ES2132352T3 - Un reactivo para medir leucocitos inmaduros. - Google Patents
Un reactivo para medir leucocitos inmaduros.Info
- Publication number
- ES2132352T3 ES2132352T3 ES94400580T ES94400580T ES2132352T3 ES 2132352 T3 ES2132352 T3 ES 2132352T3 ES 94400580 T ES94400580 T ES 94400580T ES 94400580 T ES94400580 T ES 94400580T ES 2132352 T3 ES2132352 T3 ES 2132352T3
- Authority
- ES
- Spain
- Prior art keywords
- band
- reagent
- immature leukocytes
- suffered
- membranes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 210000000265 leukocyte Anatomy 0.000 title abstract 3
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 2
- 210000000170 cell membrane Anatomy 0.000 abstract 2
- 210000000805 cytoplasm Anatomy 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- -1 POLYOXYETHYLENE Polymers 0.000 abstract 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 abstract 1
- 239000012736 aqueous medium Substances 0.000 abstract 1
- 210000000601 blood cell Anatomy 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 239000002736 nonionic surfactant Substances 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
- G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/825—Pretreatment for removal of interfering factors from sample
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/2525—Stabilizing or preserving
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Dispersion Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Ecology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
SE PRESENTA UN REAGENTE PARA MEDIR LEUCOCITOS INMADUROS QUE COMPRENDE: 1) UN SURFACTANTE NO IONICO BASADO EN POLIOXIETILENO QUE TENGA LA FORMULA GENERAL (I) EN UNA CANTIDAD SUFICIENTE CAPAZ DE FIJAR LAS MEMBRANAS DE CELULAS Y CITOPLASMAS DE LEUCOCITOS INMADUROS; EN DONDE R1 ES UN GRUPO DE ALQUILO, ALQUENILO O ALQUINILO QUE TIENE ENTRE 10 Y 25 ATOMOS DE CARBONO; R2 ES -O-, -(C6H4)-OUN AGENTE SOLUBILIZANTE EN CANTIDAD SUFICIENTE PARA QUE SEA CAPAZ DE DAÑAR LAS MEMBRANAS CELULARES DE LAS CELULAS SANGUINEAS DIFERENTES A LAS DE LOS LEUCOCITOS INMADUROS Y RASGARLAS, 3) UN AMINOACIDO EN UNA CANTIDAD SUFICIENTE CAPAZ DE ESTABILIZAR EL CITOPLASMA Y LA MEMBRANA CELULAR DE LOS LEUCOCITOS INMADUROS, Y 4) UN MEDIO ACUOSO, QUE AJUSTE EL VALOR DE PH EN LA BANDA DE ENTRE 5.0 Y 9.0, LA OSMOLARIDAD EN LA BANDA DE ENTRE 150 Y 600 MOSM/KG Y UNA CONDUCTIVIDAD ELECTRICA Y LA BANDA ENTRE 6.0 Y 9.0 MS/CM, RESPECTIVAMENTE.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP06003793A JP3301646B2 (ja) | 1993-03-19 | 1993-03-19 | 幼若細胞測定用試薬 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2132352T3 true ES2132352T3 (es) | 1999-08-16 |
Family
ID=13130481
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES94400580T Expired - Lifetime ES2132352T3 (es) | 1993-03-19 | 1994-03-17 | Un reactivo para medir leucocitos inmaduros. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5413938A (es) |
EP (1) | EP0617281B1 (es) |
JP (1) | JP3301646B2 (es) |
KR (1) | KR100287583B1 (es) |
CN (1) | CN1088194C (es) |
AU (1) | AU665508B2 (es) |
CA (1) | CA2119390C (es) |
DE (1) | DE69419053T2 (es) |
ES (1) | ES2132352T3 (es) |
TW (1) | TW297093B (es) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3467310B2 (ja) * | 1994-04-21 | 2003-11-17 | シスメックス株式会社 | 白血球分析用試薬及び白血球の分類方法 |
JP3324050B2 (ja) * | 1994-10-31 | 2002-09-17 | 日本光電工業株式会社 | 白血球分類用試薬および白血球分類方法 |
US5691204A (en) * | 1995-04-21 | 1997-11-25 | Abbott Laboratories | Compositions and methods for the rapid analysis of reticulocytes |
