ES2157230T3 - Deteccion de mycobacteria por amplificacion multiple de acidos nucleicos. - Google Patents

Deteccion de mycobacteria por amplificacion multiple de acidos nucleicos.

Info

Publication number
ES2157230T3
ES2157230T3 ES94112741T ES94112741T ES2157230T3 ES 2157230 T3 ES2157230 T3 ES 2157230T3 ES 94112741 T ES94112741 T ES 94112741T ES 94112741 T ES94112741 T ES 94112741T ES 2157230 T3 ES2157230 T3 ES 2157230T3
Authority
ES
Spain
Prior art keywords
amplification
objectives
sequences
sample
species
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94112741T
Other languages
English (en)
Inventor
George Terrance Walker
James G Nadeau
Patricia Anne Spears
Colleen M Nycz
Daryl Dee Shank
James L Schram
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2157230T3 publication Critical patent/ES2157230T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

IMPRIMADORES Y METODOS PARA LA AMPLIFICACION MULTIPLES MEDIADA POR UN ADAPTADOR DEL ELEMENTO DE INSERCION IS6110 DE LA MYCOBACTERIUM TUBERCULOSIS (M. TB) Y DEL GEN RIBOSOMAL 16S DE LA MYCOBACTERIUM TUBERCULOSIS, UTIL PARA DETECTAR Y/O IDENTIFICAR SIMULTANEAMENTE ESPECIES DEL COMPLEJO M. TUBERCULOSIS Y OTRAS ESPECIES DE MYCOBACTERIUM CLINICAMENTE RELEVANTES. LA AMPLIFICACION MULTIPLEX POR DESPLAZAMIENTO DE LAS ESPECIES (SDA) SE UTILIZA EN UNA SOLA REACCION DE AMPLIFICACION QUE ES CAPAZ DE IDENTIFICAR UNA M. TUBERCULOSIS Y SIMULTANEAMENTE LLEVAR A CABO UNA DETECCION DE SUSTANCIALMENTE TODAS LAS ESPECIES CLINICAMENTE RELEVANTES DE MYCOBACTERIA. TAMBIEN SE PRESENTAN LOS METODOS PARA LLEVAR A CABO UNA AMPLIFICACION MULTIPLEX MEDIADA POR UN ADAPTADOR EN MULTIPLES SECUENCIAS OBJETIVO Y UNA SOLA SECUENCIA DE CONTROL INTERNA PARA DETERMINAR LA EFICACIA DE UNA MUESTRA Y LA CANTIDAD DE OBJETIVOS. EN UN ASPECTO PREFERENTE DE LA INVENCION, SE INCLUYE UNA SECUENCIA DE CONTROL INTERNA EN LA REACCION DE AMPLIFICACION Y SE COAMPLIFICA CON LAS SECUENCIAS IS6110 Y 16S OBJETIVO PARA INDICAR LA ACTIVIDAD DE AMPLIFICACION DE LAS MUESTRA Y PARA CUANTIFICAR LA CANTIDAD DE SECUENCIAS OBJETIVO QUE SE ENCUENTRAN EN LA MUESTRA.
ES94112741T 1993-08-24 1994-08-16 Deteccion de mycobacteria por amplificacion multiple de acidos nucleicos. Expired - Lifetime ES2157230T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/111,076 US5470723A (en) 1993-05-05 1993-08-24 Detection of mycobacteria by multiplex nucleic acid amplification

Publications (1)

Publication Number Publication Date
ES2157230T3 true ES2157230T3 (es) 2001-08-16

Family

ID=22336475

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94112741T Expired - Lifetime ES2157230T3 (es) 1993-08-24 1994-08-16 Deteccion de mycobacteria por amplificacion multiple de acidos nucleicos.

