DE69635193D1 - System zur Messung des elektrischen Potentials durch eine Membrane - Google Patents

System zur Messung des elektrischen Potentials durch eine Membrane

Info

Publication number
DE69635193D1
DE69635193D1 DE69635193T DE69635193T DE69635193D1 DE 69635193 D1 DE69635193 D1 DE 69635193D1 DE 69635193 T DE69635193 T DE 69635193T DE 69635193 T DE69635193 T DE 69635193T DE 69635193 D1 DE69635193 D1 DE 69635193D1
Authority
DE
Germany
Prior art keywords
membrane
energy transfer
face
reagent
potential
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69635193T
Other languages
English (en)
Other versions
DE69635193T2 (de
DE69635193T3 (de
Inventor
Roger Y Tsien
Jesus E Gonzalez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California
Original Assignee
University of California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23914150&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69635193(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by University of California filed Critical University of California
Application granted granted Critical
Publication of DE69635193D1 publication Critical patent/DE69635193D1/de
Publication of DE69635193T2 publication Critical patent/DE69635193T2/de
Publication of DE69635193T3 publication Critical patent/DE69635193T3/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/08—Indoles; Hydrogenated indoles with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, directly attached to carbon atoms of the hetero ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
    • C07D239/60—Three or more oxygen or sulfur atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
    • C07D239/60—Three or more oxygen or sulfur atoms
    • C07D239/62—Barbituric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
    • C07D239/60—Three or more oxygen or sulfur atoms
    • C07D239/66—Thiobarbituric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D311/00—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings
    • C07D311/02—Heterocyclic compounds containing six-membered rings having one oxygen atom as the only hetero atom, condensed with other rings ortho- or peri-condensed with carbocyclic rings or ring systems
    • C07D311/04—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring
    • C07D311/06—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2
    • C07D311/08—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring
    • C07D311/16—Benzo[b]pyrans, not hydrogenated in the carbocyclic ring with oxygen or sulfur atoms directly attached in position 2 not hydrogenated in the hetero ring substituted in position 7
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F5/00—Compounds containing elements of Groups 3 or 13 of the Periodic Table
    • C07F5/003—Compounds containing elements of Groups 3 or 13 of the Periodic Table without C-Metal linkages
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F5/00—Compounds containing elements of Groups 3 or 13 of the Periodic Table
    • C07F5/02—Boron compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F5/00—Compounds containing elements of Groups 3 or 13 of the Periodic Table
    • C07F5/02—Boron compounds
    • C07F5/027—Organoboranes and organoborohydrides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/10—Phosphatides, e.g. lecithin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
    • C07F9/553—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom having one nitrogen atom as the only ring hetero atom
    • C07F9/572—Five-membered rings
    • C07F9/5728—Five-membered rings condensed with carbocyclic rings or carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058—Neurological cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5061—Muscle cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6872—Intracellular protein regulatory factors and their receptors, e.g. including ion channels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Instrument Details And Bridges, And Automatic Balancing Devices (AREA)
  • Traffic Control Systems (AREA)
  • Image Input (AREA)
  • Measurement Of Current Or Voltage (AREA)
  • Photometry And Measurement Of Optical Pulse Characteristics (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Elimination Of Static Electricity (AREA)
  • Electrostatic Charge, Transfer And Separation In Electrography (AREA)
DE69635193T 1995-06-07 1996-06-06 System zur Messung des elektrischen Potentials durch eine Membrane Expired - Lifetime DE69635193T3 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US481977 1995-06-07
US08/481,977 US5661035A (en) 1995-06-07 1995-06-07 Voltage sensing by fluorescence resonance energy transfer

Publications (3)

Publication Number Publication Date
DE69635193D1 true DE69635193D1 (de) 2005-10-20
DE69635193T2 DE69635193T2 (de) 2006-01-19
DE69635193T3 DE69635193T3 (de) 2011-03-10

Family

ID=23914150

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69635193T Expired - Lifetime DE69635193T3 (de) 1995-06-07 1996-06-06 System zur Messung des elektrischen Potentials durch eine Membrane
DE69605041T Expired - Lifetime DE69605041T3 (de) 1995-06-07 1996-06-06 Erkennung von transmembranpotentialen durch optische verfahren

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69605041T Expired - Lifetime DE69605041T3 (de) 1995-06-07 1996-06-06 Erkennung von transmembranpotentialen durch optische verfahren

