KR950023716A - Recombinant Human Fibroblast Growth Factor Gene and Its Expression in Yeast - Google Patents

Recombinant Human Fibroblast Growth Factor Gene and Its Expression in Yeast Download PDF

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KR950023716A
KR950023716A KR1019940000648A KR19940000648A KR950023716A KR 950023716 A KR950023716 A KR 950023716A KR 1019940000648 A KR1019940000648 A KR 1019940000648A KR 19940000648 A KR19940000648 A KR 19940000648A KR 950023716 A KR950023716 A KR 950023716A
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South Korea
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yeast
growth factor
fibroblast growth
human fibroblast
gene
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KR1019940000648A
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Korean (ko)
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KR100312536B1 (en
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조중명
양재영
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성재갑
주식회사 엘지화학
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
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  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 효모에서 대량 발현되도록 변형된 인간 섬유신경세포 성장인자 유전자, 상기 유전자를 포함하는 발현벡터, 상기 발현벡터로 형질전환된 효모 및 이를 배양함을 포함하는 인간 섬유신경세포 성장인자 단백질을 대량 생산하는 방법을 제공한다.The present invention provides a large amount of human fibroblast growth factor gene modified to be expressed in yeast, an expression vector comprising the gene, yeast transformed with the expression vector and a human fibroblast growth factor protein comprising culturing the same. Provide a way to produce.

Description

재조합 인간 섬유 신경세포 성장인자 유전자 및 효모에서의 그의 발현Recombinant Human Fibroblast Growth Factor Gene and Its Expression in Yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 효모내에서의 발현을 목적으로 고안된 유비퀴틴에 융합된 인간 섬유신경세포 성장인자의 뉴크레오티드 서열과 클로닝 목적으로 부가된 뉴클레오티드 서열을 포함하는 전체 유전자의 뉴클레오티드 서열 및 아미노산 서열을 나타낸 것이고,1 shows the nucleotide sequence and amino acid sequence of the entire gene, including the nucleotide sequence of human fibroblast growth factor fused to ubiquitin designed for expression in yeast and the nucleotide sequence added for cloning,

제2도는 효모 발현 운반체로의 클로닝 과정을 도시한 것이고,2 shows the cloning process into yeast expression carriers,

제3도는 인간 섬유신경세포 성장인자의 발현 벡터 pYLBC A/G UB-CNTF를 함유하는 효모의 파쇄액을 변성 폴리아크릴 아마이드젤 전기영동한 결과를 나타낸 것이다.Figure 3 shows the result of denatured polyacrylamide gel electrophoresis of the lysate of yeast containing the expression vector pYLBC A / G UB-CNTF of human fibroblast growth factor.

Claims (7)

유비퀴틴 유전자의 3′ 말단에 효모가 선호하는 코돈을 갖도록 뉴클레오티드 서열을 변형시킨 인간 섬유신경세포 성장인자 유전자를 하나의 오픈 리딩 프레임으로 연결시킨 제조합 유전자.A synthetic gene in which the human fibroblast growth factor gene, in which the nucleotide sequence is modified to have a codon preferred by the yeast at the 3 ′ end of the ubiquitin gene, is linked to one open reading frame. 제1항에 있어서, 상기 인간 섬유신경세포 성장인자 유전자가 하기 뉴클레오티드 서열을 갖는 제조합 유전자:The synthetic gene of claim 1, wherein the human fibroblast growth factor gene has the following nucleotide sequence: 제1항 또는 제2항의 유전자를 포함하는 발현 벡터.An expression vector comprising the gene of claim 1 or 2. 제1항에 있어서, pYLBC-A/G-UB-CNTF인 발현벡터.The expression vector according to claim 1, which is pYLBC-A / G-UB-CNTF. 제3항 또는 제4항의 발현 벡터로 형질전환된 효모.Yeast transformed with the expression vector of claim 3. 제5항에 있어서, 사카로마이세스 세레비지애(Saccharomyces cerevisiae) DCO4-UB-CNTF, pYLBC-A/G-UV-CNTF(기탁번호:ATCC 74258)인 효모.The yeast of claim 5 which is Saccharomyces cerevisiae DCO4-UB-CNTF, pYLBC-A / G-UV-CNTF (Accession Number: ATCC 74258). 제5항 또는 제6항의 효모를 배양시킴을 포함하는, 인간 섬유신경세포성장인자 단백질의 제조방법.A method of producing a human fibroblast growth factor protein, comprising culturing the yeast of claim 5 or 6. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940000648A 1994-01-15 1994-01-15 Gene encoding recombinant human ciliary neurotrophic factor(cntf) and expression thereof in yeast KR100312536B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940000648A KR100312536B1 (en) 1994-01-15 1994-01-15 Gene encoding recombinant human ciliary neurotrophic factor(cntf) and expression thereof in yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940000648A KR100312536B1 (en) 1994-01-15 1994-01-15 Gene encoding recombinant human ciliary neurotrophic factor(cntf) and expression thereof in yeast

Publications (2)

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KR950023716A true KR950023716A (en) 1995-08-18
KR100312536B1 KR100312536B1 (en) 2002-06-20

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KR1019940000648A KR100312536B1 (en) 1994-01-15 1994-01-15 Gene encoding recombinant human ciliary neurotrophic factor(cntf) and expression thereof in yeast

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