ES2009788B3 - Metodo de mejorar la demarcacion y separacion de celulas en muestras sanguineas centrifugadas. - Google Patents

Metodo de mejorar la demarcacion y separacion de celulas en muestras sanguineas centrifugadas.

Info

Publication number
ES2009788B3
ES2009788B3 ES86308170T ES86308170T ES2009788B3 ES 2009788 B3 ES2009788 B3 ES 2009788B3 ES 86308170 T ES86308170 T ES 86308170T ES 86308170 T ES86308170 T ES 86308170T ES 2009788 B3 ES2009788 B3 ES 2009788B3
Authority
ES
Spain
Prior art keywords
cells
centrifugated
demarcation
separation
improving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES86308170T
Other languages
English (en)
Inventor
Robert A Levine
Rodolfo R Rodriguez
Michael R Loken
Steven C Wardlaw
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Application granted granted Critical
Publication of ES2009788B3 publication Critical patent/ES2009788B3/es
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5002—Partitioning blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • External Artificial Organs (AREA)
  • Centrifugal Separators (AREA)

Abstract

A FIN DE PRODUCIR UNA FASE MAS BIEN DEFINIDA ENTRE CAPAS DE CELULAS ADYACENTES EN UNA MUESTRA CENTRIFUGADA DE SANGRE INTACTA COAGULADA, POR EJEMPLO ENTRE LOS ERITROCITOS Y LOS LEUCOCITOS, SE AÑADE A LA MUESTRA DE SANGRE, ANTES DE LA CENTRIFUGACION, UN MATERIAL QUE ENLAZA SELECTIVAMENTE UN GRUPO DE CELULAS JUNTAS, POR EJEMPLO LOS ERITROCITOS. EL MATERIAL ENLAZANTE TIENE QUE PRODUCIR UNA UNION MUY RESISTENTE ENTRE UN GRUPO DE CELULAS, PERO NO AFECTAR A LOS OTROS TIPOS DE CELULAS. EL MATERIAL SE AÑADE ANTES DE CENTRIFUGAR LA MUESTRA DE SANGRE.
ES86308170T 1985-11-01 1986-10-21 Metodo de mejorar la demarcacion y separacion de celulas en muestras sanguineas centrifugadas. Expired ES2009788B3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/794,127 US4695553A (en) 1985-11-01 1985-11-01 Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample using antibodies

Publications (1)

Publication Number Publication Date
ES2009788B3 true ES2009788B3 (es) 1989-10-16

Family

ID=25161793

Family Applications (1)

Application Number Title Priority Date Filing Date
ES86308170T Expired ES2009788B3 (es) 1985-11-01 1986-10-21 Metodo de mejorar la demarcacion y separacion de celulas en muestras sanguineas centrifugadas.

Country Status (7)

