ES2173812B1 - Dermis artificial y metodo de obtencion. - Google Patents
Dermis artificial y metodo de obtencion.Info
- Publication number
- ES2173812B1 ES2173812B1 ES200100494A ES200100494A ES2173812B1 ES 2173812 B1 ES2173812 B1 ES 2173812B1 ES 200100494 A ES200100494 A ES 200100494A ES 200100494 A ES200100494 A ES 200100494A ES 2173812 B1 ES2173812 B1 ES 2173812B1
- Authority
- ES
- Spain
- Prior art keywords
- artificial
- skin
- artificial dermis
- dermis
- plasma
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/10—Hair or skin implants
- A61F2/105—Skin implants, e.g. artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/33—Fibroblasts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
Abstract
Dermis artificial y método de obtención. Dermis artificial (1) obtenida a partir de plasma con plaquetas (2) y fibroblastos (3) humanos. El plasma con plaquetas (2) se obtiene por fraccionamiento de la sangre total (4) del paciente (8) mediante centrifugación ligera, y los fibroblastos (3) humanos a partir de una biopsia de piel (5). La gelificación se consigue mediante la adición de calcio. Esta dermis artificial (1) permite un crecimiento rápido de los queratinocitos (6) sembrados en su superficie para constituir una piel artificial (7) que es fácilmente trasplantable. Se consiguen grandes superficies de dermis artificial (1) a partir de una pequeña biopsia de piel (5) y de cantidades mínimas de plasma con plaquetas (2), que al estar enriquecido en citoquinas y factores de crecimiento plaquetario potencia la proliferación de las células sembradas, tanto en su interior como en su superficie. La piel artificial (7) así obtenida puede servir para el tratamiento de grandes quemados, úlceras cutáneas crónicas... o utilizarse, mediante el empleo de células genéticamente modificadas, como base para terapia génica.
Priority Applications (20)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200100494A ES2173812B1 (es) | 2001-03-01 | 2001-03-01 | Dermis artificial y metodo de obtencion. |
TW091103519A TWI258506B (en) | 2001-03-01 | 2002-02-27 | Artificial dermis and method of preparation |
EP09014041A EP2165678A1 (en) | 2001-03-01 | 2002-02-28 | Artificial dermis and production method therefor |
PCT/ES2002/000087 WO2002072800A1 (es) | 2001-03-01 | 2002-02-28 | Dermis artificial y metodo de obtencion |
ARP020100722A AR032916A1 (es) | 2001-03-01 | 2002-02-28 | Dermis artificial y metodo de obtencion. |
ES02702424T ES2336430T3 (es) | 2001-03-01 | 2002-02-28 | Dermis artificial y metodo de obtecion. |
AU2002235958A AU2002235958B2 (en) | 2001-03-01 | 2002-02-28 | Artificial dermis and production method therefor |
US10/469,554 US7244552B2 (en) | 2001-03-01 | 2002-02-28 | Artificial dermis and production method therefor |
AT02702424T ATE448295T1 (de) | 2001-03-01 | 2002-02-28 | Künstliche dermis und herstellungsverfahren dafür |
DE60234323T DE60234323D1 (de) | 2001-03-01 | 2002-02-28 | Künstliche dermis und herstellungsverfahren dafür |
PT02702424T PT1375647E (pt) | 2001-03-01 | 2002-02-28 | Derme artificial e respectivo método de produção |
CA2439387A CA2439387C (en) | 2001-03-01 | 2002-02-28 | Artificial dermis and production method therefor |
DK02702424.9T DK1375647T3 (da) | 2001-03-01 | 2002-02-28 | Kunstig dermis og fremstillingsfremgangsmåde dertil |
JP2002571856A JP4235452B2 (ja) | 2001-03-01 | 2002-02-28 | 人工真皮及びその製造方法 |
EP02702424A EP1375647B1 (en) | 2001-03-01 | 2002-02-28 | Artificial dermis and production method therefor |
