ES2026206T3 - Procedimiento para mejorar la expresion de proteina de eucariota. - Google Patents
Procedimiento para mejorar la expresion de proteina de eucariota.Info
- Publication number
- ES2026206T3 ES2026206T3 ES198787900133T ES87900133T ES2026206T3 ES 2026206 T3 ES2026206 T3 ES 2026206T3 ES 198787900133 T ES198787900133 T ES 198787900133T ES 87900133 T ES87900133 T ES 87900133T ES 2026206 T3 ES2026206 T3 ES 2026206T3
- Authority
- ES
- Spain
- Prior art keywords
- protein
- expression
- cdna
- eucariota
- procedure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
- A61B90/03—Automatic limiting or abutting means, e.g. for safety
- A61B2090/033—Abutting means, stops, e.g. abutting on tissue or skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B90/00—Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
- A61B90/06—Measuring instruments not otherwise provided for
- A61B2090/062—Measuring instruments not otherwise provided for penetration depth
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Networks Using Active Elements (AREA)
- Saccharide Compounds (AREA)
Abstract
Procedimiento para la expresión de proteína de eucariota, mejorada con relación al ADNc, en el que el ADNc de un gen de eucariota se clona en el seno de un procariota, el ADNc clonado se obtiene a partir de éste y se introduce el mismo en una célula de eucariota, que traduce el ADNc a la proteína deseada, caracterizado porque se obtiene por lo menos un intrón que se presenta por naturaleza en el correspondiente gen, se incorpora y liga el ADNc en la secuencia codificadora en un lugar apropiado, y se clona en el seno de un procariota el ADNc que contiene intrón, así obtenido.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19853545126 DE3545126A1 (de) | 1985-12-19 | 1985-12-19 | Verfahren zur verbesserung der expression von eukaryontenprotein |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2026206T3 true ES2026206T3 (es) | 1992-04-16 |
Family
ID=6288962
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES198787900133T Expired - Lifetime ES2026206T3 (es) | 1985-12-19 | 1986-12-19 | Procedimiento para mejorar la expresion de proteina de eucariota. |
Country Status (14)
Country | Link |
---|---|
EP (2) | EP0227064A1 (es) |
JP (1) | JPS62502942A (es) |
KR (1) | KR880700855A (es) |
AT (1) | ATE67242T1 (es) |
AU (1) | AU600253B2 (es) |
DE (2) | DE3545126A1 (es) |
DK (1) | DK432987D0 (es) |
ES (1) | ES2026206T3 (es) |
FI (1) | FI873572A0 (es) |
GR (1) | GR3003301T3 (es) |
IL (1) | IL80960A0 (es) |
PT (1) | PT83968B (es) |
WO (1) | WO1987003904A1 (es) |
ZA (1) | ZA869487B (es) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU1187588A (en) * | 1987-01-15 | 1988-08-10 | Codon Genetic Engineering Laboratories | Tandem gene eukaryotic expression vectors |
US5024939A (en) * | 1987-07-09 | 1991-06-18 | Genentech, Inc. | Transient expression system for producing recombinant protein |
DE3730246A1 (de) * | 1987-09-09 | 1989-06-01 | Boehringer Mannheim Gmbh | Expressionsvektor und verfahren zur expression von heterologen proteinen in saeugerzellen |
CA2018676C (en) * | 1989-06-20 | 2003-05-06 | Christie A. Kelton | Novel heteropolymeric protein production methods |
FR2650838A1 (fr) * | 1989-08-09 | 1991-02-15 | Transgene Sa | Vecteurs d'expression du facteur ix dans une cellule eucaryote superieure, procede de preparation de facteur ix par des animaux transgeniques et facteur ix obtenu |
EP0451700A1 (en) * | 1990-04-10 | 1991-10-16 | Miles Inc. | Recombinant APP minigenes for expression in transgenic mice as models for Alzheimers's disease |
EP0530365A1 (en) * | 1991-01-29 | 1993-03-10 | Teijin Limited | Human protein c expression vector |
US5858784A (en) | 1991-12-17 | 1999-01-12 | The Regents Of The University Of California | Expression of cloned genes in the lung by aerosol- and liposome-based delivery |
US6627615B1 (en) | 1991-12-17 | 2003-09-30 | The Regents Of The University Of California | Methods and compositions for in vivo gene therapy |
