KR930013111A - Recombinant DNA Compound and Expression Vector Encoding Bacillus para-nitrobenzyl esterase - Google Patents

Recombinant DNA Compound and Expression Vector Encoding Bacillus para-nitrobenzyl esterase Download PDF

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Publication number
KR930013111A
KR930013111A KR1019920024767A KR920024767A KR930013111A KR 930013111 A KR930013111 A KR 930013111A KR 1019920024767 A KR1019920024767 A KR 1019920024767A KR 920024767 A KR920024767 A KR 920024767A KR 930013111 A KR930013111 A KR 930013111A
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South Korea
Prior art keywords
dna
recombinant dna
pnb esterase
promoter
expression
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KR1019920024767A
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Korean (ko)
Inventor
앨리스 칸트웰 캐쓸린
람마 호쥐스 롤란드
맥길브래이 데렉
야트 퀴너 스티븐
리챠드 스와틀링 제임스
마틴 조크 조셉
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원본미기재
일라이 릴리 앤드 캄파니
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Publication of KR930013111A publication Critical patent/KR930013111A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin

Abstract

The present invention provides isolated DNA compounds and recombinant DNA cloning and expression vectors that encode PNB esterase from Bacillus subtilis. The invention also provides host cells transformed with these vectors and a method for production of the PNB esterase by recombinant DNA techniques.

Description

바실러스의 파라-니트로벤질 에스테라제를 암호화하는 재조합 DNA 화합물 및 발현벡터Recombinant DNA Compound and Expression Vector Encoding Bacillus para-nitrobenzyl esterase

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 플라스미드 pNBE1의 제한 효소 부위와 작용지도이다.1 is a functional map of restriction enzyme sites of plasmid pNBE1.

제2도는 플라스미드 pNB106R의 제한 효소 부위와 작용지도이다.2 is a functional map of restriction enzyme sites of plasmid pNB106R.

제3도는 플라스미드 pNB106RM의 제한 효소 부위와 작용지도이다.3 is a functional map of restriction enzyme sites of plasmid pNB106RM.

Claims (13)

바실러스 서브틸리스(Bacillus subtilis)의 PNB에스테라제 활성을 암호화하는 DNA 서열을 포함하는 단리된 DNA 화합물.An isolated DNA compound comprising a DNA sequence encoding PNB esterase activity of Bacillus subtilis. 제1항에 있어서, 명세서에 개시한 바와같은 서열 식별변호:2의 단백질을 암호화하는 단리돤 DNA 화합물.The isolated DNA compound of claim 1, encoding a protein of SEQ ID NO: 2 as disclosed in the specification. 제1항에 있어서, 암호화 가닥이 명세서에 개시한 바와 같은 서열 식별번호:1을 포함하는 단리된 DNA화합물.The isolated DNA compound of claim 1, wherein the coding strand comprises SEQ ID NO: 1 as disclosed in the specification. 제1항 내지 제3항중 어느 한 항에 따른 DNA 서열을 포함하는 제조합 DNA 벡터.A prepared DNA vector comprising a DNA sequence according to any one of claims 1 to 3. 제4항에 있어서, 상기 PNB에스테라제 활성을 암호화하는 DNA의 발현을 유도하기 위해 위치한 프로모터를 또한 포함하는 제조합 DNA 벡터.The recombinant DNA vector of claim 4, further comprising a promoter positioned to induce expression of the DNA encoding the PNB esterase activity. 제5항에 있어서, 상기 프로모터가 에쉐리히아 콜라이(Escherichia coli)에 작용하는 제조합 DNA 벡터.The recombinant DNA vector of claim 5, wherein said promoter acts on Escherichia coli. 제4항 내지 제6항중 어느 한 항에 따른 재조합 DNA 벡터를 사용하여 형질전환시킨 숙주세포.A host cell transformed using the recombinant DNA vector according to any one of claims 4 to 6. 제4항 내지 제6항중 어느 한 항에 따른 재조합 DNA벡터를 사용하여 형질전환시킨 에쉐리히아 콜라이 세포.Escherichia coli cells transformed using the recombinant DNA vector according to any one of claims 4 to 6. (a) PNB 에스테라제 활성을 발현시킬수 있는 재조합 숙주 세포에서 작용하는 프로모터 및 해독 활성화서열 및 (b) 상기 프로모터 및 해독 활성화 서열로 부터의 발현을 위해 위치한 바실러스 서브틸리스의 PNB 에스테라제 활성을 암호화하는 DNA 서열을 포함하는 재조합 DNA 발현 벡터를 사용하여 상기 숙주 세포를 형질전환시킴을 포함하는, 상기 숙주 세포를 제작하는 방법.PNB esterase activity of Bacillus subtilis located for (a) promoter and translational activation sequences acting on recombinant host cells capable of expressing PNB esterase activity and (b) expression from said promoter and translational activation sequences. And transforming said host cell using a recombinant DNA expression vector comprising a DNA sequence encoding DNA. (a)숙주 세포에서 작용하는 프로모터 및 해독 활성화 서열 및 (b) 상기 프로모터 및 해독 활성화 서열로 부터의 발현을 위해 위치한 바실러스 서브틸리스의 PNB 에스테라제 활성을 암호화하는 DNA 서열을 포함하는 재조합 DNA 발현 벡터를 사용하여 형질전환시킨 숙주 세포를 유전자 발현에 적합한 조건하에 배양함을 포함하는, PNB 에스테라제 활성을 발현시키는 방법.a recombinant DNA comprising (a) a promoter and translational activation sequence acting on host cells and (b) a DNA sequence encoding PNB esterase activity of Bacillus subtilis located for expression from said promoter and translational activation sequence A method of expressing PNB esterase activity, comprising culturing a host cell transformed with an expression vector under conditions suitable for gene expression. 제10항에 있어서, 상기 재조합 DNA 발현 벡터가 제2항 또는 제3항에 따른 DNA 서열을 포함하는 방법.The method of claim 10, wherein said recombinant DNA expression vector comprises a DNA sequence according to claim 2. 제11항에 있어서, 상기 숙주 세포가 에쉐리히아 콜라이인 방법.The method of claim 11, wherein said host cell is Escherichia coli. 세팔로스포린 PNB 에스테르 또는 1-카바세팔로스포린 PNB 에스테라제를 제10항, 제11항 또는 제12항의 공정에 의해 제조된 PNB 에스테라제와 반응시킴을 포함하는, 상기 에스테르로부터 p-니트로벤질 그룹을 제거하는 방법.P-nitro from said ester comprising reacting cephalosporin PNB ester or 1-carbacephalosporin PNB esterase with PNB esterase prepared by the process of claims 10, 11 or 12 How to remove benzyl groups. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019920024767A 1991-12-20 1992-12-19 Recombinant DNA Compound and Expression Vector Encoding Bacillus para-nitrobenzyl esterase KR930013111A (en)