EG20020229A EG23321A (en) | 2001-03-01 | 2002-03-02 | Artificial dermis and method of preparation. |
US11/809,665 US20070275461A1 (en) | 2001-03-01 | 2007-05-31 | Artificial dermis and method of preparation |
JP2008118300A JP5047047B2 (ja) | 2001-03-01 | 2008-04-30 | 人工真皮 |
US13/367,548 US9168125B2 (en) | 2001-03-01 | 2012-02-07 | Artificial dermis and method of preparation |
JP2012107526A JP5529918B2 (ja) | 2001-03-01 | 2012-05-09 | 人工真皮 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
ES200100494A ES2173812B1 (es) | 2001-03-01 | 2001-03-01 | Dermis artificial y metodo de obtencion. |
Publications (2)
Publication Number | Publication Date |
---|---|
ES2173812A1 ES2173812A1 (es) | 2002-10-16 |
ES2173812B1 true ES2173812B1 (es) | 2003-12-16 |
Family
ID=8496947
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES200100494A Expired - Fee Related ES2173812B1 (es) | 2001-03-01 | 2001-03-01 | Dermis artificial y metodo de obtencion. |
ES02702424T Expired - Lifetime ES2336430T3 (es) | 2001-03-01 | 2002-02-28 | Dermis artificial y metodo de obtecion. |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES02702424T Expired - Lifetime ES2336430T3 (es) | 2001-03-01 | 2002-02-28 | Dermis artificial y metodo de obtecion. |
Country Status (14)
Country | Link |
---|---|
US (3) | US7244552B2 (es) |
EP (2) | EP2165678A1 (es) |
JP (3) | JP4235452B2 (es) |
AR (1) | AR032916A1 (es) |
AT (1) | ATE448295T1 (es) |
AU (1) | AU2002235958B2 (es) |
CA (1) | CA2439387C (es) |
DE (1) | DE60234323D1 (es) |
DK (1) | DK1375647T3 (es) |
EG (1) | EG23321A (es) |
ES (2) | ES2173812B1 (es) |
PT (1) | PT1375647E (es) |
TW (1) | TWI258506B (es) |
WO (1) | WO2002072800A1 (es) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7144729B2 (en) | 2000-09-01 | 2006-12-05 | Dfb Pharmaceuticals, Inc. | Methods and compositions for tissue regeneration |
ES2173812B1 (es) * | 2001-03-01 | 2003-12-16 | Ct Investig Energeticas Ciemat | Dermis artificial y metodo de obtencion. |
JP2004075661A (ja) * | 2002-06-18 | 2004-03-11 | Shiseido Co Ltd | 表皮基底膜ケアを特徴とする皮膚外用剤、皮膚基底膜構造形成促進剤および人工皮膚の製造方法 |
GB0224986D0 (en) | 2002-10-28 | 2002-12-04 | Smith & Nephew | Apparatus |
US11298453B2 (en) | 2003-10-28 | 2022-04-12 | Smith & Nephew Plc | Apparatus and method for wound cleansing with actives |
EP1718317A1 (en) * | 2004-02-13 | 2006-11-08 | Intercytex Limited | Wound healing profile |
JP2007530016A (ja) * | 2004-02-27 | 2007-11-01 | ザ ジェネラル ホスピタル コーポレーション | 発毛のための方法および組成物 |
US7753894B2 (en) | 2004-04-27 | 2010-07-13 | Smith & Nephew Plc | Wound cleansing apparatus with stress |
GB0505202D0 (en) | 2005-03-14 | 2005-04-20 | Intercytex Ltd | Skin equivalent culture |
ES2263382B1 (es) * | 2005-05-16 | 2007-11-16 | Fundacion Para La Investigacion Biomedica Del Hospital Gregorio Marañon | Matriz artificial de gel de fibrina endotelizada superproductora de factores proangiogenicos. |
EP1885280A1 (en) | 2005-05-26 | 2008-02-13 | Intercytex Limited | Tissue repair using allogenic dermal fibroblasts |
EP1991051A1 (en) * | 2006-03-03 | 2008-11-19 | Ciemat | A mouse model comprising an engrafted human skin having hypersensitivity to uv-light |
US8709081B2 (en) | 2006-10-23 | 2014-04-29 | Stemedica Cell Technologies, Inc. | Cellular scaffold |
US8105380B2 (en) * | 2006-10-23 | 2012-01-31 | Stemedica Cell Technologies, Inc. | Cellular scaffold |
US20100303770A1 (en) * | 2006-12-28 | 2010-12-02 | John Maslowski | Methods for culturing dermal cells for treatment of skin injuries such as burns |
