EP1221445B1 - Pentacyclische taxan-verbindungen - Google Patents

Pentacyclische taxan-verbindungen Download PDF

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EP1221445B1
EP1221445B1 EP00966445A EP00966445A EP1221445B1 EP 1221445 B1 EP1221445 B1 EP 1221445B1 EP 00966445 A EP00966445 A EP 00966445A EP 00966445 A EP00966445 A EP 00966445A EP 1221445 B1 EP1221445 B1 EP 1221445B1
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group
compound
salt
following formula
pyridyl
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EP1221445A4 (de
EP1221445A1 (de
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Tsunehiko Daiichi Sankyo Co. Ltd.Kasai R&D Center SOGA
Kouichi Daiichi Sankyo Co. Ltd.Kasai R&D Center UOTO
Yasuyuki Daiichi Sankyo Co. Ltd.Kasai R&D Center TAKEDA
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Daiichi Sankyo Co Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
    • C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
    • C07D493/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/4353—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems
    • A61K31/4355—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a five-membered ring having oxygen as a ring hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

Definitions

  • This invention relates to a taxol derivative which can be administered orally and has an antitumor activity.
  • Taxol is a natural substance represented by the following chemical structural formula, which can be obtained in a small amount from a bark or other parts of Taxus brevifolia.
  • taxol has an antitumor activity, and its action mechanism is considered to be based on its action to inhibit depolymerization of microtubules in cell division, so that its clinical application is expected as an antitumor agent having an action mechanism which is different from the conventional antitumor agents.
  • Taxol has so far been obtained from a natural source but only in an extremely small amount.
  • taxol derivatives synthesized using a taxol precursor 10-O-deacetylbaccatine III represented by the following formula, which can be obtained from leaves and other parts of Taxus plants in a relatively large amount have been reported.
  • compound A a compound having a structure of the following formula has been drawing attention as a compound having an antitumor activity equal to or higher than that of taxol, and its development as an antitumor agent is in progress.
  • JP-A-9-12578 the term "JP-A” as used herein refers to a "published unexamined Japanese patent application”).
  • Taxol, taxotere and the compound disclosed in JP-A-9-12578 are promising as antitumor agents. Regarding the compounds disclosed in Examples of JP-A-9-12578 , however, it has a drawback from the toxicity point of view, the efficacy of these compounds by oral administration is not known. From the viewpoint, for example, of lightening the burden on patients at the time of administration and of medical economy, a taxol derivative which can be orally administered is in demand.
  • compound B a compound of the following formula capable of showing a significant antitumor activity even by its oral administration for example in an antitumor test using mice.
  • the invention provides a compound represented by the following formula or a salt thereof, a medicament which comprises the compound of the following formula or a salt thereof and an antitumor agent which contains the compound of the following formula or a salt thereof.
  • the invention also provides an intermediate (hereinafter, referred to as "intermediate of the invention") represented by the following formula for use in the production of the taxol derivative, and use thereof.
  • R 1 is dimethylaminomethyl group or morpholinomethyl group and R 2 is a halogen atom or an alkoxy group having from 1 to 6 carbon atoms.
  • R 2 include methoxy group, fluorine atom and chlorine atom, more preferably fluorine atom and methoxy group.
  • Particularly preferred is a compound represented by the following formula, namely (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-acetoxy-2-benzoyloxy-9,10-[(1S)-2-(dimethylamino)ethylidenedioxy]-5,20-epoxy-1-hydroxytax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-hydroxypropionate, or a salt thereof.
  • R 3 is dimethylaminomethyl group, morpholinomethyl group or vinyl group
  • R 4 is hydroxyl group which may have a protecting group
  • R 5 is an alkoxy group having from 1 to 6 carbon atoms or a halogen atom.
  • the part of dotted line between the 6-position and 7-position of a partial structure in the intermediate of the invention, shown by the following formula means that the bond of the part may be a double bond.
  • examples of the protecting group of R 4 include a trialkylsilyl group, benzyl group, a substituted benzyl group, 1-ethoxyethyl group, benzyloxycarbonyl group and 2,2,2-trichloroethoxycarbonyl group.
  • a trialkylsilyl group such as triisopropylsilyl group, tertiary butyldimethylsilyl group or triethylsilyl group and benzyl group, and particularly preferred are triisopropylsilyl group and benzyl group.
  • the production intermediate of the taxol derivative of the invention can be used by optionally selecting it in response to the final product of interest.
