KR940007184A - Straptokinase Gene and Its Expression - Google Patents

Straptokinase Gene and Its Expression Download PDF

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Publication number
KR940007184A
KR940007184A KR1019920017406A KR920017406A KR940007184A KR 940007184 A KR940007184 A KR 940007184A KR 1019920017406 A KR1019920017406 A KR 1019920017406A KR 920017406 A KR920017406 A KR 920017406A KR 940007184 A KR940007184 A KR 940007184A
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South Korea
Prior art keywords
gene
streptokinase
expression vector
expression
straptokinase
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KR1019920017406A
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Korean (ko)
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KR950012901B1 (en
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조중명
박영우
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최근선
주식회사 럭 키
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Organic Chemistry (AREA)
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  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 제1도에 나타낸 바와같은 새로운 스트렙토키나제 유전자와 그를 함유하는 발현벡터, 그러한 발현벡터로 형질전환된 미생물 및 그 미생물을 배양하여 스트렙토키나제를 생산하는 방법을 제공하는 것이다.The present invention provides a novel streptokinase gene, as shown in FIG.

Description

스트렙토키나제 유전자 및 그의 발현Streptokinase Gene and Its Expression

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명의 스트렙토키나제 유전자의 염기서열 및 이로 부터 유추된 아미노산 서열이고,1 is a nucleotide sequence of the streptokinase gene of the present invention and an amino acid sequence deduced therefrom,

제2도는 본 발명이 스트렙토키나제 유전자로부터 유추된 아미노산 서열을 기존의 다른 스트렙토키나제와 비교하여 나타낸 것이며,Figure 2 shows the amino acid sequence inferred from the streptokinase gene of the present invention compared to other existing streptokinase,

제3도는 스트렙토키나제 유전자의 클로닝 과정을 도시한 것이고,3 shows the cloning process of the streptokinase gene,

제4도는 스트렙토키나제 발현벡터의 변성 폴리아크릴아마이드 젤 전기영동한 결과를 나타낸 것이다.Figure 4 shows the results of the electrophoresis of modified polyacrylamide gel of the streptokinase expression vector.

Claims (7)

하기 아미노산 서열을 갖는 신규한 스트렙토키나제를 코딩하는 염기서열을 포함하는 스트렙토키나제 유전자.A streptokinase gene comprising a nucleotide sequence encoding a novel streptokinase having the following amino acid sequence. 제1항에 있어서, 다음의 염기 서열을 갖는 신규한 스트렙토키나제 유전자.The novel streptokinase gene of claim 1, having the following nucleotide sequence. 제1항 또는 제2항의 유전자를 함유하는 스트렙토카나제 발현 벡터.A streptocanase expression vector containing the gene of claim 1. 제3항에 있어서, 발현벡터 ptrpH-SK.The expression vector ptrpH-SK according to claim 3. 제3항 또는 제4항의 발현벡터로 형질전환된 대장균.E. coli transformed with the expression vector of claim 3 or 4. 제5항에 있어서, ptrpH-SK에 의해 형질전환된 대장균 W3110(ATCC 68884).E. coli W3110 (ATCC 68884), according to claim 5, transformed with ptrpH-SK. 제5항 또는 제6항의 대장균을 배양하여서 스트렙토키나제를 생산하는 방법.A method for producing streptokinase by culturing Escherichia coli according to claim 5 or 6. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920017406A 1992-09-24 1992-09-24 Gene & expression for streptokinase KR950012901B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019920017406A KR950012901B1 (en) 1992-09-24 1992-09-24 Gene & expression for streptokinase

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920017406A KR950012901B1 (en) 1992-09-24 1992-09-24 Gene & expression for streptokinase

Publications (2)

Publication Number Publication Date
KR940007184A true KR940007184A (en) 1994-04-26
KR950012901B1 KR950012901B1 (en) 1995-10-23

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KR1019920017406A KR950012901B1 (en) 1992-09-24 1992-09-24 Gene & expression for streptokinase

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KR950012901B1 (en) 1995-10-23

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