Country Status (9)

Country Link
US (3) US5470723A (es)
EP (1) EP0640691B1 (es)
JP (1) JP2814422B2 (es)
AU (1) AU686169B2 (es)
BR (1) BR9403324A (es)
CA (1) CA2129690A1 (es)
DE (1) DE69426956T2 (es)
ES (1) ES2157230T3 (es)
SG (1) SG44897A1 (es)

Families Citing this family (116)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712095A (en) * 1994-06-16 1998-01-27 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
US6001610A (en) * 1994-11-23 1999-12-14 Roche Diagnostics, Gmbh Method for the particularly sensitive detection of nucleic acids
CA2163393C (en) * 1994-11-30 2003-04-22 Colleen Marie Nycz Amplification and detection of mycobacteria nucleic acids
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
DE69633941T2 (de) * 1995-12-22 2005-11-24 Dade Behring Marburg Gmbh Nachweis von unterschieden in nukleinsäuren
US5691143A (en) * 1996-03-15 1997-11-25 Becton, Dickinson And Company Amplification and detection of the alpha antigen gene of mycobacterium avium complex species
AU723678B2 (en) * 1996-03-18 2000-08-31 Molecular Biology Resources, Inc. Target nucleic acid sequence amplification
US5811269A (en) * 1996-04-30 1998-09-22 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5858665A (en) * 1996-07-25 1999-01-12 Navix, Inc. Homogeneous diagnostic assay method utilizing simultaneous target and signal amplification
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
WO1999019510A1 (en) * 1997-10-10 1999-04-22 President And Fellows Of Harvard College Surface-bound, double-stranded dna protein arrays
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US7090975B2 (en) * 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
AU3984699A (en) * 1998-05-12 1999-11-29 Whitehead Institute For Biomedical Research Multiplex dna amplification using chimeric primers
US8445664B2 (en) * 1998-06-24 2013-05-21 Enzo Diagnostics, Inc. Kits for amplifying and detecting nucleic acid sequences
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
JP2002525078A (ja) * 1998-09-15 2002-08-13 イェール ユニバーシティ 人工的長末端反復配列
WO2000062036A1 (en) * 1999-04-12 2000-10-19 Nanogen/Becton Dickinson Partnership Amplification and separation of nucleic acid sequences using strand displacement amplification and bioelectronic microchip technology
US6326173B1 (en) * 1999-04-12 2001-12-04 Nanogen/Becton Dickinson Partnership Electronically mediated nucleic acid amplification in NASBA
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US6531302B1 (en) 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
US6309833B1 (en) 1999-04-12 2001-10-30 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences on a bioelectronic microchip using asymmetric structures
US7595383B1 (en) * 1999-05-04 2009-09-29 University Of Medicine And Dentistry Of New Jersey Secreted proteins of Mycobacterium tuberculosis and their use as vaccines and diagnostic reagents
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US6664081B2 (en) * 1999-12-17 2003-12-16 Gen-Probe Incorporated Nucleic acid amplification and detection of mycobacterium species
AU2001241934A1 (en) * 2000-03-03 2001-09-17 Beckman Coulter Inc. Multiplex hybridization system for identification of pathogenic mycobacterium and method of use
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
EP2351853A1 (en) 2000-06-06 2011-08-03 Life Technologies Corporation Method and devices for multiplexing amplification reactions
US7087414B2 (en) * 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
AU7104200A (en) 2000-09-01 2002-03-22 Gen Probe Inc Amplification of hiv-1 sequences for detection of sequences associated with drug-resistance mutations
US6844152B1 (en) * 2000-09-15 2005-01-18 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US20020058265A1 (en) * 2000-09-15 2002-05-16 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US6492120B1 (en) 2000-11-02 2002-12-10 University Of Maryland Nucleic acid hybridization assay utilizing tricyclic target and signal amplification
DE10061166A1 (de) * 2000-11-30 2002-06-06 Attomol Gmbh Molekulare Diagno Verfahren und Kit zum direkten Nachweis von Nukleotidsequenzen, Aminosäuresequenzen oder Antigenen
WO2002052043A1 (fr) * 2000-12-26 2002-07-04 Takara Bio Inc. Procede de detection d'un micro-organisme pathogene
WO2002070751A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
US20020165175A1 (en) * 2001-04-17 2002-11-07 Xiaowu Liang Fast and enzymeless cloning of nucleic acid fragments
