ES2111600T3 - Composiciones reactivas y su uso en la identificacion y caracterizacion de reticulocitos en sangre entera. - Google Patents

Composiciones reactivas y su uso en la identificacion y caracterizacion de reticulocitos en sangre entera.

Info

Publication number
ES2111600T3
ES2111600T3 ES92120309T ES92120309T ES2111600T3 ES 2111600 T3 ES2111600 T3 ES 2111600T3 ES 92120309 T ES92120309 T ES 92120309T ES 92120309 T ES92120309 T ES 92120309T ES 2111600 T3 ES2111600 T3 ES 2111600T3
Authority
ES
Spain
Prior art keywords
cell
reticulocitos
reticulocytes
acridine
characterization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92120309T
Other languages
English (en)
Inventor
Sophie S Fan
Daniel Ben-David
Gregory M Collela
Albert Cupo
Gena Fischer
Leonard Ornstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer AG
Bayer Corp
Icahn School of Medicine at Mount Sinai
Original Assignee
Bayer AG
Bayer Corp
Mount Sinai School of Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer AG, Bayer Corp, Mount Sinai School of Medicine filed Critical Bayer AG
Application granted granted Critical
Publication of ES2111600T3 publication Critical patent/ES2111600T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5094—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for blood cell populations
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012—Red blood cells
    • G01N2015/014—Reticulocytes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1486—Counting the particles
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/101666—Particle count or volume standard or control [e.g., platelet count standards, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Ecology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

METODOS PARA CARACTERIZAR E IDENTIFICAR RETICULOCITOS Y HEMATIES UTILIZAN COMPOSICIONES REACTIVAS QUE INCLUYEN TINTES CATIONICOS ORGANICOS PARA TEÑIR LOS RETICULOCITOS DE LAS MUESTRAS SANGUINEAS Y SOLUCIONES AMORTIGUADORAS PARA MANTENER UN PH ENTRE 6 A 9. LOS TINTES PUEDEN SER TINTE FLUORESCENTE EXCITABLE ROJO OXAZINA 750 O LOS TINTES FLUORESCENTES EXCITABLES AZULES NARANJA DE ACRIDINA O DERIVADOS DE NARANJA DE ACRIDINA. CUANDO UN SUPERFICIACTIVO ZWITERONICO SE INCLUYE EN LA COMPOSICION REACTIVA PARA HACER ESFERICOS ISOVOLUMETRICAMENTE LOS RETICULOCITOS Y ERITROCITOS, Y LA COMPOSICION REACTIVA Y TODA LA MUESTRA DE SANGRE SE PASA POR LA REGION SENSIBLE DE UN CITOMETRO DE FLUJO, SE MIDE LA LUZ ESPARCIDA POR AL MENOS UN INTERVALO ANGULAR Y EL FLUORESCIDO POR CADA CELULA; LOS ERITROCITOS PUEDEN DISTINGUIRSE DE LOS RETICULOCITOS, Y EL VOLUMEN Y CONCENTRACION DE HEMOGLOBINA Y EL CONTENIDO DE HEMOGLOBINA DE CADA RETICULOCITO Y ERITROCITO, Y EL VOLUMEN CELULAR MEDIO, CONCENTRACION DE HEMOGLOBINA CORPUSCULAR MEDIA Y HEMOGLOBINA CELULAR MEDIA DE LOS RETICULOCITOS Y ERITROCITOS SE CALCULA DEL VOLUMEN CELULA POR CELULA MEDIDO Y CONCENTRACION DE HEMOGLOBINA.
ES92120309T 1991-12-05 1992-11-27 Composiciones reactivas y su uso en la identificacion y caracterizacion de reticulocitos en sangre entera. Expired - Lifetime ES2111600T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/802,585 US5360739A (en) 1991-12-05 1991-12-05 Methods for the identification and characterization of reticulocytes in whole blood

Publications (1)

Publication Number Publication Date
ES2111600T3 true ES2111600T3 (es) 1998-03-16

Family

ID=25184134

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92120309T Expired - Lifetime ES2111600T3 (es) 1991-12-05 1992-11-27 Composiciones reactivas y su uso en la identificacion y caracterizacion de reticulocitos en sangre entera.

Country Status (12)

Country Link
US (2) US5360739A (es)
EP (1) EP0545315B1 (es)
JP (1) JPH0827277B2 (es)
KR (1) KR100258394B1 (es)
AT (1) ATE161953T1 (es)
AU (1) AU2816792A (es)
CA (1) CA2077789C (es)
DE (1) DE69223931T2 (es)
DK (1) DK0545315T3 (es)
ES (1) ES2111600T3 (es)
IL (1) IL103055A (es)
TW (1) TW316948B (es)

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Also Published As

Publication number Publication date
JPH0827277B2 (ja) 1996-03-21
DE69223931D1 (de) 1998-02-12
TW316948B (es) 1997-10-01
CA2077789A1 (en) 1993-06-06
US5411891A (en) 1995-05-02
JPH06180315A (ja) 1994-06-28
US5360739A (en) 1994-11-01
AU2816792A (en) 1993-06-10
DK0545315T3 (da) 1998-09-07
CA2077789C (en) 1997-09-09
EP0545315A1 (en) 1993-06-09
KR100258394B1 (ko) 2000-06-01
ATE161953T1 (de) 1998-01-15
IL103055A0 (en) 1993-02-21
IL103055A (en) 1997-07-13
EP0545315B1 (en) 1998-01-07
DE69223931T2 (de) 1998-04-30

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