EP1051623A1 - Troponin-bestimmung ohne heparin-störung - Google Patents
Troponin-bestimmung ohne heparin-störungInfo
- Publication number
- EP1051623A1 EP1051623A1 EP99901673A EP99901673A EP1051623A1 EP 1051623 A1 EP1051623 A1 EP 1051623A1 EP 99901673 A EP99901673 A EP 99901673A EP 99901673 A EP99901673 A EP 99901673A EP 1051623 A1 EP1051623 A1 EP 1051623A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- heparin
- troponin
- tnl
- polybrene
- cardiac
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6887—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids from muscle, cartilage or connective tissue
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
- G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
- G01N2333/4701—Details
- G01N2333/4712—Muscle proteins, e.g. myosin, actin, protein
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Definitions
- troponin is a myofib llar protein complex, made up of three proteins, troponins I, T and C. This protein complex helps to regulate muscle contraction by the Ca 2+ ion, interacting with myosin and actin. More precisely, it is known that when a nerve impulse arrives at the level of the motor plaque of a muscle, there is generation of an action potential which is transmitted to the sarcoplasmic reticulum. Ca 2+ is then released into the cytosol and binds to troponin C, which leads to a strengthening of the interaction between troponin I and troponin C and, consequently, a change in conformation of the troponin I, T complex. , C. There is then release of the actin-myosin interaction sites, which allows the muscle to contract.
- heparin in addition, before angioplasty or after a myocardial infarction or during certain treatments for cardiovascular conditions, patients are administered significant amounts of heparin and this treatment is often extended beyond 24 hours. Depending on the dose administered, the concentration of heparin in the plasma one hour after administration can vary between 0.1 to 2 IU / ml. The presence of heparin in the blood samples due to the treatment of the patient constitutes an important problem for the dosage of troponins. - 3 -
- the present invention relates more specifically to an immunological assay method for troponin I (cardiac or skeletal), T or C, and troponins I, T or C associated together in the form of dimers (IT, IC or TC) or in the form trimer (ITC) in a biological sample containing heparin, characterized in that the immunoassay is carried out in the presence of hexadimethrin bromide (polybrene).
- hexadimethrin bromide polybrene
- the amount of polybrene used during the immunoassay of troponin I (cardiac or skeletal), T or C, and troponins I, T or C associated with one another in the form of dimers (IT, IC or TC) or under Trimer form (ITC) may vary depending on the immunoassay procedure used.
- the ratio [molar concentration of the polybrene used / molar concentration of heparin present in the sample to be analyzed] can be from 1 to 1000. Depending on the dosage, it can more specifically be - 4 -
- the present invention can be implemented with any immunological method allowing the evaluation of troponin I (cardiac or skeletal), T or C, and troponins I, T or C associated with one another in the form of dimers (IT, IC or TC) or in the form of trimer (ITC).
- troponin I cardiac or skeletal
- T or C troponins I, T or C associated with one another in the form of dimers (IT, IC or TC) or in the form of trimer (ITC).
- troponin I cardiomyalpha-1 (cardiac or skeletal), T or C, and troponins I, T or C associated together in the form of dimers (IT, IC or TC) or in the form of trimer (ITC), to avoid interference due to heparin is part of the present invention
- heparin lithium heparinase I
- heparinase I heparinase I from Flavobacterium heparinum Sigma ref. H 2519
- L-histidine Sigma ref. H 8000
- protamine sulfate protamine sulfate grade X from salmon Sigma ref. P 4020
- antithrombin III antithrombin III from huma ⁇ plasma Sigma ref. A 7388
- cation exchange resins QSFF (Q Sepharose Fast Flow, Pharmacia Biotech ref. 17051001) and DEAE (DEAE Sepharose Fast Flow, Pharmacia Biotech ref. 17070901).
- the total analysis time is 15 minutes.
- the Troponine I Pasteur kit was used (Sanofi Diagnostics Pasteur, Marnes-la Coquette, France). The assay is carried out as follows:
- washing is carried out by adding 1 ml of 0.1 M phosphate buffer, pH 6.8 containing 0.1% Tween® 20 and 0.3% Kathon, keeping the temperature of the washing solution at most 8 ° C. This step is repeated 4 times.
- the enzymatic development is carried out using 600 ⁇ l of a mixture consisting of 1 part of tetramethylbenzydine and 100 parts of citrate buffer containing 4% DMSO and 0, 03% hydrogen peroxide.
- Tnl positive serum + diluent 1 160 1,240 Tnl positive serum + heparin 40IU / ml 0.480 1.170
- the troponin I assay is performed with the Troponine I Pasteur kit (Sanofi Diagnostics Pasteur, Marnes-la Coquette, France).
- the automated system used for the enzyme immunoassay is the Access® immuoassay system, a system marketed by Beckman.
- the immunoassay method is that described in Example I.
