DE69605041D1 - Erkennung von transmembranpotentialen durch optische verfahren - Google Patents

Erkennung von transmembranpotentialen durch optische verfahren

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Publication number
DE69605041D1
DE69605041D1 DE69605041T DE69605041T DE69605041D1 DE 69605041 D1 DE69605041 D1 DE 69605041D1 DE 69605041 T DE69605041 T DE 69605041T DE 69605041 T DE69605041 T DE 69605041T DE 69605041 D1 DE69605041 D1 DE 69605041D1
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Germany
Prior art keywords
membrane
energy transfer
face
reagent
potential
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Expired - Lifetime
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DE69605041T
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English (en)
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DE69605041T2 (de
DE69605041T3 (de
Inventor
Roger Tsien
Jesus Gonzalez
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University of California
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University of California
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23914150&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69605041(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of California filed Critical University of California
Publication of DE69605041D1 publication Critical patent/DE69605041D1/de
Publication of DE69605041T2 publication Critical patent/DE69605041T2/de
Application granted granted Critical
Publication of DE69605041T3 publication Critical patent/DE69605041T3/de
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DE69605041T 1995-06-07 1996-06-06 Erkennung von transmembranpotentialen durch optische verfahren Expired - Lifetime DE69605041T3 (de)

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US08/481,977 US5661035A (en) 1995-06-07 1995-06-07 Voltage sensing by fluorescence resonance energy transfer
PCT/US1996/009652 WO1996041166A2 (en) 1995-06-07 1996-06-06 Detection of transmembrane potentials by optical methods

