ES2045127T3 - Metodo de multiplicacion para ensayos de polinucleotidos. - Google Patents

Metodo de multiplicacion para ensayos de polinucleotidos.

Info

Publication number
ES2045127T3
ES2045127T3 ES88306717T ES88306717T ES2045127T3 ES 2045127 T3 ES2045127 T3 ES 2045127T3 ES 88306717 T ES88306717 T ES 88306717T ES 88306717 T ES88306717 T ES 88306717T ES 2045127 T3 ES2045127 T3 ES 2045127T3
Authority
ES
Spain
Prior art keywords
polynucleotide
template
fragments
extension
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88306717T
Other languages
English (en)
Inventor
Martin Becker
Thomas Goodman
Samual Rose
Edwin F Ullman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Syntex USA LLC
Original Assignee
Syntex USA LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syntex USA LLC filed Critical Syntex USA LLC
Application granted granted Critical
Publication of ES2045127T3 publication Critical patent/ES2045127T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

SE DESCRIBE UN METODO PARA PRODUCIR COPIAS MULTIPLES DE UNA SECUENCIA DE POLINUCLEOTIDO PRIMARIA, LOCALIZADA EN EL TERMINO 3'' DE UN POLINUCLEOTIDO. EL METODO CONSISTE EN (A) FORMAR, EN PRESENCIA DE TRIFOSFATOS DE NUCLEOSIDO Y POLIMERASA DE POLINUCLEOTIDO DEPENDIENTE DE PLANTILLA, UNA EXTENSION DE UNA SECUENCIA DE NUCLEOTIDO PRIMARIA HIBRIDIZADA CON UNA SECUENCIA DE PLANTILLA DE UN NUCLEOTIDO PATRON, HECHO HEBRA SENCILLO, QUE CONTIENE 2 O MAS SECUENCIAS DE PLANTILLA, QUE CONTIENE CADA UNA UNO O MAS SITIOS ROMPIBLES; (B) ROMPER EN FRAGMENTOS DICHA EXTENSION EN SITIOS ROMPIBLES, EN PRESENCIA DE MEDIOS PARA ROMPER ESPECIFICAMENTE DICHOS SITIOS ROMPIBLES, CUANDO DICHA EXTENSION ESTA HIBRIDIZADA CON DICHA SECUENCIA DE PLANTILLA; (C) SEPARAR DICHOS FRAGMENTOS; (D) HIBRIDIZAR DICHOS FRAGMENTOS CON POLINUCLEOTIDO PATRON, HECHO HEBRA SENCILLO, Y REPETIR LAS ETAPAS (A)-(D). LAS ETAPAS (A)-(D) SE PUEDEN REALIZAR DE MODO SIMULTANEO O SECUENCIAL, PARCIAL O TOTALMENTE. EL METODO SE PUEDE APLICAR PARA LADETECCION DE UN ANALITO POLINUCLEOTIDO EN UNA MUESTRA, QUE SE SOSPECHA CONTIENE TAL ANALITO PARA FACILITAR TAL DETECCION. TAMBIEN SE DESCRIBEN COMPOSICIONES PARA LLEVAR A CABO EL METODO DE LA INVENCION.
ES88306717T 1987-07-23 1988-07-21 Metodo de multiplicacion para ensayos de polinucleotidos. Expired - Lifetime ES2045127T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/076,807 US4994368A (en) 1987-07-23 1987-07-23 Amplification method for polynucleotide assays

Publications (1)

Publication Number Publication Date
ES2045127T3 true ES2045127T3 (es) 1994-01-16

Family

ID=22134303

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88306717T Expired - Lifetime ES2045127T3 (es) 1987-07-23 1988-07-21 Metodo de multiplicacion para ensayos de polinucleotidos.

