JP2015224332A - 刺激応答型材料とそれを用いた細胞培養容器 - Google Patents
刺激応答型材料とそれを用いた細胞培養容器 Download PDFInfo
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Abstract
Description
刺激応答性材料の原料としては、特にストレプトマイセス属の細菌が生産するポリアミノ酸を使用することが望ましい。例えば、ポリアミノ酸の1種であるポリリジンはアミノ酸からなる生体由来材料で,安価で、化学修飾も容易、アミノ基 (-NH2) を有するため担体表面上への導入が可能、という特徴があり,温度応答部位の基本骨格として適している。構造1の部分は本発明で用いられる下限臨界溶液温度を有するポリマーとしては、N−n−プロピルアクリルアミド、N−イソプロピルアクリルアミド、N−エチルアクリルアミド、N、N−ジメチルアクリルアミド、N−アクリロイルピロリジン、N−アクリロイルピペリジン、N−アクリロイルモルホリン、N−n−プロピルメタクリルアミド、N−イソプロピルメタクリルアミド、N−エチルメタクリルアミド、N、N−ジメチルメタクリルアミド、N−メタクリロイルピロリジン、N−メタクリロイルピペリジン、N−メタクリロイルモルホリン等のN置換(メタ)アクリルアミド誘導体からなるポリマー;ヒドロキシプロピルセルロース、ポリビニルアルコール部分酢化物、ポリビニルメチルエーテル、(ポリオキシエチレン−ポリオキシプロピレン)ブロックコポリマー、ポリオキシエチレンラウリルアミン等のポリオキシエチレンアルキルアミン誘導体;ポリオキシエチレンソルビタンラウレート等のポリオキシエチレンソルビタンエステル誘導体;(ポリオキシエチレンノニルフェニルエーテル)アクリレート、(ポリオキシエチレンオクチルフェニルエーテル)メタクリレート等の(ポリオキシエチレンアルキルフェニルエーテル)(メタ)アクリレート類;及び(ポリオキシエチレンラウリルエーテル)アクリレート、(ポリオキシエチレンオレイルエーテル)メタクリレート等の(ポリオキシエチレンアルキルエーテル)(メタ)アクリレート類等のポリオキシエチレン(メタ)アクリル酸エステル誘導体等が挙げられる。更に、これらのポリマー及びこれらの少なくとも2種のモノマーからなるコポリマーも利用できる。また、N−イソプロピルアクリルアミドとN−t−ブチルアクリルアミドのコポリマーも利用できる。(メタ)アクリルアミド誘導体を含むポリマーを使用する場合、このポリマーにその他の共重合可能なモノマーを、下限臨界溶液温度を有する範囲で共重合してもよい。本発明では、なかでも、N−n−プロピルアクリルアミド、N−イソプロピルアクリルアミド、N−エチルアクリルアミド、N、N−ジメチルアクリルアミド、N−アクリロイルピロリジン、N−アクリロイルピペリジン、N−アクリロイルモルホリン、N−n−プロピルメタクリルアミド、N−イソプロピルメタクリルアミド、N−エチルメタクリルアミド、N、N−ジメチルメタクリルアミド、N−メタクリロイルピロリジン、N−メタクリロイルピペリジン、N−メタクリロイルモルホリンからなる群から選ばれる少なくとも1種のモノマーからなるポリマー又はN−イソプロピルアクリルアミドとN−t−ブチルアクリルアミドのコポリマーが好ましく利用できる。
(1)ポリアミノ酸による刺激応答材料の作成
・温度応答性ポリアミノ酸の調製
温度応答性ポリアミノ酸として、ポリリシンを基にしたもの、ポリグルタミン酸を基にしたもの、ポリアスパラギン酸を基にしたものが知られており、その合成反応をそれぞれ図3、図4、図5に示す。
(i)温度応答性ポリリシンの場合
WSC290mg、NHS170mg、吉草酸130μlをガラス容器に入れた蒸留水もしくはPBS(pH5.8)4mlに溶かした。上記溶液を4℃に冷却してポリリジン140mgを加え、一晩撹拌し、反応させた。反応では37℃で2時間でも構わない。反応液を透析膜(cut-off分子量2000,Spectra/Pore製)を用いて、室温で純水中、24時間撹拌して未反応物を除去し、単離・精製した。
(ii)温度応答性ポリアスパラギン酸の場合
セパラブルフラスコ(スターラー、温度計)へNN,-dimethylfomamide(DMF) 34gを入れ、Poly(succinimide)(PSI) 9.7g(0.1mol)を溶かした。dodecylamine(LA) 6.5g(0.035mol)と2-methoxyethylamine(MOE) 4.9g(0.065mol)を上記溶液へ添加し、70℃下で6時間反応させた。反応液を大量のacetonitrileへ入れ、フィルター処理により沈殿物を回収し、回収した沈殿物(参考:回収率92%,19.4g)は60℃下で24時間乾燥させた。
(2)刺激応答材料の刺激応答性の評価
得られたポリリジン誘導体のLCSTは,1wt%溶液 (PBS7.4) の光透過率を温度可変紫外可視吸収分光装置により測定し,光透過率が90%となる温度として決定した。結果を図3に示す。28 ℃付近でLCSTを示すため、37℃で細胞を培養(疎水性)し、27℃以下で細胞を剥離(親水性)することが可能である。
(3)刺激応答材料のプレートへの固定
表面にカルボキシル基を有する6ウェルポリスチレンマイクロプレートにEDC・HClのPBS5.8溶液 (1.0 wt%, 10 mL) を37 °Cで2時間作用させた。得られた表面活性化マイクロプレートをPBS5.8にて洗浄し、ポリマーのPBS5.8溶液 (0.001-1wt%, 10 mL) を37 °Cで2時間作用させ,PBS7.4にて洗浄することでポリマー担持表面とした (図5)。
(4)細胞培養への適用