Country Status (11)

Country Link
US (2) US5661035A (de)
EP (3) EP1610126B1 (de)
JP (2) JP4033899B2 (de)
AT (2) ATE304706T1 (de)
AU (1) AU716130B2 (de)
CA (1) CA2223927C (de)
DE (2) DE69635193T3 (de)
DK (1) DK0834074T4 (de)
ES (1) ES2140870T5 (de)
HK (1) HK1090422A1 (de)
WO (1) WO1996041166A2 (de)

Families Citing this family (103)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028190A (en) 1994-02-01 2000-02-22 The Regents Of The University Of California Probes labeled with energy transfer coupled dyes
US6596522B2 (en) * 1997-05-08 2003-07-22 The Regents Of The University Of California Detection of transmembrane potentials by optical methods
US5661035A (en) * 1995-06-07 1997-08-26 The Regents Of The University Of California Voltage sensing by fluorescence resonance energy transfer
US6342379B1 (en) * 1995-06-07 2002-01-29 The Regents Of The University Of California Detection of transmembrane potentials by optical methods
US6008373A (en) 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5945526A (en) 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US7388092B2 (en) 1996-05-03 2008-06-17 Applera Corporation Oligonucleotides and analogs labeled with energy transfer dyes
US6593135B2 (en) * 1996-08-16 2003-07-15 The State Of Oregon, Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Long wavelength engineered fluorescent proteins
AT405103B (de) * 1996-10-16 1999-05-25 Avl Verbrennungskraft Messtech Sensorschicht zur quantitativen bestimmung zumindest einer chemischen komponente einer gasförmigen oder flüssigen probe
FR2756633B1 (fr) * 1996-11-29 1999-01-15 Centre Nat Rech Scient Methode de detection in vitro du potentiel transmembranaire mitochondrial
US6342345B1 (en) 1997-04-02 2002-01-29 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by reporter subunit complementation
FR2764387B1 (fr) * 1997-06-05 1999-07-23 Centre Nat Rech Scient Utilisation d'une proteine fluorescente pour la detection d'interactions entre une proteine cible et son ligand
US6221612B1 (en) * 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6348322B1 (en) * 1997-10-17 2002-02-19 Duke University Method of screening for specific binding interactions
US7244349B2 (en) * 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
WO1999040114A1 (en) * 1998-02-05 1999-08-12 Merck & Co., Inc. Novel gabab receptor dna sequences
US6388788B1 (en) 1998-03-16 2002-05-14 Praelux, Inc. Method and apparatus for screening chemical compounds
US20030036855A1 (en) * 1998-03-16 2003-02-20 Praelux Incorporated, A Corporation Of New Jersey Method and apparatus for screening chemical compounds
US6083486A (en) * 1998-05-14 2000-07-04 The General Hospital Corporation Intramolecularly-quenched near infrared fluorescent probes
US6592847B1 (en) * 1998-05-14 2003-07-15 The General Hospital Corporation Intramolecularly-quenched near infrared flourescent probes
WO1999060371A2 (en) * 1998-05-15 1999-11-25 Geron Corporation Method and apparatus for detecting hazardous agents
US5998146A (en) * 1998-07-17 1999-12-07 Wallac Oy Homogeneous luminescence assay method based on energy transfer
US6420130B1 (en) * 1998-12-14 2002-07-16 Aurora Biosciences Corporation Optical molecular sensors for cytochrome P450 activity
PT1140888E (pt) * 1998-12-14 2003-09-30 Vertex Pharma San Diego Llc Sensores opticos moleculares para a actividade do citocromo p450
US8148110B2 (en) 1999-03-15 2012-04-03 The Board Of Trustees Of The Leland Stanford Junior University Detection of molecular interactions by β-lactamase reporter fragment complementation
US6285909B1 (en) 1999-05-27 2001-09-04 Cardiac Pacemakers, Inc. Preserving patient specific data in implantable pulse generator systems
US6662039B2 (en) * 1999-06-18 2003-12-09 The Trustees Of Columbia University In The City Of New York Optical probing of neuronal connections with fluorescent indicators
US6323039B1 (en) 1999-06-22 2001-11-27 Mitokor Compositions and methods for assaying subcellular conditions and processes using energy transfer
AU5763600A (en) * 1999-06-22 2001-01-09 Mitokor Compositions and methods for assaying subcellular conditions and processes usingenergy transfer
CA2379580A1 (en) * 1999-07-20 2001-01-25 Merck & Co., Inc. Novel human calcium sensitive potassium channel subunits
US6358684B1 (en) 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
PT1081495E (pt) * 1999-09-01 2004-06-30 Invitrogen Corp Agentes de reducao de fotoes para ensaios de fluorescencia
