KR950008688A - Genes Encoding Glucose Enzyme II of Brevibacterium lactofermentum and Expression thereof - Google Patents

Genes Encoding Glucose Enzyme II of Brevibacterium lactofermentum and Expression thereof Download PDF

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Publication number
KR950008688A
KR950008688A KR1019930020230A KR930020230A KR950008688A KR 950008688 A KR950008688 A KR 950008688A KR 1019930020230 A KR1019930020230 A KR 1019930020230A KR 930020230 A KR930020230 A KR 930020230A KR 950008688 A KR950008688 A KR 950008688A
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South Korea
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gene
glucose enzyme
expression
brevibacterium lactofermentum
brevibacterium
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KR1019930020230A
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Korean (ko)
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KR0176412B1 (en
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윤기홍
오태광
이정기
이규남
권일
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김은영
한국과학기술연구원
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Abstract

본 발명은 브레비박테리움 락토퍼멘텀의 글루코스 효소 Ⅱ를 코드하는 유전자, 상기 유전자에 의해 코드되는 글루코스 효소및 미생물에서의 상기 유전자의 발현에 관한 것이다.The present invention provides a gene encoding glucose enzyme II of Brevibacterium lactofermentum, a glucose enzyme encoded by the gene. And expression of said gene in a microorganism.

Description

브레비박테리움 락토퍼멘텀의 글루코스 효소 를 코드하는 유전자 및 이의 발현Genes Encoding Glucose Enzymes of Brevibacterium Lactofermentum and Their Expression

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따라 클로닝된 브레비박테리움 락토퍼멘텀의 글루코스 효소 Ⅱ를 코드하는 유전자의 염기서열을 나타낸 것이고,Figure 1 shows the nucleotide sequence of the gene encoding the glucose enzyme II of Brevibacterium lactofermentum cloned according to the present invention,

제2도는 제1도의 유전자 염기서열로부터 유추된 브레비박테리움 락토퍼멘텀의 글루코스효소 Ⅱ의 아미노산 잔기 서열을 나타낸 것이고,Figure 2 shows the amino acid residue sequence of glucose enzyme II of Brevibacterium lactofermentum inferred from the gene sequence of Figure 1,

제3도는 제1도의 유전자를 포함하는 3163bp 크기의 DNA 삽입단편을 함유한 재조합 플라스미드의 제한효소지도이다.3 is a restriction map of a recombinant plasmid containing a 3163 bp DNA insert fragment containing the gene of FIG.

Claims (8)

브레비박테리움 락토퍼맨텀의 글루코스 효소 Ⅱ를 코드하는 유전자.A gene encoding glucose enzyme II of Brevibacterium lactofermantum. 제1항에 있어서, 다음의 염기서열을 갖는 유전자.The gene of claim 1, wherein the gene has the following nucleotide sequence. 다음의 아미노산 잔기 서열을 갖는 브레비박테리움 락토퍼맨텀의 글루코스 효소 Ⅱ.Glucose enzyme II of Brevibacterium lactofermentum having the following amino acid residue sequence. 제1항 또는 제2항의 유전자를 포함하는 발현 벡터.An expression vector comprising the gene of claim 1 or 2. 제4항에 있어서, 재조합 플라스키드 pBSG2(KCTC 8541P).The recombinant plasma pBSG2 (KCTC 8541P) according to claim 4. 제4항의 재조합 플라스미드로 형질전환된 미생물.Microorganism transformed with the recombinant plasmid of claim 4. 제6항에 있어서, 대장균 ASC113/pBSG2(KCTC 8541P).The E. coli ASC113 / pBSG2 (KCTC 8541P) according to claim 6. 브레비박테리움 락토퍼멘텀의 글루코스효소 Ⅱ를 코드하는 유전자를 포함하는 발현벡터에 의해 형질전환된 미생물을, 상기 유전자의 발현을 가능하게 하는 조건하에서 배양함을 포함하는, 브레비박테리움 락토퍼멘텀의 글루코스 효소 Ⅱ를 제조하는 방법.Brevibacterium lactopper, comprising culturing a microorganism transformed with an expression vector comprising a gene encoding the glucose enzyme II of Brevibacterium lactofermentum under conditions enabling the expression of said gene. Method for preparing Menth's Glucose Enzyme II. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019930020230A 1993-09-28 1993-09-28 Genes Encoding Glucose Enzyme II of Brevibacterium lactofermentum and Expression thereof KR0176412B1 (en)

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KR1019930020230A KR0176412B1 (en) 1993-09-28 1993-09-28 Genes Encoding Glucose Enzyme II of Brevibacterium lactofermentum and Expression thereof

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KR1019930020230A KR0176412B1 (en) 1993-09-28 1993-09-28 Genes Encoding Glucose Enzyme II of Brevibacterium lactofermentum and Expression thereof

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KR950008688A true KR950008688A (en) 1995-04-19
KR0176412B1 KR0176412B1 (en) 1999-04-01

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