上記刺激応答材料をヒト口腔細胞又はヒト角膜細胞の細胞シート化培養に適用した事例を示す。ヒト口腔細胞又はヒト角膜細胞は付着細胞であり、培養容器面上で最初、未分化の状態で増殖する。細胞がコンフレントな状態に達すると細胞が層状に増殖する。このとき、細胞は分化誘導をはじめ、十分に分化誘導が終了した時点で細胞を回収する(図9参照)。通常は、細胞培養容器の培養面としてプラスチック材もしくはNIPPAmを用いた材料が使用されるが、下記は培養面を本発明の刺激応答材料で被覆して細胞の培養を行った。
「細胞のシート化培養」
(i)細胞及び培地
実験にはヒト口腔細胞(ScienCell社)およびヒト角膜細胞(Invitrogen社)を使用した。増殖用の培地はそれぞれの細胞に付属の専用培地(無血清培地)を用い,細胞の重層化では,共にKCMを用いた。
(ii)培養方法
ヒト口腔細胞又はヒト角膜細胞を、刺激応答性材料で被覆した6ウェルプレートに播き、上記同位体グルコースを含むDMEM/F12培地にてインキュベータ内(37℃、5%CO2、>95%湿度)で培養し、3日もしくは4日に1度培地交換を行い、コンフレントまで増殖した時点で分化誘導用培地(KCM培地)に交換して分化させた。分化誘導用培地に交換後3日もしくは4日に1度KCM培地にて培地交換を行うことで細胞を重層化させ、細胞シートを形成した。
(iii)細胞の回収
正常に培養された細胞の回収では、温度を37℃から20℃に下げることにより、細胞を回収した。
「培養評価」
pH6.8からpH7.6を維持した培地で培養した場合、細胞は剥離されず増殖した。一方、菌のコンタミによりpHが6.8より下回った際、細胞は培養面から剥離された。また、培地交換が遅れ、細胞が分泌する乳酸の蓄積により培養液のpHが6.8を下回った場合も同様に細胞が培養面から剥離した。
2…刺激応答材料(疎水性)
3…刺激応答材料(親水性)
4…培養プレート
Claims (11)
- pH応答性高分子よりなる領域と、前記と異なる温度応答性高分子よりなる領域を有し、前記領域が異なる位置にあることを特徴とする刺激応答型材料。
- ポリアミノ酸を骨格とし、その側鎖に疎水性の官能基で化学修飾されていること特徴とする請求項1に記載の刺激応答型材料。
- 前記温度応答性高分子がN−n−プロピルアクリルアミド、N−イソプロピルアクリルアミド、N−エチルアクリルアミド、N、N−ジメチルアクリルアミド、N−アクリロイルピロリジン、N−アクリロイルピペリジン、N−アクリロイルモルホリン、N−n−プロピルメタクリルアミド、N−イソプロピルメタクリルアミド、N−エチルメタクリルアミド、N、N−ジメチルメタクリルアミド、N−メタクリロイルピロリジン、N−メタクリロイルピペリジン及びN−メタクリロイルモルホリンから選ばれる少なくとも1種であることを特徴とする請求項1に記載の刺激応答型材料。
- 前記pH応答性高分子がカルボキシル基、リン酸、スルホニル及びアミノから選ばれる少なくとも1種であることを特徴とする請求項1に記載の刺激応答型材料。
- 前記pH応答性高分子が(メタ)アクリル酸、マレイン酸、スチレンスルホン酸、2−アクリルアミド−2−メチルプロパンスルホン酸、ホスホリルエチル(メタ)アクリレート、アミノエチルメタクリレート、アミノプロピル(メタ)アクリルアミド及びジメチルアミノプロピル(メタ)アクリルアミドから選ばれる少なくとも1種のモノマーが重合されたものであることを特徴とする請求項1に記載の刺激応答型材料。
- pH6.8以下で疎水性から親水性に変化することを特徴とする請求項1に記載の刺激応答型材料。
- pH7.6以上で疎水性から親水性に変化することを特徴とする請求項1に記載の刺激応答型材料。
- 温度が30℃以上で疎水性であり、30℃以下で親水性となることを特徴とする請求項1に記載の刺激応答型材料。
- 請求項1乃至8のいずれかの記載の刺激応答型材料の刺激応答型分子を培養面に固定化することを特徴とする細胞培養容器。
- 前記刺激応答型分子が共有結合により固定化されることを特徴とする請求項9に記載の細胞培養容器。
- 前記共有結合がアミド結合であること特徴とする請求項10に記載の細胞培養容器。
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WO2018037742A1 (ja) * | 2016-08-23 | 2018-03-01 | 日立化成株式会社 | 吸着材 |
JP2018050548A (ja) * | 2016-09-29 | 2018-04-05 | 住友ゴム工業株式会社 | がん細胞捕捉方法 |
JP2019129836A (ja) * | 2019-03-27 | 2019-08-08 | 住友ゴム工業株式会社 | がん細胞捕捉方法 |
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US11360078B2 (en) | 2016-09-29 | 2022-06-14 | Sumitomo Rubber Industries, Ltd. | Medical analysis device and cell analysis method |
US11573232B2 (en) | 2018-02-14 | 2023-02-07 | Sumitomo Rubber Industries, Ltd. | Method for capturing specific cells |
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US11614440B2 (en) | 2019-01-24 | 2023-03-28 | Sumitomo Rubber Industries, Ltd. | Specific cell fractionating and capturing methods |
JP2019129836A (ja) * | 2019-03-27 | 2019-08-08 | 住友ゴム工業株式会社 | がん細胞捕捉方法 |
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