FR2735579B1 (fr) * | 1995-06-13 | 1997-09-19 | Hycel Groupe Lisabio | Procede de discrimination et d'isolement de sous-populations de leucocytes a partir d'echantillons sanguins par traitement par du polyoxyethylene 9-lauryl ether, et reactif pour sa mise en oeuvre |
JP4042925B2 (ja) * | 1996-11-20 | 2008-02-06 | シスメックス株式会社 | 幼若白血球の分類計数法 |
US5830701A (en) * | 1997-03-28 | 1998-11-03 | Tao Medical Electronics Co., Ltd. | Method of detecting hematopoietic progenitor cells |
JP3783808B2 (ja) * | 1997-05-19 | 2006-06-07 | シスメックス株式会社 | 白血球分類計数用試薬 |
KR19980042627A (ko) * | 1997-11-20 | 1998-08-17 | 이에츠구히사시 | 유약백혈구의 분류계수법 |
US6225124B1 (en) * | 1999-06-02 | 2001-05-01 | Sysmex Corporation | Diluting reagent and method compelling time-independent consistency in MCV assay |
US6900023B1 (en) | 1999-09-02 | 2005-05-31 | Sysmex Corporation | Method for classifying and counting leukocytes |
US6916658B2 (en) * | 2001-07-27 | 2005-07-12 | Beckman Coulter, Inc. | Method for measurement of immature granulocytes |
JP4227019B2 (ja) * | 2001-10-23 | 2009-02-18 | デンカ生研株式会社 | 血液中の血球と菌の分離方法および血液中の菌の検出方法 |
JP4212827B2 (ja) * | 2002-05-16 | 2009-01-21 | シスメックス株式会社 | 骨髄液有核細胞自動分析方法 |
AU2003243945A1 (en) | 2002-06-24 | 2004-01-06 | Sysmex Corporation | Method of classifying and counting leucocytes |
JP4118618B2 (ja) * | 2002-07-01 | 2008-07-16 | シスメックス株式会社 | 試料分析装置 |
US20040166540A1 (en) * | 2003-02-24 | 2004-08-26 | Sysmex Corporation | Methods of detecting CD34 positive and negative hematopoietic stem cells in human samples |
US7678578B2 (en) | 2005-02-07 | 2010-03-16 | Beckman Coulter, Inc. | Cell permeabilization and stabilization reagent and method of use |
US7541190B2 (en) * | 2005-02-07 | 2009-06-02 | Beckman Coulter, Inc. | Method of measurement of cellular hemoglobin |
JP4806342B2 (ja) * | 2006-01-27 | 2011-11-02 | シスメックス株式会社 | 幼若白血球分析用試薬及び試薬キット |
US7625757B2 (en) * | 2006-01-27 | 2009-12-01 | Sysmex Corporation | Reagent for immature leukocyte analysis and reagent kit |
JP4976038B2 (ja) * | 2006-03-29 | 2012-07-18 | シスメックス株式会社 | 血液学的試料の測定方法 |
JP4806334B2 (ja) * | 2006-11-27 | 2011-11-02 | シスメックス株式会社 | 血液学的試料の測定方法および測定装置 |
EP2166354B1 (en) * | 2007-06-25 | 2012-11-07 | Sysmex Corporation | Reagent and reagent kit for analysis of primitive leukocyte |
RU2435164C2 (ru) | 2007-06-25 | 2011-11-27 | Сисмекс Корпорейшн | Реактив и набор реактивов для анализа незрелых лейкоцитов |
CN101349644B (zh) * | 2007-07-20 | 2012-06-27 | 深圳迈瑞生物医疗电子股份有限公司 | 一种白细胞分类试剂和其使用方法 |
CN101475754A (zh) * | 2008-01-04 | 2009-07-08 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类荧光染料,组合物及在生物样品染色中的用途 |
US20110027826A1 (en) * | 2008-05-02 | 2011-02-03 | Arkray, Inc. | Leukocyte analysis method and analysis reagent for use in the method |
CN101602762B (zh) * | 2008-06-10 | 2013-10-16 | 深圳迈瑞生物医疗电子股份有限公司 | 不对称菁类化合物、其制备方法及应用 |
CN101726579B (zh) * | 2008-10-17 | 2014-06-18 | 深圳迈瑞生物医疗电子股份有限公司 | 血液检测试剂和方法 |
CN101723874B (zh) * | 2008-10-31 | 2013-09-11 | 深圳迈瑞生物医疗电子股份有限公司 | 花菁类化合物及其在生物样品染色中的用途 |
JP5168658B2 (ja) * | 2008-11-21 | 2013-03-21 | 国立大学法人高知大学 | 血球分析装置、血球分析方法及びコンピュータプログラム |
CN101750476B (zh) * | 2008-12-08 | 2015-06-03 | 深圳迈瑞生物医疗电子股份有限公司 | 血液分析试剂及其使用方法 |
CN101750274B (zh) * | 2008-12-17 | 2014-06-25 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒以及白细胞分类计数的方法 |
CN101988082B (zh) * | 2009-07-31 | 2015-04-08 | 深圳迈瑞生物医疗电子股份有限公司 | 白细胞分类计数试剂、试剂盒及其制备方法和白细胞分类计数的方法 |
JP5416232B2 (ja) * | 2012-01-23 | 2014-02-12 | シスメックス株式会社 | 血液学的試料の測定装置 |