US20030104421A1 (en) * 2001-05-07 2003-06-05 Colangelo Christopher M. Methods and compositions for nucleic acid amplification
US20030165911A1 (en) * 2001-07-15 2003-09-04 Keck Graduate Institute Gene expression analysis using nicking agents
WO2003008624A2 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Nucleic acid amplification using nicking agents
CA2492007A1 (en) * 2001-07-15 2003-01-30 Keck Graduate Institute Amplification of nucleic acid fragments using nicking agents
KR100455032B1 (ko) * 2002-03-18 2004-11-06 바이오코아 주식회사 다중-이중 중합효소연쇄반응에 의한 결핵균과 비결핵균의동시 검출 방법
DE10213537A1 (de) * 2002-03-26 2003-10-30 Hain Lifescience Gmbh Verfahren und Differenzierung des Mycobacterium tuberculosis-Komplex
US20030235827A1 (en) * 2002-06-25 2003-12-25 Orchid Biosciences, Inc. Methods and compositions for monitoring primer extension and polymorphism detection reactions
AU2003247715B8 (en) * 2002-06-28 2008-06-05 Orchid Cellmark Inc. Methods and compositions for analyzing compromised samples using single nucleotide polymorphism panels
JP2006517385A (ja) * 2002-08-02 2006-07-27 オーキッド セルマーク インコーポレイテッド 遺伝子型特定のための方法および組成物
US7192700B2 (en) * 2002-12-20 2007-03-20 Orchid Cellmark Inc. Methods and compositions for conducting primer extension and polymorphism detection reactions
US20060073475A1 (en) * 2002-08-09 2006-04-06 Nanibhushan Dattagupta Compositions and methods for detecting pathogenic bacteria expressing chaperonin proteins
US7662594B2 (en) * 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
DE60324810D1 (de) * 2002-09-20 2009-01-02 New England Biolabs Inc HELICASE-ABHuNGIGE AMPLIFIKATION VON NUKLEINSUREN
JP2006508662A (ja) 2002-12-04 2006-03-16 アプレラ コーポレイション ポリヌクレオチドの多重増幅
US20040115643A1 (en) * 2002-12-12 2004-06-17 Lizardi Paul M. Thermodynamic equilibrium extension of primers
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20040259100A1 (en) * 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US20050181394A1 (en) * 2003-06-20 2005-08-18 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
US20050158710A1 (en) * 2004-01-16 2005-07-21 Shirley Tsang Detection of enterovirus nucleic acid
US7481997B1 (en) 2004-02-12 2009-01-27 Montana State University Snow mountain virus genome sequence, virus-like particles and methods of use
US7229800B2 (en) * 2004-04-16 2007-06-12 Becton, Dickinson And Company Neisseria gonorrhoeae assay
WO2005103249A1 (ja) 2004-04-26 2005-11-03 Wako Pure Chemical Industries, Ltd. 結核菌検出用プライマー及びプローブ、並びにこれを用いたヒト型結核菌の検出方法
WO2006017724A1 (en) * 2004-08-06 2006-02-16 Becton, Dickinson And Company Sequences and methods for detection of cytomegalovirus
US20060040258A1 (en) * 2004-08-23 2006-02-23 Huiyan Guo Water-soluble conjugates and methods of preparation
KR20070085275A (ko) * 2004-09-20 2007-08-27 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 멀티플 모드에 의한 멀티플렉스 반응 억제 방법
US20060073511A1 (en) 2004-10-05 2006-04-06 Affymetrix, Inc. Methods for amplifying and analyzing nucleic acids
US8158388B2 (en) * 2004-10-21 2012-04-17 New England Biolabs, Inc. Repair of nucleic acids for improved amplification
KR20070073917A (ko) * 2004-10-21 2007-07-10 뉴 잉글랜드 바이오랩스, 인크 개선된 증폭을 위한 핵산 복구
US20060205090A1 (en) * 2005-03-14 2006-09-14 Newton Michael W Water-soluble conjugates for electrochemical detection
EP1863908B1 (de) 2005-04-01 2010-11-17 Qiagen GmbH Reverse transkription und amplifikation von rna bei simultaner degradierung von dna
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
JP5654749B2 (ja) * 2006-04-11 2015-01-14 ニユー・イングランド・バイオレイブス・インコーポレイテツド 改良された増幅のための核酸の修復
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US8198027B2 (en) 2006-12-21 2012-06-12 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US20090215050A1 (en) * 2008-02-22 2009-08-27 Robert Delmar Jenison Systems and methods for point-of-care amplification and detection of polynucleotides
US20090227463A1 (en) * 2008-03-04 2009-09-10 Reif John H Autonomous in vitro evolution
US9416426B2 (en) 2008-10-27 2016-08-16 Becton, Dickinson And Company Assay for Chlamydia trachomatis by amplification and detection of Chlamydia trachomatis pmpA gene
US8945842B2 (en) 2009-01-14 2015-02-03 Becton, Dickinson And Company Assay for Trichomonas vaginalis by amplification and detection of Trichomonas vaginalis AP65-1 gene
WO2010091111A1 (en) 2009-02-03 2010-08-12 Biohelix Corporation Endonuclease-enhanced helicase-dependent amplification