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9801367 | 1998-02-05 | ||
FR9801367A FR2774473B1 (fr) | 1998-02-05 | 1998-02-05 | Procede de dosage des troponines dans des milieux biologiques permettant d'eviter les interferences dues a l'heparine |
PCT/FR1999/000198 WO1999040442A1 (fr) | 1998-02-05 | 1999-02-01 | Dosage des troponines sans interferences dues a l'heparine |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1051623A1 true EP1051623A1 (de) | 2000-11-15 |
Family
ID=9522644
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99901673A Withdrawn EP1051623A1 (de) | 1998-02-05 | 1999-02-01 | Troponin-bestimmung ohne heparin-störung |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP1051623A1 (de) |
JP (1) | JP2002502979A (de) |
FR (1) | FR2774473B1 (de) |
WO (1) | WO1999040442A1 (de) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2002027316A2 (en) * | 2000-09-25 | 2002-04-04 | Abbott Laboratories | Methods and kits for decreasing interferences of assays samples containing plasma or serum in specific binding assays by using a large polycation |
WO2002063302A1 (en) * | 2001-02-07 | 2002-08-15 | Immunomatrix Inc. | A method for reducing heparin interference in diagnostic tests for cardiac troponin |
US20090317843A1 (en) * | 2006-12-22 | 2009-12-24 | Alberto Mantovani | Method for measuring plasma levels of long pentraxin ptx3 |
JP5334742B2 (ja) * | 2009-08-11 | 2013-11-06 | 関東化学株式会社 | 水溶性アンモニウムポリマーを含有する検体前処理試薬、および検体前処理方法 |
CN103380377B (zh) * | 2011-02-25 | 2016-01-27 | 美迪恩斯生命科技株式会社 | 心肌肌钙蛋白的测定方法 |
CN102426246A (zh) * | 2011-08-31 | 2012-04-25 | 内蒙古科慧生物科技有限责任公司 | 人肌钙蛋白I(Troponin I)定量测定试剂盒及其检测方法 |
CN104685359B (zh) * | 2012-07-31 | 2017-09-05 | 积水医疗株式会社 | 胶乳凝集抑制免疫测定 |
KR102528090B1 (ko) * | 2015-10-30 | 2023-05-02 | 가부시키가이샤 엘에스아이 메디엔스 | 트롬빈 안티트롬빈 복합체의 측정 시약 및 측정 방법 |
KR102228440B1 (ko) | 2016-04-13 | 2021-03-15 | 가부시키가이샤 엘에스아이 메디엔스 | 황산화 다당류를 이용한 면역학적 측정법 |
-
1998
- 1998-02-05 FR FR9801367A patent/FR2774473B1/fr not_active Expired - Fee Related
-
1999
- 1999-02-01 JP JP2000530804A patent/JP2002502979A/ja active Pending
- 1999-02-01 WO PCT/FR1999/000198 patent/WO1999040442A1/fr not_active Application Discontinuation
- 1999-02-01 EP EP99901673A patent/EP1051623A1/de not_active Withdrawn
Non-Patent Citations (1)
Title |
---|
See references of WO9940442A1 * |
Also Published As
Publication number | Publication date |
---|---|
FR2774473B1 (fr) | 2000-05-12 |
FR2774473A1 (fr) | 1999-08-06 |
WO1999040442A1 (fr) | 1999-08-12 |
JP2002502979A (ja) | 2002-01-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP3357163B2 (ja) | 免疫学的測定用トロポニン安定化組成物および安定化法 | |
JP7166364B2 (ja) | C反応性タンパク質を全域検出する方法及び対応するキット | |
EP0804736B1 (de) | Verfahren zur hochempfindlichen dosierung von herztroponin - i | |
JP3646097B2 (ja) | 前進性グリコシル化終末産物を含有しない特異的結合アッセイの試薬および固相成分 | |
EP1051623A1 (de) | Troponin-bestimmung ohne heparin-störung | |
JP2010513917A (ja) | ロングペントラキシンptx3の血漿レベルを測定するための方法 | |
WO2005124344A2 (fr) | Procede de detection d'anticorps anti-transglutaminase dans un echantillon de salive | |
US8900882B2 (en) | Method of assaying complex and kit to be used therefor | |
EP0063064B1 (de) | Reagens zur radio-oder enzymimmunologischen Bestimmung der IgE | |
FR2779526A1 (fr) | Nouveau procede de dosage de la troponine i cardiaque | |
EP0757795B1 (de) | Verfahren zum nachweis von endprodukten der fortgeschrittenen glykosylierung von hämoglobin | |
JP4877578B2 (ja) | 抗原の測定法およびそれに用いるキット | |
FR2709492A1 (fr) | Méthode d'immunodosage spécifique de la glutathion peroxydase plasmatique humaine, kit pour sa mise en Óoeuvre, oligopeptides et anticorps spécifiques de la méthode . | |
EP0164780A1 (de) | Verfahren zur immunologischen Bestimmung einer Substanz in einer flüssigen Probe | |
JP5294257B2 (ja) | ヘモグロビンの測定方法および測定用キット | |
JPH07198721A (ja) | 免疫測定用緩衝液 | |
JP4455688B2 (ja) | 分析物遊離段階を可能にするアッセイ用表面 | |
JP2000193666A (ja) | 吸収剤を用いた酵素免疫測定方法及び試薬 | |
FR3067814A1 (fr) | Procede d'application, sur un support solide, d'au moins un partenaire de liaison a une molecule | |
CA2210166C (fr) | Procede de dosage ultrasensible de la troponine i cardiaque | |
JPH0373857A (ja) | リウマチ患者のIgG上のガラクトース欠損を測定する方法 | |
CN113176407A (zh) | 一种定量检测Lp-PLA2含量的试剂盒 | |
EP0603061A1 (de) | Pyrogenentest und Reagenzsatz dafür | |
JP2023171257A (ja) | 交叉反応を抑制した葉酸測定方法 | |
FR3132356A1 (fr) | Méthode d’analyse de l’activation plaquettaire héparine dépendante |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20000801 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
|
GRAP | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOSNIGR1 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION HAS BEEN WITHDRAWN |
|
18W | Application withdrawn |
Effective date: 20031201 |