EP3456348A1 - Making influenza virus vaccines without using eggs - Google Patents

Making influenza virus vaccines without using eggs Download PDF

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Publication number
EP3456348A1
EP3456348A1 EP18187826.5A EP18187826A EP3456348A1 EP 3456348 A1 EP3456348 A1 EP 3456348A1 EP 18187826 A EP18187826 A EP 18187826A EP 3456348 A1 EP3456348 A1 EP 3456348A1
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Prior art keywords
virus
influenza
influenza virus
mdck
vaccine
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German (de)
English (en)
French (fr)
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Theodore F. Tsai
Heidi Trusheim
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Seqirus UK Ltd
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Seqirus UK Ltd
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/12—Viral antigens
    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • A61K2039/5252—Virus inactivated (killed)
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    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16111—Influenzavirus A, i.e. influenza A virus
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    • C12N2760/16011—Orthomyxoviridae
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16234—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
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    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16211—Influenzavirus B, i.e. influenza B virus
    • C12N2760/16251—Methods of production or purification of viral material

Definitions

  • This invention is in the field of manufacturing vaccines for protecting against influenza virus.
  • the current process for preparing seasonal vaccines against human influenza virus infection involves the following steps [1,2]: (a) isolation of circulating virus strains; (b) antigenic and genetic analysis of isolated viruses; (c) selection of viral strains for use during the coming season; (d) preparation of high-growth seed strains by reassortment or the use of reverse genetics; (e) release of seed strains to vaccine manufacturers; (f) evaluation by the manufacturers of the strains' suitability for industrial production; and (g) growth of the seed strains to produce virus from which vaccines are then manufactured.
  • Steps (a) to (e) of this process are performed by the FDA and government-approved international influenza centres, typically under the auspices of the World Health Organisation; steps (f) and (g) are performed by the manufacturers themselves.
  • Step (d) transitions a virus from one that is naturally adapted for infecting humans into one that will grow to high titers under industrial growth conditions.
  • this step typically involves creating a 6:2 reassortant strain that includes the HA- and NA-encoding genome segments from the strains selected in (c) and the remaining six genome segments from a strain that grows efficiently in chicken eggs, and this strain is usually A/PR/8/34.
  • the reassortment procedure is then followed by repeated passaging of the strain in embryonated eggs to allow for egg adaptation and growth enhancement.
  • prototype strains with good growth characteristics are usually obtained by direct and repeated passaging in embryonated eggs without attempting to generate reassortants.
  • step (g) the steps performed prior to release to vaccine manufacturers involve passaging influenza virus through eggs. Even if the viruses are grown by a manufacturer in step (g) on a cell substrate, rather than on eggs, the virus will still have been passaged through eggs at some stage between isolation from in step (a) and receipt by a manufacturer in step (e).
  • step (a) involves exposing a substrate to a patient sample, such that any virus in the sample will infect the substrate.
  • the substrate can then amplify the amount of virus present, and the amplified viruses are then available for further study.
  • This step may take place in eggs or in mammalian cells.
  • Cells known for use in primary isolation include MRC-5 cells [3], Vero cells [4,5], MDCK cells [6], HepG2 cells [7], LLC-MK2 cells [8], etc .
  • chicken eggs continue to be used to isolate reference strains for the manufacture of influenza vaccine.
  • Reference 10 proposes that vaccines should be grown in cell culture using either a (i) a high growth strain of a passaged clinical isolate or (ii) a reassortant derived from at least one naturally-occurring mammalian influenza virus strain, provided that the isolate or reassortant has not been passaged in avian eggs.
  • a seed virus that has already been selected or manipulated for growth in the cell culture of choice.
  • Reference 11 compares viruses passaged through eggs with those passaged through MDCK cells, but specifically selects the former for vaccine manufacture.
  • Reference 12 suggests that seed viruses for pandemic influenza vaccines could be prepared by propagating the pandemic strain directly onto mammalian cell culture instead of via embryonated eggs, but notes that egg passaging was obligatory for inter-pandemic manufacture.
  • the reason for this obligatory egg passaging is that it has been believed to act as a 'filter' for adventitious agents: regulatory agencies have accepted that a series of passages in an avian system, between the original clinical isolation from a human and the final vaccine for administration to a human, will prevent mammalian-type adventitious agents from co-replicating with the influenza virus.
  • the invention aims to provide further procedures useful in manufacturing influenza vaccines, in which the use of eggs is reduced, and preferably is avoided altogether. In one particular aspect, the invention aims to provide further and improved procedures useful in influenza virus isolation.
  • a first aspect of the invention provides a process for preparing an influenza seed virus for vaccine manufacture, comprising steps of: (i) infecting a cell line with an influenza virus obtained either directly from a patient or from a primary isolate; (ii) passaging the virus from the infected cell line obtained in step (i) at least once; and (iii) culturing the infected cells from step (ii) in order to produce influenza virus.
  • Influenza virus purified from the culture of step (iii) can be used as the seed virus.
  • influenza virus used in step (i) is either an influenza B virus or is a non-pandemic influenza A virus i.e . at the current time is a H1N1 or H3N2 strain of influenza A.
  • steps (i), (ii) or (iii) involves growth or passaging of the virus in eggs.
  • the passaging in step (ii) will typically involve: allowing the influenza virus to replicate in cell culture; collecting replicated virus e.g . from the culture supernatant; and transferring the collected replicated virus to an uninfected cell culture. This process can be repeated. After at least one passage, the virus is allowed to replicate in step (iii) and virus is collected, but the virus is used as a seed virus rather than being transferred to an uninfected culture for further passaging.
  • the cell line used with the first aspect is preferably not a human cell line. By avoiding the use of human cells, the 'filtering' of adventitious agents can be maintained even without the use of eggs. Because of the close relationship to humans, the cell line is also preferably not a primate cell line e.g . it is not a Vero cell line (derived from monkey kidney). Reference 10 proposes the use of Vero cells during vaccine manufacture but these cells are permissive to many human viruses, and so any further human viruses that are present in the influenza virus sample used in step (i) will be able to grow in parallel with the influenza virus, leading to contamination of the eventual seed virus.
  • a preferred cell line for use with the first aspect of the invention is a canine cell line, such as the MDCK cell line (Madin Darby canine kidney), contrary to the specific teaching of reference 10. Further details of MDCK cells are given below. It has now been found that MDCK cells exhibit a 'filtering' effect against adventitious agents that is equivalent to that achieved by avian eggs. Based on this finding, therefore, MDCK cells can be used in place of eggs without increasing regulatory risk and, despite the reports in reference 13 that egg passaging on influenza virus conveys a growth advantage during MDCK culture, growth in eggs is avoided.
  • MDCK cell line Modin Darby canine kidney
  • Reference 14 discloses that influenza strains isolated from human patients, without any passages in eggs or cell culture, can grow efficiently in MDCK cell cultures, including in serum-free cultures.
  • the infection in step (i) may use an influenza virus from a clinical sample (e.g . from a pharyngeal swab, etc .) obtained directly from a patient, or it may use a virus which has already been subjected to primary isolation.
  • the primary isolation prior to step (i) may have taken place in eggs, but preferred primary isolates for use with the invention are those which were obtained without the use of eggs e.g. in mammalian cells.
  • Cells known for use in primary isolation include, but are not limited to, MRC-5 cells [3], Vero cells [4,5], MDCK cells [6], HepG2 cells [7], LLC-MK2 cells [8], etc .
  • step (i) where the invention uses primary isolates in step (i), it is preferred that primary isolation should have taken place in the same cell type as steps (i) to (iii).
  • MDCK is already known to be suitable for both primary isolation, passaging and growth of influenza viruses, but improvements in MDCK isolation are described below.
  • influenza virus isolate In order to maximise knowledge of the history of an influenza virus isolate, it is preferred to use a virus obtained directly from a clinical isolate rather than to use primary isolates.
  • Current influenza surveillance systems involve primary isolation in hospitals, with strains of interest being sent on to national and international influenza centres.
  • a portion In addition to using a patient sample for primary isolation, it is common for a portion to be set aside and stored ( e.g . by freezing) such that it is possible to return to the original material for re-isolation. Where such a stored sample is available then it can be used in step (i) in place of the primary isolate.
  • reverse genetics between steps (i) and (iii) in order to generate a new viral strain which has at least one viral genome segment from the parent virus.
  • Using reverse genetics between steps (i) and (iii) separates the viral products used in step (i) from the viral products used in step (iii), and so can act as a filter against adventitious agents that may have been introduced before step (i).
  • reverse genetics techniques can also be used for other reasons e.g . to generate reassortants, to manipulate coding sequences, to replace specific segments, etc . Further details are given below in relation to the second aspect of the invention.
  • a second aspect of the invention provides a process for preparing an influenza seed virus for vaccine manufacture, comprising steps of: (i) infecting a cell line with an influenza virus obtained either directly from a patient or from a primary isolate; (ii) preparing a cDNA of at least one viral RNA segment of an influenza virus produced by the infected cell line obtained in step (i), and using the cDNA in a reverse genetics procedure to prepare a new influenza virus having at least one viral RNA segment in common with the influenza virus of step (i); and (iii) infecting a cell line with the new influenza virus, and then culturing the cell line in order to produce the new influenza virus.
  • the virus used in step (i) may be an influenza A virus of any subtype, or may be an influenza B virus.
  • Preferred influenza A virus subtypes are H1, H3 and H5.
  • steps (i), (ii) or (iii) involves growth or passaging of the virus in eggs.
  • they Preferably they all take place in the same type of cell e.g . they all take place in MDCK cells.
  • the genome segment(s) transferred in step (ii) will include the HA segment, and may include the NA segment and/or one or more further segments.
  • the first and second aspects of the invention provide seed viruses. These seed viruses can be used in various ways.
  • Seed viruses can be characterised e.g . to sequence their nucleic acids and/or proteins, to check their antigenic relatedness to other strains ( e.g . circulating strains), to check their immunogenicity, etc . Sequencing the viral HA gene to reveal HA amino acid sequence is typical.
  • Seed viruses can be used to elicit anti-sera.
  • Seed viruses can be distributed to vaccine manufacturers.
  • Seed viruses can be stored for future use.
  • Seed viruses can be used to prepare working seed lots. This system allows safe storage of the original seed virus while day-to-day use is performed with the working seed lots. Working seeds may be frozen until required.
  • the preparation of a working seed lot may involve a step of viral growth in cell culture, preferably on the same cell type as used in preparation of the seed virus. Growth in eggs is not used for preparing working seed lots.
  • Seed viruses can be used to infect cell lines for growth to provide viruses for vaccine manufacture or for use in preparing diagnostic tests.
  • Seed viruses of the invention share many of the characteristics of current egg-derived seed viruses, but can differ in various ways.
  • preferred influenza A seed viruses of the invention include fewer than 6 (i.e . 0, 1, 2, 3, 4 or 5) viral segments from a PR/8/34 influenza virus, as described in more detail below.
  • a third aspect of the invention provides a process for preparing an influenza virus for vaccine manufacture, comprising steps of: (i) obtaining an influenza virus that is either circulating in the population or has a hemagglutinin that is antigenically representative of an influenza virus that is circulating in the population; (ii) infecting a cell line with the influenza virus obtained in step (i); (iii) passaging virus obtained from the infected cell line in step (ii) at least once, to give a seed strain; and (iv) culturing the seed strain from step (iii) in order to produce influenza virus.