WO2011003020A1 (en) 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
WO2011047223A1 (en) 2009-10-16 2011-04-21 Monsanto Technology Llc Methods of polynucleotide detection
US9121054B2 (en) * 2009-12-08 2015-09-01 Biohelix Corporation Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
WO2012051199A2 (en) 2010-10-12 2012-04-19 Monsanto Technology Llc Soybean plant and seed corresponding to transgenic event mon87712 and methods for detection thereof
WO2013003558A1 (en) 2011-06-30 2013-01-03 Monsanto Technology Llc Alfalfa plant and seed corresponding to transgenic event kk 179-2 and methods for detection thereof
US9352312B2 (en) 2011-09-23 2016-05-31 Alere Switzerland Gmbh System and apparatus for reactions
AU2013308647B2 (en) 2012-08-30 2018-10-18 Gen-Probe Incorporated Multiphase nucleic acid amplification
WO2014164479A1 (en) 2013-03-11 2014-10-09 Elitech Holding B.V. Methods for true isothermal strand displacement amplification
AU2014248813B2 (en) 2013-03-11 2016-05-19 Cue Health Inc. Systems and methods for detection and quantification of analytes
US10975423B2 (en) 2013-03-11 2021-04-13 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
US10590474B2 (en) 2013-03-11 2020-03-17 Elitechgroup B.V. Methods for true isothermal strand displacement amplification
US9623409B2 (en) 2013-03-11 2017-04-18 Cue Inc. Cartridges, kits, and methods for enhanced mixing for detection and quantification of analytes
US10545161B2 (en) 2013-03-11 2020-01-28 Cue Health Inc. Systems and methods for detection and quantification of analytes
CA2908028C (en) 2013-04-01 2022-10-18 Becton, Dickinson And Company Methods and kits for the diagnosis of influenza
CN105611827A (zh) 2013-10-09 2016-05-25 孟山都技术公司 转基因玉米事件mon87403和其检测方法
US10260111B1 (en) 2014-01-20 2019-04-16 Brett Eric Etchebarne Method of detecting sepsis-related microorganisms and detecting antibiotic-resistant sepsis-related microorganisms in a fluid sample
USD745423S1 (en) 2014-05-12 2015-12-15 Cue Inc. Automated analyzer test cartridge and sample collection device for analyte detection
WO2016149837A1 (en) 2015-03-25 2016-09-29 Axela Inc. Solid phase nucleic acid target capture and replication using strand displacing polymerases
AU2016252998B2 (en) 2015-04-24 2021-11-04 Atila Biosystems Incorporated Amplification with primers of limited nucleotide composition
US11015217B2 (en) 2015-05-29 2021-05-25 Roche Molecular Systems, Inc. Method of inactivating microbes by citraconic anhydride
EP3325153B1 (en) 2015-07-17 2020-03-18 Cue Health Inc. Sample analysis cartridge
WO2018140540A1 (en) 2017-01-25 2018-08-02 Cue Health Inc. Systems and methods for enhanced detection and quantification of analytes
US11066691B1 (en) 2018-09-14 2021-07-20 National Technology & Engineering Solutions Of Sandia, Llc Therapeutic phages and methods thereof
WO2021080629A1 (en) 2019-10-23 2021-04-29 Elitechgroup, Inc. Methods for true isothermal strand displacement amplification
CN112553350A (zh) * 2020-11-13 2021-03-26 吴涛 一种能快速检测多种分枝杆菌的方法
CN116837119A (zh) * 2022-03-25 2023-10-03 湖州申科生物技术股份有限公司 检测分枝杆菌dna的引物及检测方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
CA1339731C (en) * 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5213961A (en) * 1989-08-31 1993-05-25 Brigham And Women's Hospital Accurate quantitation of RNA and DNA by competetitive polymerase chain reaction
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
US5595891A (en) * 1990-07-19 1997-01-21 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
JPH05507206A (ja) * 1990-12-20 1993-10-21 エフ．ホフマン　―　ラ　ロシュ　アーゲー レジオネラ種の検出用ｐｃｒプライマーおよびハイブリダイゼーションアッセイにおける光学的強度の調整方法
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU652214B2 (en) * 1991-01-31 1994-08-18 Becton Dickinson & Company Strand displacement amplification "Sealing Clerk - sealing is to be refunded"
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
EP0525882B1 (en) * 1991-08-02 2004-01-14 bioMerieux B.V. Quantification of nucleic acid
ZA927323B (en) * 1991-09-24 1993-08-30 Keygene Nv Selective restriction fragment amplification: A general method for DNA fingerprinting
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5457027A (en) 1993-05-05 1995-10-10 Becton, Dickinson And Company Internal controls for isothermal nucleic acid amplification reactions
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets

