DE69834565D1 - FAST GENERATION OF STABLE MAMMALIAN ANIMAL CELL LINES WITH RECOVERY OF HIGH QUANTITY OF RECOMBINANT PROTEINS - Google Patents

FAST GENERATION OF STABLE MAMMALIAN ANIMAL CELL LINES WITH RECOVERY OF HIGH QUANTITY OF RECOMBINANT PROTEINS

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Publication number
DE69834565D1
DE69834565D1 DE69834565T DE69834565T DE69834565D1 DE 69834565 D1 DE69834565 D1 DE 69834565D1 DE 69834565 T DE69834565 T DE 69834565T DE 69834565 T DE69834565 T DE 69834565T DE 69834565 D1 DE69834565 D1 DE 69834565D1
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DE
Germany
Prior art keywords
cell lines
recombinant proteins
recovery
animal cell
stable mammalian
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69834565T
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German (de)
Other versions
DE69834565T2 (en
Inventor
Kathleen Tomkinson
Monique Davies
John Mccoy
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Genetics Institute LLC
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Genetics Institute LLC
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Publication date
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Publication of DE69834565D1 publication Critical patent/DE69834565D1/en
Publication of DE69834565T2 publication Critical patent/DE69834565T2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16634—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Abstract

Disclosed are methods, DNA sequences, vectors and cell lines useful for the rapid generation of stable mammalian cell lines expressing high levels of recombinant proteins.
DE69834565T 1997-10-29 1998-10-21 FAST GENERATION OF STABLE MAMMALIAN ANIMAL CELL LINES WITH RECOVERY OF HIGH QUANTITY OF RECOMBINANT PROTEINS Expired - Lifetime DE69834565T2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US6344997P 1997-10-29 1997-10-29
US63449P 1997-10-29
US175690 1998-10-20
US09/175,690 US6136536A (en) 1997-10-29 1998-10-20 Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins
PCT/US1998/022231 WO1999021976A2 (en) 1997-10-29 1998-10-21 Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins

Publications (2)

Publication Number Publication Date
DE69834565D1 true DE69834565D1 (en) 2006-06-22
DE69834565T2 DE69834565T2 (en) 2007-04-26

Family

ID=26743435

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69834565T Expired - Lifetime DE69834565T2 (en) 1997-10-29 1998-10-21 FAST GENERATION OF STABLE MAMMALIAN ANIMAL CELL LINES WITH RECOVERY OF HIGH QUANTITY OF RECOMBINANT PROTEINS

Country Status (12)

Country Link
US (1) US6136536A (en)
EP (1) EP1025245B1 (en)
JP (2) JP2001520882A (en)
AT (1) ATE326537T1 (en)
AU (1) AU740768B2 (en)
CA (1) CA2306449C (en)
CY (1) CY1105290T1 (en)
DE (1) DE69834565T2 (en)
DK (1) DK1025245T3 (en)
ES (1) ES2263225T3 (en)
PT (1) PT1025245E (en)
WO (1) WO1999021976A2 (en)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
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WO2004083378A2 (en) * 2003-03-17 2004-09-30 Emory University Mammalian cell lines specifically deficient in o-linked glycosylation
KR100533794B1 (en) * 2003-10-02 2005-12-07 주식회사 프로젠 Method for mass production of human Follicle Stimulating Hormone
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
EP1687017B1 (en) 2003-10-24 2013-03-06 Gencia Corporation Methods and compositions for delivering polynucleotides
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
CN103215328B (en) 2004-01-21 2016-08-03 诺和诺德医疗保健公司 The joint of transglutaminase mediated peptide
DK2147105T3 (en) 2007-05-02 2013-06-03 Merial Ltd DNA PLASMIDS WITH IMPROVED EXPRESSION AND STABILITY
US20090123494A1 (en) * 2007-07-31 2009-05-14 William Staplin Momlv-based pseudovirion packaging cell line
KR20110039348A (en) * 2008-08-06 2011-04-15 노보 노르디스크 헬스 케어 악티엔게젤샤프트 Conjugated proteins with prolonged in vivo efficacy
AU2010207725B2 (en) 2009-01-22 2015-06-11 Novo Nordisk Health Care Ag Stable growth hormone compounds
CN102612376A (en) 2009-08-06 2012-07-25 诺沃-诺迪斯克保健股份有限公司 Growth hormones with prolonged in-vivo efficacy
WO2011089255A1 (en) 2010-01-22 2011-07-28 Novo Nordisk Health Care Ag Growth hormones with prolonged in-vivo efficacy
RU2012134974A (en) 2010-01-22 2014-02-27 Ново Нордиск Хелс Кеа Аг STABILIZED GROWTH HORMONE COMPOUND
WO2012010516A1 (en) 2010-07-22 2012-01-26 Novo Nordisk Health Care Ag Growth hormone conjugates
EP2981282B1 (en) 2013-04-05 2020-11-04 Novo Nordisk Health Care AG Growth hormone compound formulation
EP3218514B1 (en) * 2014-11-12 2019-07-24 LEK Pharmaceuticals d.d. Predicting genetically stable recombinant protein production in early cell line development

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US5550036A (en) * 1986-04-09 1996-08-27 Eli Lilly And Company Method for co-amplification of human protein C genes in human cells
US5094953A (en) * 1988-03-21 1992-03-10 Genentech, Inc. Human tissue plasminogen activator variants
WO1994016075A2 (en) * 1993-01-13 1994-07-21 Genetics Institute, Inc. Process for producing m-csf 223
DE69420033T2 (en) * 1993-04-06 1999-12-23 Us Gov Health & Human Serv GIBBON LEUKEMIA VIRUS RETROVIRALE VECTORS
US6004941A (en) * 1993-06-14 1999-12-21 Basf Aktiengesellschaft Methods for regulating gene expression
DE705334T1 (en) * 1993-06-14 1999-12-30 Basf Ag STRICT CONTROL OF GENE EXPRESSION IN EUKARYOTIC CELLS BY PROTOTORS REACTING ON TETRAZYKLIN
US5866755A (en) * 1993-06-14 1999-02-02 Basf Aktiengellschaft Animals transgenic for a tetracycline-regulated transcriptional inhibitor
US5464758A (en) * 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
JPH08305625A (en) * 1995-05-11 1996-11-22 Hitachi Ltd Semiconductor memory having arithmetic processing function and processor using the same
US5891718A (en) * 1996-03-27 1999-04-06 Vical Incorporated Tetracycline inducible/repressible systems

Also Published As

Publication number Publication date
DE69834565T2 (en) 2007-04-26
JP2010000087A (en) 2010-01-07
CA2306449A1 (en) 1999-05-06
EP1025245B1 (en) 2006-05-17
AU740768B2 (en) 2001-11-15
ES2263225T3 (en) 2006-12-01
DK1025245T3 (en) 2006-08-21
US6136536A (en) 2000-10-24
WO1999021976A3 (en) 1999-07-08
JP2001520882A (en) 2001-11-06
CA2306449C (en) 2008-12-16
ATE326537T1 (en) 2006-06-15
AU1109199A (en) 1999-05-17
EP1025245A2 (en) 2000-08-09
PT1025245E (en) 2006-08-31
CY1105290T1 (en) 2010-03-03
WO1999021976A2 (en) 1999-05-06

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