RU2001104431A - METHOD FOR PRODUCING PROTEINS IN TRANSFORMED YEAST CELLS - Google Patents

METHOD FOR PRODUCING PROTEINS IN TRANSFORMED YEAST CELLS

Info

Publication number
RU2001104431A
RU2001104431A RU2001104431/13A RU2001104431A RU2001104431A RU 2001104431 A RU2001104431 A RU 2001104431A RU 2001104431/13 A RU2001104431/13 A RU 2001104431/13A RU 2001104431 A RU2001104431 A RU 2001104431A RU 2001104431 A RU2001104431 A RU 2001104431A
Authority
RU
Russia
Prior art keywords
antibiotic resistance
gene
yeast
marker gene
strain
Prior art date
Application number
RU2001104431/13A
Other languages
Russian (ru)
Other versions
RU2238323C2 (en
Inventor
Томас КЬЕЛЛЬСЕН
Кнуд Вад
Original Assignee
Ново Нордиск А/С
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ново Нордиск А/С filed Critical Ново Нордиск А/С
Publication of RU2001104431A publication Critical patent/RU2001104431A/en
Application granted granted Critical
Publication of RU2238323C2 publication Critical patent/RU2238323C2/en

Links

Claims (6)

1. Рекомбинантный дрожжевой экспрессирующий вектор, неспособный сообщать устойчивость к антибиотикам бактериальным клеткам, причем указанный вектор содержит ген, кодирующий гетерологичный ген и ген-маркер устойчивости к антибиотикам, который сделан нефункциональным в результате модификации in vitro.1. A recombinant yeast expression vector incapable of reporting antibiotic resistance to bacterial cells, said vector containing a gene encoding a heterologous gene and a marker gene for antibiotic resistance, which is rendered non-functional by in vitro modification. 2. Рекомбинантный дрожжевой экспрессирующий вектор по п. 1, в котором ген-маркер устойчивости к антибиотикам является геном АМР. 2. The recombinant yeast expression vector according to claim 1, wherein the antibiotic resistance marker gene is an AMP gene. 3. Способ получения целевого полипептида или белка, включающий культивирование дрожжевого штамма, содержащего вектор по пп. 1 и 2, в приемлемых условиях и выделение целевого продукта из культуральной среды. 3. A method of obtaining the target polypeptide or protein, comprising cultivating a yeast strain containing the vector according to claims. 1 and 2, under acceptable conditions and the selection of the target product from the culture medium. 4. Способ получения целевого полипептида или белка, включающий культивирование в приемлемых условиях дрожжевого штамма, содержащего дрожжевую экспрессирующую плазмиду, причем в указанной плазмиде функциональный ген-маркер устойчивости к антибиотикам, используемый на начальных стадиях клонирования в бактериях, сделан нефункциональным в результате делеции in vitro части или всего гена-маркера до введения в дрожжевую клетку-хозяина. 4. A method of obtaining the target polypeptide or protein, comprising cultivating, under acceptable conditions, a yeast strain containing a yeast expressing plasmid, wherein the functional antibiotic resistance marker marker used in the initial stages of cloning in bacteria is not functional as a result of an in vitro deletion or the entire marker gene prior to introduction into the yeast host cell. 5. Способ по п. 4, включающий введение приемлемых сайтов рестрикции с обеих сторон от гена-маркера устойчивости к антибиотикам и удаление гена-маркера в результате обработки in vitro приемлемыми рестрикционными ферментами. 5. The method of claim 4, comprising introducing acceptable restriction sites on both sides of the antibiotic resistance marker gene and removing the marker gene as a result of in vitro treatment with acceptable restriction enzymes. 6. Способ по пп. 3-5, в котором дрожжевой штамм является штаммом Saccharomyces, предпочтительно штаммом Saccharomyces cerevisiae. 6. The method according to PP. 3-5, in which the yeast strain is a strain of Saccharomyces, preferably a strain of Saccharomyces cerevisiae.
RU2001104431A 1998-07-16 1999-07-02 Method for preparing proteins in transformed yeast cells RU2238323C2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA199800945 1998-07-16
DKPA199800945 1998-07-16
DKPA199900754 1999-05-28
DKPA199900754 1999-05-28

Publications (2)

Publication Number Publication Date
RU2001104431A true RU2001104431A (en) 2003-03-10
RU2238323C2 RU2238323C2 (en) 2004-10-20

Family

ID=26064588

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2001104431A RU2238323C2 (en) 1998-07-16 1999-07-02 Method for preparing proteins in transformed yeast cells

Country Status (16)

Country Link
EP (1) EP1097228B1 (en)
JP (1) JP4668414B2 (en)
KR (1) KR20010071929A (en)
CN (1) CN1187451C (en)
AT (1) ATE318919T1 (en)
AU (1) AU4499399A (en)
BR (1) BR9912106B1 (en)
CA (1) CA2337635A1 (en)
DE (1) DE69930118T2 (en)
ES (1) ES2260917T3 (en)
HU (1) HUP0102697A3 (en)
IL (1) IL140403A0 (en)
NO (1) NO20010246D0 (en)
PL (1) PL201350B1 (en)
RU (1) RU2238323C2 (en)
WO (1) WO2000004172A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100783287B1 (en) * 2006-08-09 2007-12-06 한국생명공학연구원 Construction of a strain for identification of compounds relieving transcriptional silencing at telomere
CN105308067B (en) 2013-06-07 2020-07-24 诺和诺德股份有限公司 Method for preparing mature insulin polypeptide
US20220002738A1 (en) * 2018-11-19 2022-01-06 Jingping Zhong Recombinant yeast cell

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1321523A3 (en) * 1993-07-23 2004-03-03 DSM IP Assets B.V. Selection marker gene free recombinant strains; a method for obtaining them and the use of these strains
DE4428402A1 (en) * 1994-08-11 1996-02-15 Boehringer Mannheim Gmbh Gene therapy method using DNA vectors without a selection marker gene
JP4090090B2 (en) * 1996-06-21 2008-05-28 サントリー株式会社 Method for producing transformant having no selectable marker gene
EP0814165B1 (en) * 1996-06-21 2003-04-09 Suntory Limited Method for constructing transformed yeast cells, that do not contain a selective marker gene

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