US6468763B1 (en) 1999-09-29 2002-10-22 Caliper Technologies Corp. Optical detection of transmembrane potential changes
AU783191B2 (en) * 1999-10-08 2005-10-06 Caliper Life Sciences, Inc. Use of nernstein voltage sensitive dyes in measuring transmembrane voltage
US6740466B1 (en) * 1999-11-15 2004-05-25 Fuji Photo Film Co., Ltd. Photopolymerizable composition and recording material using the same
GB0003069D0 (en) * 2000-02-11 2000-03-29 Cambridge Drug Discovery Ltd Improved assay
US6287758B1 (en) * 2000-03-23 2001-09-11 Axiom Biotechnologies, Inc. Methods of registering trans-membrane electric potentials
US7220832B2 (en) * 2000-03-31 2007-05-22 Merck & Co., Inc Dermacentor variabilis GABA-gated chloride channels
US7615356B2 (en) 2000-07-10 2009-11-10 Vertex Pharmaceuticals (San Diego) Llc Ion channel assay methods
JP5603850B2 (ja) * 2000-07-10 2014-10-08 ヴァーテックス ファーマシューティカルズ （サンディエゴ）エルエルシー イオンチャネルアッセイ法
EP1303757B1 (de) * 2000-07-10 2006-10-11 Vertex Pharmaceuticals (San Diego) LLC Ionenkanal-testverfahren
US6969449B2 (en) 2000-07-10 2005-11-29 Vertex Pharmaceuticals (San Diego) Llc Multi-well plate and electrode assemblies for ion channel assays
JP2004531691A (ja) * 2000-11-22 2004-10-14 アラーガン、インコーポレイテッド 一過性ナトリウムチャネルと持続性ナトリウムチャネルとを選択的に識別するチャネル遮断薬を同定するためのハイスループットスクリーン
US7383076B2 (en) * 2000-11-27 2008-06-03 The General Hospital Corporation Fluorescence-mediated molecular tomography
US20030044353A1 (en) * 2001-01-05 2003-03-06 Ralph Weissleder Activatable imaging probes
US20030211575A1 (en) * 2001-02-07 2003-11-13 Swartz Kenton J. Constituitively open voltage-gated kand methods for discovering modulators thereof
US7005511B2 (en) 2001-02-26 2006-02-28 The Regents Of The University Of California Fluorescent protein variants and methods for making same
US20050196746A1 (en) * 2001-03-24 2005-09-08 Jia Xu High-density ion transport measurement biochip devices and methods
US20050058990A1 (en) * 2001-03-24 2005-03-17 Antonio Guia Biochip devices for ion transport measurement, methods of manufacture, and methods of use
US20060029955A1 (en) * 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
AU2002307218A1 (en) * 2001-03-24 2002-10-08 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20050009004A1 (en) * 2002-05-04 2005-01-13 Jia Xu Apparatus including ion transport detecting structures and methods of use
US20040146849A1 (en) * 2002-01-24 2004-07-29 Mingxian Huang Biochips including ion transport detecting structures and methods of use
WO2002101364A1 (en) * 2001-06-09 2002-12-19 Glsynthesis Inc. Lipid structures and uses thereof
DE10130172A1 (de) * 2001-06-22 2003-01-09 Evotec Ag Fluoreszenz-Farbstoffderivate
FR2826666B1 (fr) * 2001-07-02 2004-07-02 Novaleads Procede de selection d'agents bio-actifs par couplage de luminescence et systeme cellulaire vivant pour la mise en oeuvre de ce procede
AU2002354569A1 (en) * 2001-07-12 2003-01-29 Merck And Co., Inc. Electrical field stimulation of eukaryotic cells
US7361478B2 (en) 2001-11-20 2008-04-22 Allergan, Inc. High-throughput screen for identifying selective persistent sodium channels channel blockers
US20070197583A1 (en) * 2002-03-29 2007-08-23 Welsh Michael J Novel compositions and methods for modulation of the acid-sensing ion channel (ASIC) for the treatment of anxiety and drug addiction
US20030186860A1 (en) * 2002-03-29 2003-10-02 Welsh Michael J. Novel compositions and methods for modulating the acid-sensing ion channel (ASIC)
US20050181464A1 (en) * 2002-04-04 2005-08-18 Affinium Pharmaceuticals, Inc. Novel purified polypeptides from bacteria
CA2485099C (en) * 2002-05-04 2017-09-26 Aviva Biosciences Corporation Apparatus including ion transport detecting structures and methods of use
US20030228566A1 (en) * 2002-06-11 2003-12-11 Biotechplex Corporation Method of and apparatus for screening for drug candidates
US7427489B1 (en) * 2002-06-17 2008-09-23 The Scripps Research Institute Screening assay to identify modulators of the sleep/wake cycle
EP1560915B1 (de) 2002-10-10 2009-03-04 Merck & Co., Inc. Testverfahren für zustandsabhängige calciumkanal-agonisten/-antagonisten
US20050037364A1 (en) * 2003-08-13 2005-02-17 International Business Machines Corporation Techniques for recording signals
US7125908B2 (en) * 2003-08-29 2006-10-24 Allergan, Inc. Treating pain using selective antagonists of persistent sodium current
US7060723B2 (en) 2003-08-29 2006-06-13 Allergan, Inc. Treating neurological disorders using selective antagonists of persistent sodium current
