ES2001973A6 - Procedimiento para la obtencion de una linea celular hibrida productora del anticuerpo monoclonal kc-16 y aplicaciones del mismo. - Google Patents

Procedimiento para la obtencion de una linea celular hibrida productora del anticuerpo monoclonal kc-16 y aplicaciones del mismo.

Info

Publication number
ES2001973A6
ES2001973A6 ES8601990A ES8601990A ES2001973A6 ES 2001973 A6 ES2001973 A6 ES 2001973A6 ES 8601990 A ES8601990 A ES 8601990A ES 8601990 A ES8601990 A ES 8601990A ES 2001973 A6 ES2001973 A6 ES 2001973A6
Authority
ES
Spain
Prior art keywords
monoclonal antibody
recovery
white blood
peripheral blood
blood cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8601990A
Other languages
English (en)
Inventor
Kenneth H Kortright
David E Hofheinz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coulter Corp
Original Assignee
Coulter Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Coulter Corp filed Critical Coulter Corp
Publication of ES2001973A6 publication Critical patent/ES2001973A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
    • C07F9/3808—Acyclic saturated acids which can have further substituents on alkyl
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/18—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-carbon bonds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/40—Esters thereof
    • C07F9/4003—Esters thereof the acid moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/4006—Esters of acyclic acids which can have further substituents on alkyl
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Environmental Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • External Artificial Organs (AREA)

Abstract

PROCEDIMIENTO PARA LA OBTENCION DE UNA LINEA CELULAR HIBRIDA PRODUCTORA DEL ANTICUERPO MONOCLONAL KC-16 Y APLICACIONES DEL MISMO. EL PROCEDIMIENTO COMPRENDE LA FUSION DE CELULAS PRODUCTORAS DEL ANTICUERPO CON CELULAS INMORTALES, CULTIVAR Y SELECCIONAR LOS HIBRIDOMAS PRODUCTORES DEL CITADO ANTICUERPO MONOCLONAL. TAMBIEN SE DESCRIBE UN PROCEDIMIENTO PARA LA OBTENCION DEL ANTICUERPO POR CULTIVO DEL HIBRIDOMA Y AISLAMIENTO DEL ANTICUERPO. EL ANTICUERPO MONOCLONAL KC-16 SE UNE SELECTIVAMENTE A UN EPITOPO ESPECIFICO DE LA GLICOFORINA A EXPUESTA EN LA MEMBRANA DEL ERITROCITO Y NO SE UNE A OTRAS GLICOFORINAS. EL ANTICUERPO SE APLICA SOBRE UNA MICROESFERA O SUSTRATO ADECUADO Y SE UTILIZA PARA RECUPERAR SUBPOBLACIONES DE LEUCOCITOS SIN LISAR ERITROCITOS. DE APLICACION EN EL ENSAYO PRECISO DE SUBPOBLACIONES DE LEUCOCITOS EN SANGRE PERIFERICA SIN LISIS DE ERITROCITOS.
ES8601990A 1985-11-19 1986-09-18 Procedimiento para la obtencion de una linea celular hibrida productora del anticuerpo monoclonal kc-16 y aplicaciones del mismo. Expired ES2001973A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/799,489 US4752563A (en) 1985-11-19 1985-11-19 Monoclonal antibody for recovery of leukocytes in human peripheral blood and method of recovery employing said monoclonal antibody

Publications (1)

Publication Number Publication Date
ES2001973A6 true ES2001973A6 (es) 1988-07-01

Family

ID=25176035

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8601990A Expired ES2001973A6 (es) 1985-11-19 1986-09-18 Procedimiento para la obtencion de una linea celular hibrida productora del anticuerpo monoclonal kc-16 y aplicaciones del mismo.

Country Status (17)