  • a compound of the following formula or a salt thereof it is desirable to use a compound of the following formula (wherein R 6 is triisopropylsilyl group, tertiary butyldimethylsilyl group, triethylsilyl group or benzyl group) or a salt thereof, a compound of the following formula (wherein R 7 is triisopropylsilyl group, tertiary butyldimethylsilyl group, triethylsilyl group or benzyl group) or a salt thereof, or a compound of the following formula (wherein R 8 is triisopropylsilyl group, tertiary butyldimethylsilyl group, triethylsilyl group or benzyl group) or a salt thereof.
  • the compound of the invention may be in its free form or an acid addition salt.
  • the acid addition salt include inorganic acid salts such as hydrochloride, sulfate, nitrate, hydrobromide, hydroiodide and phosphate or organic acid salts such as acetate, methane sulfonate, benzenesulfonate, toluenesulfonate, citrate, maleate, fumarate and lactate.
  • solvent examples include water, methanol, ethanol, propanol, butanol, acetone, acetonitrile, benzene, toluene, tetrahydrofuran and N,N-dimethylformamide.
  • the compound of the invention can be synthesized in accordance with the method reported in JP-A-9-12578 , for example, the following synthetic methods.
  • the reaction may be carried out by protecting substituent groups with protecting groups as occasion demands, but the operating order of their deprotection is not particularly limited.
  • a compound (3) is obtained by condensing a compound (1) with a compound (2) in the presence of a base. Then, a protecting group on the hydroxyl group of the thus obtained compound (3) is removed to give a compound (4).
  • the terminal olefin thereof is converted into a diol using an oxidizing agent such as N-methylmorpholine-N-oxide in the presence of an osmium tetraoxide catalyst and then cleaved oxidatively using sodium periodate and the like to form an aldehyde. Thereafter, a reductive reaction with the corresponding amine is carried out to obtain a compound (5).
  • a compound (7) is obtained by condensing a compound (6) with a compound (2) in the same manner as in Synthetic Method 1. Then a compound (8) can be obtained by the conversion of the terminal olefin thereof in the same manner as in Synthetic Method 1. Thereafter, a compound (9) is obtained by reducing the olefin at the 6 and 7-positions by the hydrogenation, and then a protecting group on the hydroxyl group is finally removed, thereby obtaining a compound (5).
  • the starting materials (1) and (6) in the above Synthetic Methods 1 and 2 can be synthesized in accordance with the method reported in JP-A-9-12578 .
  • the compound (2) can be synthesized in accordance with a known synthetic method for ⁇ -lactam compound reported by a literature (for example, see J. Am. Chem. Soc. 110, 1917 (1988 )).
  • R 1 , R 2 and R 6 are as defined in the foregoing.
  • Boc means tertiary butoxycarbonyl group
  • Ac means acetyl group
  • the medicament of the invention can realize treatment of cancers based on its antitumor action, and examples of the object to be treated include various cancers such as lung cancer, gastrointestinal cancer, ovarian cancer, uterine cancer, breast cancer, cancer of liver, cancer of head and neck, blood cancer, renal cancer and testicular tumor.
  • various cancers such as lung cancer, gastrointestinal cancer, ovarian cancer, uterine cancer, breast cancer, cancer of liver, cancer of head and neck, blood cancer, renal cancer and testicular tumor.
  • the compound of the invention can be administered as various injections such as for intravenous injection, intramuscular injection and subcutaneous injection, or by various methods such as oral administration and percutaneous administration.
  • oral administration is desirable from the viewpoint of achieving the effects which will be described later.
  • oral administration it may be any of free compound or salts.
  • the compound of the invention When a test was carried out using cancer-free mice, the compound of the invention showed no renal toxicity.
  • Applicability of the compound of the invention as an oral preparation can be predicted by an in vitro test which uses human liver microsome.
  • the drug dissolves in the gastrointestinal tract, undergoes its metabolism in the digestive tract and liver and then enters into the blood circulation system. Accordingly, it is considered that metabolism of the drug in the liver exerts influence on the expression of efficacy of the drug.
  • the compound of the invention and its analogous compounds undergo their metabolism by CYP3A which is an enzyme distributed in the liver microsome.
  • prediction of the metabolism by an in vitro test using liver microsome is important in considering its clinical use in practice. It has been reported, for example in Pharm. Tech. Japan, 13, 17 - 39, 1997 and J. Pharmacol. Exp.
