Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
  • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
  • C07D417/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/04—Drugs for disorders of the alimentary tract or the digestive system for ulcers, gastritis or reflux esophagitis, e.g. antacids, inhibitors of acid secretion, mucosal protectants
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
  • A61P1/12—Antidiarrhoeals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/18—Antipsychotics, i.e. neuroleptics; Drugs for mania or schizophrenia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
  • C07D413/10—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a carbon chain containing aromatic rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings

Definitions

• the present invention relates to novel compounds which are potent and specific antagonists of 5-hydroxytryptamine (5- HT;serotonin).
• the present invention provides substituted phenylcarbamates and ureas of formula (I): wherein R 1 represents a hydrogen or a halogen atom, or a C 1-6 alkyl, C 1-6 alkoxy or hydroxy group;
• Suitable pharmaceutically acceptable salts of the compounds of general formula (I) include acid addition salts formed with pharmaceutically acceptable organic or inorganic acids for example, hydrochlorides, hydrobromides, sulphates, alkyl- or arylsulphonates (e.g. methanesulphonates or p -toluenesulphonates), phosphates, acetates, citrates, succinates, tartrates, fumarates and maleates.
• pharmaceutically acceptable organic or inorganic acids for example, hydrochlorides, hydrobromides, sulphates, alkyl- or arylsulphonates (e.g. methanesulphonates or p -toluenesulphonates), phosphates, acetates, citrates, succinates, tartrates, fumarates and maleates.
• solvates may, for example, be hydrates.
• references hereafter to a compound according to the invention includes both compounds of formula (I) and their quaternary ammonium derivatives, piperidine N-oxides and pharmaceutically acceptable acid addition salts together with pharmaceutically acceptable solvates.
• Quaternary ammonium derivatives of compounds of formula (I) are compounds of formula where Q represents C 1-6 alkyl (e.g. methyl).
• Piperidine N-oxides of compounds of formula (I) are compounds of formula
• the oxadiazole or thiadiazole ring R 2 in the compounds of formula (I) may be a 1,2,4-oxadiazol-5-yl or -3-yl or 1,2,4-thiadiazol-5-yl or -3-yl, i.e. where W is -O- or -S-.
• a C 1-6 alkyl group may be a straight chain or branched chain alkyl group, for example, methyl, ethyl, propyl, prop-2-yl, butyl, but-2-yl, 2-methylprop-2-yl, pentyl, pent-3-yl or hexyl.
• a -CH 2 C 2-5 alkenyl group may be, for example, a propenyl or butenyl group.
• a -CH 2 C 2-5 alkynyl group may be, for example, a prop-2-ynyl group.
• C 3-7 cycloalkyl may be, for example, cyclopropyl, cyclobutyl or cyclohexyl.
• R 1 represents a halogen atom this may be, for example, a fluorine, chlorine, bromine or iodine atom.
• R 1 may be attached at any vacant position on the phenyl ring.
• R 1 may be para to the -NH- substituent or para to R 2 .
• a preferred class of compounds of formula (I) is that in which R 1 represents a hydrogen atom, a halogen (e.g. fluorine) atom or a C 1-6 alkyl (e.g. methyl) or C 1-6 alkoxy (e.g. methoxy) group. Furthermore, when R 1 represents a halogen (e.g. fluorine) atom or a C 1-6 alkyl (e.g. methyl) or C 1-6 alkoxy (e.g. methoxy) group this is preferably attached para to the -NH- substituent or para to R 2 .
• R 1 represents a hydrogen atom, a halogen (e.g. fluorine) atom or a C 1-6 alkyl (e.g. methyl) or C 1-6 alkoxy (e.g. methoxy) group.
• R 2 represents an oxadiazole or thiadiazole ring substituted by a C 1-6 alkyl(e.g.methyl, prop-2-yl or butyl), C 3- 7 cycloalkyl(e.g.cyclopropyl), phenyl or benzyl group, for example a 1,2,4-oxadiazol-5-yl substituted in the 3-position by a C 1-6 alkyl (e.g.
• C 3-7 cycloalkyl e.g.cyclopropyl
• phenyl or benzyl group or 1,2,4-oxadiazol-3-yl substituted in the 5-position by a C 1-6 alkyl(e.g.methyl) group or a 1,2,4-thiadiazol-5- yl substituted in the 3-position by a C 1-6 alkyl (e.g. methyl) group.
• a preferred class of compounds of formula (I) is that in which R 3 represents C 1-6 alkyl (e.g. prop-2-yl), benzyl, (where R 5 preferably represents SO 2 R 8 , e.g. SO 2 Me) or, more preferably, -(CH 2 ) n R 4 .
• a preferred class of compounds of formula (I) is that in which n represents 3 or, more preferably, 2 and R 4 represents a -C 1-6 alkoxy (e.g. methoxy), -CONR 6 R 7 (e.g. CONH 2 ), -NR 6 COR 7 (e.g. NHCOMe), -SO 2 NR 6 R 7 (e.g. SO 2 NHMe) or - NR 6 SO 2 R 7 (e.g. NHSO 2 Me or NMeSO 2 Me) group.
