JP6779483B2 - 医療用検査装置及び細胞検査方法 - Google Patents
医療用検査装置及び細胞検査方法 Download PDFInfo
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- JP6779483B2 JP6779483B2 JP2016191616A JP2016191616A JP6779483B2 JP 6779483 B2 JP6779483 B2 JP 6779483B2 JP 2016191616 A JP2016191616 A JP 2016191616A JP 2016191616 A JP2016191616 A JP 2016191616A JP 6779483 B2 JP6779483 B2 JP 6779483B2
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- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
Description
前記複数のウェルは、マトリクス状に配置されていることが好ましい。
前記複数のウェルは、配置が判別可能なように識別がなされていることが好ましい。
前記親水性ポリマー層は、下記式(I)で表されるポリマー及びポリ(メタ)アクリロイルモルホリンからなる群より選択される少なくとも1種の親水性ポリマーで形成されていることが好ましい。
図1の医療用検査装置1(マルチウェルプレート1)は、がん細胞を捕捉する目的で使用される装置で、いわゆるマトリクス状にウェル11(ウェル11a、11b、11c等)が配置されたマルチウェルプレート1である。マルチウェルプレート1は、円形に開口された複数のウェル11を有している。ウェル11は、血液、体液等を注入する凹部であり、注入した血液や体液のがん細胞の有無の確認、がん細胞数の計測、がん細胞の培養、薬の効き目の確認・選定を実施できる。
(1)、(2)の保持時間は、ウェル11の大きさ、導入する液種、等により適宜設定すれば良いが、5分〜10時間が好ましく、10分〜5時間がより好ましく、15分〜2時間が更に好ましい。保持後、適宜、余分な親水性ポリマー溶液・分散液を排出し、乾燥してもよい。
AIBN(アゾビスイソブチロニトリル)を用いて、2−メトキシエチルアクリレートを80℃で6時間熱重合し、ポリ2−メトキシエチルアクリレートを作製した(分子量Mn:約15000、Mw:約50000)。そして、得られたポリ2−メトキシエチルアクリレートの2.5W/V%メタノール溶液を作製した。
3個のウェルを有するポリスチレン製検査装置に、作製したポリ2−メトキシエチルアクリレート溶液(2.5W/V%)を注入し、30分室温放置した後、液をピペットで吸出し、乾燥することで、図3の親水性ポリマー層が形成されたマルチウェルプレートを有する検査装置を作製した(ポリ2−メトキシエチルアクリレート層:88nm)。
線維肉腫(HT−1080)を剥離液を用いて懸濁させ、一部をPBS溶液(リン酸緩衝生理食塩水)に混濁させて、細胞数を血球計測管でカウントした後、この値を用いて、血液に線維肉腫(HT−1080)の入った剥離液を混濁させた。このとき、播種密度(濃度)が2850cells/cm2に計算上なるように血液を混濁させた。
この血液を用いて、検査装置の各ウェルに1mlずつ注入し、37℃で1時間接着させた。その後、PBS溶液で未接着の細胞を洗浄した。ウェルを切り離し、2個のウェルを用いて、免疫染色を行い、蛍光顕微鏡でがん細胞数をカウントした。ウェル1では1560cells/cm2のHT1080細胞がカウントされた。ウェル2では1380cells/cm2のHT1080細胞がカウントされた。
このため、切り離したウェル3に培地を入れて、37℃で2日培養した。細胞のかなりの増殖が見られ、コロニーの形成が確認できた。
11、11a、11b、11c ウェル
11ax、11bx、11cx がん細胞
21 親水性ポリマー層
Claims (7)
- 複数のウェルが分離可能である請求項1記載の医療用検査装置。
- 複数のウェルがマトリクス状に配置されている請求項1又は2記載の医療用検査装置。
- 複数のウェルは、配置が判別可能なように識別がなれている請求項1〜3のいずれかに記載の医療用検査装置。
- 前記親水性ポリマー層は、膜厚が2〜200nmである請求項1〜4のいずれかに記載の医療用検査装置。
- 請求項1〜5のいずれかに記載の医療用検査装置の複数のウェルに同一の検査用血液又は体液を導入してがん細胞を捕捉させた後、該複数のウェルの少なくとも1個を該検査用血液又は体液中のがん細胞数の計測に供する細胞検査方法。
- がん細胞の計測に供していないウェルでがん細胞の培養を行い、培養により細胞数を増加させたがん細胞で薬剤の効き目を確認する請求項6記載の細胞検査方法。
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JP2016191616A JP6779483B2 (ja) | 2016-09-29 | 2016-09-29 | 医療用検査装置及び細胞検査方法 |
US15/692,960 US10641760B2 (en) | 2016-09-29 | 2017-08-31 | Medical analysis device and cell analysis method |
EP17189338.1A EP3301442B1 (en) | 2016-09-29 | 2017-09-05 | Medical analysis device and cell analysis method |
EP20197833.5A EP3812766B1 (en) | 2016-09-29 | 2017-09-05 | Medical analysis device and cell analysis method |
CN201710826376.1A CN107881080A (zh) | 2016-09-29 | 2017-09-14 | 医疗分析装置及细胞分析方法 |
US16/829,714 US11360078B2 (en) | 2016-09-29 | 2020-03-25 | Medical analysis device and cell analysis method |
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JP2020115787A (ja) * | 2019-01-24 | 2020-08-06 | 住友ゴム工業株式会社 | 細胞培養装置及び細胞培養方法 |
JP6886668B2 (ja) * | 2019-04-23 | 2021-06-16 | 住友ゴム工業株式会社 | 医療用検査装置及び細胞検査方法 |
CN112743848B (zh) * | 2020-12-14 | 2022-02-11 | 北京大学 | 一种3d打印模型表面处理方法 |
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