  • a fourth aspect of the invention provides a process for preparing an influenza virus for vaccine manufacture, comprising steps of: (i) obtaining an influenza virus that is either circulating in the population or has a hemagglutinin that is antigenically representative of an influenza virus that is circulating in the population; (ii) infecting a cell line with the influenza virus obtained in step (i); (iii) preparing a cDNA of at least one viral RNA segment of an influenza virus produced by the infected cell line obtained in step (i), and using the cDNA in a reverse genetics procedure to prepare a influenza seed virus having at least one viral RNA segment in common with the influenza virus of step (i); and (iv) infecting a cell line with the influenza seed virus, and then culturing the passaged cell line from step (iii) in order to produce influenza virus.
  • the invention also provides a process for preparing an influenza virus vaccine, comprising these steps (i) to (iv) of the third or fourth aspect, followed by step: (v) treating virus obtained in step (iv) to give a vaccine. Details of techniques used in step (v) are given below.
  • the virus used in step (i) may be an influenza A virus of any subtype, or may be an influenza B virus.
  • Preferred influenza A virus subtypes are H1, H3 and H5 e.g. a H1N1 or H3N2.
  • the virus used in step (i) has not been propagated on eggs since its primary isolation, and preferably is not propagated on eggs at any stage between the patient who originally presented the virus and the beginning of step (i).
  • antigenically representative is used in the influenza vaccine art to describe viral strains which may not actually be in widespread circulation within the population, but which elicit immune responses that can protect against strains that are in circulation while being convenient for manufacturing purposes. Sera elicited by an "antigenically representative" strain will be able to inhibit a circulating strain e.g . in a hemagglutination inhibition assay.
  • Step (i) may involve starting with a number of different strains and then selecting a strain for further use. For instance, it may involve starting with a number of different H1N1 strains of influenza A virus and then selecting a strain for further use. It may involve starting with a number of different H3N2 strains of influenza A virus and then selecting a strain for further use. It may involve starting with a number of different strains of influenza B virus and then selecting a strain for further use. The selection will be based on the immunological and serological criteria that are routinely used when selecting strains for inclusion in influenza viruses e.g . to choose a strain that is antigenically representative of the most common and/or pathogenic strains in circulation.
  • Step (iii) may be followed be a step in which the seed virus is confirmed as being antigenically representative of the strain obtained in step (i). This verification step may be performed before step (iv) begins, or may be performed in parallel to step (iv).
  • the invention also provides a process for preparing an influenza virus vaccine, comprising steps of: (a) obtaining a virus that has been prepared by a method comprising either steps (i) to (iii) of the first aspect or steps (i) to (iv) of the second aspect; and (b) treating this virus to give a vaccine.
  • the third and fourth aspects of the invention allow the production of influenza vaccines from a patient sample (or a primary isolate), where the influenza virus used to prepare the vaccine is not passaged through egg at any stage.
  • a fifth aspect of the invention provides a process for preparing a reassortant influenza virus, comprising steps of: (i) infecting a cell line with both a first strain of influenza virus having a first set of genome segments and a second strain of influenza virus having a second set of genome segments, wherein the first strain has a HA segment encoding a desired hemagglutinin; and (ii) culturing the infected cells from step (i) in order to produce influenza virus having at least one segment from the first set of genome segments and at least one segment from the second set of genome segments, provided that said at least one segment from the first set of genome segments includes the HA segment from the first strain.
  • the process can transfer at least the HA segment from the first strain to the second strain, thereby creating a new reassortant strain with a different collection of viral genome segments from either the first or the second strains, without using eggs.
  • Influenza virus purified from the culture of step (ii) can be used as described elsewhere herein.
  • the reassortants may show improved growth characteristics relative to the first strain.
  • the reassortant may also include the NA segment from the first strain, encoding a desired neuraminidase.
  • the reassortant will generally include segments from the first strain and the second strain in a ratio of 1:7, 2:6, 3:5, 4:4, 5:3, 6:2 or 7:1. Having a majority of strains from the second strain is typical.
  • This process may be used to generate reassortants of influenza A virus and influenza B virus.
  • influenza A virus the second strain in some embodiments will be PR/8/34, but it is also possible to use other strains, including those which share only 1, 2, 3, 4 or 5 of segments NP, M, NS, PA, PB1 or PB2 in common with PR/8/34.
  • the invention also provides an influenza virus obtainable by a reassortment method of the fifth aspect.
  • the invention also provides the use of such a virus in vaccine manufacture.
  • steps (i) or (ii) involves growth, reassortment or passaging of the virus in eggs.
  • the reassortment process can conveniently be performed in the same cell types (e.g . MDCK cells) as used for isolation and passaging, as described elsewhere herein.
  • the first strain may conveniently be an influenza virus obtained either directly from a patient or from a primary isolate.
  • a sixth aspect of the invention uses MDCK cells.
  • the MDCK cells are grown in suspension.
  • the MDCK cells are grown in a serum-free medium or a protein-free medium.
  • the MDCK cells are non-tumorigenic.
  • the MDCK cells are not grown in the presence of an overlay medium.
  • the invention provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a MDCK cell, wherein the MDCK cell is growing in a suspension culture.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a MDCK cell, wherein the MDCK cell is growing in a serum-free medium.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a MDCK cell, wherein the MDCK cell is growing in a protein-free medium.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a non-tumorigenic MDCK cell.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a MDCK cell, wherein the MDCK cell is not provided with an overlay medium.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a MDCK cell, wherein the MDCK cell is growing in a serum-free suspension culture.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a MDCK cell, wherein the MDCK cell is growing in a protein-free suspension culture.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a non-tumorigenic MDCK cell, wherein the MDCK cell is growing in a suspension culture.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a non-tumorigenic MDCK cell, wherein the MDCK cell is growing in a serum-free suspension culture.
  • the invention also provides a method for isolating an influenza virus from a patient sample, comprising a step in which the patient sample is incubated with a non-tumorigenic MDCK cell, wherein the MDCK cell is growing in a protein-free suspension culture.
  • the invention also provides an influenza virus isolated by one of these methods, The invention also provides the use of such a virus in vaccine manufacture.
  • Incubation of the patient sample and the MDCK cell generally results in infection of the MDCK cell by an influenza virus, such as a human influenza virus, and in particular a human influenza A virus.
  • influenza virus such as a human influenza virus, and in particular a human influenza A virus.
  • the virus can replicate in the cells, and the replicated virus can then be collected.
  • it can be used in downstream method steps e.g . detection, characterisation, analysis, preparation of seed virus, manipulation, etc .
  • a virus may also be passaged and/or grown in MDCK cells.
  • a virus may also be passaged and/or grown in non-MDCK cells, or in eggs, or in another substrate.
  • the sixth aspect of the invention involves the use of MDCK cell lines.
  • the original MDCK cell line is available from the ATCC, but the sixth aspect of the invention uses derivatives of this cell line. As shown below, isolation in these derivatives has been shown to be superior to isolation in the original MDCK cell line. Suitable MDCK cells and their characteristics are discussed in more detail below.
  • some embodiments of the sixth aspect use a MDCK cell line that can spontaneously replicate in suspension culture.
  • Reference 30 discloses a MDCK cell line that was adapted for growth in suspension culture, and this cell line ('MDCK 33016') is particularly useful for the methods of the sixth aspect.
  • MDCK 33016 can grow in serum-free culture, and can grow without needing an overlay medium.
  • Another MDCK cell line that can grow in suspension culture, including in serum-free culture is the 'B-702' cell line [36; see below].
  • Non-tumorigenic MDCK cell lines for use with the sixth aspect include those disclosed in reference 37, such as 'MDCK-S', 'MDCK-SF101', 'MDCK-SF102' and 'MDCK-SF103' (see below).
  • the MDCK cells grow in serum-free culture media and/or protein free media.
  • the sixth aspect of the invention can avoid the need to use an overlay medium during influenza virus isolation.
  • a virus is not grown in eggs before being exposed to MDCK cells as described above. It may also be preferred that virus grown in MDCK cells, as described above, is not subsequently grown in eggs.
  • clinical samples used in influenza virus isolation can take various forms, but typically comprise respiratory secretions, including but not limited to: direct aspirates; gargles; nasal washings; nasal swabs; throat swabs; pharyngeal swabs; etc . These are generally taken from a patient suspected of having an influenza virus infection, including patients who may be harbouring a new strain of influenza virus.
  • Influenza viruses isolated by the methods of the sixth aspect may be used to prepare an influenza seed virus for vaccine manufacture.
  • a method of the sixth aspect may include a further step of passaging the virus from an infected MDCK cell line at least once.
  • the method may then include a step of culturing the infected cells in order to produce influenza virus.
  • Influenza virus purified after this culture step may be used as a seed virus, as described elsewhere herein.
  • a virus isolated according to the sixth aspect may also be used as a source for reverse genetics techniques.
  • cDNA may be prepared from at least one viral RNA segment of an influenza virus isolated according to the sixth aspect.
  • the cDNA may then be used in a reverse genetics procedure to prepare a new influenza virus having at least one viral RNA segment in common with the isolated influenza virus. This new influenza virus may then be used to infect a cell line e.g . for further culture.
  • the sixth aspect of the invention may be used to isolate any suitable influenza virus, including human influenza viruses. These may be influenza A viruses, influenza B viruses or influenza C viruses. Influenza A viruses are typical, and useful influenza A virus subtypes are H1, H3 and H5.
  • vaccines typically include two influenza A strains (H1N1 and H3N2) and one influenza B strain.
  • the sixth aspect may be used to isolate such strains, or to isolate pandemic viral strains, such as H2, H5, H7 or H9 subtype strains.
  • the sixth aspect may be used to isolate an influenza A virus having one of HA subtypes H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15 or H16.
  • Influenza viruses isolated according to the sixth aspect of the invention may include hemagglutinin with a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide.
  • the isolation methods of the invention have been found to assist in stable retention of a virus's HA sequence, and thus its oligosaccharide preference.
  • Viruses isolated according to the sixth aspect may be used in vaccine manufacture and in methods of treatment.
  • the invention also provides the use of an antigen prepared from a virus isolated according to the sixth aspect, in the manufacture of a medicament for raising an immune response in a patient.
  • Human influenza viruses bind to receptor oligosaccharides having a Sia( ⁇ 2,6)Gal terminal disaccharide (sialic acid linked ⁇ -2,6 to galactose), but eggs instead have receptor oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide. Growth of human influenza viruses in eggs provides selection pressure on hemagglutinin away from Sia( ⁇ 2,6)Gal binding towards Sia( ⁇ 2,3)Gal binding.
  • Vero cells Like eggs, Vero cells express predominantly Sia( ⁇ 2,3)Gal receptors [15]. In contrast, MDCK cells and PER.C6 cells express both Sia( ⁇ 2,3)Gal and Sia( ⁇ 2,6)Gal. Reference 16 reports transfection of MDCK cells to overexpress ⁇ -2,6-sialyltransferase in order to favour selection of Sia( ⁇ 2,6)Gal binding. Even without such manipulations, however, it is possible to grow influenza viruses on MDCK cells without shifting them towards Sia( ⁇ 2,3)Gal binding.