• n represents 3 or, more preferably, 2 and R 4 represents a -C 1-6 alkoxy (e.g. methoxy), -CONR 6 R 7 (e.g. CONH 2 ), -NR 6 COR 7 (e.g. NHCOMe), -SO 2 NR 6 R 7 (e.g. SO 2 NHMe) or - NR 6 SO 2 R 7 (e.g. NHSO 2 Me or NMeSO 2 Me) group.
• a further preferred class of compounds of formula (I) is that in which m represents 1.
• a preferred group of compounds of formula (I) is that in which R 1 represents a hydrogen or halogen (e.g. fluorine) atom, a C 1-6 alkyl (e.g. methyl) or C 1-6 alkoxy (e.g. methoxy) group (e.g. a hydrogen atom or a fluorine atom or methyl or methoxy group para to the -NH- substituent or para to R 2 ); R 2 represents an oxadiazole or thiadiazole ring substituted by a C 1-6 alkyl (e.g. methyl, prop-2-yl or butyl), C 3-7 cycloalkyl (e.g.
• R 1 represents a hydrogen or halogen (e.g. fluorine) atom, a C 1-6 alkyl (e.g. methyl) or C 1-6 alkoxy (e.g. methoxy) group (e.g. a hydrogen atom or a fluorine atom or methyl or methoxy
• cyclopropyl phenyl or benzyl group
• X represents NH or, more preferably, an oxygen atom
• m represents zero, 2 or, more preferably, 1
• R 3 represents C 1-6 alkyl (e.g. prop-2-yl), benzyl, (where R 5 preferably represents SO 2 R 8 , e.g. SO 2 Me) or, more preferably, -(CH 2 ) n R 4 where n represents 3 or, more preferably, 2 and R 4 represents C 1-6 alkoxy (e.g. methoxy), -CONR 6 R 7 (e.g.
• the compounds of the invention are antagonists of 5-HT both in vitro and in vivo and are thus of use in the treatment of conditions mediated by 5-HT.
• the compounds of the invention inhibit the 5-HT induced contraction of guinea-pig colon (essentially following the general procedure described by C.J. Elswood et.al in Br. J. Pharmac., 1990, 100 , (Proc.Suppl.) 485P and Eur. J. Pharmac., 1991, 196 , 149-155 in the presence of ondansetron and methysergide) and the 5-HT-induced secretion in rat colon (as described by K T Dunce et.al in Br.J. Pharmac., 1991, 102 , 811-816), and are thus useful in the treatment of 5-HT mediated disorders in which there is a disturbance of gastrointestinal function.
• Conditions involving disturbance of intestinal function include for example irritable bowel syndrome and its associated pain, excessive gastrointestinal secretion, and/or diarrhoea for example diarrhoea associated with excessive gastrointestinal secretion, cholera infection and carcinoid syndrome.
• the compounds of the invention may also be useful in the treatment of emesis.
• the compounds of the invention have been shown to be 5-HT 4 antagonists in vitro as demonstrated by their ability to inhibit the 5-HT-induced relaxation of the rat oesophagus preparation (essentially following the general procedure described by J J Reeves et al in Br. J Pharmac., 1989, 98 , (Proc.Suppl.), 800P and 1991, 103 , 1067-1072) and are thus of use in the treatment of conditions capable of amelioration by antagonism of such receptors.
• 5-HT 4 receptors have been found in, for example, the digestive and urinary tracts, brain and cardiovascular system of mammals, including man, and are thus believed to be associated with conditions involving the digestive and urinary tracts (e.g. urinary incontinence), cardiovascular system and CNS disorders.
• the compounds of the invention may also be useful in the treatment of movement disorders (e.g. Parkinsonism), psychoses such as schizophrenia, mania, dementia or other cognitive disorders e.g. Alzheimer's disease; depression; and dependency on drugs or substances of abuse.
• movement disorders e.g. Parkinsonism
• psychoses such as schizophrenia, mania, dementia or other cognitive disorders e.g. Alzheimer's disease; depression; and dependency on drugs or substances of abuse.
• the compounds of the invention have been shown to be active in the rat social interaction test as described by B J Jones et al in Br J Pharmac., 1988, 93 , 985-93 and are thus of use in the treatment of anxiety.
• the invention therefore provides a compound of formula (I) or a quaternary ammonium derivative, piperidine N- oxide or a pharmaceutically acceptable salt or solvate thereof for use in therapy, in particular in human medicine. It will be appreciated that use in therapy embraces but is not necessarily limited to use of a compound of the invention as an active therapeutic substance.
• a method for the treatment of a mammal comprising administration of an effective amount of a compound of formula (I) or a quaternary ammonium derivative, piperidine N-oxide or a pharmaceutically acceptable salt or solvate thereof in particular in the treatment of conditions mediated by 5-hydroxytryptamine, in particular conditions capable of amelioration by antagonism of 5-HT 4 receptors.
• the invention also provides a pharmaceutical composition
• a pharmaceutical composition comprising a compound of formula (I) or a quaternary ammonium derivative, piperidine N-oxide or a pharmaceutically acceptable salt or solvate thereof and formulated for administration by any convenient route.