Country Link
US (1) US4695553A (es)
EP (1) EP0225703B1 (es)
JP (1) JPH0823557B2 (es)
AT (1) ATE44824T1 (es)
BR (1) BR8603447A (es)
DE (1) DE3664537D1 (es)
ES (1) ES2009788B3 (es)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK17885D0 (da) * 1985-01-14 1985-01-14 Karlsson Karl Anders Antiviralt middel
US4752563A (en) * 1985-11-19 1988-06-21 Coulter Corporation Monoclonal antibody for recovery of leukocytes in human peripheral blood and method of recovery employing said monoclonal antibody
US5086002A (en) * 1987-09-07 1992-02-04 Agen Biomedical, Ltd. Erythrocyte agglutination assay
CA2011099A1 (en) * 1989-04-19 1990-10-19 Stephen C. Wardlaw Determination of lymphocyte reactivity to specific antigens in blood
US5583003A (en) * 1989-09-25 1996-12-10 Agen Limited Agglutination assay
US5641622A (en) * 1990-09-13 1997-06-24 Baxter International Inc. Continuous centrifugation process for the separation of biological components from heterogeneous cell populations
JPH07104350B2 (ja) * 1991-03-12 1995-11-13 株式会社いかがく 腫瘍、癌、前癌症状の検出用キット
DE19631855C2 (de) * 1996-08-07 1998-10-15 Komanns Aribert Verfahren und Vorrichtung zur Erfassung von Oberflächenantigenen oder Strukturmerkmalen von Zellen, Partikeln oder Makromolekülen
US5811303A (en) * 1997-04-01 1998-09-22 Streck Laboratories, Inc. Quantitative buffy coat control composition
JP2997771B2 (ja) * 1998-06-29 2000-01-11 金沢大学長 細胞の懸濁培養方法
US6153113A (en) * 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
JP6357473B2 (ja) * 2012-08-08 2018-07-11 コーニンクレッカ フィリップス エヌ ヴェＫｏｎｉｎｋｌｉｊｋｅ Ｐｈｉｌｉｐｓ Ｎ．Ｖ． 一滴の血液を使用した血漿分離
JP7173867B2 (ja) 2016-04-22 2022-11-16 ベクトン・ディキンソン・アンド・カンパニー 多重高分子色素デバイス及びその使用方法
JP6779483B2 (ja) 2016-09-29 2020-11-04 住友ゴム工業株式会社 医療用検査装置及び細胞検査方法
CN110337331B (zh) 2017-02-08 2022-04-26 贝克顿·迪金森公司 干染料试剂装置以及制造和使用所述干染料试剂装置的方法
JP7109719B2 (ja) * 2018-02-14 2022-08-01 住友ゴム工業株式会社 特定細胞捕捉方法
JP7158671B2 (ja) 2018-02-14 2022-10-24 住友ゴム工業株式会社 特定細胞捕捉方法
US11992844B2 (en) 2018-11-13 2024-05-28 Becton, Dickinson And Company Dried reagent strainers and methods for making and using the same
US11614440B2 (en) 2019-01-24 2023-03-28 Sumitomo Rubber Industries, Ltd. Specific cell fractionating and capturing methods
CN111458301B (zh) * 2019-12-24 2023-12-19 江汉大学 菜豆凝集素检测试剂盒

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3146163A (en) * 1962-01-23 1964-08-25 John H Brewer Apparatus for separating certain components from blood
US3902964A (en) * 1971-12-13 1975-09-02 U S Medical Research And Dev I Method of and apparatus for chemically separating plasma or serum from formed elements of blood
US4027660A (en) * 1976-04-02 1977-06-07 Wardlaw Stephen C Material layer volume determination
US4181609A (en) * 1977-12-20 1980-01-01 Levine Robert A Blood constituents testing method
GB2018151B (en) * 1978-03-06 1982-12-08 Asahi Chemical Ind Seperation of leukocytes from leukocyte-containing suspension by filtration
US4255256A (en) * 1978-12-13 1981-03-10 Antonio Ferrante Medium for the separation of human blood leucocytes
DE3009126A1 (de) * 1980-03-10 1981-10-08 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen Verfahren zur gewinnung von funktionell und morphologisch intakten und lebensfaehigen leukozyten aus blut
JPS56133648A (en) * 1980-03-24 1981-10-19 Joko:Kk Method and device for measuring agglutination reaction by optical method
DE3235924T1 (de) * 1981-03-06 1983-05-05 Celltech Ltd., Slough, Berkshire Monoklonaler antikoerper
JPS58102155A (ja) * 1981-12-14 1983-06-17 Olympus Optical Co Ltd 血球浮遊液作成方法
SE434680B (sv) * 1983-01-21 1984-08-06 Karl Arne Lundblad Anvendning av en saltlosning av monoklonala antikroppar med relativt hog salthalt vid blodgruppsbestemning
US4599307A (en) * 1983-07-18 1986-07-08 Becton, Dickinson And Company Method for elimination of selected cell populations in analytic cytology
US4594327A (en) * 1983-11-02 1986-06-10 Syntex (U.S.A.) Inc. Assay method for whole blood samples

Also Published As

Publication number Publication date
JPH0823557B2 (ja) 1996-03-06
EP0225703A3 (en) 1987-07-22
DE3664537D1 (en) 1989-08-24
ATE44824T1 (de) 1989-08-15
EP0225703A2 (en) 1987-06-16
US4695553A (en) 1987-09-22
EP0225703B1 (en) 1989-07-19
JPS62108158A (ja) 1987-05-19
BR8603447A (pt) 1987-11-17

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