  • the invention can use cells that express both Sia( ⁇ 2,3)Gal and Sia( ⁇ 2,6)Gal, but can produce influenza viruses that have a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide.
  • influenza viruses used for infection in step (i) have a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide. This binding preference is retained during step (ii) and step (iii), such that the influenza virus produced in step (iii) has a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide.
  • influenza viruses used for infection in step (ii) have a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide. This binding preference is retained during step (iii) and step (iv), such that the influenza virus produced in step (iv) has a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide.
  • reference 17 describes a solid-phase enzyme-linked assay for influenza virus receptor-binding activity which gives sensitive and quantitative measurements of affinity constants.
  • Reference 18 used a solid-phase assay in which binding of viruses to two different sialylglycoproteins was assessed (ovomucoid, with Sia( ⁇ 2,3)Gal determinants; and pig ⁇ 2 -macroglobulin, which Sia( ⁇ 2,6)Gal determinants), and also describes an assay in which the binding of virus was assessed against two receptor analogs: free sialic acid (Neu5Ac) and 3'-sialyllactose (Neu5Ac ⁇ 2-3Gal ⁇ 1-4Glc).
  • Reference 19 reports an assay using a glycan array which was able to clearly differentiate receptor preferences for ⁇ 2,3 or ⁇ 2,6 linkages.
  • Reference 20 reports an assay based on agglutination of human erythrocytes enzymatically modified to contain either Sia( ⁇ 2,6)Gal or Sia( ⁇ 2,3)Gal. Depending on the type of assay, it may be performed directly with the virus itself, or can be performed indirectly with hemagglutinin purified from the virus.
  • the current process for manufacturing influenza viruses involves the preparation of reference reagents for each strain, namely (i) an anti-HA sera and (ii) purified whole virions. These calibrated reagents are used in a SRID assay to determine the level of HA in bulk antigens produced by manufacturers, thereby allowing them to dilute the bulks to give vaccines with the desired amount of HA per dose.
  • the invention provides reference materials based on viruses that have not been adapted to egg-based growth.
  • the invention provides a process for preparing an antiserum from an animal, comprising steps of: (i) administering to the animal a purified influenza virus hemagglutinin; and then (ii) recovering from the animal serum containing antibodies that recognise the hemagglutinin, characterised in that the hemagglutinin used in step (i) is from a virus grown in a cell line.
  • the hemagglutinin used in step (i) is preferably from a virus that has never been grown in eggs.
  • the hemagglutinin may have a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide.
  • Preferred hemagglutinin used to raise the antiserum is glycosylated with glycans obtainable from growth in a mammalian cell line (e.g . the cell lines described herein), such as MDCK.
  • Antisera may be generated for influenza A viruses and influenza B viruses.
  • the animal is preferably a mammal, such as a goat or more preferably a sheep.
  • Antisera can be conveniently prepared in sheep by extracting HA from purified virus by treatment with bromelain, followed by purification by sedimentation on a sucrose gradient.
  • a dose of about 50 ⁇ g hemagglutinin is administered intramuscularly to a sheep in combination with Freund's complete adjuvant (FCA).
  • FCA Freund's complete adjuvant
  • a 10 ⁇ g dose can be given two weeks later, and then 2-4 further doses at weekly intervals.
  • serum can be collected. Prior to use, it may be diluted (e.g . with PBS buffer containing sodium azide) and filled into containers.
  • the serum may be exposed to an acidic pH (e.g . pH 5 for two hours) in order to meet foot and mouth disease regulations.
  • the invention also provides a process for preparing an antiserum from an animal, comprising steps of: (i) growing influenza virus in a cell line; (ii) purifying hemagglutinin antigen from virus grown in step (i); (iii) administering the purified hemagglutinin from step (ii) to the animal; and then (iv) recovering from the animal serum containing antibodies that recognise the hemagglutinin.
  • the invention also provides antiserum obtainable by these processes.
  • the invention also provides a gel including this antiserum.
  • a process as described above for preparing an antiserum from an animal may include the further step of mixing the antiserum with a gel.
  • the gel is suitable for performing a SRID assay e.g. it is an agarose gel.
  • the invention provides antigen reference materials.
  • the invention provides a process for preparing an antigen reference material, comprising steps of: (i) growing influenza virus in a cell line; (ii) purifying viruses grown in step (i); and (iii) inactivating the virus, characterised in that the influenza virus used in step (i) has never been grown in eggs.
  • the process may include a further step of: (iv) lyophilising the inactivated virus.
  • the virus used in step (i) has never been grown in eggs.
  • its hemagglutinin may have a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide.
  • the reference material is free from egg-derived materials (e.g . free from ovalbumin, free from ovomucoid, free from chicken DNA). Glycoproteins in the reference material will be glycosylated with glycans obtainable from growth in a mammalian cell line ( e.g . the cell lines described herein), such as MDCK. Reference materials may be generated for influenza A viruses and influenza B viruses.
  • Reference materials are usually used in pairs, and so the invention also provides a kit comprising: (i) antiserum obtainable by these processes and (ii) antigen reference material obtainable by these processes.
  • the invention also provides a process for preparing the kit, comprising the steps of: (i) making an antiserum as described above; (ii) making an antigen reference material as described above; and (iii) combining the products of steps (i) and (ii) into a kit.
  • the antigen and antiserum are suitable and intended for use in SRID assays, and the invention provides a single radial immunodiffusion assay for influenza virus hemagglutinin, characterised in that the assay uses antiserum obtainable by these processes and/or antigen reference material obtainable by these processes.
  • the SRID assay will involve steps of preparing a gel including the antiserum, applying the antigen reference material (reconstituted, where necessary, in an aqueous medium) to the gel (typically into a well), and then permitting the antigen to diffuse radially into the gel.
  • the antigen may be treated with a detergent, such as a Zwittergent detergent, prior to use.
  • Viruses prepared or isolated by techniques of the invention
  • Preferred influenza A viruses of the invention include fewer than 6 (i.e. 0, 1, 2, 3, 4 or 5) viral segments from a PR/8/34 influenza virus. Preferably they include no PR/8/34 segments. If any PR/8/34 segment(s) is/are present then this/these will not include the PR/8/34 HA segment and usually will not include the PR/8/34 NA segment. Thus preferred viruses are those in which at least one of segments NP, M, NS, PA, PB1 and/or PB2 is not derived from PR/8/34.
  • At least one of segments NP, M, PA, PB1 and/or PB2 is not derived from PR/8/34.
  • the invention can improve over existing vaccines by adding to the normal HA and NA antigens one or more further epitope-containing antigens that is/are representative of a circulating strain.
  • preferred influenza A viruses include fewer than 6 (i.e . 0, 1, 2, 3, 4 or 5) viral segments from an AA/6/60 influenza virus (A/Ann Arbor/6/60). Preferably they include no AA/6/60 segments. If any AA/6/60 segment(s) is/are present then this/these will not include the AA/6/60 HA segment and usually will not include the AA/6/60 NA segment. Thus preferred viruses are those in which at least one of segments NP, M, NS, PA, PB1 and/or PB2 is not derived from AA/6/60. More preferably, at least one of segments NP, M, PA, PB1 and/or PB2 is not derived from AA/6/60.
  • Preferred influenza B viruses include fewer than 6 ( i.e. 0, 1, 2, 3, 4 or 5) viral segments from an AA/1/66 influenza virus (B/Ann Arbor/1/66). Preferably they include no AA/1/66 segments. If any AA/1/66 segment(s) is/are present then this/these will not include the AA/1/66 HA segment and usually will not include the AA/1/66 NA segment. Thus preferred viruses are those in which at least one of segments NP, M, NS, PA, PB1 and/or PB2 is not derived from AA/1/66. More preferably, at least one of segments NP, M, PA, PB1 and/or PB2 is not derived from AA/1/66.
  • Preferred influenza viruses of the invention include hemagglutinin with a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide. This binding preference is discussed in more detail above.
  • Preferred influenza viruses of the invention include seed viruses, viruses isolated from patient samples using MDCK cells, reassortant viruses, etc .
  • glycoproteins including hemagglutinin
  • the glycoproteins will comprise glycoforms that are not seen in viruses grown in chicken eggs e.g . they may have non-avian sugar linkages, including mammalian sugar linkages.
  • the invention involves the use of cell lines that support influenza virus replication, and avoids the use of eggs.
  • the cell line will typically be of mammalian origin. Suitable mammalian cells of origin include, but are not limited to, hamster, cattle, primate (including humans and monkeys) and dog cells, although the use of primate cells is not preferred. Various cell types may be used, such as kidney cells, fibroblasts, retinal cells, lung cells, etc . Examples of suitable hamster cells are the cell lines having the names BHK21 or HKCC. Suitable monkey cells are e.g . African green monkey cells, such as kidney cells as in the Vero cell line [21-23]. Suitable dog cells are e.g . kidney cells, as in the CLDK and MDCK cell lines.
  • suitable cell lines include, but are not limited to: MDCK; CHO; CLDK; HKCC; 293T; BHK; Vero; MRC-5; PER.C6 [24]; FRhL2; WI-38; etc .
  • Suitable cell lines are widely available e.g . from the American Type Cell Culture (ATCC) collection [25], from the Coriell Cell Repositories [26], or from the European Collection of Cell Cultures (ECACC).
  • ATCC American Type Cell Culture
  • ECACC European Collection of Cell Cultures
  • the ATCC supplies various different Vero cells under catalog numbers CCL-81, CCL-81.2, CRL-1586 and CRL-1587, and it supplies MDCK cells under catalog number CCL-34.
  • PER.C6 is available from the ECACC under deposit number 96022940. Any of these cell types can be used for growth, reassortment and/or passaging according to the invention.
  • the most preferred cell lines are those with mammalian-type glycosylation.
  • virus can be grown on avian cell lines [ e.g . refs. 27-29], including cell lines derived from ducks ( e.g . duck retina) or hens e.g. chicken embryo fibroblasts (CEF), etc ., but the use of mammalian cells means that vaccines can be free from avian DNA and egg proteins (such as ovalbumin and ovomucoid), thereby reducing allergenicity.
  • the most preferred cell lines for growing influenza viruses are MDCK cell lines [30-33], derived from Madin Darby canine kidney.
  • the original MDCK cell line is available from the ATCC as CCL-34, but derivatives of this cell line may also be used.
  • reference 30 discloses a MDCK cell line that was adapted for growth in suspension culture ('MDCK 33016' or '33016-PF', deposited as DSM ACC 2219; see also refs. 34 & 35).
  • reference 36 discloses a MDCK-derived cell line that grows in suspension in serum-free culture ('B-702', deposited as FERM BP-7449).
  • Reference 37 discloses non-tumorigenic MDCK cells, including 'MDCK-S' (ATCC PTA-6500), 'MDCK-SF101' (ATCC PTA-6501), 'MDCK-SF102' (ATCC PTA-6502) and 'MDCK-SF103' (ATCC PTA-6503).
  • Reference 38 discloses MDCK cell lines with high susceptibility to infection, including 'MDCK.5F1' cells (ATCC CRL-12042) Any of these MDCK cell lines can be used with the invention.
  • Virus may be grown on cells in adherent culture or in suspension. Microcarrier cultures can also be used. In some embodiments, the cells may thus be adapted for growth in suspension.
  • Cell lines are preferably grown in serum-free culture media and/or protein free media.