  • liver microsome is available for example from Xenotech LLC, and measurement of the metabolic rate can be carried out making reference to the above journals.
  • bioavailability of the drug can also be calculated as a theoretical value ( J. Pharmacol. Exp. Ther., 283, 46 - 58, 1997 ). Bioavailability is defined as the amount and rate of a drug which reaches the systemic circulating blood, relative to the administered drug ( Pharmacokinetic Studies on Drug Development, edited by Yuichi Sugiyama, p. 15, published by Yakuji Jiho ).
  • bioavailability is defined as the amount and rate of a drug which reaches the systemic circulating blood, relative to the administered drug ( Pharmacokinetic Studies on Drug Development, edited by Yuichi Sugiyama, p. 15, published by Yakuji Jiho ).
  • there are various obstacles until a drug enters into the circulating blood such as dissolution in the gastrointestinal tract, passage through the digestive tract mucous membrane and metabolism in the digestive tract and liver.
  • antitumor agents In the case of antitumor agents, they are administered mostly at around the maximum tolerated dose in order to increase response rate, so that the therapeutic range and the toxicity range draw close to each other and the safety range becomes narrow as the result. Thus, it becomes difficult to use a drug having a large variability range of individual bioavailability as an antitumor agent.
  • the compound of the invention According to the compound of the invention, its metabolic rate in human liver microsome was reduced, and the theoretical value of bioavailability of its unchanged form was also improved. Thus, it was predicted that the variability range of bioavailability values of the unchanged compound among individuals would be small. Because of this effect, it is sufficiently possible to carry out oral administration of the compound of the invention from the safety point of view in enlarging safety range and from viewpoint of effective drug efficacy expression.
  • theoretical bioavailability value of the unchanged compound is preferably 0.4 or more, more preferably 0.7 or more.
  • applicability of the compound of the invention as oral preparations can also be predicted by a bioavailability (BA) test using monkeys. Metabolism of the compound (B) by the liver microsome of mouse and dog is slow, and its oral absorption property is actually excellent. On the other hand, its metabolism by the monkey liver microsome is quick similar to the case of the human liver microsome. In this case, oral absorption property of the compound (B) in monkey is low. On the contrary, metabolism of the compound of the invention by the monkey liver microsome is slow similar to the case of the mouse and dog liver microsome.
  • bioavailability (BA) was measured using monkeys for the purpose of confirming the oral absorption improving effect by the inhibition of metabolism, it was confirmed that the oral absorption in monkey was sharply improved by the compound of the invention in comparison with the compound (B).
  • the method for the preparation of pharmaceutical preparations of medicaments and antitumor agents can be prepared by selecting appropriate pharmaceutical preparation in response to its administration method and employing a usually used preparation method.
  • dosage forms of the antitumor agent of the invention tablets, powders, granules and capsules can be exemplified as the preparations for oral administration use.
  • examples of other dosage forms include solutions, syrups, elixirs and oily or aqueous suspensions.
  • capsules, tablets and solutions are desirable.
  • additives such as a stabilizer, an antiseptic and a solubilization assisting agent can be used in the preparation.
  • a solution which may contain such auxiliary substances is made into a solid preparation by freeze-drying or the like means, it can be used as a pharmaceutical preparation which is dissolved before use.
  • Solutions, suspensions and emulsions can be exemplified as the liquid preparation, and additive agents such as a suspending agent and an emulsifying agent can be used when these pharmaceutical preparations are prepared.
  • the compound of the invention can be used for the treatment of cancers in mammals, particularly in human, and when administered to human, it is desirable to administer it once a day and repeat it at appropriate intervals.
  • Boc means tertiary butoxycarbonyl group
  • Ac means acetyl group
  • Bz means benzoyl group
  • TIPS means triisopropylsilyl group.
  • Step 1 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-methoxy-2-pyridyl)-2-triisopropylsilyloxypropionate
  • Step 2 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-2-hydroxy-3-(5-methoxy-2-pyridyl)propionate
  • a 530 mg portion of the compound obtained in the above step 1 was dissolved in 10 ml of dry tetrahydrofuran, 1.0 ml of tetrabutylammonium fluoride (1M tetrahydrofuran solution) was added to the solution under ice-cooling and then the mixture was stirred at the same temperature for 30 minutes. Water and ethyl acetate were added to the reaction solution to carry out separation of layers, and the water layer was extracted with ethyl acetate. The organic layers were combined, washed with saturated sodium bicarbonate aqueous solution and saturated brine in that order and then dried using anhydrous sodium sulfate.