US8329390B2 (en) * 2004-10-21 2012-12-11 Anaspec Incorporated Detection of transmembrane potentials using N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonols
WO2006069188A1 (en) 2004-12-21 2006-06-29 Cedars-Sinai Medical Center Method for drug screening and characterization by ion flux analysis
FR2890174B1 (fr) * 2005-08-30 2009-04-24 Cis Bio Internat Sa Procede pour la mise en evidence d'un processus biologique par mesure d'un fret
ATE555387T1 (de) * 2005-10-12 2012-05-15 Allergan Inc Tests der molekularen oder subzellulären nähe unter verwendung von depolarisierung nach resonanzenergietransfer (daret)
US20070254276A1 (en) * 2006-04-26 2007-11-01 Seng Enterprises Ltd. Method and system for measuring membrane potential based on fluorescence polarization
US20110098309A1 (en) * 2007-07-12 2011-04-28 Acumen Pharmaceuticals, Inc. Methods of inhibiting the formation of amyloid-beta diffusable ligands using acylhydrazide compounds
US8962677B2 (en) 2007-07-12 2015-02-24 Acumen Pharmaceuticals, Inc. Methods of restoring cognitive ability using non-peptidic compounds
US9006283B2 (en) * 2007-07-12 2015-04-14 Acumen Pharmaceuticals, Inc. Methods of modifying amyloid β oligomers using non-peptidic compounds
CA2749108C (en) * 2008-01-18 2017-06-27 Visen Medical, Inc. Intramolecularly-quenched fluorescent imaging agents
US20090291150A1 (en) * 2008-05-21 2009-11-26 University Of Iowa Research Foundation Method and compositions for treating and preventing seizures by modulating acid-sensing ion channel activity
CA2747244A1 (en) 2008-12-12 2010-06-17 University Of Florida Research Foundation, Inc. Cell-based detection of apf through its interaction with ckap4 for diagnosis of interstitial cystitis
WO2010117420A2 (en) * 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US9222043B2 (en) * 2009-09-22 2015-12-29 Authentix, Inc. Dipyrromethenes and azadipyrromethenes as markers for petroleum products
US9057734B2 (en) 2010-08-23 2015-06-16 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
US9207237B2 (en) 2010-08-23 2015-12-08 President And Fellows Of Harvard College Systems, methods, and workflows for optogenetics analysis
WO2013130808A1 (en) 2012-02-29 2013-09-06 D.E. Shaw Research, Llc Methods for screening voltage gated proteins
US9682970B2 (en) 2012-06-29 2017-06-20 Biotium, Inc. Fluorescent compounds and uses thereof
KR101933620B1 (ko) * 2012-09-18 2018-12-28 삼성전자주식회사 소포를 검출하기 위한 조성물, 키트 및 이를 이용하여 소포를 분석하는 방법
SG11201504129SA (en) 2012-12-11 2015-06-29 Clarient Diagnostic Services Inc Photoactivated chemical bleaching of dyes
EP3000848B1 (de) 2013-05-23 2017-10-25 Keio University Verbindung zur erzeugung einer zweiten oberschwingung von licht, färbemittelzusammensetzungen zur erzeugung einer zweiten oberschwingung von licht und zelluntersuchungsverfahren
US8933239B1 (en) 2013-07-16 2015-01-13 Dow Global Technologies Llc Bis(aryl)acetal compounds
US9063420B2 (en) 2013-07-16 2015-06-23 Rohm And Haas Electronic Materials Llc Photoresist composition, coated substrate, and method of forming electronic device
US8962779B2 (en) 2013-07-16 2015-02-24 Dow Global Technologies Llc Method of forming polyaryl polymers
US9410016B2 (en) 2013-07-16 2016-08-09 Dow Global Technologies Llc Aromatic polyacetals and articles comprising them
CA2947771A1 (en) 2014-06-18 2015-12-23 President And Fellows Of Harvard College Optogenetic probes for measuring membrane potential
CN104357045B (zh) * 2014-11-05 2016-06-01 北京化工大学 具有极酸/极碱开关响应的螺吡喃小分子荧光探针的合成方法及其应用
WO2016115333A1 (en) 2015-01-15 2016-07-21 President And Fellows Of Harvard College Optical selection of cells
US11536716B2 (en) * 2015-06-25 2022-12-27 Hoffmann-La Roche Inc. Cell based assay for determining antibody or ligand binding and function
EP3589639A4 (de) 2017-03-02 2021-03-17 Akita Innovations LLC Spannungssensitive farbstoffe
WO2019212946A1 (en) 2018-04-30 2019-11-07 Ribon Therapeutics Inc. Screening methods for parp modulators
WO2020018700A1 (en) 2018-07-18 2020-01-23 Manzanita Pharmaceuticals, Inc. Conjugates for delivering an anti-cancer agent to nerve cells, methods of use and methods of making thereof
RU197959U1 (ru) * 2020-01-28 2020-06-09 Федеральное государственное бюджетное образовательное учреждение высшего образования "МИРЭА - Российский технологический университет" Устройство для исследования компонент вещества с помощью микроволновой плазмы методами оптической и ямр спектроскопии
WO2022008720A1 (en) * 2020-07-10 2022-01-13 Life & Brain Gmbh Novel hybrid optical voltage sensors