  • a medium is referred to as a serum-free medium when it has no additives from serum of human or animal origin.
  • the cells growing in such cultures naturally contain proteins themselves, but a protein-free medium is understood to mean one in which multiplication of the cells occurs with exclusion of (without addition to the culture medium of) proteins, growth factors, other protein additives and non-serum proteins, but can optionally include (in the culture medium) proteins such as trypsin or other proteases that may be necessary for viral growth.
  • Cell lines supporting influenza virus replication are preferably grown below 37°C [39] ( e.g . 30-36°C, or at about 30°C, 31°C, 32°C, 33°C, 34°C, 35°C, 36°C) during viral replication.
  • MDCK cells may be grown (before, during or after the isolation step) at these temperatures, particularly during viral replication.
  • Methods for propagating influenza virus in cultured cells generally includes the steps of inoculating a culture of cells with an inoculum of the strain to be grown, cultivating the infected cells for a desired time period for virus propagation, such as for example as determined by virus titer or antigen expression (e.g . between 24 and 168 hours after inoculation) and collecting the propagated virus.
  • the cultured cells are inoculated with a virus (measured by PFU or TCID 50 ) to cell ratio of 1:500 to 1:1, preferably 1:100 to 1:5, more preferably 1:50 to 1:10.
  • the virus is added to a suspension of the cells or is applied to a monolayer of the cells, and the virus is absorbed on the cells for at least 60 minutes but usually less than 300 minutes, preferably between 90 and 240 minutes at 25°C to 40°C, preferably 28°C to 37°C.
  • the infected cell culture e.g . monolayers
  • the harvested fluids are then either inactivated or stored frozen.
  • Cultured cells may be infected at a multiplicity of infection ("m.o.i.") of about 0.0001 to 10, preferably 0.002 to 5, more preferably to 0.001 to 2.
  • the cells are infected at a m.o.i of about 0.01.
  • Infected cells may be harvested 30 to 60 hours post infection.
  • the cells are harvested 34 to 48 hours post infection.
  • the cells are harvested 38 to 40 hours post infection.
  • Proteases typically trypsin
  • the proteases can be added at any suitable stage during the culture e.g . before inoculation, at the same time as inoculation, or after inoculation [39].
  • a cell line is not passaged from a master working cell bank beyond 40 population-doubling levels.
  • the viral inoculum and the viral culture are preferably free from ( i.e . will have been tested for and given a negative result for contamination by) herpes simplex virus, respiratory syncytial virus, parainfluenza virus 3, SARS coronavirus, adenovirus, rhinovirus, reoviruses, polyomaviruses, birnaviruses, circoviruses, and/or parvoviruses [40].
  • preferred MDCK cell lines used with the sixth aspect are free from ( i.e .
  • herpes simplex viruses will have been tested for and given a negative result for infection by) herpes simplex viruses, respiratory syncytial viruses, parainfluenza virus 3, SARS coronavirus, adenoviruses, rhinoviruses, reoviruses, polyomaviruses, birnaviruses, circoviruses, and/or parvoviruses. Absence of herpes simplex viruses is particularly preferred.
  • a MDCK cell line used with the invention preferably contains no marker for G418 resistance ( cf . reference 16). Thus the cell line may be sensitive to G418 treatment.
  • the cell line used with the invention preferably contains no exogenous plasmids ( cf . reference 16), except for any that may be required for reverse genetics techniques.
  • the invention can be used directly with clinical isolates or primary isolates.
  • the invention can be used with reassortant strains, including those generated using reverse genetic techniques [ e.g . 41-45].
  • Reverse genetics techniques can use in vitro manipulation of plasmids to generate combinations of viral segments, to facilitate manipulation of coding or non-coding sequences in the viral segments, to introduce mutations, etc.
  • the techniques can be used for both influenza A and influenza B viruses.
  • Reverse genetics typically involves expressing (a) DNA molecules that encode desired viral RNA molecules e.g . from poll promoters, bacterial RNA polymerase promoters, bacteriophage polymerase promoters, etc. and (b) DNA molecules that encode viral proteins e.g. from polII promoters, such that expression of both types of DNA in a cell leads to assembly of a complete intact infectious virion.
  • the DNA preferably provides all of the viral RNA and proteins, but it is also possible to use a helper virus to provide some of the RNA and proteins.
  • Plasmid-based methods using separate plasmids for producing each viral RNA are preferred [46-48], and these methods will also involve the use of plasmids to express all or some ( e.g . just PB1, PB2, PA & NP proteins) of the viral proteins, with 12 plasmids being used in some methods.
  • RNA polymerase I transcription cassettes for viral RNA synthesis
  • plasmid e.g . sequences encoding 1, 2, 3, 4, 5, 6, 7 or all 8 influenza A vRNA segments
  • protein-coding regions with RNA polymerase II promoters e.g . sequences encoding 1, 2, 3, 4, 5, 6, 7 or all 8 influenza A mRNA transcripts.
  • Preferred aspects of the reference 49 method involve: (a) PB1, PB2 and PA mRNA-encoding regions on a single plasmid; and (b) all 8 vRNA-encoding segments on a single plasmid. Including the NA and HA segments on one plasmid and the six other segments on another plasmid can also facilitate matters.
  • the canine polI promoter [50] may be used when performing reverse genetics in MDCK cells.
  • poll promoters it is possible to use bacteriophage polymerase promoters [51].
  • promoters for the SP6, T3 or T7 polymerases can conveniently be used.
  • bacteriophage polymerase promoters can be more convenient for many cell types (e.g . MDCK), although a cell must also be transfected with a plasmid encoding the exogenous polymerase enzyme.
  • strains used for growth in eggs usually include six RNA segments from a PR/8/34 influenza A virus (with HA and N segments being from a vaccine strain, i.e . a 6:2 reassortant), the avoidance of eggs with the invention means that PR/8/34 segments can be omitted.
  • Influenza A viruses can thus include fewer than 6 ( i.e. 0, 1, 2, 3, 4 or 5) viral segments from a PR/8/34 influenza virus.
  • preferred viruses are those in which at least one of segments NP, M, NS, PA, PB1 and/or PB2 is not derived from PR/8/34.
  • a virus may include a NS segment that originated in an avian influenza virus.
  • the invention uses reverse genetics, it allows a viral RNA segment from a source influenza virus to be transferred into the genome of a destination influenza virus. These two viruses will thus have at least one viral RNA segment in common.
  • the term "in common" here means an identical copy of the whole segment, but can also extend to mean a modified copy of the segment with modifications in the coding and/or non-coding regions. Where modifications are made in the coding-region, these will not substantially change the immunogenicity and/or activity of the encoded protein.
  • a HA segment may be manipulated around the HA1/HA2 cleavage site without changing its ability to elicit effective anti-HA antibodies when administered to a patient.
  • reverse genetics may be used to modify the natural HA of a virus isolated according to the sixth aspect e.g. to remove determinants (e.g . hyper-basic regions around the HA1/HA2 cleavage site) that cause a virus to be highly pathogenic in avian species.
  • determinants e.g . hyper-basic regions around the HA1/HA2 cleavage site
  • influenza virus vaccine Various forms of influenza virus vaccine are currently available (e.g . see chapters 17 & 18 of reference 54).
  • Vaccines are generally based either on live virus or on inactivated virus. Inactivated vaccines may be based on whole virions, 'split' virions, or on purified surface antigens. Influenza antigens can also be presented in the form of virosomes. The invention can used when manufacturing any of these types of vaccine.
  • Live viruses include MedImmune's FLUMISTTM product (trivalent live virus).
  • Vaccine is prepared by a process that comprises growing the virus on a suitable substrate and then purifying virions from virion-containing fluids. For example, the fluids may be clarified by centrifugation, and stabilized with buffer (e.g . containing sucrose, potassium phosphate, and monosodium glutamate).
  • buffer e.g . containing sucrose, potassium phosphate, and monosodium glutamate
  • the vaccine may comprise whole virion, split virion, or purified surface antigens (including hemagglutinin and, usually, also including neuraminidase).
  • Chemical means for inactivating a virus include treatment with an effective amount of one or more of the following agents: detergents, formaldehyde, ⁇ -propiolactone, methylene blue, psoralen, carboxyfullerene (C60), binary ethylamine, acetyl ethyleneimine, or combinations thereof.
  • Non-chemical methods of viral inactivation are known in the art, such as for example UV light or gamma irradiation.
  • Virions can be harvested from virus-containing fluids by various methods. For example, a purification process may involve zonal centrifugation using a linear sucrose gradient solution that includes detergent to disrupt the virions. Antigens may then be purified, after optional dilution, by diafiltration.
  • Split virions are obtained by treating purified virions with detergents (e.g . ethyl ether, polysorbate 80, deoxycholate, tri- N -butyl phosphate, Triton X-100, Triton N101, cetyltrimethylammonium bromide, Tergitol NP9, etc .) to produce subvirion preparations, including the 'Tween-ether' splitting process.
  • detergents e.g ethyl ether, polysorbate 80, deoxycholate, tri- N -butyl phosphate, Triton X-100, Triton N101, cetyltrimethylammonium bromide, Tergitol NP9, etc .
  • Methods of splitting influenza viruses are well known in the art e.g. see refs. 55-60, etc .
  • Splitting of the virus is typically carried out by disrupting or fragmenting whole virus, whether infectious or non-infectious with a disrupting concentration of a splitting agent
  • Preferred splitting agents are non-ionic and ionic (e.g . cationic) surfactants e.g. alkylglycosides, alkylthioglycosides, acyl sugars, sulphobetaines, betains, polyoxyethylenealkylethers, N,N-dialkyl-Glucamides, Hecameg, alkylphenoxy-polyethoxyethanols, NP9, quaternary ammonium compounds, sarcosyl, CTABs (cetyl trimethyl ammonium bromides), tri- N -butyl phosphate, Cetavlon, myristyltrimethylammonium salts, lipofectin, lipofectamine, and DOT-MA, the octyl- or nonylphenoxy polyoxyethanols (e.g .
  • non-ionic and ionic (e.g . cationic) surfactants e.g. alkylglycosides, alky
  • Triton surfactants such as Triton X-100 or Triton N101
  • polyoxyethylene sorbitan esters the Tween surfactants
  • polyoxyethylene ethers polyoxyethlene esters, etc .
  • One useful splitting procedure uses the consecutive effects of sodium deoxycholate and formaldehyde, and splitting can take place during initial virion purification (e.g. in a sucrose density gradient solution).
  • a splitting process can involve clarification of the virion-containing material (to remove non-virion material), concentration of the harvested virions (e.g .
  • split virions can usefully be resuspended in sodium phosphate-buffered isotonic sodium chloride solution.
  • the BEGRIVACTM, FLUARIXTM, FLUZONETM and FLUSHIELDTM products are split vaccines.
  • Purified surface antigen vaccines comprise the influenza surface antigens hemagglutinin and, typically, also neuraminidase. Processes for preparing these proteins in purified form are well known in the art.
  • the FLUVIRINTM, AGRIPPALTM and INFLUVACTM products are subunit vaccines.
  • Virosomes can be prepared by solubilization of influenza virus with a detergent followed by removal of the nucleocapsid and reconstitution of the membrane containing the viral glycoproteins.
  • An alternative method for preparing virosomes involves adding viral membraneglycoproteins to excess amounts of phospholipids, to give liposomes with viral proteins in their membrane.
