ES2025553T3 - Un metodo de espectroscopia para la deteccion de una secuencia diana polinucleotidica de hebra simple. - Google Patents

Un metodo de espectroscopia para la deteccion de una secuencia diana polinucleotidica de hebra simple.

Info

Publication number
ES2025553T3
ES2025553T3 ES198686116652T ES86116652T ES2025553T3 ES 2025553 T3 ES2025553 T3 ES 2025553T3 ES 198686116652 T ES198686116652 T ES 198686116652T ES 86116652 T ES86116652 T ES 86116652T ES 2025553 T3 ES2025553 T3 ES 2025553T3
Authority
ES
Spain
Prior art keywords
fluorophores
probes
hebra
spectroscopy
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES198686116652T
Other languages
English (en)
Inventor
Michael J. Heller
Edward J. Jablonski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Molecular Biosystems Inc
Original Assignee
Molecular Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Biosystems Inc filed Critical Molecular Biosystems Inc
Application granted granted Critical
Publication of ES2025553T3 publication Critical patent/ES2025553T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/705—Specific hybridization probes for herpetoviridae, e.g. herpes simplex, varicella zoster

Abstract

LAS SONDAS PARA EL DESPLAZAMIENTO DE LOS IMPACTOS FLUORESCENTES PARA ENSAYOS DE HIBRIDACION EN LOS QUE INTERVIENEN POLINUCLEOTIDOS ESTAN DISEÑADAS DE TAL MODO QUE PROPORCIONAN UNAS SEPARACIONES UNITARIAS CON BASE NUCLEOTIDA ENTRE EL DONANTE Y EL ACEPTOR DE FLUOROFOROS. CUANDO LAS SONDAS SE HIBRIDIZAN AL POLINUCLEOTIDO OBJETIVO, LOS FLUOROFOROS EMPAREJADOS PARA UNA TRANSFERENCIA DE ENERGIA NO RADIATIVA SE SEPARAN EN DE 2 A 7 UNIDADES CON BASE NUCLEOTIDA. LOS FLUOROFOROS QUEDAN UNIDOS A LAS SONDAS DE ADN O DE ARN MEDIANTE UNOS BRAZOS ENLAZADORES QUE TIENEN UNAS LONGITUDES DE 4 A 30 ANGSTROMS. UN DONANTE PREFERENTE ES LA FLUORESCEINA PARA UTILIZARLA CON UN ACEPTOR DE ROJO DE TEJAS.
ES198686116652T 1985-12-23 1986-12-01 Un metodo de espectroscopia para la deteccion de una secuencia diana polinucleotidica de hebra simple. Expired - Lifetime ES2025553T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US81211185A 1985-12-23 1985-12-23

Publications (1)

Publication Number Publication Date
ES2025553T3 true ES2025553T3 (es) 1992-04-01

Family

ID=25208540

Family Applications (1)

Application Number Title Priority Date Filing Date
ES198686116652T Expired - Lifetime ES2025553T3 (es) 1985-12-23 1986-12-01 Un metodo de espectroscopia para la deteccion de una secuencia diana polinucleotidica de hebra simple.

Country Status (7)

Country Link
EP (1) EP0229943B1 (es)
JP (1) JPH0763400B2 (es)
AT (1) ATE66962T1 (es)
CA (1) CA1273552A (es)
DE (1) DE3681272D1 (es)
ES (1) ES2025553T3 (es)
GR (1) GR3002735T3 (es)

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Also Published As

Publication number Publication date
CA1273552A (en) 1990-09-04
EP0229943A2 (en) 1987-07-29
EP0229943B1 (en) 1991-09-04
DE3681272D1 (de) 1991-10-10
JPH0763400B2 (ja) 1995-07-12
JPS62157570A (ja) 1987-07-13
EP0229943A3 (en) 1987-11-11
GR3002735T3 (en) 1993-01-25
ATE66962T1 (de) 1991-09-15

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