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1597482B2 (de) * 1967-09-22 1977-03-24 Agfa-Gevaert Ag, 5090 Leverkusen Lichtempfindliches photographisches aufzeichnungsmaterial mit lichthofschutz- oder filterfarbstoffen
JPS59218956A (ja) * 1983-05-27 1984-12-10 Fujirebio Inc 膜融合測定方法
DK487784A (da) * 1983-10-13 1985-04-14 Univ Georgia Immunoassay
US4647531A (en) * 1984-02-06 1987-03-03 Ortho Diagnostic Systems, Inc. Generalized cytometry instrument and methods of use
US4861727A (en) * 1986-09-08 1989-08-29 C. R. Bard, Inc. Luminescent oxygen sensor based on a lanthanide complex
US4900934A (en) * 1987-07-15 1990-02-13 University Of Utah Apparatus for simultaneous visualization and measurement of fluorescence from fluorescent dye-treated cell preparations and solutions
AT393035B (de) * 1989-04-25 1991-07-25 Avl Verbrennungskraft Messtech Verfahren zur quantitativen bestimmung zumindest eines chemischen parameters eines probenmediums
US5169788A (en) * 1989-06-09 1992-12-08 New England Deaconess Hospital Corporation Methods of measuring membrane potential using j-aggregate forming dyes
ATE106566T1 (de) * 1989-11-21 1994-06-15 Bayer Ag Optischer biosensor.
US5244813A (en) * 1991-01-25 1993-09-14 Trustees Of Tufts College Fiber optic sensor, apparatus, and methods for detecting an organic analyte in a fluid or vapor sample
DE4121426A1 (de) * 1991-06-28 1993-01-14 Basf Ag Chemischer sensor
EP0552107A1 (de) * 1992-01-17 1993-07-21 The University Of Maryland At Baltimore pH und pC02-Messung durch Luminiszenz-Lebensdauer und Energietransfer
WO1995008637A1 (en) * 1993-09-21 1995-03-30 Washington State University Research Foundation Immunoassay comprising ligand-conjugated, ion channel receptor immobilized in lipid film
GB9406464D0 (en) * 1994-03-31 1994-05-25 British Tech Group Assay for determining biochemical changes at phospholipid bilayer membrane surfaces
US5661035A (en) * 1995-06-07 1997-08-26 The Regents Of The University Of California Voltage sensing by fluorescence resonance energy transfer