  • the invention can be used to store bulk virosomes. as in the INFLEXAL VTM and INVAVACTM products.
  • the influenza virus may be attenuated.
  • the influenza virus may be temperature-sensitive.
  • the influenza virus may be cold-adapted. These three features are particularly useful when using live virus as an antigen.
  • HA is the main immunogen in current inactivated influenza vaccines, and vaccine doses are standardised by reference to HA levels, typically measured by SRID.
  • Existing vaccines typically contain about 15 ⁇ g of HA per strain, although lower doses can be used e.g. for children, or in pandemic situations, or when using an adjuvant.
  • Fractional doses such as 1 ⁇ 2 ( i.e . 7.5 ⁇ g HA per strain), 1 ⁇ 4 and 1 ⁇ 8 have been used [81,82], as have higher doses ( e.g . 3x or 9x doses [62,63]).
  • vaccines may include between 0.1 and 150 ⁇ g of HA per influenza strain, preferably between 0.1 and 50 ⁇ g e.g .
  • Particular doses include e.g . about 45, about 30, about 15, about 10, about 7.5, about 5, about 3.8, about 1.9, about 1.5, etc. per strain.
  • TCID 50 median tissue culture infectious dose
  • a TCID 50 of between 10 6 and 10 8 (preferably between 10 6.5 -10 7.5 ) per strain is typical.
  • Strains used with the invention may have a natural HA as found in a wild-type virus, or a modified HA. For instance, it is known to modify HA to remove determinants (e.g . hyper-basic regions around the HA1/HA2 cleavage site) that cause a virus to be highly pathogenic in avian species.
  • determinants e.g . hyper-basic regions around the HA1/HA2 cleavage site
  • Influenza virus strains for use in vaccines change from season to season.
  • vaccines typically include two influenza A strains (H1N1 and H3N2) and one influenza B strain, and trivalent vaccines are typical.
  • the invention may also use pandemic viral strains (i.e . strains to which the vaccine recipient and the general human population are immunologically naive, in particular of influenza A virus), such as H2, H5, H7 or H9 subtype strains, and influenza vaccines for pandemic strains may be monovalent or may be based on a normal trivalent vaccine supplemented by a pandemic strain.
  • the invention may protect against one or more of HA subtypes H1, H2, H3, H4, H5, H6, H7, H8, H9, H10, H11, H12, H13, H14, H15 or H16.
  • the invention may protect against one or more of influenza A virus NA subtypes N1, N2, N3, N4, N5, N6, N7, N8 or N9.
  • compositions of the invention are particularly useful for immunizing against pandemic strains.
  • the characteristics of an influenza strain that give it the potential to cause a pandemic outbreak are: (a) it contains a new hemagglutinin compared to the hemagglutinins in currently-circulating human strains, i.e . one that has not been evident in the human population for over a decade ( e.g. H2), or has not previously been seen at all in the human population ( e.g .
  • H5, H6 or H9 that have generally been found only in bird populations), such that the human population will be immunologically naive to the strain's hemagglutinin; (b) it is capable of being transmitted horizontally in the human population; and (c) it is pathogenic to humans.
  • a virus with H5 hemagglutinin type is preferred for immunizing against pandemic influenza, such as a H5N1 strain.
  • Other possible strains include H5N3, H9N2, H2N2, H7N1 and H7N7, and any other emerging potentially pandemic strains.
  • a virus may fall into HA clade 1, HA clade 1', HA clade 2 or HA clade 3 [64], with clades 1 and 3 being particularly relevant.
  • strains which are resistant to antiviral therapy e.g . resistant to oseltamivir [65] and/or zanamivir
  • compositions of the invention may thus include antigen(s) from one or more (e.g . 1, 2, 3, 4 or more) influenza virus strains, including influenza A virus and/or influenza B virus.
  • a vaccine includes more than one strain of influenza
  • the different strains are typically grown separately and are mixed after the viruses have been harvested and antigens have been prepared.
  • a process of the invention may include the step of mixing antigens from more than one influenza strain.
  • a trivalent vaccine is preferred, including antigens from two influenza A virus strains and one influenza B virus strain.
  • the compositions may include antigen from a single influenza A strain. In some embodiments, the compositions may include antigen from two influenza A strains, provided that these two strains are not H1N1 and H3N2. In some embodiments, the compositions may include antigen from more than two influenza A strains.
  • the invention provides a process for preparing an influenza virus antigen for use in a vaccine, comprising steps of: (i) receiving an influenza virus; (ii) infecting a cell line with this influenza virus; and (iii) culturing the infected cells from step (ii) in order to produce influenza virus.
  • Virus obtained in step (iii) can be used to prepare vaccines e.g. by methods involving inactivation, formulation, etc .
  • the influenza virus received in step (i) will have one or more of the following characteristics: (a) it has never been propagated on an egg substrate; (b) it was isolated in a MDCK cell, such as a MDCK 33016 cell and/or a MDCK cell growing in serum-free medium; (c) it has never been propagated on a substrate growing in a serum-containing medium; (d) it was generated using reverse genetics techniques; (e) it is an influenza A virus with fewer than 6 viral segments from a PR/8/34 influenza virus and/or fewer than 6 viral segments from an AA/6/60 influenza virus or it is an influenza B virus with fewer than 6 viral segments from an AA/1/66 influenza virus; (f) it includes hemagglutinin with a binding preference for oligosaccharides with a Sia( ⁇ 2,6)Gal terminal disaccharide compared to oligosaccharides with a Sia( ⁇ 2,3)Gal terminal disaccharide; and/or (g) it has glycoproteins (including
  • a vaccine composition prepared according to the invention preferably contains less than lOng (preferably less than 1ng, and more preferably less than 100pg) of residual host cell DNA per dose, although trace amounts of host cell DNA may be present.
  • Vaccines containing ⁇ 10ng (e.g. ⁇ 1ng, ⁇ 100pg) host cell DNA per 15 ⁇ g of hemagglutinin are preferred, as are vaccines containing ⁇ 10ng ( e.g. ⁇ 1ng, ⁇ 100pg) host cell DNA per 0.25ml volume.
  • Vaccines containing ⁇ 10ng ( e.g. ⁇ 1ng, ⁇ 100pg) host cell DNA per 50 ⁇ g of hemagglutinin are more preferred, as are vaccines containing ⁇ 10ng ( e.g. ⁇ 1ng, ⁇ 100pg) host cell DNA per 0.5ml volume.
  • the average length of any residual host cell DNA is less than 500bp e.g. less than 400bp, less than 300bp, less than 200bp, less than 100bp, etc .
  • Contaminating DNA can be removed during vaccine preparation using standard purification procedures e.g. chromatography, etc . Removal of residual host cell DNA can be enhanced by nuclease treatment e.g . by using a DNase.
  • a convenient method for reducing host cell DNA contamination is disclosed in references 67 & 68, involving a two-step treatment, first using a DNase (e.g . Benzonase), which may be used during viral growth, and then a cationic detergent (e.g . CTAB), which may be used during virion disruption.
  • Treatment with an alkylating agent, such as ⁇ -propiolactone can also be used to remove host cell DNA, and advantageously may also be used to inactivate virions [69].
  • the assay used to measure DNA will typically be a validated assay [70,71].
  • the performance characteristics of a validated assay can be described in mathematical and quantifiable terms, and its possible sources of error will have been identified.
  • the assay will generally have been tested for characteristics such as accuracy, precision, specificity. Once an assay has been calibrated ( e.g . against known standard quantities of host cell DNA) and tested then quantitative DNA measurements can be routinely performed.
  • hybridization methods such as Southern blots or slot blots [72]
  • immunoassay methods such as the ThresholdTM System [73]
  • quantitative PCR [74].
  • a typical assay involves non-sequence-specific formation of a reaction complex between a biotinylated ssDNA binding protein, a urease-conjugated anti-ssDNA antibody, and DNA. All assay components are included in the complete Total DNA Assay Kit available from the manufacturer. Various commercial manufacturers offer quantitative PCR assays for detecting residual host cell DNA e.g. AppTecTM Laboratory Services, BioRelianceTM, Althea Technologies, etc . A comparison of a chemiluminescent hybridisation assay and the total DNA ThresholdTM system for measuring host cell DNA contamination of a human viral vaccine can be found in reference 75.
  • Vaccine compositions manufactured according to the invention are pharmaceutically acceptable. They usually include components in addition to influenza antigens e.g . they typically include one or more pharmaceutical carrier(s) and/or excipient(s). As described below, adjuvants may also be included. A thorough discussion of such components is available in reference 76.
  • Vaccine compositions will generally be in aqueous form.
  • Vaccine compositions may include preservatives such as thiomersal or 2-phenoxyethanol. It is preferred, however, that the vaccine should be substantially free from ( i.e . less than 5 ⁇ g/ml) mercurial material e.g . thiomersal-free [59,77]. Vaccines containing no mercury are more preferred. ⁇ -tocopherol succinate can be included as an alternative to mercurial compounds [59]. Preservative-free vaccines are particularly preferred.
  • a physiological salt such as a sodium salt.
  • Sodium chloride (NaCl) is preferred, which may be present at between 1 and 20 mg/ml.
  • Other salts that may be present include potassium chloride, potassium dihydrogen phosphate, disodium phosphate dehydrate, magnesium chloride, calcium chloride, etc .
  • Vaccine compositions will generally have an osmolality of between 200 mOsm/kg and 400 mOsm/kg, preferably between 240-360 mOsm/kg, and will more preferably fall within the range of 290-310 mOsm/kg. Osmolality has previously been reported not to have an impact on pain caused by vaccination [78], but keeping osmolality in this range is nevertheless preferred.
  • Vaccine compositions may include one or more buffers.
  • Typical buffers include: a phosphate buffer; a Tris buffer; a borate buffer; a succinate buffer; a histidine buffer (particularly with an aluminum hydroxide adjuvant); or a citrate buffer. Buffers will typically be included in the 5-20mM range.
  • the pH of a vaccine composition will generally be between 5.0 and 8.1, and more typically between 6.0 and 8.0 e.g. 6.5 and 7.5, or between 7.0 and 7.8.
  • a process of the invention may therefore include a step of adjusting the pH of the bulk vaccine prior to packaging.
  • the vaccine composition is preferably sterile.
  • the vaccine composition is preferably non-pyrogenic e.g . containing ⁇ 1 EU (endotoxin unit, a standard measure) per dose, and preferably ⁇ 0.1 EU per dose.
  • the vaccine composition is preferably gluten-free.
  • Vaccine compositions of the invention may include detergent e.g. a polyoxyethylene sorbitan ester surfactant (known as 'Tweens'), an octoxynol (such as octoxynol-9 (Triton X-100) or t-octylphenoxypolyethoxyethanol), a cetyl trimethyl ammonium bromide ('CTAB'), or sodium deoxycholate, particularly for a split or surface antigen vaccine.
  • the detergent may be present only at trace amounts.
  • the vaccine may included less than 1mg/ml of each of octoxynol-10 and polysorbate 80.
  • Other residual components in trace amounts could be antibiotics (e.g . neomycin, kanamycin, polymyxin B).
  • a vaccine composition may include material for a single immunisation, or may include material for multiple immunisations (i.e . a 'multidose' kit).
  • the inclusion of a preservative is preferred in multidose arrangements.
