KR940021734A - Gene encoding the mannose enzyme II of Corynebacterium glutamicum and its expression - Google Patents

Gene encoding the mannose enzyme II of Corynebacterium glutamicum and its expression Download PDF

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KR940021734A
KR940021734A KR1019930003655A KR930003655A KR940021734A KR 940021734 A KR940021734 A KR 940021734A KR 1019930003655 A KR1019930003655 A KR 1019930003655A KR 930003655 A KR930003655 A KR 930003655A KR 940021734 A KR940021734 A KR 940021734A
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corynebacterium glutamicum
mannose enzyme
gene encoding
expression
enzyme
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KR960008410B1 (en
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이정기
성문희
윤기홍
오태광
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서정욱
한국과학기술연구원
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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Abstract

본 발명은 제1도에 나타낸 바와같은 아미노산 서열을 갖는 신규의 코리네박테리움 글루타미컴의 만노스효소 Ⅱ, 이를 코드하는 유전자와 그를 함유하는 발현 플라스미드, 그 발현 플라스미드로 형질전환된 대장균 세포 그 형질전환된 대장균 세포를 배양하여 만노스 효소 Ⅱ를 생산하는 방법을 제공한다.The present invention relates to a novel Corynebacterium glutamicum mannose enzyme II having an amino acid sequence as shown in FIG. Provided is a method of culturing converted E. coli cells to produce mannose enzyme II.

Description

코리네박테리움 글루타미컴(Corynebacterium glutamicum)의 만노스 효소 Ⅱ를 코드하는 유전자와 그의 발현Gene encoding the mannose enzyme II of Corynebacterium glutamicum and its expression

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따라 클로닝된 코리네박테리움 글루타미컴의 만노스 효소 Ⅱ를 코드하는 유전자의 염기서열 및 그로부터 유추된 아미노산 서열을 개시한 것이고;1 discloses the nucleotide sequence of a gene encoding the mannose enzyme II of Corynebacterium glutamicum cloned according to the present invention and the amino acid sequence deduced therefrom;

제2도는 제1도의 유전자를 포함하는 2177bp의 DNA삽입단편의 제한 효소 지도이며;FIG. 2 is a restriction map of the DNA insertion fragment of 2177 bp containing the gene of FIG. 1;

제3도는 클론한 2177bp의 염기서열을 밝히기 위한 염기서열 결정 방법이다.3 is a method for determining the nucleotide sequence to identify the cloned nucleotide sequence of 2177bp.

Claims (8)

다음의 아미노산 서열을 갖는 코리네박테리움 글루타미컴Corynebacterium glutamicum having the following amino acid sequence (Corynebacterium glutamicum)의 만노스 효소 Ⅱ.(Corynebacterium glutamicum) mannose enzyme II. 제1항의 만노스 효소 Ⅱ를 코드하는 유전자.The gene encoding the mannose enzyme II of claim 1. 제2항에 있어서, 다음의 DNA염기서열을 갖는 유전자.The gene of claim 2, wherein the gene has the following DNA base sequence. 제2항 또는 제3항의 유전자를 가지는 발현 플라스미드.An expression plasmid having the gene of claim 2 or 3. 제4항에 있어서, 재조합 플라스미드 pCTS3(KCTC 8527P).The recombinant plasmid pCTS3 (KCTC 8527P) according to claim 4. 제4항 또는 제5항의 재조합 플라스미드로 형질전환된 대장균.E. coli transformed with the recombinant plasmid of claim 4 or 5. 제6항에 있어서, 대장균 ZSC113(pCTS3).The E. coli ZSC113 (pCTS3) according to claim 6. 제6항 또는 제7항의 대장균을 배양함을 포함하는 코리네박테리움 글루타미컴의 만노스 효소 Ⅱ의 생산방법.A method for producing mannose enzyme II of Corynebacterium glutamicum comprising culturing E. coli according to claim 6 or 7. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR93003655A 1993-03-11 1993-03-11 Gene and expression of mannose enzyme from corynebacterium glutamicum KR960008410B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR93003655A KR960008410B1 (en) 1993-03-11 1993-03-11 Gene and expression of mannose enzyme from corynebacterium glutamicum

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR93003655A KR960008410B1 (en) 1993-03-11 1993-03-11 Gene and expression of mannose enzyme from corynebacterium glutamicum

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KR940021734A true KR940021734A (en) 1994-10-19
KR960008410B1 KR960008410B1 (en) 1996-06-26

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