US5756353A (en) * | 1991-12-17 | 1998-05-26 | The Regents Of The University Of California | Expression of cloned genes in the lung by aerosol-and liposome-based delivery |
US6806084B1 (en) | 1992-06-04 | 2004-10-19 | The Regents Of The University Of California | Methods for compositions for in vivo gene delivery |
WO1993024640A2 (en) * | 1992-06-04 | 1993-12-09 | The Regents Of The University Of California | Methods and compositions for in vivo gene therapy |
WO1997038123A1 (en) * | 1996-04-05 | 1997-10-16 | Board Of Regents, The University Of Texas System | Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells |
EP0816499A3 (en) * | 1996-06-27 | 1999-10-27 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | Genomic DNA encoding a polypeptide capable of inducing the production of interferon-gamma |
JP2002517180A (ja) | 1997-12-05 | 2002-06-18 | ジ・イミユーン・リスポンス・コーポレーシヨン | 増大された発現を示す新規ベクターおよび遺伝子 |
SE9901339D0 (sv) * | 1999-04-15 | 1999-04-15 | Astra Ab | New expression systems |
GB201106380D0 (en) * | 2011-04-15 | 2011-06-01 | Nokia Corp | An apparatus, method, computer program and user interface |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2534273B1 (fr) * | 1982-10-07 | 1985-09-13 | Pasteur Institut | Lignees cellulaires originaires de mammiferes superieurs, productrices d'hormone de croissance, de preference humaine, et vecteurs pour leur obtention |
FR2535243B1 (fr) * | 1982-11-03 | 1986-04-04 | Fives Cail Babcock | Procede de fabrication d'un cylindre, notamment pour moulin a cannes, et cylindre obtenu par ce procede |
IL71691A (en) * | 1984-04-27 | 1991-04-15 | Yeda Res & Dev | Production of interferon-ypsilon |
WO1986005514A1 (en) * | 1985-03-22 | 1986-09-25 | Chiron Corporation | EXPRESSION OF tPA IN MAMMALIAN CELLS |
NZ221790A (en) * | 1986-09-12 | 1990-07-26 | Genentech Inc | Method for the continuous production of a heterologous protein in a eukaryotic host cell |
-
1985
- 1985-12-19 DE DE19853545126 patent/DE3545126A1/de not_active Withdrawn
-
1986
- 1986-12-15 IL IL80960A patent/IL80960A0/xx unknown
- 1986-12-18 ZA ZA869487A patent/ZA869487B/xx unknown
- 1986-12-18 PT PT83968A patent/PT83968B/pt not_active IP Right Cessation
- 1986-12-19 AU AU68333/87A patent/AU600253B2/en not_active Ceased
- 1986-12-19 AT AT87900133T patent/ATE67242T1/de not_active IP Right Cessation
- 1986-12-19 JP JP62500485A patent/JPS62502942A/ja active Pending
- 1986-12-19 ES ES198787900133T patent/ES2026206T3/es not_active Expired - Lifetime
- 1986-12-19 WO PCT/EP1986/000763 patent/WO1987003904A1/de active IP Right Grant
- 1986-12-19 DE DE8787900133T patent/DE3681446D1/de not_active Expired - Lifetime
- 1986-12-19 EP EP86117750A patent/EP0227064A1/de not_active Withdrawn
- 1986-12-19 EP EP87900133A patent/EP0250513B1/de not_active Expired - Lifetime
-
1987
- 1987-08-18 FI FI873572A patent/FI873572A0/fi not_active Application Discontinuation
- 1987-08-19 KR KR870700745A patent/KR880700855A/ko not_active Application Discontinuation
- 1987-08-19 DK DK432987A patent/DK432987D0/da not_active Application Discontinuation
-
1991
- 1991-12-11 GR GR91401398T patent/GR3003301T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
WO1987003904A1 (en) | 1987-07-02 |
EP0227064A1 (de) | 1987-07-01 |
JPS62502942A (ja) | 1987-11-26 |
ATE67242T1 (de) | 1991-09-15 |
DK432987A (da) | 1987-08-19 |
KR880700855A (ko) | 1988-04-12 |
DK432987D0 (da) | 1987-08-19 |
DE3681446D1 (de) | 1991-10-17 |
EP0250513B1 (de) | 1991-09-11 |
IL80960A0 (en) | 1987-03-31 |
PT83968A (de) | 1987-01-01 |
AU600253B2 (en) | 1990-08-09 |
EP0250513A1 (de) | 1988-01-07 |
AU6833387A (en) | 1987-07-15 |
FI873572A (fi) | 1987-08-18 |
PT83968B (pt) | 1989-01-17 |
ZA869487B (en) | 1987-09-30 |
GR3003301T3 (en) | 1993-02-17 |
FI873572A0 (fi) | 1987-08-18 |
DE3545126A1 (de) | 1987-06-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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