  • the compositions may be contained in a container having an aseptic adaptor for removal of material.
  • Influenza vaccines are typically administered in a dosage volume of about 0.5ml, although a half dose (i.e . about 0.25ml) may be administered to children.
  • compositions and kits are preferably stored at between 2°C and 8°C. They should not be frozen. They should ideally be kept out of direct light.
  • compositions of the invention may advantageously include an adjuvant, which can function to enhance the immune responses (humoral and/or cellular) elicited in a patient who receives the composition.
  • an adjuvant which can function to enhance the immune responses (humoral and/or cellular) elicited in a patient who receives the composition.
  • the use of adjuvants with influenza vaccines has been described before. In references 79 & 80, aluminum hydroxide was used, and in reference 81, a mixture of aluminum hydroxide and aluminum phosphate was used. Reference 82 also described the use of aluminum salt adjuvants.
  • the FLUADTM product from Chiron Vaccines includes an oil-in-water emulsion.
  • Adjuvants that can be used with the invention include, but are not limited to:
  • Compositions may include two or more of said adjuvants.
  • they may advantageously include both an oil-in-water emulsion and a cytokine-inducing agent, as this combination improves the cytokine responses elicited by influenza vaccines, such as the interferon- ⁇ response, with the improvement being much greater than seen when either the emulsion or the agent is used on its own.
  • Antigens and adjuvants in a composition will typically be in admixture.
  • Oil-in-water emulsions have been found to be particularly suitable for use in adjuvanting influenza virus vaccines.
  • Various such emulsions are known, and they typically include at least one oil and at least one surfactant, with the oil(s) and surfactant(s) being biodegradable (metabolisable) and biocompatible.
  • the oil droplets in the emulsion are generally less than 5 ⁇ m in diameter, and may even have a sub-micron diameter, with these small sizes being achieved with a microfluidiser to provide stable emulsions. Droplets with a size less than 220nm are preferred as they can be subjected to filter sterilization.
  • the invention can be used with oils such as those from an animal (such as fish) or vegetable source.
  • Sources for vegetable oils include nuts, seeds and grains. Peanut oil, soybean oil, coconut oil, and olive oil, the most commonly available, exemplify the nut oils.
  • Jojoba oil can be used e.g . obtained from the jojoba bean. Seed oils include safflower oil, cottonseed oil, sunflower seed oil, sesame seed oil and the like. In the grain group, corn oil is the most readily available, but the oil of other cereal grains such as wheat, oats, rye, rice, teff, triticale and the like may also be used.
  • 6-10 carbon fatty acid esters of glycerol and 1,2-propanediol may be prepared by hydrolysis, separation and esterification of the appropriate materials starting from the nut and seed oils.
  • Fats and oils from mammalian milk are metabolizable and may therefore be used in the practice of this invention.
  • the procedures for separation, purification, saponification and other means necessary for obtaining pure oils from animal sources are well known in the art.
  • Most fish contain metabolizable oils which may be readily recovered. For example, cod liver oil, shark liver oils, and whale oil such as spermaceti exemplify several of the fish oils which may be used herein.
  • a number of branched chain oils are synthesized biochemically in 5-carbon isoprene units and are generally referred to as terpenoids.
  • Shark liver oil contains a branched, unsaturated terpenoids known as squalene, 2,6,10,15,19,23-hexamethyl-2,6,10,14,18,22-tetracosahexaene, which is particularly preferred herein.
  • Squalane the saturated analog to squalene
  • Fish oils, including squalene and squalane are readily available from commercial sources or may be obtained by methods known in the art. Other preferred oils are the tocopherols (see below). Mixtures of oils can be used.
  • Surfactants can be classified by their 'HLB' (hydrophile/lipophile balance). Preferred surfactants of the invention have a HLB of at least 10, preferably at least 15, and more preferably at least 16.
  • the invention can be used with surfactants including, but not limited to: the polyoxyethylene sorbitan esters surfactants (commonly referred to as the Tweens), especially polysorbate 20 and polysorbate 80; copolymers of ethylene oxide (EO), propylene oxide (PO), and/or butylene oxide (BO), sold under the DOWFAXTM tradename, such as linear EO/PO block copolymers; octoxynols, which can vary in the number of repeating ethoxy (oxy-1,2-ethanediyl) groups, with octoxynol-9 (Triton X-100, or t-octylphenoxypolyethoxyethanol) being of particular interest; (octylphenoxy)polyethoxy
  • Non-ionic surfactants are preferred.
  • Preferred surfactants for including in the emulsion are Tween 80 (polyoxyethylene sorbitan monooleate), Span 85 (sorbitan trioleate), lecithin and Triton X-100.
  • surfactants can be used e.g . Tween 80/Span 85 mixtures.
  • a combination of a polyoxyethylene sorbitan ester such as polyoxyethylene sorbitan monooleate (Tween 80) and an octoxynol such as t-octylphenoxypolyethoxyethanol (Triton X-100) is also suitable.
  • Another useful combination comprises laureth 9 plus a polyoxyethylene sorbitan ester and/or an octoxynol.
  • Preferred amounts of surfactants are: polyoxyethylene sorbitan esters (such as Tween 80) 0.01 to 1%, in particular about 0.1 %; octyl- or nonylphenoxy polyoxyethanols (such as Triton X-100, or other detergents in the Triton series) 0.001 to 0.1 %, in particular 0.005 to 0.02%; polyoxyethylene ethers (such as laureth 9) 0.1 to 20 %, preferably 0.1 to 10 % and in particular 0.1 to 1 % or about 0.5%.
  • polyoxyethylene sorbitan esters such as Tween 80
  • octyl- or nonylphenoxy polyoxyethanols such as Triton X-100, or other detergents in the Triton series
  • polyoxyethylene ethers such as laureth 9
  • oil-in-water emulsion adjuvants useful with the invention include, but are not limited to:
  • the emulsions may be mixed with antigen extemporaneously, at the time of delivery.
  • the adjuvant and antigen may be kept separately in a packaged or distributed vaccine, ready for final formulation at the time of use.
  • the antigen will generally be in an aqueous form, such that the vaccine is finally prepared by mixing two liquids.
  • the volume ratio of the two liquids for mixing can vary ( e.g . between 5:1 and 1:5) but is generally about 1:1.
  • hemagglutininin antigen will generally remain in aqueous solution but may distribute itself around the oil/water interface. In general, little if any hemagglutinin will enter the oil phase of the emulsion.
  • composition includes a tocopherol
  • any of the ⁇ , ⁇ , ⁇ , ⁇ , ⁇ or ⁇ tocopherols can be used, but ⁇ -tocopherols are preferred.
  • the tocopherol can take several forms e.g. different salts and/or isomers. Salts include organic salts, such as succinate, acetate, nicotinate, etc . D- ⁇ -tocopherol and DL- ⁇ -tocopherol can both be used.
  • Tocopherols are advantageously included in vaccines for use in elderly patients ( e.g . aged 60 years or older) because vitamin E has been reported to have a positive effect on the immune response in this patient group [122].
  • a preferred ⁇ -tocopherol is DL- ⁇ -tocopherol, and the preferred salt of this tocopherol is the succinate.
  • the succinate salt has been found to cooperate with TNF-related ligands in vivo .
  • ⁇ -tocopherol succinate is known to be compatible with influenza vaccines and to be a useful preservative as an alternative to mercurial compounds [59].
  • Cytokine-inducing agents for inclusion in compositions of the invention are able, when administered to a patient, to elicit the immune system to release cytokines, including interferons and interleukins. Cytokine responses are known to be involved in the early and decisive stages of host defense against influenza infection [124]. Preferred agents can elicit the release of one or more of: interferon- ⁇ ; interleukin-1; interleukin-2; interleukin-12; TNF- ⁇ ; TNF- ⁇ ; and GM-CSF. Preferred agents elicit the release of cytokines associated with a Th1-type immune response e.g . interferon- ⁇ , TNF- ⁇ , interleukin-2. Stimulation of both interferon-y and interleukin-2 is preferred.
  • a patient will have T cells that, when stimulated with an influenza antigen, will release the desired cytokine(s) in an antigen-specific manner.
  • T cells purified form their blood will release ⁇ -interferon when exposed in vitro to influenza virus hemagglutinin.
  • Methods for measuring such responses in peripheral blood mononuclear cells (PBMC) are known in the art, and include ELISA, ELISPOT, flow-cytometry and real-time PCR.
  • reference 125 reports a study in which antigen-specific T cell-mediated immune responses against tetanus toxoid, specifically ⁇ -interferon responses, were monitored, and found that ELISPOT was the most sensitive method to discriminate antigen-specific TT-induced responses from spontaneous responses, but that intracytoplasmic cytokine detection by flow cytometry was the most efficient method to detect re-stimulating effects.
  • Suitable cytokine-inducing agents include, but are not limited to:
  • the cytokine-inducing agents for use in the present invention may be modulators and/or agonists of Toll-Like Receptors (TLR).
  • TLR Toll-Like Receptors
  • they may be agonists of one or more of the human TLR1, TLR2, TLR3, TLR4, TLR7, TLR8, and/or TLR9 proteins.
  • Preferred agents are agonists of TLR7 (e.g . imidazoquinolines) and/or TLR9 (e.g . CpG oligonucleotides). These agents are useful for activating innate immunity pathways.
  • the cytokine-inducing agent can be added to the composition at various stages during its production. For example, it may be within an antigen composition, and this mixture can then be added to an oil-in-water emulsion. As an alternative, it may be within an oil-in-water emulsion, in which case the agent can either be added to the emulsion components before emulsification, or it can be added to the emulsion after emulsification. Similarly, the agent may be coacervated within the emulsion droplets.
  • the location and distribution of the cytokine-inducing agent within the final composition will depend on its hydrophilic/lipophilic properties e.g . the agent can be located in the aqueous phase, in the oil phase, and/or at the oil-water interface.
  • the cytokine-inducing agent can be conjugated to a separate agent, such as an antigen (e.g . CRM197).
  • a separate agent such as an antigen (e.g . CRM197).
  • an antigen e.g . CRM197
  • the adjuvants may be non-covalently associated with additional agents, such as by way of hydrophobic or ionic interactions.
  • Two preferred cytokine-inducing agents are (a) immunostimulatory oligonucleotides and (b) 3dMPL.
  • Immunostimulatory oligonucleotides can include nucleotide modifications/analogs such as phosphorothioate modifications and can be double-stranded or (except for RNA) single-stranded.
  • References 155, 156 and 157 disclose possible analog substitutions e.g . replacement of guanosine with 2'-deoxy-7-deazaguanosine.
  • the adjuvant effect of CpG oligonucleotides is further discussed in refs. 158-163.
  • a CpG sequence may be directed to TLR9, such as the motif GTCGTT or TTCGTT [164].
  • the CpG sequence may be specific for inducing a Th1 immune response, such as a CpG-A ODN (oligodeoxynucleotide), or it may be more specific for inducing a B cell response, such a CpG-B ODN.
  • CpG-A and CpG-B ODNs are discussed in refs. 165-167.
  • the CpG is a CpG-A ODN.
  • the CpG oligonucleotide is constructed so that the 5' end is accessible for receptor recognition.
  • two CpG oligonucleotide sequences may be attached at their 3' ends to form "immunomers". See, for example, references 164 & 168-170.