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Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US5661035A (en) * 1995-06-07 1997-08-26 The Regents Of The University Of California Voltage sensing by fluorescence resonance energy transfer
US6596522B2 (en) * 1997-05-08 2003-07-22 The Regents Of The University Of California Detection of transmembrane potentials by optical methods
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US6342379B1 (en) * 1995-06-07 2002-01-29 The Regents Of The University Of California Detection of transmembrane potentials by optical methods
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US6593135B2 (en) * 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
AT405103B (de) * 1996-10-16 1999-05-25 Avl Verbrennungskraft Messtech Sensorschicht zur quantitativen bestimmung zumindest einer chemischen komponente einer gasförmigen oder flüssigen probe
FR2756633B1 (fr) * 1996-11-29 1999-01-15 Centre Nat Rech Scient Methode de detection in vitro du potentiel transmembranaire mitochondrial
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
FR2764387B1 (fr) 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US6221612B1 (en) * 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6348322B1 (en) * 1997-10-17 2002-02-19 Duke University Method of screening for specific binding interactions
US7244349B2 (en) * 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
EP1053253A4 (de) * 1998-02-05 2004-09-29 Merck & Co Inc Neue dns-sequenzen, die für einen gaba b-rezeptor kodieren.
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
US6388788B1 (en) 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
US6083486A (en) * 1998-05-14 2000-07-04 The General Hospital Corporation Intramolecularly-quenched near infrared fluorescent probes
US6592847B1 (en) * 1998-05-14 2003-07-15 The General Hospital Corporation Intramolecularly-quenched near infrared flourescent probes
AU4188599A (en) * 1998-05-15 1999-12-06 Geron Corporation Method and apparatus for detecting hazardous agents
US5998146A (en) * 1998-07-17 1999-12-07 Wallac Oy Homogeneous luminescence assay method based on energy transfer
ATE240310T1 (de) * 1998-12-14 2003-05-15 Vertex Pharma San Diego Llc Optische molekularsensoren für cytochrome p-450 aktivität
US6420130B1 (en) * 1998-12-14 2002-07-16 Aurora Biosciences Corporation Optical molecular sensors for cytochrome P450 activity
US8148110B2 (en) 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
US6285909B1 (en) 1999-05-27 2001-09-04 Cardiac Pacemakers, Inc. Preserving patient specific data in implantable pulse generator systems
US6662039B2 (en) * 1999-06-18 2003-12-09 The Trustees Of Columbia University In The City Of New York Optical probing of neuronal connections with fluorescent indicators
JP2003506014A (ja) * 1999-06-22 2003-02-18 マイトコー エネルギー移動を使用する亜細胞条件およびプロセスをアッセイするための組成物および方法
US6323039B1 (en) 1999-06-22 2001-11-27 Mitokor Compositions and methods for assaying subcellular conditions and processes using energy transfer
CA2379580A1 (en) * 1999-07-20 2001-01-25 Merck & Co., Inc. Novel human calcium sensitive potassium channel subunits
US6358684B1 (en) 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
ES2214781T3 (es) * 1999-09-01 2004-09-16 Invitrogen Corporation Agentes fotorreductores para ensayos de fluorescencia.
US6468763B1 (en) 1999-09-29 2002-10-22 Caliper Technologies Corp. Optical detection of transmembrane potential changes
WO2001027253A1 (en) * 1999-10-08 2001-04-19 Caliper Technologies Corp. Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
US6740466B1 (en) * 1999-11-15 2004-05-25 Fuji Photo Film Co., Ltd. Photopolymerizable composition and recording material using the same
GB0003069D0 (en) * 2000-02-11 2000-03-29 Cambridge Drug Discovery Ltd Improved assay
US6287758B1 (en) * 2000-03-23 2001-09-11 Axiom Biotechnologies, Inc. Methods of registering trans-membrane electric potentials
EP1294751B1 (de) 2000-03-31 2014-03-05 Merial Limited Gaba-abhängige chloridkanäle aus dermacentor variabilis
US7399599B2 (en) 2000-07-10 2008-07-15 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
JP5603850B2 (ja) * 2000-07-10 2014-10-08 ヴァーテックス ファーマシューティカルズ （サンディエゴ）エルエルシー イオンチャネルアッセイ法
WO2002008748A2 (en) * 2000-07-10 2002-01-31 Aurora Biosciences Corporation Ion channel assay methods
US7615356B2 (en) 2000-07-10 2009-11-10 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
AU3932902A (en) * 2000-11-22 2002-06-03 Allergan Sales Inc A high-throughput screen for identifying channel blockers that selectively distinguish transient from persistent sodium channels
US7383076B2 (en) * 2000-11-27 2008-06-03 The General Hospital Corporation Fluorescence-mediated molecular tomography
US20030044353A1 (en) * 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
US20030211575A1 (en) * 2001-02-07 2003-11-13 Swartz Kenton J. Constituitively open voltage-gated kand methods for discovering modulators thereof
US7005511B2 (en) 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
US20060029955A1 (en) * 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
US20050058990A1 (en) * 2001-03-24 2005-03-17 Antonio Guia Biochip devices for ion transport measurement, methods of manufacture, and methods of use
WO2002077259A2 (en) * 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20050196746A1 (en) * 2001-03-24 2005-09-08 Jia Xu High-density ion transport measurement biochip devices and methods
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
CA2448719A1 (en) * 2001-06-09 2002-12-19 Glsynthesis Inc. Lipid structures and uses thereof
DE10130172A1 (de) * 2001-06-22 2003-01-09 Evotec Ag Fluoreszenz-Farbstoffderivate
FR2826666B1 (fr) * 2001-07-02 2004-07-02 Novaleads Procede de selection d'agents bio-actifs par couplage de luminescence et systeme cellulaire vivant pour la mise en oeuvre de ce procede
WO2003006103A2 (en) * 2001-07-12 2003-01-23 Merck & Co., Inc. Electrical field stimulation of eukaryotic cells
US7361478B2 (en) 2001-11-20 2008-04-22 Allergan, Inc. High-throughput screen for identifying selective persistent sodium channels channel blockers
US20070197583A1 (en) * 2002-03-29 2007-08-23 Welsh Michael J Novel compositions and methods for modulation of the acid-sensing ion channel (ASIC) for the treatment of anxiety and drug addiction
US20030186860A1 (en) * 2002-03-29 2003-10-02 Welsh Michael J. Novel compositions and methods for modulating the acid-sensing ion channel (ASIC)
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
EP1501924A4 (de) * 2002-05-04 2006-05-24 Aviva Biosciences Corp Vorrichtung mit strukturen nachweisendem ionentransport und verwendungsverfahren
US20030228566A1 (en) * 2002-06-11 2003-12-11 Biotechplex Corporation Method of and apparatus for screening for drug candidates
US7427489B1 (en) * 2002-06-17 2008-09-23 The Scripps Research Institute Screening assay to identify modulators of the sleep/wake cycle
CA2501233A1 (en) 2002-10-10 2004-04-22 Merck & Co., Inc. Assay methods for state-dependent calcium channel agonists/antagonists
US20050037364A1 (en) * 2003-08-13 2005-02-17 International Business Machines Corporation Techniques for recording signals
US7125908B2 (en) * 2003-08-29 2006-10-24 Allergan, Inc. Treating pain using selective antagonists of persistent sodium current
US7060723B2 (en) * 2003-08-29 2006-06-13 Allergan, Inc. Treating neurological disorders using selective antagonists of persistent sodium current
US8329390B2 (en) * 2004-10-21 2012-12-11 Anaspec Incorporated Detection of transmembrane potentials using N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonols
EP1828830A1 (de) 2004-12-21 2007-09-05 Cedars-Sinai Medical Center Verfahren zum screening und zur charakterisierung von arzneimitteln durch ionenstromanalyse
FR2890174B1 (fr) * 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
EP1934610B1 (de) * 2005-10-12 2010-01-27 Allergan, Inc. Tests der molekularen oder subzellulären interaktivität unter verwendung von depolarisierung nach resonanzenergietransfer (daret)
WO2007122602A1 (en) * 2006-04-26 2007-11-01 Seng Enterprises Ltd. A method and system for measuring membrane potential based on fluorescence polarization
US20110098309A1 (en) * 2007-07-12 2011-04-28 Acumen Pharmaceuticals, Inc. Methods of inhibiting the formation of amyloid-beta diffusable ligands using acylhydrazide compounds
US9006283B2 (en) * 2007-07-12 2015-04-14 Acumen Pharmaceuticals, Inc. Methods of modifying amyloid β oligomers using non-peptidic compounds
US8962677B2 (en) 2007-07-12 2015-02-24 Acumen Pharmaceuticals, Inc. Methods of restoring cognitive ability using non-peptidic compounds
DK2244741T3 (en) 2008-01-18 2015-05-26 Visen Medical Inc Fluorescent imaging agents
US20090291150A1 (en) * 2008-05-21 2009-11-26 University Of Iowa Research Foundation Method and compositions for treating and preventing seizures by modulating acid-sensing ion channel activity
US8962341B2 (en) 2008-12-12 2015-02-24 The Commonwealth Medical College Cell-based detection of APF through its interaction with CKAP4 for diagnosis of interstitial cystitis
WO2010117420A2 (en) * 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US9222043B2 (en) * 2009-09-22 2015-12-29 Authentix, Inc. Dipyrromethenes and azadipyrromethenes as markers for petroleum products
CN103168236B (zh) 2010-08-23 2016-01-20 哈佛大学管理委员会 用于膜电位测定的光遗传学探针
US9207237B2 (en) 2010-08-23 2015-12-08 President And Fellows Of Harvard College Systems, methods, and workflows for optogenetics analysis
WO2013130808A1 (en) 2012-02-29 2013-09-06 D.E. Shaw Research, Llc Methods for screening voltage gated proteins
US9682970B2 (en) 2012-06-29 2017-06-20 Biotium, Inc. Fluorescent compounds and uses thereof
KR101933620B1 (ko) * 2012-09-18 2018-12-28 삼성전자주식회사 소포를 검출하기 위한 조성물, 키트 및 이를 이용하여 소포를 분석하는 방법
US10175174B2 (en) 2012-12-11 2019-01-08 Ge Healthcare Bio-Sciences Corp. Photoactivated chemical bleaching of dyes
JP6321634B2 (ja) 2013-05-23 2018-05-09 学校法人慶應義塾 光第二高調波発生化合物、光第二高調波発生色素組成物及び細胞検査方法
US9063420B2 (en) 2013-07-16 2015-06-23 Rohm And Haas Electronic Materials Llc Photoresist composition, coated substrate, and method of forming electronic device
US8962779B2 (en) 2013-07-16 2015-02-24 Dow Global Technologies Llc Method of forming polyaryl polymers
US9410016B2 (en) 2013-07-16 2016-08-09 Dow Global Technologies Llc Aromatic polyacetals and articles comprising them
US8933239B1 (en) 2013-07-16 2015-01-13 Dow Global Technologies Llc Bis(aryl)acetal compounds
WO2015195769A2 (en) 2014-06-18 2015-12-23 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
CN104357045B (zh) * 2014-11-05 2016-06-01 北京化工大学 具有极酸/极碱开关响应的螺吡喃小分子荧光探针的合成方法及其应用
US10077463B2 (en) 2015-01-15 2018-09-18 President And Fellows Of Harvard College Optical selection of cells
US11536716B2 (en) * 2015-06-25 2022-12-27 Hoffmann-La Roche Inc. Cell based assay for determining antibody or ligand binding and function
WO2018217266A1 (en) 2017-03-02 2018-11-29 Akita Innovations Llc Voltage sensitive dyes
US11293927B2 (en) 2018-04-30 2022-04-05 Ribon Therapeutics, Inc. Screening methods for PARP modulators
WO2020018700A1 (en) 2018-07-18 2020-01-23 Manzanita Pharmaceuticals, Inc. Conjugates for delivering an anti-cancer agent to nerve cells, methods of use and methods of making thereof
RU197959U1 (ru) * 2020-01-28 2020-06-09 Федеральное государственное бюджетное образовательное учреждение высшего образования "МИРЭА - Российский технологический университет" Устройство для исследования компонент вещества с помощью микроволновой плазмы методами оптической и ямр спектроскопии
WO2022008720A1 (en) * 2020-07-10 2022-01-13 Life & Brain Gmbh Novel hybrid optical voltage sensors

