DE370958T1 - Heparinase produzierender mikroorganismus des stammes bacillus, neue heparinase und verfahren zu ihrer herstellung. - Google Patents
Heparinase produzierender mikroorganismus des stammes bacillus, neue heparinase und verfahren zu ihrer herstellung.Info
- Publication number
- DE370958T1 DE370958T1 DE198989810894T DE89810894T DE370958T1 DE 370958 T1 DE370958 T1 DE 370958T1 DE 198989810894 T DE198989810894 T DE 198989810894T DE 89810894 T DE89810894 T DE 89810894T DE 370958 T1 DE370958 T1 DE 370958T1
- Authority
- DE
- Germany
- Prior art keywords
- positive
- negative
- heparinase
- growth
- microorganism
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108010022901 Heparin Lyase Proteins 0.000 title claims abstract 9
- 244000005700 microbiome Species 0.000 title claims abstract 8
- 238000004519 manufacturing process Methods 0.000 title claims abstract 5
- 238000000034 method Methods 0.000 title claims 5
- 241000193830 Bacillus <bacterium> Species 0.000 title claims 3
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 claims abstract 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 10
- 230000012010 growth Effects 0.000 claims 8
- 239000011780 sodium chloride Substances 0.000 claims 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 235000015097 nutrients Nutrition 0.000 claims 3
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 claims 2
- 229920002567 Chondroitin Polymers 0.000 claims 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims 2
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims 2
- DLGJWSVWTWEWBJ-HGGSSLSASA-N chondroitin Chemical compound CC(O)=N[C@@H]1[C@H](O)O[C@H](CO)[C@H](O)[C@@H]1OC1[C@H](O)[C@H](O)C=C(C(O)=O)O1 DLGJWSVWTWEWBJ-HGGSSLSASA-N 0.000 claims 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims 2
- RXKJFZQQPQGTFL-UHFFFAOYSA-N dihydroxyacetone Chemical compound OCC(=O)CO RXKJFZQQPQGTFL-UHFFFAOYSA-N 0.000 claims 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 claims 2
- 229920000669 heparin Polymers 0.000 claims 2
- 229960002897 heparin Drugs 0.000 claims 2
- 239000002609 medium Substances 0.000 claims 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims 1
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims 1
- CDVZCUKHEYPEQS-KPXKHRLBSA-N (2s,3r,4r)-2,3,4,5-tetrahydroxypentanal;(2r,3s,4s)-2,3,4,5-tetrahydroxypentanal Chemical compound OC[C@H](O)[C@H](O)[C@@H](O)C=O.OC[C@@H](O)[C@@H](O)[C@H](O)C=O CDVZCUKHEYPEQS-KPXKHRLBSA-N 0.000 claims 1
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims 1
- 102000016938 Catalase Human genes 0.000 claims 1
- 108010053835 Catalase Proteins 0.000 claims 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims 1
- RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 claims 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims 1
- 229920001353 Dextrin Polymers 0.000 claims 1
- 239000004375 Dextrin Substances 0.000 claims 1
- 239000004386 Erythritol Substances 0.000 claims 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims 1
- 102000016943 Muramidase Human genes 0.000 claims 1
- 108010014251 Muramidase Proteins 0.000 claims 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims 1
- 229910002651 NO3 Inorganic materials 0.000 claims 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims 1
- QMYDHJSXOZCPFF-WGGIRBBPSA-N OC[C@@H](O)[C@H](O)[C@@H](O)C=O.C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O Chemical compound OC[C@@H](O)[C@H](O)[C@@H](O)C=O.C[C@H](O)[C@H](O)[C@@H](O)[C@@H](O)C=O QMYDHJSXOZCPFF-WGGIRBBPSA-N 0.000 claims 1