  • a useful CpG adjuvant is CpG7909, also known as ProMuneTM (Coley Pharmaceutical Group, Inc.).
  • TpG sequences can be used [171]. These oligonucleotides may be free from unmethylated CpG motifs.
  • the immunostimulatory oligonucleotide may be pyrimidine-rich.
  • it may comprise more than one consecutive thymidine nucleotide (e.g . TTTT, as disclosed in ref. 171), and/or it may have a nucleotide composition with >25% thymidine ( e.g . >35%, >40%, >50%, >60%, >80%, etc .).
  • it may comprise more than one consecutive cytosine nucleotide (e.g. CCCC, as disclosed in ref. 171), and/or it may have a nucleotide composition with >25% cytosine (e.g . >35%, >40%, >50%, >60%, >80%, etc .).
  • These oligonucleotides may be free from unmethylated CpG motifs.
  • Immunostimulatory oligonucleotides will typically comprise at least 20 nucleotides. They may comprise fewer than 100 nucleotides.
  • 3dMPL (also known as 3 de-O-acylated monophosphoryl lipid A or 3-O-desacyl-4'-monophosphoryl lipid A) is an adjuvant in which position 3 of the reducing end glucosamine in monophosphoryl lipid A has been de-acylated.
  • 3dMPL has been prepared from a heptoseless mutant of Salmonella minnesota, and is chemically similar to lipid A but lacks an acid-labile phosphoryl group and a base-labile acyl group. It activates cells of the monocyte/macrophage lineage and stimulates release of several cytokines, including IL-1, IL-12, TNF- ⁇ and GM-CSF (see also ref. 172). Preparation of 3dMPL was originally described in reference 173.
  • 3dMPL can take the form of a mixture of related molecules, varying by their acylation (e.g . having 3, 4, 5 or 6 acyl chains, which may be of different lengths).
  • the two glucosamine (also known as 2-deoxy-2-amino-glucose) monosaccharides are N-acylated at their 2-position carbons ( i.e . at positions 2 and 2'), and there is also O-acylation at the 3' position.
  • the group attached to carbon 2 has formula -NH-CO-CH 2 -CR 1 R 1 '.
  • the group attached to carbon 2' has formula -NH-CO-CH 2 -CR 2 R 2 .
  • the group attached to carbon 3' has formula -O-CO-CH 2 -CR 3 R 3' .
  • a representative structure is: Groups R 1 , R 2 and R 3 are each independently -(CH 2 ) n -CH 3 .
  • the value of n is preferably between 8 and 16, more preferably between 9 and 12, and is most preferably 10.
  • Groups R 1' , R 2' and R 3' can each independently be: (a) -H; (b) -OH; or (c) -O-CO-R 4 ,where R 4 is either -H or -(CH 2 ) m -CH 3 , wherein the value of m is preferably between 8 and 16, and is more preferably 10, 12 or 14. At the 2 position, m is preferably 14. At the 2' position, m is preferably 10. At the 3' position, m is preferably 12.
  • Groups R 1' , R 2' and R 3' are thus preferably -O-acyl groups from dodecanoic acid, tetradecanoic acid or hexadecanoic acid.
  • the 3dMPL has only 3 acyl chains (one on each of positions 2, 2' and 3').
  • the 3dMPL can have 4 acyl chains.
  • the 3dMPL can have 5 acyl chains.
  • the 3dMPL can have 6 acyl chains.
  • the 3dMPL adjuvant used according to the invention can be a mixture of these forms, with from 3 to 6 acyl chains, but it is preferred to include 3dMPL with 6 acyl chains in the mixture, and in particular to ensure that the hexaacyl chain form makes up at least 10% by weight of the total 3dMPL e.g . ⁇ 20%, ⁇ 30%, ⁇ 40%, ⁇ 50% or more. 3dMPL with 6 acyl chains has been found to be the most adjuvant-active form.
  • references to amounts or concentrations of 3dMPL in compositions of the invention refer to the combined 3dMPL species in the mixture.
  • 3dMPL can form micellar aggregates or particles with different sizes e.g . with a diameter ⁇ 150nm or >500nm. Either or both of these can be used with the invention, and the better particles can be selected by routine assay. Smaller particles (e.g . small enough to give a clear aqueous suspension of 3dMPL) are preferred for use according to the invention because of their superior activity [174]. Preferred particles have a mean diameter less than 220nm, more preferably less than 200nm or less than 150nm or less than 120nm, and can even have a mean diameter less than 100nm. In most cases, however, the mean diameter will not be lower than 50nm.
  • Particle diameter can be assessed by the routine technique of dynamic light scattering, which reveals a mean particle diameter. Where a particle is said to have a diameter of x nm, there will generally be a distribution of particles about this mean, but at least 50% by number ( e.g . ⁇ 60%, ⁇ 70%, ⁇ 80%, ⁇ 90%, or more) of the particles will have a diameter within the range x ⁇ 25 % .
  • 3dMPL can advantageously be used in combination with an oil-in-water emulsion. Substantially all of the 3dMPL may be located in the aqueous phase of the emulsion.
  • the 3dMPL can be used on its own, or in combination with one or more further compounds.
  • 3dMPL in combination with the QS21 saponin [175] (including in an oil-in-water emulsion [176]), with an immunostimulatory oligonucleotide, with both QS21 and an immunostimulatory oligonucleotide, with aluminum phosphate [177], with aluminum hydroxide [178], or with both aluminum phosphate and aluminum hydroxide.
  • Fatty adjuvants that can be used with the invention include the oil-in-water emulsions described above, and also include, for example:
  • the adjuvants known as aluminum hydroxide and aluminum phosphate may be used. These names are conventional, but are used for convenience only, as neither is a precise description of the actual chemical compound which is present ( e.g . see chapter 9 of reference 112).
  • the invention can use any of the "hydroxide” or "phosphate” adjuvants that are in general use as adjuvants.
  • aluminium hydroxide typically aluminium oxyhydroxide salts, which are usually at least partially crystalline.
  • Aluminium oxyhydroxide which can be represented by the formula AIO(OH)
  • IR infrared
  • the degree of crystallinity of an aluminium hydroxide adjuvant is reflected by the width of the diffraction band at half height (WHH), with poorly-crystalline particles showing greater line broadening due to smaller crystallite sizes.
  • aluminium hydroxide adjuvants The surface area increases as WHH increases, and adjuvants with higher WHH values have been seen to have greater capacity for antigen adsorption.
  • a fibrous morphology e.g . as seen in transmission electron micrographs
  • the pI of aluminium hydroxide adjuvants is typically about 11 i.e . the adjuvant itself has a positive surface charge at physiological pH.
  • Adsorptive capacities of between 1.8-2.6 mg protein per mg Al +++ at pH 7.4 have been reported for aluminium hydroxide adjuvants.
  • the adjuvants known as "aluminium phosphate” are typically aluminium hydroxyphosphates, often also containing a small amount of sulfate (i.e . aluminium hydroxyphosphate sulfate). They may be obtained by precipitation, and the reaction conditions and concentrations during precipitation influence the degree of substitution of phosphate for hydroxyl in the salt. Hydroxyphosphates generally have a PO 4 /Al molar ratio between 0.3 and 1.2. Hydroxyphosphates can be distinguished from strict AlPO 4 by the presence of hydroxyl groups. For example, an IR spectrum band at 3164cm -1 ( e.g . when heated to 200°C) indicates the presence of structural hydroxyls [ch.9 of ref. 112]
  • the PO 4 /Al 3+ molar ratio of an aluminium phosphate adjuvant will generally be between 0.3 and 1.2, preferably between 0.8 and 1.2, and more preferably 0.95+0.1.
  • the aluminium phosphate will generally be amorphous, particularly for hydroxyphosphate salts.
  • a typical adjuvant is amorphous aluminium hydroxyphosphate with PO 4 /Al molar ratio between 0.84 and 0.92, included at 0.6mg Al 3+ /ml.
  • the aluminium phosphate will generally be particulate ( e.g . plate-like morphology as seen in transmission electron micrographs). Typical diameters of the particles are in the range 0.5-20 ⁇ m ( e.g . about 5-10 ⁇ m) after any antigen adsorption.
  • Adsorptive capacities of between 0.7-1.5 mg protein per mg Al +++ at pH 7.4 have been reported for aluminium phosphate adjuvants.
  • Suspensions of aluminium salts used to prepare compositions of the invention may contain a buffer (e.g . a phosphate or a histidine or a Tris buffer), but this is not always necessary.
  • the suspensions are preferably sterile and pyrogen-free.
  • a suspension may include free aqueous phosphate ions e.g. present at a concentration between 1.0 and 20 mM, preferably between 5 and 15 mM, and more preferably about 10 mM.
  • the suspensions may also comprise sodium chloride.
  • the invention can use a mixture of both an aluminium hydroxide and an aluminium phosphate [81].
  • there may be more aluminium phosphate than hydroxide e.g. a weight ratio of at least 2:1 e.g. ⁇ 5:1, ⁇ 6:1, ⁇ 7:1, ⁇ 8:1, ⁇ 9:1, etc .
  • the concentration of Al +++ in a composition for administration to a patient is preferably less than 10mg/ml e.g. ⁇ 5 mg/ml, ⁇ 4 mg/ml, ⁇ 3 mg/ml, ⁇ 2 mg/ml, ⁇ 1 mg/ml, etc .
  • a preferred range is between 0.3 and 1mg/ml.
  • a maximum of 0.85mg/dose is preferred.
  • the adjuvant component may include one or more further adjuvant or immunostimulating agents.
  • additional components include, but are not limited to: a 3-O-deacylated monophosphoryl lipid A adjuvant ('3d-MPL'); and/or an oil-in-water emulsion.
  • Suitable containers for compositions of the invention include vials, syringes (e.g . disposable syringes), nasal sprays, etc. These containers should be sterile.
  • the vial is preferably made of a glass or plastic material.
  • the vial is preferably sterilized before the composition is added to it.
  • vials are preferably sealed with a latex-free stopper, and the absence of latex in all packaging material is preferred.
  • the vial may include a single dose of vaccine, or it may include more than one dose (a 'multidose' vial) e.g. 10 doses.
  • Preferred vials are made of colorless glass.
  • a vial can have a cap ( e.g . a Luer lock) adapted such that a pre-filled syringe can be inserted into the cap, the contents of the syringe can be expelled into the vial ( e.g . to reconstitute lyophilised material therein), and the contents of the vial can be removed back into the syringe.
  • a needle can then be attached and the composition can be administered to a patient.
  • the cap is preferably located inside a seal or cover, such that the seal or cover has to be removed before the cap can be accessed.
  • a vial may have a cap that permits aseptic removal of its contents, particularly for multidose vials.
  • the syringe may have a needle attached to it. If a needle is not attached, a separate needle may be supplied with the syringe for assembly and use. Such a needle may be sheathed. Safety needles are preferred. 1-inch 23-gauge, 1-inch 25-gauge and 5/8-inch 25-gauge needles are typical. Syringes may be provided with peel-off labels on which the lot number, influenza season and expiration date of the contents may be printed, to facilitate record keeping.
  • the plunger in the syringe preferably has a stopper to prevent the plunger from being accidentally removed during aspiration.
  • the syringes may have a latex rubber cap and/or plunger.
  • Disposable syringes contain a single dose of vaccine.