  • Step 3 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-[(1S)-2-(morpholino)ethylidenedioxy]tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-2-hydroxy-3-(5-methoxy-2-pyridyl)propionate
  • a 400 mg portion of the compound obtained in the above step 2 was dissolved in 5 ml of tetrahydrofuran, and the solution was mixed with 5 ml of acetone, 5 ml of water, 5.9 mg of osmium tetroxide and 270 mg of N-methylmorpholine-N-oxide and stirred at room temperature for 4.5 hours.
  • Ethyl acetate and 10% sodium thiosulfate aqueous solution were added to the reaction solution to carry out separation of layers, and the water layer was extracted with ethyl acetate.
  • the organic layers were combined, washed with saturated sodium bicarbonate aqueous solution and saturated brine in that order and then dried with anhydrous sodium sulfate.
  • Step 1 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-chloro-2-pyridyl)-2-triisopropylsilyloxypropionate
  • the title compound was obtained by repeating the same procedure of the step 1 of Example 1, except that (3R,4S)-1-(tert-butoxycarbonyl)-4-(5-chloro-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone was used instead of (3R,4S)-1-(tert-butoxycarbonyl)-4-(5-methoxy-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone.
  • Step 2 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-chloro-2-pyridyl)-2-hydroxypropionate
  • Step 3 (1S,2S,3R,4S,SR,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-[(1S)-2-(morpholino)ethylidenedioxy]tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-chloro-2-pyridyl)-2-hydroxypropionate
  • Step 1 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-fluoro-2-pyridyl)-2-triisopropylsilyloxypropionate
  • the title compound was obtained by carrying out the same procedure of the step 1 of Example 1, except that (3R,4S)-1-(tert-butoxycarbonyl)-4-(5-fluoro-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone was used instead of (3R,4S)-1-(tert-butoxycarbonyl)-4-(5-methoxy-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone.
  • Step 2 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-fluoro-2-pyridyl)-2-hydroxypropionate
  • Step 3 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-[(1S)-2-(morpholino)ethylidenedioxy]tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-fluoro-2-pyridyl)-2-hydroxypropionate
  • Step 1 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-triisopropylsilyloxypropionate
  • the title compound was obtained by carrying out the same procedure of the step 1 of Example 1, except that (3R,4S)-1-(tert-butoxycarbonyl)-4-(3-fluoro-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone was used instead of (3R,4S)-1-(tert-butoxycarbonyl)-4-(5-methoxy-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone.
  • Step 2 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-hydroxypropionate
  • Step 3 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-[(1S)-2-(morpholino)ethylidenedioxy]tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-hydroxypropionate
  • Step 1 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-6,11-dien-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-methoxy-2-pyridyl)-2-triisopropylsilyloxypropionate
  • Step 2 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-[(1S)-2-(morpholino)ethylidenedioxy]tax-6,11-dien-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-methoxy-2-pyridyl)-2-triisopropylsilyloxy propionate
  • a 520 mg portion of the compound obtained in the above step 1 was dissolved in 5 ml of tetrahydrofuran, and the solution was mixed with 5 ml of acetone, 5 ml of water, 13 mg of osmium tetraoxide and 300 mg of N-methylmorpholine-N-oxide and stirred at room temperature for 7.5 hours.
  • Ethyl acetate and 10% sodium thiosulfate aqueous solution were added to the reaction solution to carry out separation of layers, and the water layer was extracted with ethyl acetate.
  • the organic layers were combined, washed with saturated sodium bicarbonate aqueous solution and saturated brine in that order and then dried with anhydrous sodium sulfate.
  • Step 3 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-[(1S)-2-(morpholino)ethylidenedioxy]tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(5-methoxy-2-pyridyl)-2-triisopropylsilyloxypropionate
  • a 235 mg portion of the compound obtained in the above step 2 was dissolved in 10 ml of ethanol, and the solution was mixed with 235 mg of 5% palladium-carbon catalyst (wet) and shaken for 10 hours under a hydrogen pressure (392 kPa). After removing the catalyst by filtration, the filtrate was concentrated to obtain 230 mg of the title compound.