Also Published As

Publication number Publication date
EP0977035B2 (de) 2010-07-21
HK1090422A1 (en) 2006-12-22
US6107066A (en) 2000-08-22
JP4033899B2 (ja) 2008-01-16
DE69605041D1 (de) 1999-12-09
EP1610126A1 (de) 2005-12-28
EP0834074A1 (de) 1998-04-08
JPH11508355A (ja) 1999-07-21
DE69605041T2 (de) 2000-03-16
EP0977035B1 (de) 2005-09-14
EP0834074B1 (de) 1999-11-03
WO1996041166A3 (en) 1997-05-15
DE69605041T3 (de) 2006-09-21
ATE186400T1 (de) 1999-11-15
DE69635193T2 (de) 2006-01-19
EP0834074B2 (de) 2005-11-16
ATE304706T1 (de) 2005-09-15
EP0977035A2 (de) 2000-02-02
ES2140870T5 (es) 2006-04-16
US5661035A (en) 1997-08-26
JP4064945B2 (ja) 2008-03-19
CA2223927C (en) 2004-04-06
DK0834074T3 (da) 2000-04-03
AU6264396A (en) 1996-12-30
DE69635193T3 (de) 2011-03-10
CA2223927A1 (en) 1996-12-19
JP2004231666A (ja) 2004-08-19
ES2140870T3 (es) 2000-03-01
EP1610126B1 (de) 2014-04-30
WO1996041166A2 (en) 1996-12-19
EP0977035A3 (de) 2000-03-01
AU716130B2 (en) 2000-02-17
DK0834074T4 (da) 2005-12-12

Similar Documents

Publication Publication Date Title
ATE304706T1 (de) System zur messung des elektrischen potentials durch eine membrane
ATE317122T1 (de) Fluoreszierende polarisation
DE60002562D1 (de) Ein system für zellbasierte reihenuntersuchungen
ATE544747T1 (de) Erkennung von transmembranpotentialen durch optische verfahren
DE69840146D1 (de) Fluoreszenzprotein-sensoren zur erkennung von analyten
WO2002044720A3 (en) Membrane molecule indicator compositions and methods
ES2128321T3 (es) Ensayos de fibrinogeno que usan reactivos quimicos que contienen particulas magneticas.
DK0723146T3 (da) Opkonverterende reportermolekyler til biologiske og andre assays ved anvendelse af laser-excitationsteknikker
WO2000058715A3 (en) Assay system
ES2099626T3 (es) Procedimiento de activacion de colorantes fluorescentes polianionicos en medios de bajo coeficiente dielectrico con compuestos de onio cuaternarios.
Yeung Chemical analysis of single human erythrocytes
WO2001071350A3 (en) Methods of registering trans-membrane electric potentials
ES521810A0 (es) Un metodo para identificar y numerar celulas microbianas en una muestra de material desconocido.
PT696642E (pt) Processo para a identificacao de pertuebacoes do sistema proteina c / proteina s
KR910010187A (ko) σ-히드록시 히푸르산 측정에 의한 맹장염 탐지방법
US4824778A (en) Immunoassay for measuring the concentration of antigen in a sample
BR9300639A (pt) Particulas marcadoras para uso no ensaio de uma amostra de um fluido biologico,processo pra ensaiar uma amostra de fluido biologico,e,processo para ensaiar uma amostra de sangue
EP1421206A4 (de) Verbessertes verfahren zur membranpotentialmessung
Gao et al. High‐throughput determination of glutathione and reactive oxygen species in single cells based on fluorescence images in a microchannel
ES2155084T3 (es) Analisis de la toxicidad.
MX9709597A (es) DTECCIaN DE POTENCIALES DE TRANSMEMBRANA MEDIANTE MÉTODOS aPTICOS.
FR2805545B1 (fr) Procede electrochimique de detection d'acides nucleiques
KR960018583A (ko) 면역 분석법
ATE266856T1 (de) Lichterzeugung, lichtmessung und bestimmung der menge von substanzen in vertiefungen von mikroplatten
Kamentsky Neurophysiology

Legal Events

Date Code Title Description
8363 Opposition against the patent
8366 Restricted maintained after opposition proceedings