ES2054608T3 - Un metodo para la expresion de genes en levadura y fragmentos de adn asi como plasmidos que comprenden dichos fragmentos de adn para uso cuando se lleva a cabo dicho metodo. - Google Patents
Un metodo para la expresion de genes en levadura y fragmentos de adn asi como plasmidos que comprenden dichos fragmentos de adn para uso cuando se lleva a cabo dicho metodo.Info
- Publication number
- ES2054608T3 ES2054608T3 ES86114704T ES86114704T ES2054608T3 ES 2054608 T3 ES2054608 T3 ES 2054608T3 ES 86114704 T ES86114704 T ES 86114704T ES 86114704 T ES86114704 T ES 86114704T ES 2054608 T3 ES2054608 T3 ES 2054608T3
- Authority
- ES
- Spain
- Prior art keywords
- dna fragments
- carrying
- heme
- plasmids
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
METODO DE EXPRESION DE GENES EN LEVADURA POR INTRODUCCION EN UNA CELULA DE LEVADURA DE UN SEGMENTO RECOMBINANTE DE DNA QUE CONTIENE EL GEN A EXPRESAR Y UNA REGION DE FLANQUEO 5'' QUE COMPRENDE UNA SECUENCIA PROMOTORA, Y CULTIVO DE LAS CELULAS DE LEVADURA TRANSFORMADOS EN UN MEDIO DE CRECIMIENTO, UTILIZANDO COMO SEGMENTO DE RECOMBINACION DE DNA UN SEGMENTO DEL CUAL, LA REGION DE FLANQUEO 5'' COMPRENDE UN PRIMER FRAGMENTO DE DNA CONTENIENDO UNO, SECUENCIA PROMOTORA EN COMBINACION CON UN SEGUNDO FRAGMENTO DE DNA QUE CONTIENE UNA SECUENCIA CONDUCTORA REGULADA SOBRE EL NIVEL POSTTRANSCRIPCIONAL POR EL HEME, ANALOGOS DEL HEME O PRECURSORES DEL HEME. REALIZANDO EL METODO SEGUN LA INVENCION SE OBTIENE UNA EXPRESION AUMENTADA DE GENES POR REDUCCION DE UNA CARGA GENETICA DE LA CELULA HUESPED Y UNA MEJOR UTILIZACION DEL APARATO DE SINTESIS DE PROTEINAS Y EL METABOLISMO ENERGETICO.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK488985A DK162900C (da) | 1985-10-24 | 1985-10-24 | Fremgangsmaade til ekspression af gener i gaer samt dna-fragment, rekombineret dna-segment og plasmider, hvor disse indgaar til brug ved udoevelse af fremgangsmaaden |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2054608T3 true ES2054608T3 (es) | 1994-08-16 |
Family
ID=8137569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES86114704T Expired - Lifetime ES2054608T3 (es) | 1985-10-24 | 1986-10-23 | Un metodo para la expresion de genes en levadura y fragmentos de adn asi como plasmidos que comprenden dichos fragmentos de adn para uso cuando se lleva a cabo dicho metodo. |
Country Status (19)
| Country | Link |
|---|---|
| US (1) | US4849348A (es) |
| EP (1) | EP0220679B1 (es) |
| JP (1) | JPS62181787A (es) |
| AT (2) | ATE90386T1 (es) |
| AU (1) | AU593296B2 (es) |
| BR (1) | BR8605221A (es) |
| CA (1) | CA1333162C (es) |
| DE (2) | DE3636117A1 (es) |
| DK (1) | DK162900C (es) |
| ES (1) | ES2054608T3 (es) |
| FI (1) | FI91083C (es) |
| FR (1) | FR2598432B1 (es) |
| GB (1) | GB2183656B (es) |
| IE (1) | IE59114B1 (es) |
| NL (1) | NL8602657A (es) |
| NO (1) | NO175103C (es) |
| NZ (1) | NZ217948A (es) |
| PT (1) | PT83616B (es) |
| SE (1) | SE8604517L (es) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK162399C (da) * | 1986-01-28 | 1992-03-23 | Danisco | Fremgangsmaade til ekspression af gener i baelgplanteceller, dna-fragment, rekombineret dna-fragment samt plasmid til brug ved udoevelsen af fremgangsmaaden |
| JP2902118B2 (ja) * | 1993-05-24 | 1999-06-07 | ユニバーシティ オブ マサチューセッツ メディカル センター | 微量無機質による転写後の遺伝子の調節 |
| US5824511A (en) * | 1995-08-01 | 1998-10-20 | University Technology Corporation | Method for enhancing the production of hemoproteins |
| US6575703B2 (en) | 2001-07-20 | 2003-06-10 | General Electric Company | Turbine disk side plate |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1341116C (en) * | 1983-02-22 | 2000-10-17 | Rae Lyn Burke | Yeast expression systems with vectors having gapdh or pyk promoters and synthesis or foreign protein |
-
1985
- 1985-10-24 DK DK488985A patent/DK162900C/da not_active IP Right Cessation
-
1986
- 1986-06-13 US US06/874,069 patent/US4849348A/en not_active Expired - Fee Related
- 1986-10-15 NZ NZ217948A patent/NZ217948A/xx unknown
- 1986-10-21 GB GB8625180A patent/GB2183656B/en not_active Expired - Fee Related
- 1986-10-23 AT AT86114704T patent/ATE90386T1/de active
- 1986-10-23 DE DE19863636117 patent/DE3636117A1/de not_active Ceased
- 1986-10-23 NL NL8602657A patent/NL8602657A/nl not_active Application Discontinuation
- 1986-10-23 SE SE8604517A patent/SE8604517L/ not_active Application Discontinuation
- 1986-10-23 NO NO864250A patent/NO175103C/no unknown
- 1986-10-23 ES ES86114704T patent/ES2054608T3/es not_active Expired - Lifetime
- 1986-10-23 IE IE279386A patent/IE59114B1/en not_active IP Right Cessation
- 1986-10-23 EP EP86114704A patent/EP0220679B1/en not_active Expired - Lifetime
- 1986-10-23 DE DE8686114704T patent/DE3688549T2/de not_active Expired - Fee Related
- 1986-10-23 FI FI864301A patent/FI91083C/fi not_active IP Right Cessation
- 1986-10-23 AU AU64338/86A patent/AU593296B2/en not_active Ceased
- 1986-10-23 AT AT0282386A patent/AT396249B/de not_active IP Right Cessation
- 1986-10-24 PT PT83616A patent/PT83616B/pt not_active IP Right Cessation
- 1986-10-24 FR FR868614807A patent/FR2598432B1/fr not_active Expired
- 1986-10-24 JP JP61253530A patent/JPS62181787A/ja active Pending
- 1986-10-24 BR BR8605221A patent/BR8605221A/pt not_active Application Discontinuation
- 1986-10-24 CA CA000521391A patent/CA1333162C/en not_active Expired - Fee Related
Also Published As
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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