA-14(KFCC-10308)의 DNA로부터 클로닝한 0.8kb의 프로모터 유전자 단편(BamHI-Sall 유전자 단편)을 함유한 플라스미드 DNA의 제조방법.A method for producing plasmid DNA containing 0.8 kb of promoter gene fragment (BamHI-Sall gene fragment) cloned from DNA of alkaline resistant Bacillus spesis YA-14 (KFCC-10308) isolated from soil. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019870001038A 1987-02-09 1987-02-09 Process for preparing of dna promoter from bacillus sp KR890003679B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019870001038A KR890003679B1 (en) 1987-02-09 1987-02-09 Process for preparing of dna promoter from bacillus sp

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019870001038A KR890003679B1 (en) 1987-02-09 1987-02-09 Process for preparing of dna promoter from bacillus sp

Publications (2)

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KR880010124A true KR880010124A (en) 1988-10-07
KR890003679B1 KR890003679B1 (en) 1989-09-30

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KR1019870001038A KR890003679B1 (en) 1987-02-09 1987-02-09 Process for preparing of dna promoter from bacillus sp

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KR890003679B1 (en) 1989-09-30

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