- 229920002230 Pectic acid Polymers 0.000 claims 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 claims 1
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 claims 1
- 229920002472 Starch Polymers 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 claims 1
- 238000009651 Voges-Proskauer test Methods 0.000 claims 1
- 230000009603 aerobic growth Effects 0.000 claims 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims 1
- 230000009604 anaerobic growth Effects 0.000 claims 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims 1
- 150000001540 azides Chemical class 0.000 claims 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims 1
- 229910001424 calcium ion Inorganic materials 0.000 claims 1
- 150000001720 carbohydrates Chemical class 0.000 claims 1
- 235000014633 carbohydrates Nutrition 0.000 claims 1
- 239000005018 casein Substances 0.000 claims 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims 1
- 235000021240 caseins Nutrition 0.000 claims 1
- 230000015556 catabolic process Effects 0.000 claims 1
- 239000013078 crystal Substances 0.000 claims 1
- 229940104302 cytosine Drugs 0.000 claims 1
- 238000000354 decomposition reaction Methods 0.000 claims 1
- 238000006731 degradation reaction Methods 0.000 claims 1
- 235000019425 dextrin Nutrition 0.000 claims 1
- 229940120503 dihydroxyacetone Drugs 0.000 claims 1
- 229940009714 erythritol Drugs 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 239000003102 growth factor Substances 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 229920002674 hyaluronan Polymers 0.000 claims 1
- 229960003160 hyaluronic acid Drugs 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 claims 1
- 238000006460 hydrolysis reaction Methods 0.000 claims 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims 1
- 229960000367 inositol Drugs 0.000 claims 1
- 239000008101 lactose Substances 0.000 claims 1
- 239000004325 lysozyme Substances 0.000 claims 1
- 229960000274 lysozyme Drugs 0.000 claims 1
- 235000010335 lysozyme Nutrition 0.000 claims 1
- 235000010355 mannitol Nutrition 0.000 claims 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims 1
- 230000000877 morphologic effect Effects 0.000 claims 1
- 239000010318 polygalacturonic acid Substances 0.000 claims 1
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 claims 1
- HEBKCHPVOIAQTA-ZXFHETKHSA-N ribitol Chemical compound OC[C@H](O)[C@H](O)[C@H](O)CO HEBKCHPVOIAQTA-ZXFHETKHSA-N 0.000 claims 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims 1
- 239000000600 sorbitol Substances 0.000 claims 1
- 239000008107 starch Substances 0.000 claims 1
- 235000019698 starch Nutrition 0.000 claims 1
- 230000000638 stimulation Effects 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 claims 1
- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Claims (7)
1. Heparinase mit folgenden physikochemischen Eigenschaften:
(1) Optimale Aktivität innerhalb des pH-Bereiches: pH 7.2 /v 7.8
(2) Optimale Aktivität innerhalb des Temperaturbereiches: 45 ~ 50 0C
(3) Anregung der Aktivität durch Ca -Ionen.
2. Eine biologisch reine Kultur eines Mikroorganismus, der im wesentlichen die
charakteristischen Merkmale des Bazillus sp. BHlOO (FERM BP-2613 (FERM
&rgr; 10408)) besitzt, wobei dieser Mikroorganismus die Eigenschaft hat, extrazellulär Heparinase herzustellen.
