KR950023720A - Expression Vectors of the Human Midkine Gene and Its Expression in Yeast - Google Patents

Expression Vectors of the Human Midkine Gene and Its Expression in Yeast Download PDF

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Publication number
KR950023720A
KR950023720A KR1019940001179A KR19940001179A KR950023720A KR 950023720 A KR950023720 A KR 950023720A KR 1019940001179 A KR1019940001179 A KR 1019940001179A KR 19940001179 A KR19940001179 A KR 19940001179A KR 950023720 A KR950023720 A KR 950023720A
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South Korea
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yeast
expression
gene
human midkine
expression vector
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KR1019940001179A
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Korean (ko)
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KR100260633B1 (en
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조중명
김천형
김승업
무라마쯔 다까시
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성재갑
주식회사 엘지화학
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 유비퀴틴 유전자에 인간 미드카인 유전자가 융합된 재조합 유전자를 포함하는 발현 벡터, 상기 발현 벡터로 형질전환된 효모 및 이를 배양함을 포함하는 인간 미드카인 재조합 단백질의 대량 생산 방법을 제공한다.The present invention provides an expression vector comprising a recombinant gene in which a human midkine gene is fused to a ubiquitin gene, a yeast transformed with the expression vector, and a mass production method of a human midkine recombinant protein comprising culturing the same.

Description

인간 미드카인 유전자의 발현 벡터 및 효모에서의 그의 발현Expression Vectors of the Human Midkine Gene and Its Expression in Yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 인간 미드카인의 아미노산 서열을 나타낸 것이고,1 shows the amino acid sequence of human midkine,

제2도는 인간 미드카인 유전자의 연결 전략 및 효모 발현 벡터의 제조 과정을 나타낸 것이고,2 shows the connection strategy of the human midkine gene and the manufacturing process of the yeast expression vector,

제3도는 본 발명의 인간 미드카인 발현벡터(pYLBC-A/G-UB-MK)를 이용하여 인간 미드카인을 발현시킨 후 SDS-폴리아크릴 아마이드 젤 전기영동한 결과를 나타낸 것이다.3 shows the results of SDS-polyacrylamide gel electrophoresis after expressing human midkine using the human midkine expression vector (pYLBC-A / G-UB-MK) of the present invention.

Claims (5)

유비퀴틴 유전자에 인간 미드카인 유전자가 융합된 재조합 유전자를 포함하는 발현벡터.An expression vector comprising a recombinant gene in which a human midkine gene is fused to a ubiquitin gene. 제1항에 있어서, pYLBC-A/G-UB-MK인 발현벡터.The expression vector according to claim 1, which is pYLBC-A / G-UB-MK. 제1항 또는 제2항의 발현벡터로 형질전환된 효모.A yeast transformed with the expression vector of claim 1. 제3항에 있어서, 사카로마이세스 세레비지애(Saccharomyces cerevisiae) DC04-UB-MK, pYLBC-A/G-UB-MK(기탁번호:ATCC 74259)인 효모.The yeast of Claim 3 which is Saccharomyces cerevisiae DC04-UB-MK, pYLBC-A / G-UB-MK (Accession Number: ATCC 74259). 제3항 또는 제4항의 효모를 배양시킴을 포함하는, 인간 미드카인 단백질의 제조방법.A method for producing a human midkine protein, comprising culturing the yeast of claim 3 or 4. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940001179A 1994-01-24 1994-01-24 Expression vector of human midkine gene KR100260633B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940001179A KR100260633B1 (en) 1994-01-24 1994-01-24 Expression vector of human midkine gene

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940001179A KR100260633B1 (en) 1994-01-24 1994-01-24 Expression vector of human midkine gene

Publications (2)

Publication Number Publication Date
KR950023720A true KR950023720A (en) 1995-08-18
KR100260633B1 KR100260633B1 (en) 2000-07-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940001179A KR100260633B1 (en) 1994-01-24 1994-01-24 Expression vector of human midkine gene

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KR100260633B1 (en) 2000-07-01

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