EP0991943A1 - Particules magnetiques avec des recepteurs biologiquement actifs - Google Patents

Particules magnetiques avec des recepteurs biologiquement actifs

Info

Publication number
EP0991943A1
EP0991943A1 EP97929133A EP97929133A EP0991943A1 EP 0991943 A1 EP0991943 A1 EP 0991943A1 EP 97929133 A EP97929133 A EP 97929133A EP 97929133 A EP97929133 A EP 97929133A EP 0991943 A1 EP0991943 A1 EP 0991943A1
Authority
EP
European Patent Office
Prior art keywords
magnetic particles
particles according
protein
magnetic
color pigments
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97929133A
Other languages
German (de)
English (en)
Inventor
Hans Lange
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Innova Gesellschaft Zur Entwicklung und Vermarktung Innovativer Produkte MBH
Original Assignee
Innova Gesellschaft Zur Entwicklung und Vermarktung Innovativer Produkte MBH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innova Gesellschaft Zur Entwicklung und Vermarktung Innovativer Produkte MBH filed Critical Innova Gesellschaft Zur Entwicklung und Vermarktung Innovativer Produkte MBH
Publication of EP0991943A1 publication Critical patent/EP0991943A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/5434—Magnetic particles using magnetic particle immunoreagent carriers which constitute new materials per se
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2446/00—Magnetic particle immunoreagent carriers
    • G01N2446/20—Magnetic particle immunoreagent carriers the magnetic material being present in the particle core
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/824—Immunological separation techniques
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing

Definitions

  • the invention relates to magnetic particles for separating biological mixtures, a process for their production and use of the magnetic particles.
  • US 4,795,698 discloses a method for producing suspendable magnetic particles coated with a biological material.
  • the coprecipitate is resuspended, washed and then processed into a magnetic polymer material.
  • the method has the disadvantage that iron ions are released ⁇ .
  • the object of the present invention is to eliminate the disadvantages of the prior art.
  • simple and inexpensive to produce magnetic particles for separating biological mixtures are to be provided.
  • magnetic particles are provided for separating biological mixtures, which have pearlescent color pigments with magnetic properties and a co-polymer coating.
  • Pearlescent color pigments are oily raw materials that are produced in particle form for the coating industry on a large scale. These are mica sheets that can be coated with various metal oxides, such as hamatite or titanium oxide.
  • black pigment In the case of magnetic pearlescent color pigments, a core made of mica is first coated with titanium oxide and then with magnetite. This so-called black pigment has ferromagnetic properties. It is available in m particle size distributions of usually 10-60 ⁇ m, preferably in m particle sizes of 1-30 ⁇ m.
  • the black pigment usually has the following composition:
  • the black pigment can surprisingly be coated directly with biological polymers. In this form it is suitable for separating biological mixtures.
  • magnétique pearlescent color pigments Another surprising property of the magnetic pearlescent color pigments is their resistance to acids, such as glacial acetic acid, alkalis, such as sodium hydroxide, and to inorganic and organic solvents, such as dimethyl sulfoxide / water mixtures, acetone, alcohols, nitro thinners, methylene chloride u. the like
  • acids such as glacial acetic acid, alkalis, such as sodium hydroxide
  • inorganic and organic solvents such as dimethyl sulfoxide / water mixtures, acetone, alcohols, nitro thinners, methylene chloride u. the like
  • the magnetic behavior of the particles according to the invention is not influenced by the aforementioned substances.
  • the particles according to the invention are preferably used in combinatorial chemistry and in high throughput screening. They can be used in particular as solid phase carriers for organic reactions.
  • the biological polymer coating is not resistant to reactions requiring extreme solvents. It has surprisingly been found that black pigments can be used as solid phase carriers in combinatorial chemistry or in high throughput screening. They can also be used as a catalyst.
  • R-IgG For the production of biotmylated Rmder immunoglobulin, 0.5 ml of an R-IgG solution, the 2 mg of R-IgG (Boeh ⁇ nger Mannneim) is dissolved, and 1 ml of phosphate-buffered saline solution, pH 7.25 (2.76 g / 1 NaH PO HO ; 3.56 g / 1 Na HPO 2H 0; 8 g / 1
  • the particles are then washed 3 times with 100 ml of Milli-Q water, the solid and liquid phase being separated between the washing steps by sedimentation or centrifugation.
  • the particles are then taken up again in 40 ml of phosphate-buffered sodium chloride solution, pH 7.25.

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

Les particules magnétiques décrites, qui servent à séparer des mélanges biologiques, sont constituées de pigments brillants nacrés à propriétés magnétiques et d'un enduit polymère biologique.
EP97929133A 1997-06-18 1997-06-18 Particules magnetiques avec des recepteurs biologiquement actifs Withdrawn EP0991943A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/DE1997/001300 WO1998058257A1 (fr) 1997-06-18 1997-06-18 Particules magnetiques avec des recepteurs biologiquement actifs

Publications (1)

Publication Number Publication Date
EP0991943A1 true EP0991943A1 (fr) 2000-04-12

Family

ID=6918506

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97929133A Withdrawn EP0991943A1 (fr) 1997-06-18 1997-06-18 Particules magnetiques avec des recepteurs biologiquement actifs

Country Status (4)

