KR930008146A - Sheep growth hormone gene and its expression - Google Patents

Sheep growth hormone gene and its expression Download PDF

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Publication number
KR930008146A
KR930008146A KR1019910017583A KR910017583A KR930008146A KR 930008146 A KR930008146 A KR 930008146A KR 1019910017583 A KR1019910017583 A KR 1019910017583A KR 910017583 A KR910017583 A KR 910017583A KR 930008146 A KR930008146 A KR 930008146A
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South Korea
Prior art keywords
growth hormone
sheep growth
hormone gene
expression
coli
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KR1019910017583A
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Korean (ko)
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KR940005593B1 (en
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조중명
박영우
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최근선
주식회사 럭키
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Publication of KR940005593B1 publication Critical patent/KR940005593B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 유전공학적인 방법으로 양 성장호르몬 유전자를 생산하는데 유용하도록 설계된 양 성장호르몬 유전자와 이러한 양 성장호르몬을 대장균을 발현체로하여 발현시키는데 적합한 양 성장호르몬 발현 벡터, 그에 의해 형질전환된 대장균 및 이를 이용한 양 성장호르몬 기조방법에 관한 것이다.The present invention provides a sheep growth hormone gene designed to be useful for producing a sheep growth hormone gene by genetic engineering method, and a sheep growth hormone expression vector suitable for expressing such sheep growth hormone as E. coli as an expression, E. coli transformed by The present invention relates to a growth hormone keynote method used.

Description

양 성장호르몬 유전자 및 그의 발현Sheep growth hormone gene and its expression

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 본 발명에 따라 설계된 양 성장호르몬 유전자의 염기 서열과 이로부터 유추된 아미노산서열을 나타낸 것이고,Figure 1 shows the nucleotide sequence of the sheep growth hormone gene designed according to the present invention and the amino acid sequence deduced from it,

제2도는 제1도의 양 성장호르몬 유전자를 합성하기 위한 전략을 도시한 것이고,2 shows a strategy for synthesizing both growth hormone genes of FIG.

제3도는 본 발명에 따른 양 성장호르몬 유전자를 대장균 발현 벡터로 클로닝하는 과정을 도시한 것이다.Figure 3 shows the procedure for cloning the sheep growth hormone gene according to the present invention E. coli expression vector.

Claims (7)

제1도의 염기 서열을 갖는 양 성장호르몬 유전자.Sheep growth hormone gene having the nucleotide sequence of FIG. 제1항의 양 성장호르몬 유전자를 함유하는 발현벡터.An expression vector containing the sheep growth hormone gene of claim 1. 제2항에 있어서, ptrp-OST.The ptrp-OST of claim 2. 제2항 또는 제3항에 따르는 발현 벡터에 의해 형질전환된 대장균.E. coli transformed with the expression vector according to claim 2. 제4항에 있어서, ptrp-OST에 의해 형질전환된 대장균 E. coli W3110(KCCM-10006).The Escherichia coli E. coli W3110 (KCCM-10006) transformed by ptrp-OST. 제4항 또는 제5항의 대장균을 이용하여 양 성장호르몬을 제조하는 방법.A method for producing sheep growth hormone using E. coli of claim 4 or 5. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019910017583A 1991-10-08 1991-10-08 Revelation and gene of o-growth hormones KR940005593B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019910017583A KR940005593B1 (en) 1991-10-08 1991-10-08 Revelation and gene of o-growth hormones

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019910017583A KR940005593B1 (en) 1991-10-08 1991-10-08 Revelation and gene of o-growth hormones

Publications (2)

Publication Number Publication Date
KR930008146A true KR930008146A (en) 1993-05-21
KR940005593B1 KR940005593B1 (en) 1994-06-21

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Application Number Title Priority Date Filing Date
KR1019910017583A KR940005593B1 (en) 1991-10-08 1991-10-08 Revelation and gene of o-growth hormones

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KR940005593B1 (en) 1994-06-21

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