ES2140870T3 - Deteccion de potenciales transmembrana por metodos opticos. - Google Patents

Deteccion de potenciales transmembrana por metodos opticos.

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Publication number
ES2140870T3
ES2140870T3 ES96921410T ES96921410T ES2140870T3 ES 2140870 T3 ES2140870 T3 ES 2140870T3 ES 96921410 T ES96921410 T ES 96921410T ES 96921410 T ES96921410 T ES 96921410T ES 2140870 T3 ES2140870 T3 ES 2140870T3
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Spain
Prior art keywords
membrane
energy
transfer
reagent
potential
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Expired - Lifetime
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ES96921410T
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English (en)
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ES2140870T5 (es
Inventor
Roger Y Tsien
Jesus E Gonzalez Iii
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University of California
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University of California
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Application filed by University of California filed Critical University of California
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    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
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  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)

Abstract

SE PRESENTAN LOS METODOS Y LAS COMPOSICIONES PARA DETERMINAR EL POTENCIAL DE UNA MEMBRANA. EN UN ASPECTO, EL METODO CONSISTE EN: A) LA INTRODUCCION DE UN PRIMER REACTIVO FORMADO POR UN ION HIDROFOBO FLUORESCENTE CAPAZ DE REDISTRIBUIRSE DESDE UN PRIMER LADO DE LA MEMBRANA A UN SEGUNDO LADO DE LA MEMBRANA COMO RESPUESTA A CAMBIOS EN EL POTENCIAL DE LA MEMBRANA, SEGUN SE DESCRIBE CON LA ECUACION DE NERST; B) LA INTRODUCCION DE UN SEGUNDO REACTIVO QUE MARCA EL PRIMER O EL SEGUNDO LADO DE LA MEMBRANA, ESTANDO FORMADO ESTE SEGUNDO REACTIVO POR UN CROMOFORO CAPAZ DE TRANSFERIR ENERGIA MEDIANTE I) LA DONACION DE LA ENERGIA EN ESTADO EXCITADO AL ION FLUORESCENTE, O II) LA ACEPTACION DE LA ENERGIA DEL ESTADO EXCITADO PROCEDENTE DEL ION FLUORESCENTE; C) LA EXPOSICION DE LA MEMBRANA A LA RADIACION; D) LA DETERMINACION DE LA TRANSFERENCIA DE ENERGIA ENTRE EL ION FLUORESCENTE Y EL SEGUNDO REACTIVO; Y E) RELACIONAR LA TRANSFERENCIA DE ENERGIA CON EL POTENCIAL DE MEMBRANA. LA TRANSFERENCIA DE ENERGIA SE MIDE TIPICAMENTE MEDIANTE LA TRANSFERENCIA DE LA ENERGIA DE RESONANCIA DE FLUORESCENCIA. EN ALGUNAS REALIZACIONES EL PRIMER Y SEGUNDO REACTIVOS ESTAN UNIDOS MEDIANTE UN VINCULO ADECUADO. EN UNA REALIZACION, EL METODO SE UTILIZA PARA IDENTIFICAR COMPUESTOS QUE MODULEN LOS POTENCIALES DE MEMBRANA EN MEMBRANAS BIOLOGICAS.
ES96921410T 1995-06-07 1996-06-06 Deteccion de potenciales transmembrana por metodos opticos. Expired - Lifetime ES2140870T5 (es)

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US08/481,977 US5661035A (en) 1995-06-07 1995-06-07 Voltage sensing by fluorescence resonance energy transfer

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AU716130B2 (en) 2000-02-17
DE69605041T3 (de) 2006-09-21
DE69605041D1 (de) 1999-12-09
JPH11508355A (ja) 1999-07-21
HK1090422A1 (en) 2006-12-22
AU6264396A (en) 1996-12-30
US5661035A (en) 1997-08-26
EP1610126B1 (en) 2014-04-30
EP0834074A1 (en) 1998-04-08
EP0977035B2 (en) 2010-07-21
CA2223927A1 (en) 1996-12-19
EP0977035A2 (en) 2000-02-02
EP0834074B1 (en) 1999-11-03
EP1610126A1 (en) 2005-12-28
DE69635193D1 (de) 2005-10-20
DE69605041T2 (de) 2000-03-16
EP0977035B1 (en) 2005-09-14
WO1996041166A2 (en) 1996-12-19
DE69635193T3 (de) 2011-03-10
DK0834074T4 (da) 2005-12-12
DK0834074T3 (da) 2000-04-03
WO1996041166A3 (en) 1997-05-15
DE69635193T2 (de) 2006-01-19
EP0834074B2 (en) 2005-11-16
ES2140870T5 (es) 2006-04-16
CA2223927C (en) 2004-04-06
JP4033899B2 (ja) 2008-01-16
US6107066A (en) 2000-08-22
JP2004231666A (ja) 2004-08-19
JP4064945B2 (ja) 2008-03-19
ATE186400T1 (de) 1999-11-15
EP0977035A3 (en) 2000-03-01
ATE304706T1 (de) 2005-09-15

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