Country Link
US (1) US6372517B1 (fr)
EP (1) EP0991943A1 (fr)
JP (1) JP2002511141A (fr)
WO (1) WO1998058257A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10355409A1 (de) 2003-11-25 2005-06-30 Magnamedics Gmbh Sphärische, magnetische Silicagel-Träger mit vergrößerter Oberfläche für die Aufreinigung von Nukleinsäuren
JP3922648B2 (ja) 2004-05-24 2007-05-30 株式会社資生堂 アフィニティー粒子及びアフィニティー分離方法
US8349067B2 (en) 2006-11-09 2013-01-08 Sun Chemical Corp. Multi-colored lustrous pearlescent pigments
US8221536B2 (en) 2006-11-09 2012-07-17 Sun Chemical Corp. Cosmetic comprising multi-colored lustrous pearlescent pigments
US8906154B2 (en) * 2006-11-09 2014-12-09 Sun Chemical Corporation Coating, ink, or article comprising multi-colored lustrous pearlescent pigments
US8323396B2 (en) * 2006-11-09 2012-12-04 Sun Chemical Corp. Orange pearlescent pigments
US7850775B2 (en) * 2006-11-09 2010-12-14 Sun Chemical Corporation Multi-colored lustrous pearlescent pigments
US8211224B2 (en) * 2006-11-09 2012-07-03 Sun Chemical Corp. Multi-colored lustrous pearlescent pigments and process for making
US8211225B2 (en) 2008-04-09 2012-07-03 Sun Chemical Corp. Magnetic pigments and process of enhancing magnetic properties
DE102010014840B4 (de) 2010-04-13 2015-12-10 Magnamedics Gmbh DNase-beschichtete magnetische Partikel

Family Cites Families (31)

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US4038099A (en) * 1971-08-30 1977-07-26 The Mearl Corporation Rutile-coated mica nacreous pigments and process for the preparation thereof
US3861946A (en) * 1972-07-21 1975-01-21 Mearl Corp Titanium dioxide nacreous pigments and process for the preparation thereof
DE2313331C2 (de) * 1973-03-17 1986-11-13 Merck Patent Gmbh, 6100 Darmstadt Eisenoxidhaltige Glimmerschuppenpigmente
US3970518A (en) 1975-07-01 1976-07-20 General Electric Company Magnetic separation of biological particles
GB1556272A (en) * 1976-05-26 1979-11-21 Mearl Corp Iron oxide coated mica nacreous pigments
US4055377A (en) * 1976-08-03 1977-10-25 Minnesota Mining And Manufacturing Company Magnetically orientable retroreflectorization particles
US4192691A (en) * 1978-10-26 1980-03-11 The Mearl Corporation Metal oxide platelets as nacreous pigments
DE3030056A1 (de) * 1980-08-08 1982-03-25 Basf Ag, 6700 Ludwigshafen Verfahren zur herstellung von mit metalloxiden beschichteten schuppenfoermigen glimmerpigmenten
US4725499A (en) * 1982-06-24 1988-02-16 Mitsui Toatsu Chemicals, Inc. Polymer-coated solid materials
US4795698A (en) 1985-10-04 1989-01-03 Immunicon Corporation Magnetic-polymer particles
US4868106A (en) * 1985-10-17 1989-09-19 Konishiroku Photo Industry Co., Ltd. Analytical element and method for determining a component in a test sample
US5705265A (en) * 1986-03-24 1998-01-06 Emsci Inc. Coated substrates useful as catalysts
DE3617430A1 (de) * 1986-05-23 1987-11-26 Merck Patent Gmbh Perlglanzpigmente
DE3709217A1 (de) * 1987-03-20 1988-09-29 Basf Ag Plaettchenfoermige pigmente auf basis eisenoxid
FI77881C (fi) * 1987-04-14 1989-05-10 Kemira Oy Ytbelagda silikatpartiklar.
FI78924C (fi) * 1988-01-22 1989-10-10 Kemira Oy Faergaemnes- eller faergpigmenthaltigt paerlemopigment och ett foerfarande foer dess framstaellning.
CA1329867C (fr) * 1988-07-20 1994-05-31 Hiroshi Ito Pigment
JP2979414B2 (ja) * 1989-09-29 1999-11-15 富士レビオ株式会社 磁性粒子およびそれを用いた免疫測定法
DE3938055A1 (de) * 1989-11-16 1991-05-23 Merck Patent Gmbh Mit plaettchenfoermigen pigmenten beschichtete materialien
US5935866A (en) * 1989-12-22 1999-08-10 Binax Nh, Inc. Preparation of sub 100 A magnetic particles and magnetic molecular switches
AU8951191A (en) * 1990-10-29 1992-05-26 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
CA2114913C (fr) * 1993-02-05 2003-12-09 Takafumi Atarashi Poudre presentant au moins une couche, et procede servant a la preparer
US5855790A (en) * 1994-02-07 1999-01-05 Selective Environmental Technologies, Inc. Magnetic particles, a method for the preparation thereof and their use in the purification of solutions
US5643721A (en) * 1994-02-09 1997-07-01 Abbott Laboratories Bioreagent immobilization medium
US5576185A (en) * 1994-04-15 1996-11-19 Coulter Corporation Method of positive or negative selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation
US5698839A (en) * 1995-04-07 1997-12-16 Eastman Kodak Company Magnetically encodable card having magnetic pigment uniformly dispersed in plastic
DE19606598C1 (de) * 1996-02-22 1997-11-13 Innova Gmbh Magnetpartikel mit biologisch aktiven Rezeptoren sowie Herstellung und Verwendung
US6045914A (en) * 1996-06-03 2000-04-04 Engelhard Corporation Pearlescent glass pigment
WO1999018438A1 (fr) * 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Analyses capillaires impliquant la separation d'especes libres et liees
US6001526A (en) * 1998-03-24 1999-12-14 Minolta Co., Ltd. Binder carrier containing ethylenic unsaturated nitrile copolymer as binder resin
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Non-Patent Citations (1)

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Title
See references of WO9858257A1 *

Also Published As

Publication number Publication date
WO1998058257A1 (fr) 1998-12-23
JP2002511141A (ja) 2002-04-09
US6372517B1 (en) 2002-04-16

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