KR930013109A - Expression of Modified Human Macrophage Colony Stimulating Factor Gene - Google Patents

Expression of Modified Human Macrophage Colony Stimulating Factor Gene Download PDF

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Publication number
KR930013109A
KR930013109A KR1019910025880A KR910025880A KR930013109A KR 930013109 A KR930013109 A KR 930013109A KR 1019910025880 A KR1019910025880 A KR 1019910025880A KR 910025880 A KR910025880 A KR 910025880A KR 930013109 A KR930013109 A KR 930013109A
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South Korea
Prior art keywords
macrophage colony
stimulating factor
colony stimulating
human macrophage
modified human
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KR1019910025880A
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Korean (ko)
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KR940005596B1 (en
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박영우
조중명
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최근선
주식회사 럭키
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract

본 발명은 변형된 인간 거식세포 군체 작극 인자 유전자, 이를 포함하는 효모용 발현 벡터, 상기 발현 벡터에 의해 형질전환된 효모 및 이를 이용한 인간 거식 세포 군체 자극 인자의 제조방법을 제공한다.The present invention provides a modified human macrophage colony activating factor gene, an expression vector for yeast comprising the same, yeast transformed by the expression vector and a method for producing human macrophage colony stimulating factor using the same.

Description

변형된 인간 거식 세포군체 자극 인자 유전자의 발현Expression of Modified Human Macrophage Cell Stimulation Factor Gene

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1-a및 제1-b도는 변형된 인간 GM-CSF 유전자의 염기 서열및 아미노산 서열을 나타낸 것이고,Figures 1-a and 1-b show the nucleotide sequence and amino acid sequence of the modified human GM-CSF gene,

제2도는 알파 인자 리더 염기 서열 및 아미노산 서열을 나탄낸 것이고,2 shows the alpha factor leader nucleotide sequence and amino acid sequence,

제3도는 본 발명에 따른 효모 발현 운반체로의 클로닝 과정을 도시한 것이고,3 illustrates the cloning process into a yeast expression carrier according to the invention,

제4도는 본 발명의 GM-CSF발현 벡터를 함유하는 효모의 농축배양액을 SDS-폴리아크릴 아마이드 겔 전기영동한 결과를 나타낸 것이다.Figure 4 shows the results of SDS-polyacrylamide gel electrophoresis of the concentrated culture solution of yeast containing the GM-CSF expression vector of the present invention.

Claims (9)

아미노산 치환에 의해 2개의 N-당화 인식 부위가 제거된 변형된 인간 거식세포 군체 자극 인자 유전자.A modified human macrophage colony stimulating factor gene wherein two N-glycosylation recognition sites have been removed by amino acid substitutions. 제1항에 있어서, 제1-a도 또는 제1-b도에 도시한 아미노산 서열을 암호화 하는 변형된 인간 거식세포 군체 자극 인자 유전자.The modified human macrophage colony stimulating factor gene according to claim 1, which encodes the amino acid sequence shown in FIGS. 1-a or 1-b. 제1항 또는 제2항의 유전자에 의해 암호화 되는 인간 거식세포 군체 자극 인자.A human macrophage colony stimulating factor encoded by the gene of claim 1. 제1항 또는 제2항의 유전자를 포함하여 제조된 효모용 발현 벡터.An expression vector for yeast, comprising the gene of claim 1 or 2. 제4항에 있어서, 발현 벡터 LUCK-GM-CSF-1Y.The expression vector LUCK-GM-CSF-1Y of claim 4. 제4항에 있어서, 발현 벡터 LUCK-GM-CSF-2Y.The expression vector LUCK-GM-CSF-2Y according to claim 4. 제4항 내지 제6항중 어느 한 항의 발현 벡터를 의해 형질 전환된 효모.A yeast transformed with the expression vector of any one of claims 4 to 6. 제7항에 있어서, Saccharomyces cerevisiae (LUCK-GM-CSF-1Y)(기탁번호 KFCC 10716).Saccharomyces cerevisiae (LUCK-GM-CSF-1Y) according to claim 7 (Accession No. KFCC 10716). 제7항 또는 제8항의 효모를 이용하여 변형된 인간 거식세포 군체 자극 인자를 제조하는 방법.A method for preparing a modified human macrophage colony stimulating factor using the yeast of claim 7. ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.※ Note: This is to be disclosed by the original application.
KR1019910025880A 1991-12-31 1991-12-31 Gene disclose a granulocyte macrophage colony stimulating factor KR940005596B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910025880A KR940005596B1 (en) 1991-12-31 1991-12-31 Gene disclose a granulocyte macrophage colony stimulating factor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910025880A KR940005596B1 (en) 1991-12-31 1991-12-31 Gene disclose a granulocyte macrophage colony stimulating factor

Publications (2)

Publication Number Publication Date
KR930013109A true KR930013109A (en) 1993-07-21
KR940005596B1 KR940005596B1 (en) 1994-06-21

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Application Number Title Priority Date Filing Date
KR1019910025880A KR940005596B1 (en) 1991-12-31 1991-12-31 Gene disclose a granulocyte macrophage colony stimulating factor

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