Also Published As

Publication number Publication date
DE69426956T2 (de) 2001-08-16
JPH07163396A (ja) 1995-06-27
EP0640691A3 (en) 1995-07-12
AU686169B2 (en) 1998-02-05
US5736365A (en) 1998-04-07
EP0640691A2 (en) 1995-03-01
US5470723A (en) 1995-11-28
DE69426956D1 (de) 2001-05-03
SG44897A1 (en) 1997-12-19
AU6888894A (en) 1995-03-09
BR9403324A (pt) 1995-04-11
JP2814422B2 (ja) 1998-10-22
CA2129690A1 (en) 1995-02-25
EP0640691B1 (en) 2001-03-28
US5561044A (en) 1996-10-01

Similar Documents

Publication Publication Date Title
ES2157230T3 (es) Deteccion de mycobacteria por amplificacion multiple de acidos nucleicos.
ATE236269T1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
DE69430909T2 (de) Dns-sequenzbestimmung durch massenspektrometrie auf dem weg des abbaus mit exonuklease
ATE497018T1 (de) Materialien und methoden zum nachweis von nukleinsäuren
ATE346954T1 (de) Nachweis von mycobacterium tuberculosis durch vervielfältigung von nukleinsäuresequenzen
ES2194957T3 (es) Metodo para el analisis en serie de expresion genica.
DK0713921T3 (da) Fremgangsmåde til dæmpning af fluorescensen i opløsning af fluorescensmærkede oligonukleotidprober
BR9507343A (pt) Testes de hibridização - ligação para a detecção de sequências de ácido nucléico específicas
BR9908560A (pt) Detecção de microorganismos relacionados à fermentação
ES2006377A6 (es) Un metodo de analisis de acidos nucleicos por hibridacion.
ATE534749T1 (de) Sonde und primer zum nachweis von tuberkelbazillen sowie verfahren zum nachweis von tuberkelbazillen beim menschen damit
FI923660A (fi) Mycobacterium-primers och sonder
AR001619A1 (es) Método para amplificacion de secuencias especificas de acido nucleico
DK1071797T3 (da) Fremgangsmåde til isolering af et polynukleotid af interesse ud fra genomet af en mycobakterie under anvendelse af et BAC-baseret DNA-bibliotek. Anvendelse til påvisning af mycobakterier
ATE222606T1 (de) Multiplex-pcr zum nachweis von infektionen verursacht durch mycobacterium avium komplex
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
ES2080807T3 (es) Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos.
BR9606602A (pt) Primer de amplificação oligonucleotídeo e método para ampliação específica comlexa de um ácido
DE69834506D1 (de) Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung
EP0398677A3 (en) Dna hybridization probes for identification of mycobacteria
DK1338657T3 (da) Specifikt fra genomet af Mycobacterium tuberculosis-deleterede sekvenser og anvendelse heraf i diagnostik og som vacciner
WO1991014002A3 (en) Method for diagnosis of lyme disease
ATE431432T1 (de) Verfahren zum nachweis von mycobacterium tuberculosis
EP2480685B1 (en) Method for monitoring the bisulfite-mediated conversion of dna
ATE420957T1 (de) Verfahren zum nachweis des gens einer beta-3- adrenalinrezeptor-mutante sowie nukleinsäuresonde und kit dafür

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 640691

Country of ref document: ES