Country Status (8)

Country Link
US (1) US4994368A (es)
EP (1) EP0300796B1 (es)
JP (1) JP2788034B2 (es)
AT (1) ATE96180T1 (es)
AU (1) AU622863B2 (es)
CA (1) CA1301672C (es)
DE (1) DE3885027T2 (es)
ES (1) ES2045127T3 (es)

Families Citing this family (108)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US5622820A (en) * 1988-03-10 1997-04-22 City Of Hope Method for amplification and detection of RNA and DNA sequences
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
GB8818020D0 (en) * 1988-07-28 1988-09-01 Ici Plc Method for amplification of nucleotide sequences
US5185243A (en) * 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
FR2648152A1 (fr) * 1989-06-12 1990-12-14 Oris Ind Cie Procede de detection et/ou d'identification d'acides nucleiques par amplification et hybridation en solution et ses applications
US5766849A (en) * 1989-07-11 1998-06-16 Gen-Probe Incorporated Methods of amplifying nucleic acids using promoter-containing primer sequence
US6589734B1 (en) 1989-07-11 2003-07-08 Gen-Probe Incorporated Detection of HIV
US7009041B1 (en) 1989-07-11 2006-03-07 Gen-Probe Incorporated Oligonucleotides for nucleic acid amplification and for the detection of Mycobacterium tuberculosis
CA2020958C (en) * 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5480784A (en) * 1989-07-11 1996-01-02 Gen-Probe Incorporated Nucleic acid sequence amplification methods
EP0409159B1 (en) * 1989-07-18 1998-11-25 Shimadzu Corporation Method for testing causative microorganism of food poisoning and reagents therefor
ATE137264T1 (de) * 1989-09-06 1996-05-15 Pasteur Institut Nukleinsäurefragmente von einem genom eines geeigneten mykobakteriums, ihre verwendung zur diagnose von mykobakterieninfektionen sowie diese fragmente enthaltende plasmide
WO1991010748A1 (en) * 1990-01-16 1991-07-25 Collaborative Research, Inc. Dna sequences from specific human genomic loci useful for identification of individuals
EP0461496B1 (en) 1990-06-08 1996-09-04 BEHRINGWERKE Aktiengesellschaft Method for the quantitative determination of DNA sequences
JPH05509231A (ja) * 1990-07-24 1993-12-22 ザ ユーエイビー リサーチ ファンデーション ウシｒｓウイルスの組換えｄｎａ、タンパク質ワクチン、抗体、および形質転換細胞の使用法
US6730305B1 (en) 2000-05-08 2004-05-04 The Uab Research Foundation Nucleotide sequences encoding bovine respiratory syncytial virus immunogenic proteins
EP0502180B1 (en) * 1990-09-21 1997-04-16 Amgen Inc. Enzymatic synthesis of oligonucleotides
US5645987A (en) * 1990-09-21 1997-07-08 Amgen Inc. Enzymatic synthesis of oligonucleotides
US5525462A (en) * 1991-05-02 1996-06-11 Toyo Boseki Kabushiki Kaisha Nucleic acid sequence amplification method, detection method, and reagent kit therefor
WO1992020820A1 (en) * 1991-05-15 1992-11-26 Vanderbilt University Method to determine metastatic potential of tumor cells
US5294534A (en) * 1991-08-13 1994-03-15 Miles, Inc. Amplification method for polynucleotide assays
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5994069A (en) * 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5612199A (en) * 1991-10-11 1997-03-18 Behringwerke Ag Method for producing a polynucleotide for use in single primer amplification
USH1985H1 (en) 1992-01-09 2001-08-07 The United States Of America As Represented By The Secretary Of The Navy Method for detecting biological toxins
AU681082B2 (en) * 1992-05-06 1997-08-21 Gen-Probe Incorporated Nucleic acid sequence amplification method, composition and kit
KR950701979A (ko) * 1992-06-08 1995-05-17 다니엘 엘. 캐시앙 단핵 세포로부터 핵산의 조제 방법(Preparation of Nucleic Acid from Mononuclear Cells)
CA2141430C (en) * 1992-08-04 2009-05-12 Sherrol H. Mcdonough Nucleic acid sequence amplification
US6673616B1 (en) * 1992-12-07 2004-01-06 Third Wave Technologies, Inc. Methods and compositions for characterizing nucleic acids
US6372424B1 (en) * 1995-08-30 2002-04-16 Third Wave Technologies, Inc Rapid detection and identification of pathogens
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
DE4331012A1 (de) * 1993-09-13 1995-03-16 Bayer Ag Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik
DE4331011A1 (de) * 1993-09-13 1995-03-16 Bayer Ag Nukleinsäuren-bindende Oligomere mit C-Verzweigung für Therapie und Diagnostik
US5824787A (en) * 1993-12-03 1998-10-20 Gensura Laboratories, Inc. Polynucleotide sizing reagent
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6406855B1 (en) 1994-02-17 2002-06-18 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6335160B1 (en) * 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US20060257890A1 (en) * 1996-05-20 2006-11-16 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6395547B1 (en) 1994-02-17 2002-05-28 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6995017B1 (en) 1994-02-17 2006-02-07 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6309883B1 (en) 1994-02-17 2001-10-30 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
KR100230718B1 (ko) * 1994-03-16 1999-11-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 등온 가닥 변위 핵산 증폭법
EP0705905B1 (de) * 1994-07-16 2001-10-10 Roche Diagnostics GmbH Verfahren zum sensitiven Nachweis von Nukleinsäuren
US5597697A (en) * 1994-09-30 1997-01-28 Diamond; Paul Screening assay for inhibitors and activators of RNA and DNA-dependent nucleic acid polymerases
