KR950023718A - Human myocyte growth factor - Google Patents

Human myocyte growth factor Download PDF

Info

Publication number
KR950023718A
KR950023718A KR1019940001145A KR19940001145A KR950023718A KR 950023718 A KR950023718 A KR 950023718A KR 1019940001145 A KR1019940001145 A KR 1019940001145A KR 19940001145 A KR19940001145 A KR 19940001145A KR 950023718 A KR950023718 A KR 950023718A
Authority
KR
South Korea
Prior art keywords
growth factor
myocyte growth
gene
human myocyte
human
Prior art date
Application number
KR1019940001145A
Other languages
Korean (ko)
Other versions
KR100339151B1 (en
Inventor
조중명
김천형
Original Assignee
성재갑
주식회사 엘지화학
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 성재갑, 주식회사 엘지화학 filed Critical 성재갑
Priority to KR1019940001145A priority Critical patent/KR100339151B1/en
Publication of KR950023718A publication Critical patent/KR950023718A/en
Application granted granted Critical
Publication of KR100339151B1 publication Critical patent/KR100339151B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 인간 근세포 성장인자의 유전자, 그 유전자를 포함하는 플라스미드 및 그 플라스미드로 형질전환된 형질전환체에 관한 것으로, 본 발명의 형질전환체를 배양하여 얻어진 인간 근세포 성장인자는 조혈작용의 장애로 인한 빈형, 백혈병 등의 치료와 골수 이식 등에 사용될 수 있다.The present invention relates to a gene of human myocyte growth factor, a plasmid containing the gene and a transformant transformed with the plasmid, wherein the human myocyte growth factor obtained by culturing the transformant of the present invention is a disorder of hematopoietic action. It can be used for the treatment of hepatitis, leukemia, etc. and bone marrow transplantation.

Description

인간 근세포 성장인자Human myocyte growth factor

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 인간 근세포 성장인자 유전자의 염기서열과 아미노산 서열을 나타낸 것이고,Figure 1 shows the nucleotide sequence and amino acid sequence of the human myocyte growth factor gene,

제2도는 합성한 인간 근세포 성장인자 유전자들의 접합 전략 및 발현 벡터를 도시한 설명도이고,2 is an explanatory diagram showing the conjugation strategy and expression vector of the synthesized human myocyte growth factor genes,

제3도는 인간 근세포 성장인자 유전자의 발현을 SDS 폴리 아크릴아마이드 젤 전기영동 사진이다.3 is a SDS polyacrylamide gel electrophoresis picture of the expression of the human myocyte growth factor gene.

Claims (6)

인간 근세포 성장인자를 암호화하며, 염기서열 전체에 걸쳐 핵산의 자유 에너지 값(G)이-7Kcal/mol 이하임을 특징으로 하는 제1도에 표시된 근세포 성장인자의 유전자.A gene of the myocyte growth factor shown in FIG. 1, which encodes a human myocyte growth factor, wherein the free energy value (G) of the nucleic acid is less than -7 Kcal / mol throughout the nucleotide sequence. 제1항의 유전자를 포함하는 플라스미드.A plasmid comprising the gene of claim 1. 제2항에 있어서, 플라스미드 ptrpH-UB-SCF 또는 ptrpH-SCF.The plasmid ptrpH-UB-SCF or ptrpH-SCF according to claim 2. 제1항의 유전자를 포함하는 발현벡터에 의해 형질전환된 미생물.Microorganisms transformed by an expression vector comprising the gene of claim 1. 제4항에 있어서, Eschericha colil W3110(ptrp-VB-hSCF) 또는 Escherichia colil W3110(pTrp-SCF)(ATT 69512)임을 특징으로 하는 형질전환체.The transformant of claim 4, which is Eschericha colil W3110 (ptrp-VB-hSCF) or Escherichia colil W3110 (pTrp-SCF) (ATT 69512). 제4항의 형질전환체를 배양하여 얻어진 인간 근세포 성장인자.Human myocyte growth factor obtained by culturing the transformant of claim 4. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019940001145A 1994-01-22 1994-01-22 Human myoblast growth factor KR100339151B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019940001145A KR100339151B1 (en) 1994-01-22 1994-01-22 Human myoblast growth factor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019940001145A KR100339151B1 (en) 1994-01-22 1994-01-22 Human myoblast growth factor

Publications (2)

Publication Number Publication Date
KR950023718A true KR950023718A (en) 1995-08-18
KR100339151B1 KR100339151B1 (en) 2002-11-23

Family

ID=37480150

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019940001145A KR100339151B1 (en) 1994-01-22 1994-01-22 Human myoblast growth factor

Country Status (1)

Country Link
KR (1) KR100339151B1 (en)

Also Published As

Publication number Publication date
KR100339151B1 (en) 2002-11-23

Similar Documents

Publication Publication Date Title
AU7833494A (en) Enhanced expression in plants using non-translated leader sequences
NZ312332A (en) Recombinational cloning using engineered recombination sites
DE69836092D1 (en) RECOMBINATORY CLONING USING NUCLEAR FACILITIES WITH RECOMBINATION CENTERS
NO20015587L (en) Expression and export of interferon <alpha> proteins such as Fc fusion proteins
ES2177475T1 (en) GENES OF CORYNEBACTERIUM GLUTAMICUM CODING PROTEINS OF A METABOLIC ROUTE.
ATE310102T1 (en) HIGH RESOLUTION DNA SEQUENCING USING A LOW VISCOSITY MEDIUM
EP1005529A4 (en) Methods for lyophilizing competent cells
KR920012450A (en) Method for preparing D-pantothenic acid and plasmids and microorganisms for producing the same
KR900009981A (en) Coding genes and gene constructs of aminotransferases, and microorganisms expressing these genes
LU88360I2 (en) Leucomax
DE69834565D1 (en) FAST GENERATION OF STABLE MAMMALIAN ANIMAL CELL LINES WITH RECOVERY OF HIGH QUANTITY OF RECOMBINANT PROTEINS
DE69504808D1 (en) CORTICOTROPIN RELEASING RECEPTORS OF FACTOR 2
EA200100583A1 (en) COMPOSITIONS CONTAINING LACTADGERIN OR ITS OPTIONS AND METHODS OF THEIR USE
KR950023718A (en) Human myocyte growth factor
DK0596881T3 (en) Expression in non-tumoral human lymphoblastoids with an integrative vector
DE59712771D1 (en) Epididymis-specific receptor protein and its use
ATE43139T1 (en) GAMMA INTERFERON CLONING AND EXPRESSION VECTORS, TRANSFORMED BACTERIA AND METHODS OF GAMMA INTERFERON PRODUCTION.
KR950005990A (en) Expression of human interleukin-4 in yeast
KR940021732A (en) Expression of the Nonstructural 4-Part Protein of Hepatitis C Virus
KR950005989A (en) Expression of human interleukin-3 in yeast
KR940007184A (en) Straptokinase Gene and Its Expression
KR960014347A (en) Overexpressed Recombinant Vectors of Escherichia Coli and Methods of Preparing Phospholipocyte Colony Stimulating Factor Using the Same
KR940021734A (en) Gene encoding the mannose enzyme II of Corynebacterium glutamicum and its expression
Blum Cilia and Flagella, Michael A. Sleigh (Ed.), Academic Press, Ann Arbor, Michican (1974)
JPS56131598A (en) Novel recombinant plasmid having gene of human fibroblastic interferon messenger rna

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
LAPS Lapse due to unpaid annual fee