US20140377374A1 (en) * | 2012-02-08 | 2014-12-25 | University Of Florida Research Foundation, Inc. | Materials and methods for treating diarrhea |
JP6319959B2 (ja) * | 2013-07-05 | 2018-05-09 | 国立大学法人信州大学 | 白血球数及び左方移動を指標とした細菌感染症の検出 |
CN106687810B (zh) * | 2014-12-31 | 2019-10-22 | 深圳迈瑞生物医疗电子股份有限公司 | 一种非诊断目的的有核红细胞报警方法、装置及流式细胞分析仪 |
KR101849698B1 (ko) * | 2017-08-24 | 2018-04-18 | 한국화학연구원 | 생체 조직 크기 조절용 조성물 및 상기 조성물을 이용한 생체 조직의 크기 조절 방법 |
KR20190094509A (ko) * | 2018-02-05 | 2019-08-14 | 한국화학연구원 | 스페로이드 투명화용 조성물, 이를 이용한 스페로이드 투명화 방법 및 이를 포함하는 키트 |
CN111024563A (zh) * | 2019-12-30 | 2020-04-17 | 深圳开立生物医疗科技股份有限公司 | 一种白细胞分类试剂 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4099917A (en) | 1977-07-21 | 1978-07-11 | Technicon Instruments Corporation | Process for preparing a cell suspension from blood for discrimination of white blood cells and platelets from other blood particles |
JPS5743364A (en) | 1980-06-27 | 1982-03-11 | Union Carbide Corp | Non-aquous battery |
US4751179A (en) | 1984-05-31 | 1988-06-14 | Coulter Electronics, Inc. | Method and reagents for differential determination of four populations of leukocytes in blood |
JP2935529B2 (ja) * | 1990-03-01 | 1999-08-16 | シスメックス株式会社 | 白血球分類方法および試薬 |
AU613642B2 (en) | 1987-05-29 | 1991-08-08 | Toa Medical Electronics Co., Ltd. | Method for classifying leukocytes and reagents |
JP2778745B2 (ja) * | 1989-06-19 | 1998-07-23 | 東亜医用電子株式会社 | 血液中の白血球弁別測定用試薬 |
JP2836865B2 (ja) * | 1989-10-23 | 1998-12-14 | 東亜医用電子株式会社 | 血液中の白血球およびヘモグロビンの測定用試薬 |
US5242832A (en) * | 1990-03-01 | 1993-09-07 | Toa Medical Electronics Co., Ltd. | Reagent for measurement of leukocytes and hemoglobin in blood |
DE4018502A1 (de) * | 1990-06-09 | 1991-12-12 | Merck Patent Gmbh | Verfahren und mittel zur beseitigung von truebungen in biologischen fluessigkeiten |
CA2048302A1 (en) * | 1990-08-15 | 1992-02-16 | Victoria P. Meucci | Solubilization reagent for biological test samples |
DE69327775T2 (de) * | 1992-11-19 | 2000-06-21 | Sysmex Corp | Verfahren zur Vorbehandlung für Blutanalyse |
-
1993
- 1993-03-19 JP JP06003793A patent/JP3301646B2/ja not_active Expired - Lifetime
- 1993-12-28 TW TW082111061A patent/TW297093B/zh not_active IP Right Cessation
-
1994
- 1994-03-17 ES ES94400580T patent/ES2132352T3/es not_active Expired - Lifetime
- 1994-03-17 US US08/214,248 patent/US5413938A/en not_active Expired - Lifetime
- 1994-03-17 DE DE69419053T patent/DE69419053T2/de not_active Expired - Lifetime
- 1994-03-17 EP EP94400580A patent/EP0617281B1/en not_active Expired - Lifetime
- 1994-03-17 AU AU57856/94A patent/AU665508B2/en not_active Expired
- 1994-03-18 CA CA002119390A patent/CA2119390C/en not_active Expired - Lifetime
- 1994-03-19 KR KR1019940005602A patent/KR100287583B1/ko not_active IP Right Cessation
- 1994-03-19 CN CN94102901A patent/CN1088194C/zh not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
KR100287583B1 (ko) | 2001-05-02 |
EP0617281A2 (en) | 1994-09-28 |
AU5785694A (en) | 1994-09-22 |
JP3301646B2 (ja) | 2002-07-15 |
CA2119390A1 (en) | 1994-09-20 |
JPH06273413A (ja) | 1994-09-30 |
DE69419053D1 (de) | 1999-07-22 |
EP0617281B1 (en) | 1999-06-16 |
AU665508B2 (en) | 1996-01-04 |
TW297093B (es) | 1997-02-01 |
CN1095481A (zh) | 1994-11-23 |
CA2119390C (en) | 1997-04-08 |
DE69419053T2 (de) | 1999-10-21 |
EP0617281A3 (en) | 1995-09-06 |
CN1088194C (zh) | 2002-07-24 |
KR940022088A (ko) | 1994-10-20 |
US5413938A (en) | 1995-05-09 |
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