ES2077151T3 - Penicilina g-acilasa, gen codificador de la misma y procedimiento de produccion de esta enzima. - Google Patents

Penicilina g-acilasa, gen codificador de la misma y procedimiento de produccion de esta enzima.

Info

Publication number
ES2077151T3
ES2077151T3 ES91200929T ES91200929T ES2077151T3 ES 2077151 T3 ES2077151 T3 ES 2077151T3 ES 91200929 T ES91200929 T ES 91200929T ES 91200929 T ES91200929 T ES 91200929T ES 2077151 T3 ES2077151 T3 ES 2077151T3
Authority
ES
Spain
Prior art keywords
gene
enzyme
same
penicillin
acilase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91200929T
Other languages
English (en)
Inventor
Wilhelmus Johannes Quax
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Application granted granted Critical
Publication of ES2077151T3 publication Critical patent/ES2077151T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/84—Penicillin amidase (3.5.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01011—Penicillin amidase (3.5.1.11), i.e. penicillin-amidohydrolase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/829—Alcaligenes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

LA INVENCION SUMINISTRA UN GEN CODIFICANDO LA PENICILINA G ACILASA, LA ENZINA CODIFICADA POR DICHO GEN Y UN METODO PARA LA PRODUCCION DE DICHA ENZIMA MEDIANTE LA INCORPORACION DE DICHO GEN EN UN RECEPTOR Y LLEVANDOLO A EXPRESION. EL GEN ES PREFERIBLEMENTE OBTENIDO DE UN ESFUERZO DEL MICROORGANISMO ALCALIGENES FALCALIS.
ES91200929T 1990-04-18 1991-04-18 Penicilina g-acilasa, gen codificador de la misma y procedimiento de produccion de esta enzima. Expired - Lifetime ES2077151T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP90200962 1990-04-18
EP90203463 1990-12-20

Publications (1)

Publication Number Publication Date
ES2077151T3 true ES2077151T3 (es) 1995-11-16

Family

ID=26125836

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91200929T Expired - Lifetime ES2077151T3 (es) 1990-04-18 1991-04-18 Penicilina g-acilasa, gen codificador de la misma y procedimiento de produccion de esta enzima.

Country Status (10)

Country Link
US (2) US5168048A (es)
EP (1) EP0453047B1 (es)
JP (1) JPH04228073A (es)
KR (1) KR0152667B1 (es)
AT (1) ATE124724T1 (es)
DE (1) DE69110952T2 (es)
DK (1) DK0453047T3 (es)
ES (1) ES2077151T3 (es)
IE (1) IE68078B1 (es)
PT (1) PT97397B (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE167520T1 (de) * 1990-04-18 1998-07-15 Gist Brocades Nv Mutierte beta-lactamacylasegene
DE69421653D1 (de) * 1993-08-05 1999-12-23 Dsm Nv Neue Anwendung von Alcaligenes faecalis Penicillin G Acylase
US5891703A (en) * 1994-08-12 1999-04-06 Gist-Hrocades Mutated penicillin G acylase genes
US5516679A (en) * 1994-12-23 1996-05-14 Bristol-Myers Squibb Company Penicillin V amidohydrolase gene from Fusarium oxysporum
EP0863989A2 (en) * 1995-11-27 1998-09-16 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
US6403356B1 (en) 1996-11-05 2002-06-11 Bristol-Myers Squibb Co. Mutant penicillin G acylases
US6437113B1 (en) * 1998-03-05 2002-08-20 Tularik Inc. Suppressors of death domains
WO2000066751A1 (en) * 1999-04-29 2000-11-09 Dsm N.V. Expression cassette for efficient production of a protein
US20030044884A1 (en) * 2000-10-11 2003-03-06 Usher John J. Synthesis of beta-lactam antibacterials using soluble side chain esters and enzyme acylase
US6699699B2 (en) 2002-03-26 2004-03-02 Synmax Biochemical Co., Ltd. Mutated penicillin expandases
AU2003241946A1 (en) 2002-06-06 2003-12-22 Kaneka Corporation Novel acylase gene
KR100530299B1 (ko) * 2003-08-11 2005-11-22 산도즈 게엠베하 변이 세팔로스포린 ｃ 아실라제 및 이를 이용한 7-ａｃａ 제조방법
ITMI20040016A1 (it) * 2004-01-12 2004-04-12 Antibiotics S P A Cefalosporina c acilasi
CZ300467B6 (cs) * 2007-01-31 2009-05-27 Mikrobiologický ústav AV CR, v.v.i. Sekvence nukleotidu o velikosti 2646 bp, kódující penicilinacylázu, nové konstrukty rekombinantní nukleové kyseliny a rekombinantní mikroorganismy nesoucí tuto sekvenci
TW201022445A (en) * 2008-10-09 2010-06-16 Dsm Ip Assets Bv Method for preparing ε-caprolactam from N-acyl-6-aminocaproic acid
CN104981156A (zh) 2012-12-14 2015-10-14 萨班企业私人有限公司 协同消毒增强
CN104981158B (zh) 2012-12-14 2017-12-22 萨班企业私人有限公司 消毒剂
CN113913416B (zh) * 2021-10-14 2022-03-22 巢湖学院 一种粪产碱杆菌青霉素g酰化酶的制备工艺
CN115418331A (zh) * 2022-08-29 2022-12-02 新发药业有限公司 一种产青霉素g酰基转移酶自诱导培养基及其应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3239427A (en) * 1959-10-12 1966-03-08 Pfizer & Co C Production of 6-aminopenicillanic acid
US3272715A (en) * 1962-07-17 1966-09-13 American Home Prod Method for producing and recovering 6-aminopenicillanic acid
BE790075A (fr) * 1971-10-14 1973-04-13 Bayer Ag Procede de preparation de penicillinacylase cristalline pure etproduit obtenu
DE2732301C3 (de) * 1977-07-16 1980-12-11 Roehm Gmbh, 6100 Darmstadt Verfahren zur Herstellung von stabilisierten trägergebundenen Proteinen
DE3330003A1 (de) * 1982-10-21 1984-10-31 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Dna-sequenz und dna-strukturen und sie verwendendes verfahren zur herstellung von penicillin-acylase
JPS6066981A (ja) * 1983-09-12 1985-04-17 アメリカン・ホ−ム・プロダクツ・コ−ポレイシヨン 不溶化ペニシリンアミダ−ゼおよびその製法
ES2054916T3 (es) * 1988-04-08 1994-08-16 Biochemie Gmbh Gen y producto de gen para la obtencion de compuestos de beta-lactama.
DE3909018A1 (de) * 1989-03-18 1990-09-20 Roehm Gmbh Protein-reinigungsverfahren

Also Published As

Publication number Publication date
KR910018550A (ko) 1991-11-30
US5695978A (en) 1997-12-09
ATE124724T1 (de) 1995-07-15
DE69110952T2 (de) 1995-11-16
DK0453047T3 (da) 1995-09-11
PT97397B (pt) 1998-08-31
EP0453047A1 (en) 1991-10-23
KR0152667B1 (ko) 1998-10-01
IE68078B1 (en) 1996-05-15
PT97397A (pt) 1992-01-31
DE69110952D1 (de) 1995-08-10
EP0453047B1 (en) 1995-07-05
JPH04228073A (ja) 1992-08-18
IE911294A1 (en) 1991-10-23
US5168048A (en) 1992-12-01

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