  • Step 4 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-[(1S)-2-(morpholino)ethylidenedioxy]tax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-2-hydroxy-3-(5-methoxy-2-pyridyl)propionate
  • Step 1 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-6,11-dien-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-triisopropylsilyloxypropionate
  • the title compound was obtained by carrying out the same procedure of the step 1 of Example 8, except that (3R,4S)-1-(tert-butoxycarbonyl)-4-(3-fluoro-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone was used instead of (3R,4S)-1-(tert-butoxycarbonyl)-4-(5-methoxy-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone.
  • Step 2 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-9,10-[(1S)-2-(dimethylamino)ethylidenedioxy]-5,20-epoxy-1-hydroxytax-6,11-dien-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-triisopropylsilyloxypropionate
  • Step 3 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-9,10-[(1S)-2-(dimethylamino)ethylidenedioxy]-5,20-epoxy-1-hydroxytax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-triisopropylsilyloxypropionate
  • Step 4 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-9,10-[(1S)-2-(dimethylamino)ethylidenedioxy]-5,20-epoxy-1-hydroxytax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-hydroxypropionate
  • the title compound was obtained by carrying out the same procedure of the step 4 of Example 8, except that the compound obtained in the above step 3 was used as the material. Its instrumental analysis data coincided with those of the compound obtained in the step 3 of Example 7.
  • Step 1 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-5,20-epoxy-1-hydroxy-9,10-(2-propenylidenedioxy)tax-6,11-dien-13-yl (2R,3S)-2-benzyloxy-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)propionate
  • the title compound was obtained by carrying out the same procedure of the step 1 of Example 8, except that (3R,4S)-3-benzyloxy-1-(tert-butoxycarbonyl)-4-(3-fluoro-2-pyridyl)-2-azetidinone was used instead of (3R,4S)-1-(tert-butoxycarbonyl)-4-(5-methoxy-2-pyridyl)-3-triisopropylsilyloxy-2-azetidinone.
  • Step 2 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-9,10-[(1S)-2-(dimethylamino)ethylidenedioxy]-5,20-epoxy-1-hydroxytax-6,11-dien-13-yl (2R,3S)-2-benzyloxy-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)propionate
  • Step 3 (1S,2S,3R,4S,5R,8R,9S,10R,13S)-4-Acetoxy-2-benzoyloxy-9,10-[(1S)-2-(dimethylamino)ethylidenedioxy]-5,20-epoxy-1-hydroxytax-11-en-13-yl (2R,3S)-3-(tert-butoxycarbonylamino)-3-(3-fluoro-2-pyridyl)-2-hydroxypropionate
  • the title compound was obtained by carrying out the same procedure of the step 3 of Example 8, except that the compound obtained in the above step 2 was used as the material. Its instrumental analysis data coincided with those of the compound obtained in the step 3 of Example 7.
  • mice Mouse fibrosarcoma Meth A was subcutaneously transplanted into mice (line name; Balb/c), and each compound dissolved in a mixed solvent of ethanol, Tween 80 and 5% glucose (5:5:90 (v/v)) was administered by intravenous injection after 6, 8 or 10 days (or only after 6 days) of the transplantation. Each animal was anatomized on the 17th day to examine tumor weight, platelet numbers and renal toxicity. Six mice were used in each group.
  • Antitumor effect was calculated by the following formula. 1 - tumor weight of compound - administered group / tumor weight of solvent - administered group ⁇ 100
  • the number of platelets was expressed by (platelets of compound-administered group/platelets of solvent-administered group) x 100.
  • the renal toxicity findings were expressed as "changed" when a change such as fading was found by macroscopic observation at the time of anatomy or when a change such as precipitation of hyaline droplet substance in the renal tubular cell cytoplasm was found by a histological inspection.
  • B16 Melanoma BL6 was subcutaneously transplanted into mice (C57BL/6), and each compound was administered 4 days thereafter.
  • the compound of formula A was administered by dissolving it in a mixed solvent of ethanol, Tween 80 and 5% glucose (5:15:80 (v/v)), and the compound of Example 7 by dissolving in the same mixed solvent of 5:5:90 (v/v).
  • each compound was administered by suspending it in a 0.5% carboxymethylcellulose sodium aqueous solution. Body weight was measured every 2 or 3 days after the administration, and each animal was anatomized after 15 days of the transplantation to measure tumor weight. Antitumor effect was calculated by the following formula. 1 - tumor weight of compound - administered group / tumor weight of solvent - administered group ⁇ 100
  • Each of the samples to be evaluated was dissolved in acetonitrile/water (1:1, v/v) to a concentration of 500 ⁇ M, and the solution was mixed with human liver microsome (Xenotech LLC) and other components such as various coenzymes and buffer solution and allowed to generate the metabolic reaction at 37°C.