JP5253749B2 (ja) * | 2007-03-19 | 2013-07-31 | 肇 井上 | 表皮シートの作製方法および初代培養時における培養容器への上皮幹細胞の接着性付与方法 |
WO2009051701A2 (en) * | 2007-10-15 | 2009-04-23 | Wake Forest University Health Sciences | Methods and compositions for printing biologically compatible nanotube composites of autologous tissue |
CA2716752A1 (en) * | 2008-02-29 | 2009-09-03 | Showa University | Method for producing artificial skin |
JP6134933B2 (ja) | 2009-01-27 | 2017-05-31 | レッドドレス リミテッド | 創傷包帯剤、これを作製するための方法及び器具及びその保存及び使用 |
TR201809713T4 (tr) * | 2009-08-25 | 2018-07-23 | Servicio Andaluz De Salud | Agaroz-fibrin biyomalzemelerini kullanarak doku mühendisliğiyle yapay doku üretimi. |
JP5737860B2 (ja) * | 2010-04-30 | 2015-06-17 | テルモ株式会社 | ゲル状細胞組成物およびその製造方法 |
CL2011001870A1 (es) * | 2011-08-03 | 2012-01-27 | U Tecnica Federico Santa Maria 28 71% | Proceso de preparacion de un gel de fibrina autologo o desde sangre compatible para proliferacion y vehiculizacion celular; gel de fibrina o de sangre compatible; uso del gel de fibrina o de sangre compatible para uso quirurgico. |
TWI448310B (zh) * | 2012-05-23 | 2014-08-11 | Medical dressings and their coating methods for frozen freeze - dried platelets | |
TWI448312B (zh) * | 2012-05-23 | 2014-08-11 | An implant of freeze - dried platelets and its coating method | |
GB201303485D0 (en) | 2013-02-27 | 2013-04-10 | Alcyomics Ltd | Skin model |
MX342233B (es) * | 2014-02-04 | 2016-08-24 | Alvaro Galue Eduardo | Proceso de obtencion de un compuesto de aspersion celular de fibroblastos y queratinocitos humanos en solucion y su uso como agente regenerativo de lesiones cutáneas. |
ES2551143B1 (es) * | 2014-05-13 | 2016-07-01 | Fundación Tekniker | Dermis artificial, piel artificial, métodos para su preparación y sus usos |
CN105505855A (zh) * | 2016-02-04 | 2016-04-20 | 关志广 | 一种具备增殖能力的自体表皮细胞apg凝胶的制作方法 |
DK3463498T3 (da) * | 2016-05-31 | 2021-12-20 | Octapharma Ag | Plasmabaserede film og fremgangsmåder til fremstilling og anvendelse af den samme. |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4485096A (en) * | 1982-02-26 | 1984-11-27 | Massachusetts Institute Of Technology | Tissue-equivalent and method for preparation thereof |
JPS6379588A (ja) * | 1986-09-25 | 1988-04-09 | Yasuo Moriya | 細胞培養用基材およびその製造方法 |
DK475386D0 (da) * | 1986-10-03 | 1986-10-03 | Weis Fogh Ulla Sivertsen | Fremgangsmaade og apparat til fremstilling af biologiske stoffer |
FR2639958B1 (fr) | 1988-12-06 | 1992-08-21 | Lille Transfusion Sanguine | Support biologique pour cultures cellulaires constitue de proteines plasmatiques coagulees par la thrombine, son utilisation pour la culture des keratinocytes, leur recuperation et leur transport a des fins d'utilisation therapeutique |
RU2126259C1 (ru) * | 1992-05-29 | 1999-02-20 | Дзе Юниверсити оф Нос Каролина эт Чепел Хилл | Фармацевтически приемлемые фиксированные высушенные тромбоциты крови человека |
JP3091113B2 (ja) * | 1994-11-22 | 2000-09-25 | 科学技術振興事業団 | 生理活性物質分泌性のハイブリッド型ゲル |
US5733545A (en) * | 1995-03-03 | 1998-03-31 | Quantic Biomedical Partners | Platelet glue wound sealant |
WO1997001533A1 (fr) | 1995-06-27 | 1997-01-16 | The Green Cross Corporation | Derives d'acide thiocarbamique |
WO2000062828A1 (en) * | 1996-04-30 | 2000-10-26 | Medtronic, Inc. | Autologous fibrin sealant and method for making the same |
ES2117573B1 (es) * | 1996-07-19 | 1999-02-16 | Comunitario De Transfusion Del | Base dermica especialmente diseñada para el cultivo de queratinocitos. |
JP3333108B2 (ja) * | 1997-04-08 | 2002-10-07 | 学校法人東海大学 | 上皮性組織移植片およびその製造方法 |