  • the syringe will generally have a tip cap to seal the tip prior to attachment of a needle, and the tip cap is preferably made of a butyl rubber. If the syringe and needle are packaged separately then the needle is preferably fitted with a butyl rubber shield.
  • Preferred syringes are those marketed under the trade name "Tip-Lok"TM.
  • Containers may be marked to show a half-dose volume e.g . to facilitate delivery to children.
  • a syringe containing a 0.5ml dose may have a mark showing a 0.25ml volume.
  • a glass container e.g . a syringe or a vial
  • a container made from a borosilicate glass rather than from a soda lime glass.
  • a kit or composition may be packaged ( e.g . in the same box) with a leaflet including details of the vaccine e.g. instructions for administration, details of the antigens within the vaccine, etc .
  • the instructions may also contain warnings e.g . to keep a solution of adrenaline readily available in case of anaphylactic reaction following vaccination, etc .
  • the invention provides a vaccine manufactured according to the invention.
  • Vaccine compositions manufactured according to the invention are suitable for administration to human patients, and the invention provides a method of raising an immune response in a patient, comprising the step of administering a composition of the invention to the patient.
  • the invention also provides a composition of the invention for use as a medicament.
  • the invention also provides the use of an influenza virus antigen prepared according to the invention, in the manufacture of a medicament for raising an immune response in a patient.
  • the immune response raised by these methods and uses will generally include an antibody response, preferably a protective antibody response.
  • Methods for assessing antibody responses, neutralising capability and protection after influenza virus vaccination are well known in the art. Human studies have shown that antibody titers against hemagglutinin of human influenza virus are correlated with protection (a serum sample hemagglutination-inhibition titer of about 30-40 gives around 50% protection from infection by a homologous virus) [185].
  • Antibody responses are typically measured by hemagglutination inhibition, by microneutralisation, by single radial immunodiffusion (SRID), and/or by single radial hemolysis (SRH). These assay techniques are well known in the art.
  • compositions of the invention can be administered in various ways.
  • the most preferred immunisation route is by intramuscular injection (e.g. into the arm or leg), but other available routes include subcutaneous injection, intranasal [186-188], oral [189], intradermal [190,191], transcutaneous, transdermal [192], etc .
  • Vaccines prepared according to the invention may be used to treat both children and adults. Influenza vaccines are currently recommended for use in pediatric and adult immunisation, from the age of 6 months. Thus the patient may be less than 1 year old, 1-5 years old, 5-15 years old, 15-55 years old, or at least 55 years old.
  • Preferred patients for receiving the vaccines are the elderly (e.g . ⁇ 50 years old, ⁇ 60 years old, and preferably ⁇ 65 years), the young ( e.g . ⁇ 5 years old), hospitalised patients, healthcare workers, armed service and military personnel, pregnant women, the chronically ill, immunodeficient patients, patients who have taken an antiviral compound (e.g .
  • an oseltamivir or zanamivir compound in the 7 days prior to receiving the vaccine, people with egg allergies and people travelling abroad.
  • the vaccines are not suitable solely for these groups, however, and may be used more generally in a population. For pandemic strains, administration to all age groups is preferred.
  • compositions of the invention satisfy 1, 2 or 3 of the CPMP criteria for efficacy.
  • these criteria are: (1) ⁇ 70% seroprotection; (2) ⁇ 40% seroconversion; and/or (3) a GMT increase of ⁇ 2.5-fold.
  • these criteria are: (1) ⁇ 60% seroprotection; (2) ⁇ 30% seroconversion; and/or (3) a GMT increase of ⁇ 2-fold.
  • Treatment can be by a single dose schedule or a multiple dose schedule. Multiple doses may be used in a primary immunisation schedule and/or in a booster immunisation schedule. In a multiple dose schedule the various doses may be given by the same or different routes e.g. a parenteral prime and mucosal boost, a mucosal prime and parenteral boost, etc . Administration of more than one dose (typically two doses) is particularly useful in immunologically naive patients e.g . for people who have never received an influenza vaccine before, or for vaccinating against a new HA subtype (as in a pandemic outbreak). Multiple doses will typically be administered at least 1 week apart ( e.g . about 2 weeks, about 3 weeks, about 4 weeks, about 6 weeks, about 8 weeks, about 10 weeks, about 12 weeks, about 16 weeks, etc .).
  • Vaccines produced by the invention may be administered to patients at substantially the same time as ( e.g . during the same medical consultation or visit to a healthcare professional or vaccination centre) other vaccines e.g . at substantially the same time as a measles vaccine, a mumps vaccine, a rubella vaccine, a MMR vaccine, a varicella vaccine, a MMRV vaccine, a diphtheria vaccine, a tetanus vaccine, a pertussis vaccine, a DTP vaccine, a conjugated H.influenzae type b vaccine, an inactivated poliovirus vaccine, a hepatitis B virus vaccine, a meningococcal conjugate vaccine (such as a tetravalent A-C-W135-Y vaccine), a respiratory syncytial virus vaccine, a pneumococcal conjugate vaccine, etc .
  • vaccines of the invention may be administered to patients at substantially the same time as ( e.g . during the same medical consultation or visit to a healthcare professional) an antiviral compound, and in particular an antiviral compound active against influenza virus (e.g . oseltamivir and/or zanamivir).
  • neuraminidase inhibitors such as a (3R,4R,5S)-4-acetylamino-5-amino-3(1-ethylpropoxy)-1-cyclohexene-1-carboxylic acid or 5-(acetylamino)-4-[(aminoiminomethyl)-amino]-2,6-anhydro-3,4,5-trideoxy-D-glycero-D-galactonon-2-enonic acid, including esters thereof (e.g . the ethyl esters) and salts thereof (e.g . the phosphate salts).
  • esters thereof e.g . the ethyl esters
  • salts thereof e.g . the phosphate salts
  • a preferred antiviral is (3R,4R,5S)-4-acetylamino-5-amino-3(1-ethylpropoxy)-1-cyclohexene-1-carboxylic acid, ethyl ester, phosphate (1:1), also known as oseltamivir phosphate (TAMIFLUTM).
  • composition comprising X may consist exclusively of X or may include something additional e.g . X + Y.
  • a process comprising a step of mixing two or more components does not require any specific order of mixing.
  • components can be mixed in any order. Where there are three components then two components can be combined with each other, and then the combination may be combined with the third component, etc .
  • animal (and particularly bovine) materials are used in the culture of cells, they should be obtained from sources that are free from transmissible spongiform encaphalopathies (TSEs), and in particular free from bovine spongiform encephalopathy (BSE). Overall, it is preferred to culture cells in the total absence of animal-derived materials.
  • TSEs transmissible spongiform encaphalopathies
  • BSE bovine spongiform encephalopathy
  • a compound is administered to the body as part of a composition then that compound may alternatively be replaced by a suitable prodrug.
  • Clinical specimens (nasal or throat swabs) containing influenza A and/or B virus subtypes were obtained from children and adults during the 2006-2007 northern hemisphere influenza season.
  • the susceptibility and reliability of the MDCK 33016 cell-line (DSM ACC 2219) grown in serum-free suspension culture was compared with the established MDCK CCL 34 cell-line (ATCC) and with chicken eggs, by determination of hemagglutinin (HA) titers, polymerase chain reaction (PCR), and virus titration.
  • HA hemagglutinin
  • PCR polymerase chain reaction
  • influenza positive samples were identified by diagnostic polymerase chain reaction (PCR). Susceptibility and reliability of influenza virus replication and isolation was assessed in the MDCK 33016 cell-line and in chicken eggs by: (i) hemagglutinin (HA) titers; (ii) real-time polymerase chain reaction (PCR) for viral load measurement; and (iii) virus titration. Replication accuracy in the cells was assessed by sequencing the HA gene in the original clinical specimens and also in isolates from the second passage in MDCK cells and the chicken eggs. Virus titers obtained from isolates grown in suspension MDCK 33016 cells were compared to those from MDCK 33016 cells adhered on plates.
  • PCR diagnostic polymerase chain reaction
  • amino acid substitutions were identified in no isolates.
  • nearly all egg-passaged viruses contained one or more amino acid substitutions, predominately in the HA1 gene. Mutations in the antibody binding site of the HA gene, observed following passage in eggs, may result in modifications to the antigenicity of the influenza virus.
  • the first harvest from each cell was further inoculated into eggs, for reference purposes.
  • the number of successful virus isolations, using each approach, is shown in the boxes in Figure 1 , with the number of different viral types injected also shown. All three virus subtypes isolated from the MDCK 33016 cells, gained reasonable HA (>32) and virus titer (>1x10 6 ) after the second passage in eggs, with both titers increasing with further passages ( Figure 2 ).
  • MDCK 33016 cells growing in suspension were superior to the adherent cell line (CCL-34) for isolation of influenza virus from clinical swabs for all three subtypes.
  • the suspension cell line showed a higher sensitivity for positive influenza swab material as demonstrated by the recovery rate (Table 1).
  • HA sequences were compared between different passages in MDCK 33016 cells and eggs to the original isolate (Table 2), and no mutations were found for influenza A strains isolated in MDCK 33016 cells even after 5 passages, whereas influenza A strains isolated in eggs showed mutations in the antibody binding site of the HA protein after 2 passages. No mutations were found for influenza B strains isolated in MDCK 33016 cells or eggs.
  • the MDCK 33016 suspension cell-line is an ideal system for the isolation and replication of wild-type influenza strains, as it offers a greater isolation capacity compared with chicken eggs. Furthermore, due to the high replication accuracy, the use of cell-based isolates for the production of a human influenza vaccine may lead to a more authentic vaccine. Improved match between the circulating wild-type strains and those contained in the vaccine should offer greater protection against influenza for the vaccinee.
  • the receptor preferences of original isolated viruses, of egg-grown viruses and of MDCK-grown viruses were investigated.
  • Figure 4 shows the results of a representative study.
  • the labelled peaks show binding to the SNA or MAA lectins.
  • the original virus (4A) and the virus grown on MDCK 33016 (4B) have distinct peaks for SNA & MAA, whereas the SNA & MAA peaks substantially overlap for egg-grown virus (4C).
  • Binding specificity was also examined in further experiments using 3-SL and 6-SLN. An example result is shown in Figure 5 . Binding on the left of the graph indicates an avian receptor preference, whereas binding on the right indicates a human receptor preference. As visible in Figure 5 , the cell-isolated virus strongly favours human receptors.
  • Figure 6 shows data using a Stuttgart isolate (A/H1N1) after 2 or 5 passages in eggs or in MDCK 33016 grown in suspension.
  • the MDCK-passaged viruses show a strong preference for 6-SLN.
  • Various strains of influenza A and B virus were isolated in MDCK cells and then passaged up to five times through one of the following substrates: eggs; MDCK cells CCL-34; MDCK cells 33016; Vero cells; or HEK 293-T cells.
  • the HA gene of the viruses were sequenced after each passage and HA titres were measured.
  • the HA sequence for some strains was stable during passage through eggs and through MDCK 33016, for others it was not.
  • the HA sequence of A/H1N1/Nordrhein Nonetheless/1/05 acquired a mutation D203N at antibody binding site D after 2 passages through eggs, and after 2 more passages it additionally acquired a R329K.
  • the sequence was unaltered in viruses passaged in parallel through MDCK 33016.

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