PT83968B - Processo para melhoramento da expressao de proteina eucariotica, de preparacao de um plasmideo e plasmideo - Google Patents

Processo para melhoramento da expressao de proteina eucariotica, de preparacao de um plasmideo e plasmideo

Info

Publication number
PT83968B
PT83968B PT83968A PT8396886A PT83968B PT 83968 B PT83968 B PT 83968B PT 83968 A PT83968 A PT 83968A PT 8396886 A PT8396886 A PT 8396886A PT 83968 B PT83968 B PT 83968B
Authority
PT
Portugal
Prior art keywords
plasmide
protein
expression
cdna
eucarious
Prior art date
Application number
PT83968A
Other languages
English (en)
Other versions
PT83968A (de
Original Assignee
Boehringer Mannheim Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh
Publication of PT83968A publication Critical patent/PT83968A/pt
Publication of PT83968B publication Critical patent/PT83968B/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6456Plasminogen activators
    • C12N9/6459Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21Serine endopeptidases (3.4.21)
    • C12Y304/21069Protein C activated (3.4.21.69)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/03Automatic limiting or abutting means, e.g. for safety
    • A61B2090/033Abutting means, stops, e.g. abutting on tissue or skin
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B90/00Instruments, implements or accessories specially adapted for surgery or diagnosis and not covered by any of the groups A61B1/00 - A61B50/00, e.g. for luxation treatment or for protecting wound edges
    • A61B90/06Measuring instruments not otherwise provided for
    • A61B2090/062Measuring instruments not otherwise provided for penetration depth

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Networks Using Active Elements (AREA)
  • Saccharide Compounds (AREA)
PT83968A 1985-12-19 1986-12-18 Processo para melhoramento da expressao de proteina eucariotica, de preparacao de um plasmideo e plasmideo PT83968B (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19853545126 DE3545126A1 (de) 1985-12-19 1985-12-19 Verfahren zur verbesserung der expression von eukaryontenprotein

Publications (2)

Publication Number Publication Date
PT83968A PT83968A (de) 1987-01-01
PT83968B true PT83968B (pt) 1989-01-17

Family

ID=6288962

Family Applications (1)

Application Number Title Priority Date Filing Date
PT83968A PT83968B (pt) 1985-12-19 1986-12-18 Processo para melhoramento da expressao de proteina eucariotica, de preparacao de um plasmideo e plasmideo

Country Status (14)

Country Link
EP (2) EP0250513B1 (pt)
JP (1) JPS62502942A (pt)
KR (1) KR880700855A (pt)
AT (1) ATE67242T1 (pt)
AU (1) AU600253B2 (pt)
DE (2) DE3545126A1 (pt)
DK (1) DK432987D0 (pt)
ES (1) ES2026206T3 (pt)
FI (1) FI873572A0 (pt)
GR (1) GR3003301T3 (pt)
IL (1) IL80960A0 (pt)
PT (1) PT83968B (pt)
WO (1) WO1987003904A1 (pt)
ZA (1) ZA869487B (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988005466A2 (en) * 1987-01-15 1988-07-28 Codon Tandem gene eukaryotic expression vectors
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
DE3730246A1 (de) * 1987-09-09 1989-06-01 Boehringer Mannheim Gmbh Expressionsvektor und verfahren zur expression von heterologen proteinen in saeugerzellen
CA2018676C (en) * 1989-06-20 2003-05-06 Christie A. Kelton Novel heteropolymeric protein production methods
FR2650838A1 (fr) * 1989-08-09 1991-02-15 Transgene Sa Vecteurs d'expression du facteur ix dans une cellule eucaryote superieure, procede de preparation de facteur ix par des animaux transgeniques et facteur ix obtenu
EP0451700A1 (en) * 1990-04-10 1991-10-16 Miles Inc. Recombinant APP minigenes for expression in transgenic mice as models for Alzheimers's disease
AU1180692A (en) * 1991-01-29 1992-08-27 Teijin Limited Human protein c expression vector
US6627615B1 (en) 1991-12-17 2003-09-30 The Regents Of The University Of California Methods and compositions for in vivo gene therapy
US5756353A (en) * 1991-12-17 1998-05-26 The Regents Of The University Of California Expression of cloned genes in the lung by aerosol-and liposome-based delivery
US5858784A (en) * 1991-12-17 1999-01-12 The Regents Of The University Of California Expression of cloned genes in the lung by aerosol- and liposome-based delivery
EP0646178A1 (en) * 1992-06-04 1995-04-05 The Regents Of The University Of California expression cassette with regularoty regions functional in the mammmlian host
US6806084B1 (en) 1992-06-04 2004-10-19 The Regents Of The University Of California Methods for compositions for in vivo gene delivery
AU2660397A (en) * 1996-04-05 1997-10-29 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide bond-containing eukaryotic proteins in bacterial cells
EP1669454A3 (en) * 1996-06-27 2009-04-01 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Genomic DNA encoding a polypeptide capable of inducing the production of interferon-gamma
JP2002517180A (ja) * 1997-12-05 2002-06-18 ジ・イミユーン・リスポンス・コーポレーシヨン 増大された発現を示す新規ベクターおよび遺伝子
SE9901339D0 (sv) * 1999-04-15 1999-04-15 Astra Ab New expression systems
GB201106380D0 (en) 2011-04-15 2011-06-01 Nokia Corp An apparatus, method, computer program and user interface