Country Link
US (1) US4752563A (es)
EP (1) EP0245291B1 (es)
JP (2) JPS63501332A (es)
KR (1) KR880700938A (es)
CN (1) CN1013586B (es)
AT (1) ATE75317T1 (es)
AU (1) AU590615B2 (es)
BR (1) BR8606994A (es)
CA (1) CA1294233C (es)
DE (1) DE3685023D1 (es)
DK (1) DK374987D0 (es)
ES (1) ES2001973A6 (es)
IE (1) IE59433B1 (es)
IL (1) IL80082A (es)
NO (1) NO169243C (es)
WO (1) WO1987003096A1 (es)
ZA (1) ZA867150B (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5076950A (en) * 1985-12-20 1991-12-31 Syntex (U.S.A.) Inc. Magnetic composition for particle separation
NO162946C (no) * 1987-08-21 1990-03-14 Otto Soerensen Anordning for magnetisk separasjon av celler.
US5238812A (en) * 1987-03-13 1993-08-24 Coulter Corporation Method and apparatus for rapid mixing of small volumes for enhancing biological reactions
US5641622A (en) 1990-09-13 1997-06-24 Baxter International Inc. Continuous centrifugation process for the separation of biological components from heterogeneous cell populations
CA2096530A1 (en) * 1990-11-23 1992-05-24 Thomas Russell Method and apparatus for screening microscopic cells utilizing light scatter techniques
EP0574508B1 (en) * 1991-02-22 1997-11-12 Coulter Corporation Method and apparatus for screening microscopic cells utilizing polarized light scatter techniques
US5227369A (en) * 1991-07-11 1993-07-13 The Regents Of The University Of California Compositions and methods for inhibiting leukocyte adhesion to cns myelin
US5449619A (en) * 1992-04-16 1995-09-12 Sybron Chemical Holdings, Inc. Drain opener formulation
US5763204A (en) * 1992-09-14 1998-06-09 Coulter Corporation Preparation of preserved, non-infectious control cell for use in the identification of a disease through blood testing
US6140040A (en) * 1995-10-06 2000-10-31 Advanced Minerals Corporation Method of mechanically separating microparticles suspended in fluids using particulate media
US5594116A (en) * 1995-11-08 1997-01-14 Promega Corporation Tryptase polyclonal antibody and purification method for use in human tryptase immunoassay
DE69626418T2 (de) * 1995-11-13 2003-11-13 Biotransplant, Inc. Verfahren zur bulk-anreicherung von einer zellpopulation oder zellsubpopulation
BR9706998A (pt) * 1996-01-16 1999-07-20 Sybron Chemical Holding Inc Fomulação líquida limpadora e higienizadora
US5814468A (en) * 1996-03-27 1998-09-29 Coulter International Corp. Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution
US6153113A (en) * 1999-02-22 2000-11-28 Cobe Laboratories, Inc. Method for using ligands in particle separation
US20020058030A1 (en) * 2000-05-03 2002-05-16 Eligix, Inc. Whole blood separator apparatus and method of use
US8263404B2 (en) * 2005-04-08 2012-09-11 Medical Discovery Partners Llc Method for enriching rare cell subpopulations from blood
US8409818B2 (en) * 2006-09-28 2013-04-02 The Macfarlane Burnet Institute For Medical Research And Public Health Limited Method of diagnosis and kit therefor
US20080171399A1 (en) * 2006-10-09 2008-07-17 Independent Forensics, Inc. Forensic Test for Human Blood
FR2963108B1 (fr) * 2010-07-21 2017-06-23 Diagast Procede magnetique d'immunodiagnostic et kit pour la mise en evidence de complexe anticorps/antigene de groupe/phenotype sanguin
CN118388650B (zh) * 2024-06-28 2024-08-27 杭州赛基生物科技有限公司 一种抗CD235a单克隆抗体及其制备方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3700555A (en) * 1970-10-12 1972-10-24 Technicon Instr Method and apparatus for lymphocyte separation from blood
US4230685A (en) * 1979-02-28 1980-10-28 Northwestern University Method of magnetic separation of cells and the like, and microspheres for use therein
US4598051A (en) * 1980-03-12 1986-07-01 The Regents Of The University Of California Liposome conjugates and diagnostic methods therewith
US4375407A (en) * 1981-06-22 1983-03-01 The Franklin Institute High gradient magnetic separation device
US4429008B1 (en) * 1981-12-10 1995-05-16 Univ California Thiol reactive liposomes
NO155316C (no) * 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
GB8318575D0 (en) * 1983-07-08 1983-08-10 Cobbold S P Antibody preparations
US4594327A (en) * 1983-11-02 1986-06-10 Syntex (U.S.A.) Inc. Assay method for whole blood samples
US4695553A (en) * 1985-11-01 1987-09-22 Becton Dickinson And Co., Inc. Method for increasing agglutination of groups of cells to produce improved cell layer interface in centrifuged blood sample using antibodies
US6450694B1 (en) * 2000-06-20 2002-09-17 Corona Optical Systems, Inc. Dynamically configurable backplane

Also Published As

Publication number Publication date
US4752563A (en) 1988-06-21
NO169243C (no) 1992-05-27
DK374987A (da) 1987-07-17
BR8606994A (pt) 1987-12-01
IE59433B1 (en) 1994-02-23
NO169243B (no) 1992-02-17
JPH09327287A (ja) 1997-12-22
WO1987003096A1 (en) 1987-05-21
IL80082A0 (en) 1986-12-31
EP0245291A4 (en) 1988-06-23
NO872934L (no) 1987-07-14
EP0245291B1 (en) 1992-04-22
IE862504L (en) 1987-05-19
ATE75317T1 (de) 1992-05-15
CA1294233C (en) 1992-01-14
KR880700938A (ko) 1988-04-13
NO872934D0 (no) 1987-07-14
AU590615B2 (en) 1989-11-09
IL80082A (en) 1990-12-23
CN86107203A (zh) 1987-09-30
DK374987D0 (da) 1987-07-17
ZA867150B (en) 1988-05-25
JPS63501332A (ja) 1988-05-26
CN1013586B (zh) 1991-08-21
EP0245291A1 (en) 1987-11-19
DE3685023D1 (de) 1992-05-27
AU6283686A (en) 1987-06-02

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