3. Eine biologisch reine Kultur eines Mikroorganismus, der ein Stamm der
Gattung Bazillus ist und der nachfolgende toxonomische Eigenschaften aufweist:
1. Morphologische Eigenschaften Gramfärbung
Zellgröße
Sporenform
Sporenform
Sporangium, geschwollen Sporenposition
Parasporale Kristalle Beweglichkeit
Parasporale Kristalle Beweglichkeit
2. Wachstumseigenschaften Aerobisches Wachstum Anaerobisches Wachstum
Positiv 0.6-0.8 /im &khgr; 3-6 /im
Zylindrisch Positiv Terminal Negativ Positiv
Positiv Positiv
Maximale Wachstumstemperatur 55 0C
Minimale Wachstumstemperatur 20 0C
Optimale Wachstumstemperatur 45 0C Wachstum in Anwesenheit von Lysozym (0.001 %) Positiv
Wachstum in Anwesenheit von Azid (0.02 %) Negative
Wachstum mit 2 %igem NaCl Positiv
5 feigem NaCl Negativ
7 %igem NaCl Negativ
10 %igem NaCl Negativ
Wachstum beim pH-Wert 6.8 (Nährflüssigkeit) Positiv pH-Wert 5.7 (Sabouraud Medium) Positiv
Anforderung an NaCl und KCl Negativ
Erforderliche Wachstumsfaktoren keine
3. Biochemische Eigenschaften Katalase
Voges-Proskauer-Test pH in V-P-Nährflüssigkeit Säure von D-Ribose D-Xylose
L-Rhamnose D-Galactose D-Fructose D-Arabinose L-Arabinose Raffinose
D-Mannose Maltose Sucrose Lactose D-Glucose D-Trehalose
Glycerol D-Mannitol
Positiv Negativ 6.5-7.0 Positiv Positiv Positiv Positiv Positiv Positiv
Positiv Positiv Positiv Positiv Positiv Positiv Positiv Positiv Positiv Positiv
4. Verfahren zum Herstellen einer Heparinaseform, dadurch gekennzeichnet, daß
man diese in einem wäßrigen Nährstoffmedium eines Mikroorganismus der Gattung Bazillus, der in der Lage ist, diese Heparinaseform extrazellular
herzustellen, kultiviert, daß man die Heparinase im Kulturmedium anreichert und daß man sie zurückgewinnt.
5. Verfahren nach Anspruch 4, wobei die Heparinaseform Heparinase, wie in
Anspruch 1 spezifiziert, ist.
6. Verfahren nach Anspruch 4 oder Anspruch 5, wobei der Mikroorganismus
einer wie in Anspruch 3 spezifiziert, ist.
7. Verfahren nach einem der Ansprüche 4, 5 oder 6, wobei der verwendete
Mikroorganismus Bazillus sp. BH 100 (FERM BP-2613 (FERM p-10408) ist.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63297807A JPH0693836B2 (ja) | 1988-11-25 | 1988-11-25 | バチルス属に属するヘパリナーゼ生産微生物、新規ヘパリナーゼ及びその製法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE370958T1 true DE370958T1 (de) | 1990-11-08 |
Family
ID=17851424
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE68916915T Expired - Fee Related DE68916915T2 (de) | 1988-11-25 | 1989-11-22 | Heparinase produzierender Mikroorganismus des Stammes Bacillus, neue Heparinase und Verfahren zu ihrer Herstellung. |
| DE198989810894T Pending DE370958T1 (de) | 1988-11-25 | 1989-11-22 | Heparinase produzierender mikroorganismus des stammes bacillus, neue heparinase und verfahren zu ihrer herstellung. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE68916915T Expired - Fee Related DE68916915T2 (de) | 1988-11-25 | 1989-11-22 | Heparinase produzierender Mikroorganismus des Stammes Bacillus, neue Heparinase und Verfahren zu ihrer Herstellung. |
Country Status (5)
| Country | Link |
|---|---|
| US (3) | US5145778A (de) |
| EP (1) | EP0370958B1 (de) |
| JP (1) | JPH0693836B2 (de) |
| AT (1) | ATE108826T1 (de) |
| DE (2) | DE68916915T2 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0693836B2 (ja) * | 1988-11-25 | 1994-11-24 | 新技術事業団 | バチルス属に属するヘパリナーゼ生産微生物、新規ヘパリナーゼ及びその製法 |
