JPS6447375A - Saccharomyces cerevisiae s6-24 strain - Google Patents

Saccharomyces cerevisiae s6-24 strain

Info

Publication number
JPS6447375A
JPS6447375A JP20179387A JP20179387A JPS6447375A JP S6447375 A JPS6447375 A JP S6447375A JP 20179387 A JP20179387 A JP 20179387A JP 20179387 A JP20179387 A JP 20179387A JP S6447375 A JPS6447375 A JP S6447375A
Authority
JP
Japan
Prior art keywords
yeast
saccharomyces cerevisiae
ynn27
strain
chromorome
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP20179387A
Other languages
Japanese (ja)
Other versions
JPH0351395B2 (en
Inventor
Akira Sakai
Fumio Hishinuma
Yuki Shimizu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP20179387A priority Critical patent/JPS6447375A/en
Publication of JPS6447375A publication Critical patent/JPS6447375A/en
Publication of JPH0351395B2 publication Critical patent/JPH0351395B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PURPOSE:To synthesize Saccharomyces cerevisiae S6-24 strain, by inserting a prescribed recombinant plasmid into yeast Saccharomyces cerevisiae YNN27 chromorome and treating the inserted chromorome with ethylmethane sulfonate. CONSTITUTION:A straight-chain DNA of plasmid pSAK011 is introduced to Saccharomyces cerevisiae YNN27 and the introduced plasmid is inserted into a chromorome of YNN27 to afford a yeast strain of YNN27/pSAK011/S, which is then is cultivated in YPD culture medium, cleaned with a buffer, prepared into a prescribed cell concentration and a prescribed amount of ethylmethane sulfonate which is a mutagenic agent is added to the resultant yeast to treat the yeast and the treated yeast is diluted and cultivated on the YPD culture medium to provide the S6-24 capable of secreting amylase in glucose-containing medium having low concentration (0.2%) in high yield.
JP20179387A 1987-08-14 1987-08-14 Saccharomyces cerevisiae s6-24 strain Granted JPS6447375A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP20179387A JPS6447375A (en) 1987-08-14 1987-08-14 Saccharomyces cerevisiae s6-24 strain

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP20179387A JPS6447375A (en) 1987-08-14 1987-08-14 Saccharomyces cerevisiae s6-24 strain

Publications (2)

Publication Number Publication Date
JPS6447375A true JPS6447375A (en) 1989-02-21
JPH0351395B2 JPH0351395B2 (en) 1991-08-06

Family

ID=16447026

Family Applications (1)

Application Number Title Priority Date Filing Date
JP20179387A Granted JPS6447375A (en) 1987-08-14 1987-08-14 Saccharomyces cerevisiae s6-24 strain

Country Status (1)

Country Link
JP (1) JPS6447375A (en)

Also Published As

Publication number Publication date
JPH0351395B2 (en) 1991-08-06

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Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term