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1597482B2 (de) * 1967-09-22 1977-03-24 Agfa-Gevaert Ag, 5090 Leverkusen Lichtempfindliches photographisches aufzeichnungsmaterial mit lichthofschutz- oder filterfarbstoffen
JPS59218956A (ja) * 1983-05-27 1984-12-10 Fujirebio Inc 膜融合測定方法
ES8607563A1 (es) * 1983-10-13 1986-06-01 Univ Georgia Res Found Un metodo para detectar una interaccion ligante
US4647531A (en) * 1984-02-06 1987-03-03 Ortho Diagnostic Systems, Inc. Generalized cytometry instrument and methods of use
US4861727A (en) * 1986-09-08 1989-08-29 C. R. Bard, Inc. Luminescent oxygen sensor based on a lanthanide complex
US4900934A (en) * 1987-07-15 1990-02-13 University Of Utah Apparatus for simultaneous visualization and measurement of fluorescence from fluorescent dye-treated cell preparations and solutions
AT393035B (de) * 1989-04-25 1991-07-25 Avl Verbrennungskraft Messtech Verfahren zur quantitativen bestimmung zumindest eines chemischen parameters eines probenmediums
US5169788A (en) * 1989-06-09 1992-12-08 New England Deaconess Hospital Corporation Methods of measuring membrane potential using j-aggregate forming dyes
DK0429907T3 (da) * 1989-11-21 1994-10-03 Bayer Ag Optisk biosensor
US5244813A (en) * 1991-01-25 1993-09-14 Trustees Of Tufts College Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample
DE4121426A1 (de) * 1991-06-28 1993-01-14 Basf Ag Chemischer sensor
CA2087412A1 (en) * 1992-01-17 1993-07-18 Joseph R. Lakowicz Ph and pco2 sensing by luminescent lifetimes and energy transfer
WO1995008637A1 (en) * 1993-09-21 1995-03-30 Washington State University Research Foundation Immunoassay comprising ligand-conjugated, ion channel receptor immobilized in lipid film
GB9406464D0 (en) * 1994-03-31 1994-05-25 British Tech Group Assay for determining biochemical changes at phospholipid bilayer membrane surfaces
US5661035A (en) * 1995-06-07 1997-08-26 The Regents Of The University Of California Voltage sensing by fluorescence resonance energy transfer

