KR920012431A - Expression vector of human epidermal growth factor - Google Patents

Expression vector of human epidermal growth factor Download PDF

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Publication number
KR920012431A
KR920012431A KR1019900022194A KR900022194A KR920012431A KR 920012431 A KR920012431 A KR 920012431A KR 1019900022194 A KR1019900022194 A KR 1019900022194A KR 900022194 A KR900022194 A KR 900022194A KR 920012431 A KR920012431 A KR 920012431A
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South Korea
Prior art keywords
growth factor
epidermal growth
human epidermal
expression vector
nucleic acid
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KR1019900022194A
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Korean (ko)
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KR920008372B1 (en
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조중명
양재영
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최근선
주식회사 럭 키
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음No content

Description

인간 표피 성장인자의 발현벡터Expression vector of human epidermal growth factor

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 인간 표피 성장인자의 염기 서열 및 아미노산 서열을 나타낸 것이도, 제2도는 본 발명에 따른 효모 발현벡터로의 클로닝 과정을 도시한 것이며, 제3도는 본 발명의 표피 성장인자의 발현벡터를 함유하는 효모의 농축 배양액을 SDS-플라아크릴 아마이드 겔을 사용하여 전기영동한 결과를 나타낸 것이다.Figure 1 shows the nucleotide sequence and amino acid sequence of the human epidermal growth factor, Figure 2 shows the cloning process to the yeast expression vector according to the present invention, Figure 3 shows the expression vector of the epidermal growth factor of the present invention The result of electrophoresis of the concentrated culture solution containing the yeast using SDS-plastic acrylamide gel is shown.

Claims (2)

인간 인슐린 유사 성장인자-Ⅰ의 발현벡터를 제한효소 Pat Ⅰ과 SalⅠ, Xba Ⅰ과 PstⅠ으로 절단하여 각각 글루코알데히드-3-포스페이트 데히드로게나제의 종료유전자를 함유하는 핵산절편과 알콜 데히드로게나제/글루코알데히드-3-포스페이트 데히드로게나제 혼성 작동유전자와 알파 인자의 리더시퀸스를 함유하는 핵산절편, 인간표피 성장인자 유전자의 발현 벡터를 제한효소 Xba Ⅰ과 SalⅠ 으로 절단하여 알파 인자의 카복시말단의 염기쌍이 함유되어 있는 인간표피 성장인자 핵산절편 등을 접합 시켜서 된, ADH-2/GAPDH 혼성 작동유전자와 알파 인자 리더시퀸스, 인간 표피 성장인자 유전자 및 GAPDH 종료유전자로 구성됨을 특징으로 하는 인간 표피 성장인자의 발현벡터.Expression vectors of human insulin-like growth factor-I were digested with restriction enzymes Pat I and Sal I, Xba I and Pst I, and nucleic acid fragments containing the tertiary gene of glucoaldehyde-3-phosphate dehydrogenase and alcohol dehydrogenase, respectively. Nucleic acid fragments containing the glucoaldehyde-3-phosphate dehydrogenase hybrid agonist and alpha factor leader sequence, and the expression vector of the human epidermal growth factor gene were cleaved with restriction enzymes Xba I and Sal I to the carboxy terminus of the alpha factor. Human epidermal growth factor consisting of ADH-2 / GAPDH hybrid agonist, alpha factor leader sequence, human epidermal growth factor gene and GAPDH terminator Expression vector. 제1항에 있어서, 상기 인간 표피 성장인자의 핵산절편은 177개의 염기쌍으로 구성됨을 특징으로 하는 인간표피 성장 인자의 발현벡터.The expression vector of human epidermal growth factor according to claim 1, wherein the nucleic acid fragment of the human epidermal growth factor consists of 177 base pairs. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900022194A 1990-12-28 1990-12-28 Expression vector for epidermal growth factor KR920008372B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019900022194A KR920008372B1 (en) 1990-12-28 1990-12-28 Expression vector for epidermal growth factor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019900022194A KR920008372B1 (en) 1990-12-28 1990-12-28 Expression vector for epidermal growth factor

Publications (2)

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KR920012431A true KR920012431A (en) 1992-07-27
KR920008372B1 KR920008372B1 (en) 1992-09-26

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KR1019900022194A KR920008372B1 (en) 1990-12-28 1990-12-28 Expression vector for epidermal growth factor

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KR920008372B1 (en) 1992-09-26

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