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2534273B1 (fr) * 1982-10-07 1985-09-13 Pasteur Institut Lignees cellulaires originaires de mammiferes superieurs, productrices d'hormone de croissance, de preference humaine, et vecteurs pour leur obtention
FR2535243B1 (fr) * 1982-11-03 1986-04-04 Fives Cail Babcock Procede de fabrication d'un cylindre, notamment pour moulin a cannes, et cylindre obtenu par ce procede
IL71691A (en) * 1984-04-27 1991-04-15 Yeda Res & Dev Production of interferon-ypsilon
JPH0740942B2 (ja) * 1985-03-22 1995-05-10 カイロン コ−ポレイシヨン 哺乳動物細胞におけるtPAの発現
DK175363B1 (da) * 1986-09-12 2004-09-13 Genentech Inc Fremgangsmåde til kontinuert produktion af et önsket heterologt protein i en eukaryotisk værtcelle, vektor, som tilförer denne evne, og celler transformeret med vektoren

Also Published As

Publication number Publication date
WO1987003904A1 (en) 1987-07-02
IL80960A0 (en) 1987-03-31
FI873572A (fi) 1987-08-18
ZA869487B (en) 1987-09-30
GR3003301T3 (en) 1993-02-17
DK432987A (da) 1987-08-19
ES2026206T3 (es) 1992-04-16
KR880700855A (ko) 1988-04-12
FI873572A0 (fi) 1987-08-18
DE3545126A1 (de) 1987-06-25
JPS62502942A (ja) 1987-11-26
AU600253B2 (en) 1990-08-09
EP0250513B1 (de) 1991-09-11
PT83968A (de) 1987-01-01
ATE67242T1 (de) 1991-09-15
EP0250513A1 (de) 1988-01-07
DK432987D0 (da) 1987-08-19
EP0227064A1 (de) 1987-07-01
AU6833387A (en) 1987-07-15
DE3681446D1 (de) 1991-10-17

Similar Documents

Publication Publication Date Title
PT83968B (pt) Processo para melhoramento da expressao de proteina eucariotica, de preparacao de um plasmideo e plasmideo
BR8301918A (pt) Proteina,sequencia de dna,veiculo de expressao replicavel,microorganismo ou cultura celular,plasmideo,composicao,cultura de celulas,utilizacao de tal proteina,processo de expressao,processo para producao de um veiculo de expressao e processo para preparacao de uma proteina uroquinase
DE3479321D1 (en) Biosynthetically produced human nerve growth factor, process for producing it, compositions containing it, dna sequence encoding it, vectors containing the sequence and host cells transformed thereby
EP0343783A3 (en) Method for producing cells containing stably integrated foreign dna at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign dna
AU5233686A (en) Dna sequence encoding a hirudin-like protein and process for preparing such protein
FI944136A0 (fi) Koostumuksia ja menetelmä sialyylitransferaasien identifioimiseksi ja syntesoimiseksi
IL70414A (en) Polypeptide having human h2-relaxin activity,double-stranded dna fragments coding therefor,vectors containing the dna fragments and methods for preparing the polypeptide,dna fragments and vectors
DE69942861D1 (de) Ldcam genannte moleküle
KR910004803A (ko) 간의 실질 세포 성장 인자, 이를 코딩하는 유전자, 이 인자를 생산하는 방법, 및 이 인자를 생산하는 형질전환체
IL75133A (en) Process for the preparation of 1,2-diacyl-s,n-glycerophosphocholines(or glycerophosphoethanolamines)and some novel intermediates therefor
ES545725A0 (es) Procedimiento para preparar peptidos de interferon
PT94680A (pt) Processo para a obtencao de uma proteina tendo actividade de urato oxidase gene recombinante que a codifica vector de expressao portador deste gene microorganismos procariotas e celulas eucariotas transformados por este vector de expressao
ATE108831T1 (de) Rinder-interleukin-1-alpha.
GR852288B (pt)
DK315582A (da) T4 dna-fragment som stabilisator for proteiner udtrykt ved klonet dna
ATE315644T1 (de) Für das igg-fc-bindende protein kodierendes gen
ATE173762T1 (de) Klonierung der n-methylhydantoinase
HUT47976A (en) Process for producing recombinant expression dna vectors encoding isopenicillin-n-synthetase enzyme of aspergillus nidulans, as well as dna molecules
EP0422217A4 (en) Human recombinant placental ribonuclease inhibitor and method of production
ATE87977T1 (de) Herstellung menschlichen somatomedins c.
ES550868A0 (es) Un procedimiento para la produccion de proquimosina
EP0183272A3 (en) Vector capable of efficient gene expression and utilization of such vector
DE69104357D1 (de) Klonierungs- und/oder expressionsvektoren, verfahren zur ihrer herstellung und verwendung.
DE3853590D1 (de) Rinder granulocyt-makrophagenkolonie stimulierender faktor.
LTIP1543A (en) Process for enzymatic decomposition of recombinant proteins by means of iga-protease, fused protein, recombinant dna, cell

Legal Events

Date Code Title Description
MM3A Annulment or lapse

Free format text: LAPSE DUE TO NON-PAYMENT OF FEES

Effective date: 19911223