KR960031477A - Modified human basic fibroblast growth factor and production method thereof - Google Patents

Modified human basic fibroblast growth factor and production method thereof Download PDF

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KR960031477A
KR960031477A KR1019950002643A KR19950002643A KR960031477A KR 960031477 A KR960031477 A KR 960031477A KR 1019950002643 A KR1019950002643 A KR 1019950002643A KR 19950002643 A KR19950002643 A KR 19950002643A KR 960031477 A KR960031477 A KR 960031477A
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South Korea
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growth factor
fibroblast growth
basic fibroblast
human basic
modified human
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KR1019950002643A
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KR100374310B1 (en
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양재영
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성재갑
주식회사 Lg 화학
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/102—Plasmid DNA for yeast

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
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  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 변형된 인간 염기성 섬유아세포 성장인자, 이를 코드하는 유전자, 상기 유전자와 유비퀴틴의 결합 유전자를 포함하는 발현벡터, 상기 발현, 벡터로 형질전환된 미생물 및 상기 미생물을 배양하여 변형된 인간 염기성 섬유아세포 성장인자를 생산하는 방법에 관한 것으로서, 본 발명에 의해 생산된 변형된 인간 염기성 섬유아세포 성장인자는 기존의 인간 염기성 섬유아세포 성장인자의 35번째 및 60번째 아미노산이 각각 아르기닌과 발린으로 치환된 것으로서 기준의 인간 염기성 섬유아세포 성장인자보다 높은 활성을 나타낸다.The present invention provides a modified human basic fibroblast growth factor, a gene encoding the same, an expression vector containing the gene and the binding gene of ubiquitin, the expression, the microorganism transformed with the vector and the human basic fiber modified by culturing the microorganism. A method for producing blast growth factor, wherein the modified human basic fibroblast growth factor produced by the present invention is substituted with arginine and valine, respectively, of the 35th and 60th amino acids of the existing human basic fibroblast growth factor. It exhibits higher activity than standard human basic fibroblast growth factor.

Description

변형된 인간 염기성 섬유아세포 성장인자 및 이의 생산 방법Modified human basic fibroblast growth factor and production method thereof

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (10)

인간 염기성 섬유아세포 성장인자에서, 35번째 아미노산과 60번째 아미노산이 치환된 변형된 인간 염기성 섬유아세포 성장인자.In a human basic fibroblast growth factor, a modified human basic fibroblast growth factor substituted for the 35th and 60th amino acids. 35번째 아미노산이 아르기닌으로, 60번째 아미노산이 발린으로 치환된 변형된 인간 염기성 섬유아세포 성장인자.A modified human basic fibroblast growth factor wherein the 35th amino acid is substituted with arginine and the 60th amino acid is substituted with valine. 제2항에 있어서, 하기의 아미노산 서열을 갖는 인간 염기성 섬유아세포 성장인자:The human basic fibroblast growth factor of claim 2 having the following amino acid sequence: M A A G S I T T L P A L P E D G G S G AM A A G S I T T L P A L P E D G G S G A F P P G H F K D P K R L Y C R N G G F FF P P G H F K D P K R L Y C R N G G F F L R I H P D G R V D F V R E K S D P H VL R I H P D G R V D F V R E K S D P H V K L Q L Q A E E R G V V S I K G V C A NK L Q L Q A E E R G V V S I K G V C A N R Y L A M K E D G R L L A S K C V T D ER Y L A M K E D G R L L A S K C V T D E C F F F E R L E S N N Y N T Y R S R K YC F F F E R L E S N N Y N T Y R S R K Y T S W Y V A L K R T G Q Y K L G S K T GT S W Y V A L K R T G Q Y K L G S K T G P G Q K A I L F L P M S A K SP G Q K A I L F L P M S A K S (상기에서, 각 아미노산은 표준 단일 약자로 나타냄)(In the above, each amino acid is represented by a standard single abbreviation) 제1항 내지 제3항 중 어느 한 항의 변형된 인간 염기성 섬유아세포 성장인자를 코드하는 DNA.DNA encoding the modified human basic fibroblast growth factor of any one of claims 1 to 3. 제4항에 있어서, 하기의 염기 서열을 갖는 DNA:The DNA of claim 4 having the following nucleotide sequence: 제4항 또는 제5항의 DNA를 포함하는 발현벡터.An expression vector comprising the DNA of claim 4 or 5. 제6항에 있어서, 플라스미드 pYLBC-A/G-UB-bFGF(기탁번호: KCTK 0148BP).The plasmid pYLBC-A / G-UB-bFGF (Accession Number: KCTK 0148BP) according to claim 6. 제6항 또는 제7항의 발현벡터로 형질전환된 미생물.A microorganism transformed with the expression vector of claim 6. 제8항에 있어서, 플라스미드 pYLBC-A/G-UB-bFGF로 형질전환된 효모 사카로마이세스 세레비지애(Saccharomyces cerevisiae) DC04기탁번호(KCTK 0148BP).9. The yeast Saccharomyces cerevisiae DC04 Accession No. (KCTK 0148BP) transformed with plasmid pYLBC-A / G-UB-bFGF. 제8항에 또는 제9항의 미생물을 제조합 단백질의 생산을 위한 통상의 배지에서 통상적인 조건하에 배양하는 것을 포함하는, 변형된 인간 염기성 섬유아세포 성장인자의 생산 방법.10. A method of producing a modified human basic fibroblast growth factor comprising culturing the microorganism of claim 8 or 9 under conventional conditions in a conventional medium for producing a synthetic protein. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임※ Note: The disclosure is based on the initial application.
KR1019950002643A 1995-02-14 1995-02-14 Modified human basic fibroblast growth factor and process for the production thereof KR100374310B1 (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113201554A (en) * 2021-04-01 2021-08-03 芜湖英特菲尔生物制品产业研究院有限公司 Saccharomyces cerevisiae expression long-acting recombinant human bFGF-HSA fusion protein and preparation method of standard substance thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100982178B1 (en) 2008-01-29 2010-09-14 (주)케어젠 Mono?Pegylated Basic Fibroblast Growth Factor Variants and Uses Thereof

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* Cited by examiner, † Cited by third party
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US4956455A (en) * 1984-03-05 1990-09-11 The Salk Institute For Biological Studies Bovine fibroblast growth factor
JPH02504468A (en) * 1987-11-24 1990-12-20 アムジエン・インコーポレーテツド Fibroblast growth factor analog
US5352589A (en) * 1988-09-16 1994-10-04 Farmitalia Carlo Erba S.R.L. Deletion mutant of basic fibroblast growth factor and production thereof
CA2070989A1 (en) * 1989-12-19 1991-06-20 Kazuaki Kitano Production of human basic fgf mutein
ATE138687T1 (en) * 1990-11-23 1996-06-15 American Cyanamid Co CHIMERIC FIBROBLAST GROWTH FACTOR
JP3303211B2 (en) * 1991-04-26 2002-07-15 武田薬品工業株式会社 bFGF mutein and method for producing the same

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113201554A (en) * 2021-04-01 2021-08-03 芜湖英特菲尔生物制品产业研究院有限公司 Saccharomyces cerevisiae expression long-acting recombinant human bFGF-HSA fusion protein and preparation method of standard substance thereof

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