  • human liver microsome Xenotech LLC
  • the reaction solution was composed of phosphate buffer (0.076 M; final concentration, the same shall apply hereinafter), sample to be evaluated (10 ⁇ M), human liver microsome (1 mg/ml), glucose 6-phosphate (10 mM), glucose-6-phosphate dehydrogenase (1 unit/ml), magnesium chloride (4 mM) and reduced nicotinamide adenine dinucleotide phosphate ( ⁇ -NADPH, 1 mM), and 500 ⁇ l of the solution was used in one reaction.
  • the reaction solution excluding ⁇ -NADPH was incubated in advance at 37°C for 2 minutes and then the reaction was started by adding a ⁇ -NADPH aqueous solution (50 mM, 10 ⁇ l).
  • the reaction was terminated by adding 1 ml of ice-cooled acetonitrile 1, 2 or 5 minutes after commencement of the reaction.
  • a sample of 0 minute after commencement of the reaction was prepared by adding water instead of the ⁇ -NADPH aqueous solution and immediately adding 1 ml of acetonitrile.
  • a 100 ⁇ l portion of an internal standard substance was added to each of these samples, and the reaction solution was centrifuged for 15 minutes. The resulting supernatant was injected into a high performance liquid chromatography (HPLC) to measure concentration of the sample to be evaluated. The amount decreased from the concentration at 0 minute of the commencement of reaction was used as the formed amount of the metabolite (nmol/mg protein).
  • the liver-specific clearance (CLint) was calculated by the following formula.
  • CLint ml / min / kg body weight k ⁇ ( g liver weight ) / kg body weight ⁇ ( 45 mg of microsome protein ) / g liver weight wherein the liver weight per 1 kg body weight is 20 g.
  • the compound of formula (B) or of Example 7 was intravenously or orally administered to a monkey by single dose, and changes in its blood concentration was measured to calculate AUC 0- ⁇ .
  • the AUC 0- ⁇ means area under the blood concentration time curve of a drug concentration in blood during a period of from the time of administration (0 h) to the infinite time and it can be calculated in accordance with the method (trapezoidal rule) disclosed in Kiyoshi Yamaoka and Yusuke Tanigawara, Yakubutsu Sokudoron Nyumon (A Guide to Pharmacokinetics), pages 116 - 117 .
  • ratio of the AUC at the time of oral administration to the AUC at the time of intravenous administration was calculated as oral BA.
  • Test of the compound of formula (B) was carried out using different individual of one monkey for the intravenous and oral administration, and test of the compound of Example 7 was carried out using the same individuals of 4 monkeys for the intravenous and oral administration to calculate average AUC value.
  • the compound of the invention was improved in terms of its toxicity and showed no renal toxicity.
  • the compound of the invention showed high antitumor effect by its oral administration to mice. Since the compound of the invention has a large theoretical F value of its unchanged form, the variability range of bioavailability is suppressed and separation of the therapeutic range and toxicity range can be effected.
  • the compound of the invention showed excellent oral absorption property in monkey. Accordingly, the compound of the invention can be used as an antitumor agent which can be orally administered.

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Claims (13)

  1. Verbindung der folgenden Formel
    Figure imgb0049
     (worin R1 für eine Dimethylaminomethylgruppe oder Morpholinomethylgruppe steht und R2 für ein Halogenatom oder eine Alkoxygruppe mit 1 bis 6 Kohlenstoffatomen steht) oder ein Salz davon.
  2. Verbindung oder ein Salz davon nach Anspruch 1, wobei R2 für eine Methoxygruppe oder ein Fluoratom steht.
  3. Verbindung nach Anspruch 1 der folgenden Formel
    Figure imgb0050
     oder ein Salz davon.
  4. Medikament, das eine in einem der Ansprüche 1 bis 3 beschriebene Verbindung oder ein Salz davon umfasst.
  5. Antitumormittel, das eine in einem der Ansprüche 1 bis 3 beschriebene Verbindung oder ein Salz davon enthält.
  6. Verbindung der folgenden Formel
    Figure imgb0051
     (worin R3 für eine Dimethylaminomethylgruppe, Morpholinomethylgruppe oder Vinylgruppe steht, R4 für eine Hydroxylgruppe, die eine Schutzgruppe aufweisen kann, steht und R5 für ein Halogenatom oder eine Alkoxygruppe mit 1 bis 6 Kohlenstoffatomen steht und der Teil der gestrichelten Linie zwischen der 6-Position und 7-Position der Teilstruktur der Formel
    Figure imgb0052
     bedeutet, dass die Bindung des erwähnten Teils eine Doppelbindung sein kann (oder ein Salz davon).