ES2132027B1 (es) * | 1997-07-04 | 2000-04-01 | Comunitario De Transfusion Del | Desarrollo de una piel artificial mediante cultivo de queratinocitos sobre una base de fibrina y fibroblastos humanos y metodo de preparacion de esta piel para trasplante. |
JPH11239609A (ja) * | 1998-02-26 | 1999-09-07 | Sekisui Chem Co Ltd | 血小板含有生体組織用接着剤 |
JPH11246420A (ja) * | 1998-02-26 | 1999-09-14 | Sekisui Chem Co Ltd | 創傷治癒促進剤 |
ES2173812B1 (es) * | 2001-03-01 | 2003-12-16 | Ct Investig Energeticas Ciemat | Dermis artificial y metodo de obtencion. |
ES2184623B1 (es) * | 2001-06-29 | 2004-09-16 | Centro De Investigaciones Energeticas, Medioambientales Y Tecnologicas (C.I.E.M.A.T.) | Piel artificial autologa secretora de leptina y metodo de obtencion. |
US20030166274A1 (en) * | 2001-11-15 | 2003-09-04 | Hewitt Charles W. | Three-dimensional matrix for producing living tissue equivalents |
US20040126881A1 (en) * | 2002-09-06 | 2004-07-01 | Vincent Ronfard | Fibrin cell supports and methods of use thereof |
-
2001
- 2001-03-01 ES ES200100494A patent/ES2173812B1/es not_active Expired - Fee Related
-
2002
- 2002-02-27 TW TW091103519A patent/TWI258506B/zh not_active IP Right Cessation
- 2002-02-28 AU AU2002235958A patent/AU2002235958B2/en not_active Ceased
- 2002-02-28 EP EP09014041A patent/EP2165678A1/en not_active Withdrawn
- 2002-02-28 CA CA2439387A patent/CA2439387C/en not_active Expired - Fee Related
- 2002-02-28 EP EP02702424A patent/EP1375647B1/en not_active Expired - Lifetime
- 2002-02-28 ES ES02702424T patent/ES2336430T3/es not_active Expired - Lifetime
- 2002-02-28 WO PCT/ES2002/000087 patent/WO2002072800A1/es active IP Right Grant
- 2002-02-28 US US10/469,554 patent/US7244552B2/en not_active Expired - Fee Related
- 2002-02-28 AR ARP020100722A patent/AR032916A1/es not_active Application Discontinuation
- 2002-02-28 DK DK02702424.9T patent/DK1375647T3/da active
- 2002-02-28 DE DE60234323T patent/DE60234323D1/de not_active Expired - Lifetime
- 2002-02-28 PT PT02702424T patent/PT1375647E/pt unknown
- 2002-02-28 AT AT02702424T patent/ATE448295T1/de active
- 2002-02-28 JP JP2002571856A patent/JP4235452B2/ja not_active Expired - Fee Related
- 2002-03-02 EG EG20020229A patent/EG23321A/xx active
-
2007
- 2007-05-31 US US11/809,665 patent/US20070275461A1/en not_active Abandoned
-
2008
- 2008-04-30 JP JP2008118300A patent/JP5047047B2/ja not_active Expired - Fee Related
-
2012
- 2012-02-07 US US13/367,548 patent/US9168125B2/en not_active Expired - Fee Related
- 2012-05-09 JP JP2012107526A patent/JP5529918B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
ATE448295T1 (de) | 2009-11-15 |
AU2002235958B2 (en) | 2007-05-10 |
ES2173812A1 (es) | 2002-10-16 |
US20040171145A1 (en) | 2004-09-02 |
EP2165678A1 (en) | 2010-03-24 |
JP2004522545A (ja) | 2004-07-29 |
EP1375647B1 (en) | 2009-11-11 |
US7244552B2 (en) | 2007-07-17 |
EG23321A (en) | 2004-12-29 |
JP2012187412A (ja) | 2012-10-04 |
CA2439387A1 (en) | 2002-09-19 |
DK1375647T3 (da) | 2010-03-15 |
TWI258506B (en) | 2006-07-21 |
CA2439387C (en) | 2012-04-24 |
DE60234323D1 (de) | 2009-12-24 |
US20120183505A1 (en) | 2012-07-19 |
JP4235452B2 (ja) | 2009-03-11 |
JP5047047B2 (ja) | 2012-10-10 |
JP2008253788A (ja) | 2008-10-23 |
US9168125B2 (en) | 2015-10-27 |
JP5529918B2 (ja) | 2014-06-25 |
WO2002072800A1 (es) | 2002-09-19 |
EP1375647A1 (en) | 2004-01-02 |
US20070275461A1 (en) | 2007-11-29 |
PT1375647E (pt) | 2010-02-18 |
ES2336430T3 (es) | 2010-04-13 |
AR032916A1 (es) | 2003-12-03 |
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