DE4441626A1 (de) * 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US5668263A (en) * 1994-12-16 1997-09-16 Smithkline Beecham Corporation Conserved yeast nucleic acid sequences
US5877280A (en) * 1995-06-06 1999-03-02 The Mount Sinai School Of Medicine Of The City University Of New York Thermostable muts proteins
US5660989A (en) * 1995-06-07 1997-08-26 Merck & Co., Inc. DNA polymerase extension assay for influenza virus endonuclease
US5739311A (en) * 1995-06-07 1998-04-14 Gen-Probe Incorporated Enzymatic synthesis of phosphorothioate oligonucleotides using restriction endonucleases
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
EP0833945B1 (en) * 1995-06-07 2002-01-30 Merck & Co., Inc. Dna polymerase extension assay
US5932450A (en) * 1995-06-07 1999-08-03 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using digestible templates
US6506602B1 (en) 1996-03-25 2003-01-14 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US6100028A (en) * 1996-06-03 2000-08-08 Merck & Co., Inc. DNA polymerase extension assay
US20070009930A1 (en) * 1996-12-18 2007-01-11 Maxygen, Inc. Methods and compositions for polypeptide engineering
US6482590B1 (en) 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
US6153410A (en) * 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6136568A (en) * 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
IL122147A0 (en) * 1997-11-10 1998-04-05 Technion Res & Dev Foundation Method for labeling polynucleotides
JP3712255B2 (ja) 1997-12-08 2005-11-02 カリフォルニア・インスティチュート・オブ・テクノロジー ポリヌクレオチドおよびポリペプチド配列を生成するための方法
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7153655B2 (en) * 1998-06-16 2006-12-26 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence
US6365408B1 (en) 1998-06-19 2002-04-02 Maxygen, Inc. Methods of evolving a polynucleotides by mutagenesis and recombination
GB9822777D0 (en) 1998-10-20 1998-12-16 Tepnel Medical Ltd Template chain reaction
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6955879B2 (en) * 2000-11-10 2005-10-18 Amicogen, Inc. Method for generating recombinant DNA library using unidirectional single-stranded DNA fragments
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
AU2002314712C1 (en) 2001-02-02 2008-10-02 Novici Biotech Llc A method of increasing complementarity in a heteroduplex polynucleotide
US20050153283A1 (en) * 2002-08-08 2005-07-14 Padgett Hal S. Method of increasing complementarity in a heteroduplex
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7582423B2 (en) * 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20050106587A1 (en) * 2001-12-21 2005-05-19 Micronas Gmbh Method for determining of nucleic acid analytes
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
US20030157495A1 (en) * 2002-02-01 2003-08-21 Padgett Hal S. Nucleic acid molecules encoding CEL I endonuclease and methods of use thereof
US7262012B2 (en) * 2002-05-17 2007-08-28 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function using varied exonuclease digestion in two polynucleotide populations
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
EP1766090B1 (en) * 2004-05-25 2011-04-27 Helicos Biosciences Corporation Methods for nucleic acid immobilisation
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
US20090305248A1 (en) * 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
EP2242858A4 (en) * 2008-01-08 2011-01-26 Zygem Corp Ltd ISOTHERMAL DETECTION METHODS AND USES THEREOF
CN106795522A (zh) * 2014-10-17 2017-05-31 上海斯丹赛生物技术有限公司 定向克隆的方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4663283A (en) * 1980-03-24 1987-05-05 Genentech, Inc. Method of altering double-stranded DNA
US4358535A (en) * 1980-12-08 1982-11-09 Board Of Regents Of The University Of Washington Specific DNA probes in diagnostic microbiology
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US4480040A (en) * 1981-12-03 1984-10-30 The United States Of America As Represented By The Secretary Of Agriculture Sensitive and rapid diagnosis of viroid diseases and viruses
US4423153A (en) * 1981-12-03 1983-12-27 Board Of Regents, The University Of Texas System Methods and compositions for the detection and determination of cellular DNA
US4656134A (en) * 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US4490472A (en) * 1982-06-17 1984-12-25 Imreg, Inc. Sensitive tests for malignancies based on DNA detection
JPS6091999A (ja) * 1983-10-25 1985-05-23 Fujirebio Inc ポリヌクレオチドの測定方法
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4734363A (en) * 1984-11-27 1988-03-29 Molecular Diagnostics, Inc. Large scale production of DNA probes
US4808525A (en) * 1985-01-31 1989-02-28 University Of Georgia Research Center Site specific cleavage of DNA
CA1278755C (en) * 1985-03-15 1991-01-08 William J. Knowles Labelling of oligonucleotides
DK171161B1 (da) * 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
AU606043B2 (en) * 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4746604A (en) * 1985-05-24 1988-05-24 Enzo Biochem, Inc. Specific binding assays utilizing a viable cell as a label
US4868104A (en) 1985-09-06 1989-09-19 Syntex (U.S.A.) Inc. Homogeneous assay for specific polynucleotides
US4876187A (en) * 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
BE904402A (fr) * 1986-03-12 1986-09-12 Univ Libre De Bruxelles Iribhn Moule de sonde de detection de sequende d'adn et preparation d'un tel moule et d'une telle sonde.