  7. Verbindung nach Anspruch 6 der folgenden Formel
    Figure imgb0053
     (worin R6 eine Triisopropylsilylgruppe, tertiäre-Butyldimethylsilylgruppe, Triethylsilylgruppe oder Benzylgruppe bedeutet) oder ein Salz davon.
  8. Verbindung nach Anspruch 6 der folgenden Formel
    Figure imgb0054
     (worin R7 eine Triisopropylsilylgruppe, tertiäre-Butyldimethylsilylgruppe, Triethylsilylgruppe oder Benzylgruppe bedeutet) oder ein Salz davon.
  9. Verbindung nach Anspruch 6 der folgenden Formel:
    Figure imgb0055
     (worin R8 eine Triisopropylsilylgruppe, tertiäre-Butyldimethylsilylgruppe, Triethylsilylgruppe oder Benzylgruppe bedeutet) oder ein Salz davon.
  10. Verwendung einer in einem der Ansprüche 6 bis 9 beschriebenen Verbindung oder eines Salzes davon zur Herstellung einer in einem der Ansprüche 1 bis 3 beschriebenen Verbindung oder eines Salzes davon.
  11. Verwendung nach Anspruch 10 einer Verbindung der folgenden Formel
    Figure imgb0056
     (worin R6 eine Triisopropylsilylgruppe, tertiäre-Butyldimethylsilylgruppe, Triethylsilylgruppe oder Benzylgruppe bedeutet) oder eines Salzes davon, zur Herstellung einer Verbindung der folgenden Formel
    Figure imgb0057
     oder eines Salzes davon.
  12. Verwendung nach Anspruch 10 einer Verbindung der folgenden Formel
    Figure imgb0058
     (worin R7 eine Triisopropylsilylgruppe, tertiäre-Butyldimethylsilylgruppe, Triethylsilylgruppe oder Benzylgruppe bedeutet) oder eines Salzes davon, zur Herstellung einer Verbindung der folgenden Formel
    Figure imgb0059
     oder eines Salzes davon.
  13. Verwendung nach Anspruch 10 einer Verbindung der folgenden Formel
    Figure imgb0060
     (worin R8 eine Triisopropylsilylgruppe, tertiäre-Butyldimethylsilylgruppe, Triethylsilylgruppe oder Benzylgruppe bedeutet) oder eines Salzes davon, zur Herstellung einer Verbindung der folgenden Formel
    Figure imgb0061
     oder eines Salzes davon.
EP00966445A 1999-10-15 2000-10-12 Pentacyclische taxan-verbindungen Expired - Lifetime EP1221445B1 (de)

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JP29335699 1999-10-15
JP29335699 1999-10-15
PCT/JP2000/007087 WO2001027115A1 (fr) 1999-10-15 2000-10-12 Composes pentacycliques au taxane

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TW200533339A (en) 2004-03-16 2005-10-16 Bristol Myers Squibb Co Therapeutic synergy of anti-cancer compounds
ES2371947T3 (es) * 2004-04-30 2012-01-11 Daiichi Sankyo Company, Limited Proceso para producir taxano pentacíclico.
JPWO2007049575A1 (ja) * 2005-10-25 2009-04-30 第一三共株式会社 アゼチジン環構造を有するタキサン化合物
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US8785669B2 (en) * 2010-06-30 2014-07-22 Gfv, Llc Taxane compounds, compositions and methods
TW201316990A (zh) 2011-08-02 2013-05-01 Astellas Pharma Inc 藥劑併用之癌治療方法
FR2986526A1 (fr) 2012-02-03 2013-08-09 Sanofi Sa Procede de preparation de derives du taxol et certains composes intermediaires
CN104650109B (zh) * 2013-11-22 2019-01-01 江苏天士力帝益药业有限公司 紫杉烷类化合物

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IL149007A0 (en) 2002-11-10
PT1221445E (pt) 2008-10-08
MXPA02003797A (es) 2002-12-13
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CA2386772A1 (en) 2001-04-19
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