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ATE304706T1 (de) 2005-09-15
AU6264396A (en) 1996-12-30
JP4064945B2 (ja) 2008-03-19
EP0834074B2 (de) 2005-11-16
DE69635193T3 (de) 2011-03-10
EP0977035B1 (de) 2005-09-14
CA2223927C (en) 2004-04-06
DK0834074T4 (da) 2005-12-12
EP0834074B1 (de) 1999-11-03
DK0834074T3 (da) 2000-04-03
EP1610126B1 (de) 2014-04-30
EP0977035A2 (de) 2000-02-02
ES2140870T3 (es) 2000-03-01
AU716130B2 (en) 2000-02-17
DE69605041T2 (de) 2000-03-16
EP1610126A1 (de) 2005-12-28
DE69635193T2 (de) 2006-01-19
US5661035A (en) 1997-08-26
EP0977035B2 (de) 2010-07-21
ES2140870T5 (es) 2006-04-16
WO1996041166A3 (en) 1997-05-15
HK1090422A1 (en) 2006-12-22
DE69605041T3 (de) 2006-09-21
JPH11508355A (ja) 1999-07-21
WO1996041166A2 (en) 1996-12-19
DE69635193D1 (de) 2005-10-20
JP2004231666A (ja) 2004-08-19
EP0834074A1 (de) 1998-04-08
EP0977035A3 (de) 2000-03-01
CA2223927A1 (en) 1996-12-19
JP4033899B2 (ja) 2008-01-16
US6107066A (en) 2000-08-22
ATE186400T1 (de) 1999-11-15

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