| JP3110064B2 (ja) * | 1991-03-06 | 2000-11-20 | 生化学工業株式会社 | 新規ヘパリチナーゼ、その製造法及びその生産菌 |
| US5262325A (en) * | 1991-04-04 | 1993-11-16 | Ibex Technologies, Inc. | Method for the enzymatic neutralization of heparin |
| US5599659A (en) * | 1993-03-11 | 1997-02-04 | Breonics, Inc. | Preservation solution for ex vivo, warm preservation of tissues, explants,organs and vascular endothelial cells comprising retinal-derived fibroblast growth factor, cyclodextrin and chondroitin sulfate |
| US6198958B1 (en) | 1998-06-11 | 2001-03-06 | Beth Israel Deaconess Medical Center, Inc. | Method and apparatus for monitoring a magnetic resonance image during transcranial magnetic stimulation |
| CA2341412A1 (en) * | 1998-08-27 | 2000-03-09 | Massachusetts Institute Of Technology | Rationally designed heparinases derived from heparinase i and ii |
| AU4351201A (en) * | 2000-03-08 | 2001-09-17 | Massachusetts Inst Technology | Heparinase iii and uses thereof |
| AU2003243396A1 (en) * | 2002-06-03 | 2003-12-19 | Massachusetts Institute Of Technology | Rationally designed polysaccharide lyases derived from chondroitinase b |
| US20050277826A1 (en) * | 2004-06-10 | 2005-12-15 | Conopco, Inc. | Apparatus and method for reducing interference |
| US7691613B2 (en) | 2006-11-03 | 2010-04-06 | Momenta Pharmaceuticals, Inc. | Glycosaminoglycan lyase IV and uses thereof |
| US7767420B2 (en) * | 2005-11-03 | 2010-08-03 | Momenta Pharmaceuticals, Inc. | Heparan sulfate glycosaminoglycan lyase and uses thereof |
| US7691612B2 (en) | 2005-11-03 | 2010-04-06 | Momenta Pharmaceuticals, Inc. | Heparan sulfate glycosaminoglycan lyase and uses thereof |
| CN102586217A (zh) * | 2011-12-16 | 2012-07-18 | 深圳市海普瑞药业股份有限公司 | 肝素黄杆菌肝素酶ⅰ、ⅱ、ⅲ的冻干保存方法 |
| CN104593347B (zh) * | 2015-03-05 | 2018-08-28 | 深圳市海普瑞药业集团股份有限公司 | 来自Sphingobacterium daejeonense的肝素酶及其制备和应用 |
| WO2018113775A1 (zh) | 2016-12-22 | 2018-06-28 | 深圳市海普瑞药业集团股份有限公司 | 产肝素酶的施氏假单胞菌菌株及从其衍生的肝素酶 |
| CN113249277B (zh) * | 2021-07-05 | 2021-09-24 | 广东海洋大学 | 一株贝雷斯芽孢杆菌在水产品肝素提取中的应用 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4341869A (en) * | 1980-08-25 | 1982-07-27 | Massachusetts Institute Of Technology | Process for producing heparinase |
| JPH0693836B2 (ja) * | 1988-11-25 | 1994-11-24 | 新技術事業団 | バチルス属に属するヘパリナーゼ生産微生物、新規ヘパリナーゼ及びその製法 |
-
1988
- 1988-11-25 JP JP63297807A patent/JPH0693836B2/ja not_active Expired - Lifetime
-
1989
- 1989-11-22 DE DE68916915T patent/DE68916915T2/de not_active Expired - Fee Related
- 1989-11-22 EP EP89810894A patent/EP0370958B1/de not_active Expired - Lifetime
- 1989-11-22 US US07/440,061 patent/US5145778A/en not_active Expired - Fee Related
- 1989-11-22 DE DE198989810894T patent/DE370958T1/de active Pending
- 1989-11-22 AT AT89810894T patent/ATE108826T1/de not_active IP Right Cessation
-
1991
- 1991-11-27 US US07/799,597 patent/US5362645A/en not_active Expired - Fee Related
-
1994
- 1994-08-03 US US08/285,383 patent/US5455162A/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0370958A2 (de) | 1990-05-30 |
| US5455162A (en) | 1995-10-03 |
| US5362645A (en) | 1994-11-08 |
| JPH02142470A (ja) | 1990-05-31 |
| DE68916915D1 (de) | 1994-08-25 |
| JPH0693836B2 (ja) | 1994-11-24 |
| EP0370958A3 (de) | 1991-05-29 |
| DE68916915T2 (de) | 1994-11-03 |
| ATE108826T1 (de) | 1994-08-15 |
| EP0370958B1 (de) | 1994-07-20 |
| US5145778A (en) | 1992-09-08 |
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