EP1711807A2 - Dispositif et procede pour coupler des procedes de separation capillaire a la spectrometrie de masse - Google Patents

Dispositif et procede pour coupler des procedes de separation capillaire a la spectrometrie de masse

Info

Publication number
EP1711807A2
EP1711807A2 EP05701173A EP05701173A EP1711807A2 EP 1711807 A2 EP1711807 A2 EP 1711807A2 EP 05701173 A EP05701173 A EP 05701173A EP 05701173 A EP05701173 A EP 05701173A EP 1711807 A2 EP1711807 A2 EP 1711807A2
Authority
EP
European Patent Office
Prior art keywords
capillary
capillaries
metal foil
monolithic
sorbent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP05701173A
Other languages
German (de)
English (en)
Inventor
Dieter Lubda
Ulrich Tallarek
Erdmann Rapp
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent GmbH filed Critical Merck Patent GmbH
Publication of EP1711807A2 publication Critical patent/EP1711807A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G01N30/724—Nebulising, aerosol formation or ionisation
    • G01N30/7266—Nebulising, aerosol formation or ionisation by electric field, e.g. electrospray
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0404—Capillaries used for transferring samples or ions
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H01J49/167—Capillaries and nozzles specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6052—Construction of the column body
    • G01N30/6073—Construction of the column body in open tubular form
    • G01N30/6078—Capillaries

Definitions

  • Figure 2 shows options without the use of carrier liquid, which are explained in more detail below: a) The electrical contact takes place via an OT-FS capillary, which is connected via a T-piece (8) between the separating capillary (5) and OT-FS ESI needle (11) is coupled and feeds the "make up" flow (10) there. b) The electrical contact is made via an electrode which is coupled via a T-piece (8) between the separating capillary (5) and the OT-FS-ESI needle (11). The connecting piece (9) itself can also serve as the electrode. c) The electrical contact is made directly via an open stainless steel capillary
  • Variants 2a to d react to changing conductivities of the mobile phase (e.g. with gradient elution).
  • the preferred direct sheathing of the separation capillary eliminates the need for an additional spray needle or an empty capillary. Dead volumes are avoided in this way. It has been found that covering the capillaries with metal foil is sufficient. A complex coating by spraying or sputtering is not necessary. The sheath is very durable and can be replaced at any time without great effort, without having to discard the entire separation capillary.
  • Figures 1 and 2 show different possibilities of an interface according to the state of the art.
  • Figure 3 shows three different embodiments of a capillary according to the invention. The dimensions and dimensions of the capillary columns can be found in Table 1. a) Partially packed capillary column. The electrical contact is made via a gold foil (22), which is directly on the spray end of the integrated ESI
  • FIG. 3b shows a capillary with monolithic sorbent (19), the end of which is cut off smoothly and does not taper to a point (20).
  • the gold foil (22) surrounds half of the end of the capillary and is slightly bent around the edge of the capillary (23) so that it lies directly against the opening of the capillary but does not protrude strongly into the cavity or channel of the capillary. So the geometry of the outlet opening is not affected. More detailed information on the dimensions of the capillary and the gold foil can be found in Table 1.
  • Voltages between 1600 and 2300 V are generally suitable for the Nano ESI mode. Voltages between 2800-5500 V are generally suitable for normal ESI mode.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

L'invention concerne des capillaires, qui sont au moins partiellement enrobés d'un film métallique, ainsi que leur utilisation pour coupler des procédés, tels que la CLHP, l'EC (électrophorèse capillaire), l'ECC (électrochromatographie capillaire) ou l'ECCp (ECC sous pression), à la SM (spectrométrie de masse). L'enrobage au moyen d'un film métallique selon l'invention permet de coupler directement les capillaires à un spectromètre de masse sans avoir recours à d'autres raccords, tels que des aiguilles de nébulisation ou des parties de capillaires vides.
EP05701173A 2004-02-05 2005-01-25 Dispositif et procede pour coupler des procedes de separation capillaire a la spectrometrie de masse Withdrawn EP1711807A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004005888A DE102004005888A1 (de) 2004-02-05 2004-02-05 Vorrichtung und Verfahren zur Kopplung von kapillaren Trennverfahren und Massenspektrometrie
PCT/EP2005/000712 WO2005075976A2 (fr) 2004-02-05 2005-01-25 Dispositif et procede pour coupler des procedes de separation capillaire a la spectrometrie de masse

Publications (1)

Publication Number Publication Date
EP1711807A2 true EP1711807A2 (fr) 2006-10-18

Family

ID=34801672

Family Applications (1)

Application Number Title Priority Date Filing Date
EP05701173A Withdrawn EP1711807A2 (fr) 2004-02-05 2005-01-25 Dispositif et procede pour coupler des procedes de separation capillaire a la spectrometrie de masse

Country Status (5)

