KR950005990A - Expression of human interleukin-4 in yeast - Google Patents
Expression of human interleukin-4 in yeast Download PDFInfo
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- KR950005990A KR950005990A KR1019930015350A KR930015350A KR950005990A KR 950005990 A KR950005990 A KR 950005990A KR 1019930015350 A KR1019930015350 A KR 1019930015350A KR 930015350 A KR930015350 A KR 930015350A KR 950005990 A KR950005990 A KR 950005990A
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- expression vector
- human interleukin
- yeast
- gene
- interleukin
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5406—IL-4
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
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- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
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- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
본 발명은 유전공학적인 방법에 의해 제조된 인간 인터류킨-4유전자, 그를 포함하는 발현 벡터, 상기 발현벡터로 형질전환된 미생물 및 상기 미생물을 이용한 인터류킨-4의 생산 방법에 관한 것이다. 보다 상세하게는, IL-4 단백질의 아미노산 서열을 그대로 유지하면서 유전자의 대량 발현을 가능케 하는 코돈을 사용하여 설계된 인간 인터류킨-4유전자, 상기 유전자를 유비퀴틴 유전자와 결합된 상태로 포함하여 그의 발현시 인간 인터류킨-4와 유비퀴틴의 융합 단백질을 생산하는 발현 벡터, 상기 발현 벡터로 형질 전화된 미생물 및 상기 미생물을 배양함으로써 인간 인터류킨-4를 대량으로 생산하는 방법에 관한 것이다.The present invention relates to a human interleukin-4 gene produced by a genetic engineering method, an expression vector comprising the same, a microorganism transformed with the expression vector, and a method for producing interleukin-4 using the microorganism. More specifically, the human interleukin-4 gene, which is designed using codons capable of mass expression of genes while maintaining the amino acid sequence of the IL-4 protein, comprising the gene in combination with the ubiquitin gene, and human An expression vector for producing a fusion protein of interleukin-4 and ubiquitin, a microorganism transformed with the expression vector, and a method for producing a large amount of human interleukin-4 by culturing the microorganism.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
제1도는 5'-말단에 Sacll 제한 효소 부위, 3'-말단에 Sall 제한 효소 부위를 갖는 IL-4유전자의 염기 서열및 아미노산 서열을 나타낸 것이고,1 shows the nucleotide sequence and amino acid sequence of the IL-4 gene having a Sacll restriction enzyme site at the 5'-end and a Sall restriction enzyme site at the 3'-end,
제2도는 전체 IL-4유전자의 합성올리고머 1,2,3,4,5,6,7,8,9 및 10의 위치 및 그연결 순서를 나타낸 것이고,Figure 2 shows the positions of the synthetic oligomers 1,2,3,4,5,6,7,8,9 and 10 of the whole IL-4 gene and their sequence of connection,
제3도는 IL-4 유전자의 M13 백터로의 클로닝 및 효모발현 벡터로의 클로닝을 도식화한 것이고,3 shows the cloning of IL-4 gene into M13 vector and cloning into yeast expression vector,
제4도는 본 발명의 IL-4 발현 벡터(pYLBC-A/G-UB-IL-4)를 함유하는 효모의 농축 배양액을 SDS 폴리아크릴아미드 젤 전기 영동한 결과를 나타낸 것이다.Figure 4 shows the results of SDS polyacrylamide gel electrophoresis of the concentrated culture of yeast containing the IL-4 expression vector (pYLBC-A / G-UB-IL-4) of the present invention.
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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KR1019930015350A KR100274183B1 (en) | 1993-08-07 | 1993-08-07 | Expression of human interleukin-4 in yeast |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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KR1019930015350A KR100274183B1 (en) | 1993-08-07 | 1993-08-07 | Expression of human interleukin-4 in yeast |
Publications (2)
Publication Number | Publication Date |
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KR950005990A true KR950005990A (en) | 1995-03-20 |
KR100274183B1 KR100274183B1 (en) | 2000-12-15 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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KR1019930015350A KR100274183B1 (en) | 1993-08-07 | 1993-08-07 | Expression of human interleukin-4 in yeast |
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KR (1) | KR100274183B1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100443570B1 (en) * | 2001-07-31 | 2004-08-09 | 크레아젠 주식회사 | The preparation method of recombinant human Interleukin-4 |
-
1993
- 1993-08-07 KR KR1019930015350A patent/KR100274183B1/en not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100443570B1 (en) * | 2001-07-31 | 2004-08-09 | 크레아젠 주식회사 | The preparation method of recombinant human Interleukin-4 |
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Publication number | Publication date |
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KR100274183B1 (en) | 2000-12-15 |
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