KR960001120A - Expression Vector of Human Pherotropin Gene and Method for Preparing Human Pherotropin Protein in Yeast - Google Patents

Expression Vector of Human Pherotropin Gene and Method for Preparing Human Pherotropin Protein in Yeast Download PDF

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Publication number
KR960001120A
KR960001120A KR1019940012337A KR19940012337A KR960001120A KR 960001120 A KR960001120 A KR 960001120A KR 1019940012337 A KR1019940012337 A KR 1019940012337A KR 19940012337 A KR19940012337 A KR 19940012337A KR 960001120 A KR960001120 A KR 960001120A
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South Korea
Prior art keywords
human
pherotropin
yeast
expression vector
gene
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KR1019940012337A
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Korean (ko)
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KR0135390B1 (en
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조중명
추승호
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성재갑
주식회사 엘지화학
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 유비퀴틴 유전자에 융합된 인간 플레이오트로핀 유전자를 포함하는 효모 발현벡터, 상기 발현 백터에 의해 형질전환된 효모세포 및 상기 효모세포를 이용한 인간 플레이오트로핀 단백질의 제조방법에 관한 것이다.The present invention relates to a yeast expression vector comprising a human fluorotrophin gene fused to a ubiquitin gene, a yeast cell transformed by the expression vector and a method for producing a human fluorotrophin protein using the yeast cell.

Description

인간 플레이오트로핀 유전자의 발현벡터 및 효모에서의 인간 플레이오트로핀 단백질의 제조방법Expression Vector of Human Pherotropin Gene and Method for Preparing Human Pherotropin Protein in Yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 인간 플레이오트로핀 유전자의 염기서열 및 그에 코딩된 아미노산 서열을 나타낸 것이다.Figure 1 shows the nucleotide sequence of the human fluorotrophin gene and the amino acid sequence encoded therein.

제2도는 인간 플레인오트로핀 유전자의 효모 발현벡터로의 클로닝 과정을 나타낸 것이다.2 shows the cloning of the human plain otropin gene into the yeast expression vector.

제3도는 효모에서 발현된 인간 플레이오트로핀을 폴리아크릴 아마이드 젤 전기영동한 결과를 나타낸 것이다.Figure 3 shows the results of polyacrylamide gel electrophoresis of human fluorotropin expressed in yeast.

Claims (5)

유비퀴틴 유전자에 연결된 인간 플레이오트로핀 유전자를 포함하는 효모 발현벡터.Yeast expression vector comprising a human playotropin gene linked to the ubiquitin gene. 제1항에 있어서, pYLBC-A/G-UB-HPTN인 발현벡터.The expression vector according to claim 1, which is pYLBC-A / G-UB-HPTN. 제1항 또는 제2항의 발현 벡터에 의해 형질전환된 효모.Yeast transformed with the expression vector of claim 1. 제3항에 있어서, pYLBC-A/G-UB-HPTN으로 형질전환된 효모 사카로마이세스 세레비지애 DC 04(ATCC 74267).4. The yeast Saccharomyces cerevisiae DC 04 (ATCC 74267) according to claim 3, transformed with pYLBC-A / G-UB-HPTN. 제3항 또는 제4항의 효모를 배양함을 포함하는, 인간 플레이오트로핀 단백질의 제조방법.Method for producing a human fluorotrophin protein, comprising culturing the yeast of claim 3 or 4. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940012337A 1994-06-02 1994-06-02 Victors for expressing human pleiotrophin gene in yeast and KR0135390B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940012337A KR0135390B1 (en) 1994-06-02 1994-06-02 Victors for expressing human pleiotrophin gene in yeast and

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940012337A KR0135390B1 (en) 1994-06-02 1994-06-02 Victors for expressing human pleiotrophin gene in yeast and

Publications (2)

Publication Number Publication Date
KR960001120A true KR960001120A (en) 1996-01-25
KR0135390B1 KR0135390B1 (en) 1998-04-23

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940012337A KR0135390B1 (en) 1994-06-02 1994-06-02 Victors for expressing human pleiotrophin gene in yeast and

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KR (1) KR0135390B1 (en)

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Publication number Publication date
KR0135390B1 (en) 1998-04-23

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