KR960041365A - Novel β-1,4 Endoglucanase Gene and Microorganisms Expressing It - Google Patents

Novel β-1,4 Endoglucanase Gene and Microorganisms Expressing It Download PDF

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Publication number
KR960041365A
KR960041365A KR1019960042889A KR19960042889A KR960041365A KR 960041365 A KR960041365 A KR 960041365A KR 1019960042889 A KR1019960042889 A KR 1019960042889A KR 19960042889 A KR19960042889 A KR 19960042889A KR 960041365 A KR960041365 A KR 960041365A
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South Korea
Prior art keywords
endoglucanase
novel
present
endoglucanase gene
gene
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KR1019960042889A
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Korean (ko)
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오두환
허송욱
선보경
이한승
유주현
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오두환
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Priority to KR1019960042889A priority Critical patent/KR960041365A/en
Publication of KR960041365A publication Critical patent/KR960041365A/en

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  • Processing Of Solid Wastes (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

본 발명은 신규한 β-1,4 엔도글루카나제 유전자에 관한 것이다. 좀 더 구체적으로, 본 발명은 토양에서 탐색한 슈도모나스속 YD-15(Pseudomonas sp. YD-15)로부터 분리한 신규한 β-1,4 엔도글루카나제 유전자에 관한 것이다. 본 발명은 토양에서 분리한 슈도모나스속 YD-15와 그로부터 분리한 신규한 β-1,4 엔도글루카나제 유전자의 염기배열 및 그로부터 번역되는 아미노산 서열을 가지는 β-1,4 엔도글루카나제를 제공한다. 본 발명의 β-1,4 엔도글루카나제 유전자 및 그로부터 발현되는 재조합 β-1,4 엔도글루카나제는 섬유소를 알코올 등의 유용성분으로 전환, 종이 폐기물의 분해, 밀로부터 단백질 생산, 과육의 가수분해 등 섬유소를 산업적으로 이용하기 위한 다양한 목적으로 사용되어질 수 있을 것이다.The present invention relates to a novel β-1,4 endoglucanase gene. More specifically, the present invention relates to a novel β-1,4 endoglucanase gene isolated from Pseudomonas sp. YD-15, which is searched for in soil. The present invention provides a β-1,4 endoglucanase having a nucleotide sequence of Pseudomonas genus YD-15 isolated from soil and a novel β-1,4 endoglucanase gene isolated therefrom and an amino acid sequence translated therefrom. do. Β-1,4 endoglucanase gene of the present invention and recombinant β-1,4 endoglucanase expressed therefrom convert fiber to useful components such as alcohol, decompose paper waste, produce protein from wheat, It may be used for various purposes for industrial use of fiber, such as hydrolysis.

Description

신규한 β-1,4 엔도글루카나제 유전자 및 그를 발현하는 미생물Novel β-1,4 Endoglucanase Gene and Microorganisms Expressing It

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제3도는 본 발명의 β-1,4 엔도글루카나제 유전자가 클로닝된 발현벡터를 제조하는 과정을 나타내는 그림이다, 제3도는 본 발명의 β-1,4 엔도글루카나제 유전자를 Bal 31 처리하여 결실 변이체를 제조하는 과정을 나타낸 그림이다, 제5도는 본 발명의 β-1,4 엔도글루카나제 유전자의 염기배열 및 그로부터 번역되는 아미노산서열을 나타낸 그림이다. 제9도는 스트렙토마이세스 레티컬리(Streptomyces reticuli) β-1,4 엔도글루카나제의 아미노산 서열과의 상동성을 나타내는 그림이다.3 is a diagram illustrating a process of preparing an expression vector cloned from the β-1,4 endoglucanase gene of the present invention. FIG. 3 shows Bal 31 treatment of the β-1,4 endoglucanase gene of the present invention. Figure 5 is a diagram showing a process for preparing a deletion variant, Figure 5 is a diagram showing the base sequence of the β-1,4 endoglucanase gene of the present invention and the amino acid sequence translated therefrom. 9 is a diagram showing homology with the amino acid sequence of Streptomyces reticuli β-1,4 endoglucanase.

Claims (4)

β-1,4 엔도글루카나제를 생산하는 슈도모나스속 YD-15(Pseudomonas sp. YD-15) (KFCC-10919).Pseudomonas sp. YD-15 (KFCC-10919), which produces β-1,4 endoglucanase. 제1항의 슈도모나스속 YD-15(Pseudomonas sp. YD-15)로부터 분리한 하기와 같은 염기배열을 가지는 β-1,4 엔도글루카나제의 유전자 :The gene of β-1,4 endoglucanase having the following nucleotide sequence isolated from Pseudomonas sp. YD-15 of claim 1 제2항의 β-1,4 엔도글루카나제 유전자로부터 번역되는 하기와 같은 아미노산 서열을 가지는 β-1,4 엔도글루카나제 :Β-1,4 endoglucanase having the following amino acid sequence translated from the β-1,4 endoglucanase gene of claim 2: 제2항의 유전자가 삽입된 pCHS#3로 형질전환된 Escherichia coli DH5 α(pCHS#3)KFCC-10915.Escherichia coli DH5 α (pCHS # 3) KFCC-10915 transformed with pCHS # 3 having the gene of claim 2 inserted therein. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019960042889A 1996-09-30 1996-09-30 Novel β-1,4 Endoglucanase Gene and Microorganisms Expressing It KR960041365A (en)

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KR1019960042889A KR960041365A (en) 1996-09-30 1996-09-30 Novel β-1,4 Endoglucanase Gene and Microorganisms Expressing It

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Application Number Priority Date Filing Date Title
KR1019960042889A KR960041365A (en) 1996-09-30 1996-09-30 Novel β-1,4 Endoglucanase Gene and Microorganisms Expressing It

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