Also Published As

Publication number Publication date
AU622863B2 (en) 1992-04-30
US4994368A (en) 1991-02-19
AU1928588A (en) 1989-01-27
DE3885027D1 (de) 1993-11-25
EP0300796A3 (en) 1989-07-19
EP0300796B1 (en) 1993-10-20
DE3885027T2 (de) 1994-02-10
EP0300796A2 (en) 1989-01-25
CA1301672C (en) 1992-05-26
JPS6460390A (en) 1989-03-07
ATE96180T1 (de) 1993-11-15
JP2788034B2 (ja) 1998-08-20

Similar Documents

Publication Publication Date Title
ES2045127T3 (es) Metodo de multiplicacion para ensayos de polinucleotidos.
ES2093633T3 (es) Amplificacion de acidos nucleicos utilizando un unico cebador.
PL278176A1 (en) Method of detecting sequences of nucleotides and sets for carrying out diagnostic tests
ES2058112T3 (es) Sondas de hibridacion.
PT96776A (pt) Metodo e equipamento para a determinacao de variacoes especificas de nucleotidos e processo para a preparacao dos reagentes ai utilizados
KR920702726A (ko) 증폭과정중의 증폭산물 오염을 감소시키는 방법 및 폴리머라제 사슬 반응유형과 리가제 사슬반응 유형의 증폭과정에 사용하기 위한 키트, 표적 시퀀스의 증폭 시퀀스를 증폭시키는 방법
ATE348191T1 (de) Polynukleotid-sequenzassay
ATE196322T1 (de) Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
KR920014937A (ko) 사슬 치환에 의한 증폭방법
KR900016473A (ko) 진단 키트, 프라이머 조성물 및 이것을 이용해 핵산을 복제하거나 검사하는 방법
KR910006497A (ko) 프라이머 3' 말단에서의 프라이머-표적 오합(mismatch)을 극복한 진단 및 증폭방법
DK0666927T3 (da) Fremgangsmåde til påvisning af en nukleinsyresekvens.
ATE78301T1 (de) Molekulare genetische sonde und verfahren zu ihrer herstellung, testmethode und satz in denen diese molekulare genetische sonde gebraucht wird.
WO1998024928A3 (en) Detection of chromosomal abnormalities

Legal Events

Date Code Title Description
FG2A Definitive protection

Ref document number: 300796

Country of ref document: ES