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US81109691A 1991-12-20 1991-12-20
US811,096 1991-12-20

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EP (1) EP0551750B1 (en)
JP (1) JPH05276958A (en)
KR (1) KR930013111A (en)
AT (1) ATE152482T1 (en)
BR (1) BR9205055A (en)
CA (1) CA2085806A1 (en)
DE (1) DE69219448T2 (en)
ES (1) ES2101052T3 (en)
HU (1) HUT69769A (en)
IL (1) IL104139A0 (en)
MX (1) MX9207379A (en)
TW (1) TW242161B (en)

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CN1059923C (en) * 1997-02-18 2000-12-27 中国科学院动物研究所 Detoxicating engineering bacteria and its application
DE102005037659A1 (en) * 2005-08-05 2007-02-22 Henkel Kgaa Use of esterases for splitting plastics
US20110268299A1 (en) * 2009-01-05 2011-11-03 Panasonic Corporation Sound field control apparatus and sound field control method
CN105051180B (en) 2013-01-18 2020-06-12 科德克希思公司 Engineered biocatalysts for carbapenem synthesis
JP6399315B2 (en) * 2016-03-18 2018-10-03 株式会社カロテノイド生産技術研究所 Terpene synthase gene, acetoacetate hydrolase gene, and method for producing terpene
CN115927411A (en) * 2022-12-21 2023-04-07 天津大学 Esterase mutant gene, protein expressed by gene and application

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US3972774A (en) * 1975-07-28 1976-08-03 Eli Lilly And Company Enzymatic de-esterification of cephalosporin para-nitrobenzyl esters
MX9207384A (en) * 1991-12-20 1993-07-01 Univ Oklahoma State PURIFIED P-NITROBENCIL ESTEARASE AND PROCEDURE FOR ITS OBTAINING.

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CA2085806A1 (en) 1993-06-20
DE69219448D1 (en) 1997-06-05
HUT69769A (en) 1995-09-28
TW242161B (en) 1995-03-01
ES2101052T3 (en) 1997-07-01
EP0551750A2 (en) 1993-07-21
IL104139A0 (en) 1993-05-13
ATE152482T1 (en) 1997-05-15
MX9207379A (en) 1993-07-01
HU9204037D0 (en) 1993-04-28
EP0551750A3 (en) 1994-08-17
DE69219448T2 (en) 1997-09-04
JPH05276958A (en) 1993-10-26
EP0551750B1 (en) 1997-05-02

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