Country Link
US (1) US20080315083A1 (fr)
EP (1) EP1711807A2 (fr)
JP (1) JP2007520711A (fr)
DE (1) DE102004005888A1 (fr)
WO (1) WO2005075976A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101617224B (zh) * 2007-01-12 2014-03-26 得克萨斯州大学系统董事会 连接低流速分离技术
WO2008087715A1 (fr) 2007-01-17 2008-07-24 Shimadzu Corporation Emetteur d'ionisation, appareil d'ionisation et procede de production d'emetteur d'ionisation
US8895918B2 (en) * 2011-06-03 2014-11-25 Purdue Research Foundation Ion generation using modified wetted porous materials
CN102522311A (zh) * 2011-10-20 2012-06-27 中国科学院上海有机化学研究所 一种纳喷雾电极及其制作方法和应用
US9196468B2 (en) * 2012-05-18 2015-11-24 Dh Technologies Development Pte. Ltd. Method and system for introducing make-up flow in an electrospray ion source system
BE1021813B1 (fr) * 2014-02-13 2016-01-19 Analis Sa Interface ce-ms
US9786478B2 (en) 2014-12-05 2017-10-10 Purdue Research Foundation Zero voltage mass spectrometry probes and systems
JP6948266B2 (ja) 2015-02-06 2021-10-13 パーデュー・リサーチ・ファウンデーションＰｕｒｄｕｅ Ｒｅｓｅａｒｃｈ Ｆｏｕｎｄａｔｉｏｎ プローブ、システム、カートリッジ、およびその使用方法
US20190019662A1 (en) * 2017-07-14 2019-01-17 Purdue Research Foundation Electrophoretic mass spectrometry probes and systems and uses thereof
CN107702949A (zh) * 2017-08-15 2018-02-16 杭州臻盛科技有限公司 顶空取样针以及取样方法
WO2019053850A1 (fr) * 2017-09-14 2019-03-21 株式会社島津製作所 Chromatographe en phase liquide
WO2019053851A1 (fr) * 2017-09-14 2019-03-21 株式会社島津製作所 Pulvérisateur esi et dispositif d'ionisation
DE102018000650A1 (de) 2018-01-27 2019-08-01 Friedrich-Schiller-Universität Jena Verfahren zur Bestimmung von Verunreinigungen in Polyalkylenethern oder Polyalkylenaminen und dessen Verwendung
DE102018103609B4 (de) 2018-02-19 2021-06-02 Bruker Daltonik Gmbh Flüssigkeitsanschluss-Einrichtung für die Ionisierung durch Elektrosprühen
CN113075114B (zh) * 2019-12-17 2022-07-01 北京大学 一种用于单细胞分析的有机质谱流式分析方法
GB202211210D0 (en) * 2022-08-01 2022-09-14 Micromass Ltd Electrospray device

Citations (3)

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Publication number Priority date Publication date Assignee Title
DE10028447A1 (de) * 2000-06-14 2001-12-20 Merck Patent Gmbh Verfahren zur Herstellung von monolithischen Chromatographiesäulen
WO2002066135A1 (fr) * 2001-02-20 2002-08-29 Advion Biosciences, Inc. Dispositif d'electronebulisation a micropuce et colonne comprenant des adsorbents d'affinite et utilisation
US6525313B1 (en) * 2000-08-16 2003-02-25 Brucker Daltonics Inc. Method and apparatus for an electrospray needle for use in mass spectrometry

Family Cites Families (9)

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Publication number Priority date Publication date Assignee Title
US3926271A (en) * 1975-02-03 1975-12-16 Rupprecht Georg Microbalance
IL64041A0 (en) * 1980-11-03 1982-01-31 Hughes Aircraft Co Metallic clad glass capillary tubing
JPS5922112U (ja) * 1982-08-03 1984-02-10 丹野　直弘 芯管入同軸型ガラス毛細管電極
US4726822A (en) * 1984-10-22 1988-02-23 Honeywell Inc. Fast response thermochromatographic capillary columns
US6297499B1 (en) * 1997-07-17 2001-10-02 John B Fenn Method and apparatus for electrospray ionization
JP2001074697A (ja) * 1999-09-07 2001-03-23 Jeol Ltd エレクトロスプレー・イオン源
AU1189702A (en) * 2000-10-13 2002-04-22 Fluidigm Corp Microfluidic device based sample injection system for analytical devices
JP2003331776A (ja) * 2002-05-10 2003-11-21 Hitachi Ltd イオン源および質量分析装置および質量分析方法
JP4613002B2 (ja) * 2003-10-29 2011-01-12 株式会社日立ハイテクノロジーズ エレクトロスプレイ用カラム一体型チップの製造方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10028447A1 (de) * 2000-06-14 2001-12-20 Merck Patent Gmbh Verfahren zur Herstellung von monolithischen Chromatographiesäulen
US6525313B1 (en) * 2000-08-16 2003-02-25 Brucker Daltonics Inc. Method and apparatus for an electrospray needle for use in mass spectrometry
WO2002066135A1 (fr) * 2001-02-20 2002-08-29 Advion Biosciences, Inc. Dispositif d'electronebulisation a micropuce et colonne comprenant des adsorbents d'affinite et utilisation

Also Published As

Publication number Publication date
DE102004005888A1 (de) 2005-08-25
WO2005075976A2 (fr) 2005-08-18
WO2005075976A3 (fr) 2005-12-15
JP2007520711A (ja) 2007-07-26
US20080315083A1 (en) 2008-12-25

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