• Such compositions are preferably in a form adapted for use in medicine, in particular human medicine, and can conveniently be formulated in conventional manner using pharmaceutically acceptable carriers.
• the compounds according to the invention may be formulated for oral, buccal, parenteral, topical, implant or rectal administration or in a form suitable for administration by inhalation or insufflation (either through the mouth or nose).
• the pharmaceutical compositions may take the form of, for example, tablets or capsules prepared by conventional means with pharmaceutically acceptable excipients such as binding agents (e.g. pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose); fillers (e.g. lactose, microcrystalline cellulose or calcium hydrogen phosphate); lubricants (e.g. magnesium stearate, talc or silica); disintegrants (e.g. potato starch or sodium starch glycollate); or wetting agents (e.g. sodium lauryl sulphate).
• binding agents e.g. pregelatinised maize starch, polyvinylpyrrolidone or hydroxypropyl methylcellulose
• fillers e.g. lactose, microcrystalline cellulose or calcium hydrogen phosphate
• lubricants e.g. magnesium stearate, talc or silica
• disintegrants e.g. potato starch or sodium starch glycollate
• Liquid preparations for oral administration may take the form of, for example, solutions, syrups or suspensions, or they may be presented as a dry product for constitution with water or other suitable vehicle before use.
• Such liquid preparations may be prepared by conventional means with pharmaceutically acceptable additives such as suspending agents (e.g. sorbitol syrup, cellulose derivatives or hydrogenated edible fats); emulsifying agents (e.g. lecithin or acacia); non-aqueous vehicles (e.g. almond oil, oily esters, ethyl alcohol or fractionated vegetable oils); and preservatives (e.g. methyl or propyl- p -hydroxybenzoates or sorbic acid).
• the preparations may also contain buffer salts, flavouring, colouring and sweetening agents as appropriate.
• Preparations for oral administration may be suitably formulated to give controlled release of the active compound.
• Preparations for oral administration may also be suitably formulated to give "pulsed release” i.e. rapid release of the active ingredient after an initial time delay.
• pulsed release formulations may further be enterically coated to allow targeting of drugs to the colon for either a direct local action, or to provide a preferred site for drug delivery
• compositions may take the form of tablets or lozenges formulated in conventional manner.
• the compounds of the invention may be formulated for parenteral administration by bolus injection or continuous infusion.
• Formulations for injection may be presented in unit dosage form e.g. in ampoules or in multi-dose containers, with an added preservative.
• the compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising and/or dispersing agents.
• the active ingredient may be in powder form for constitution with a suitable vehicle, e.g. sterile pyrogen-free water, before use.
• the compounds of the invention may also be formulated in rectal compositions such as suppositories or retention enemas, e.g. containing conventional suppository bases such as cocoa butter or other glycerides.
• the compounds of the invention may also be formulated as depot preparations. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection.
• the compounds of the invention may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.
• the compounds according to the invention may be formulated as solutions or suspensions for administration via a suitable metered or unit dose device or alternatively as a powder mix with a suitable carrier for administration using a suitable delivery device.
• the compounds according to the invention may be formulated as solutions, suspensions, creams or ointments and be included in systems that provide controlled release.
• a proposed dose of the compounds of the invention for administration to man is 1mg to 100mg, of the active ingredient per unit dose expressed as the weight of free base, which could be administered, for example, 1 to 4 times per day. It will be appreciated that it may be necessary to make routine variations to the dosage, depending on the age and condition of the patient and the precise dosage will be ultimately at the discretion of the attendant physician or veterinarian. The dosage will also depend on the route of administration.
• the compounds of the invention may, if desired, be administered in combination with one or more other therapeutic agents and formulated for administration by any convenient route in a conventional manner. Appropriate doses will be readily appreciated by those skilled in the art.
• a compound of formula (I) may be prepared by reacting a compound of formula (II) or (III): or a protected derivative thereof, wherein Y represents a leaving atom or group such as a halogen (e.g. chlorine) atom, with a compound of formula (IV): where M is NH 2 , OH or an activated derivative thereof(e.g. an alkali metal (e.g. lithium) alkoxide), optionally in the presence of a strong acid such as methanesulphonic acid.
• a compound of formula (I) may be prepared by reacting a compound of formula (II) or (III): or a protected derivative thereof, wherein Y represents a leaving atom or group such as a halogen (e.g. chlorine) atom, with a compound of formula (IV): where M is NH 2 , OH or an activated derivative thereof(e.g. an alkali metal (e.g. lithium) alkoxide), optionally in the presence of a
• the reaction is conveniently effected in an inert organic solvent such as an ether (e.g. tetrahydrofuran) or a halogenated hydrocarbon (e.g. dichloromethane) at a temperature between -80°C and the reflux temperature of the solvent.
• an inert organic solvent such as an ether (e.g. tetrahydrofuran) or a halogenated hydrocarbon (e.g. dichloromethane) at a temperature between -80°C and the reflux temperature of the solvent.
• the reaction may take place by fusion in the absence of any solvent at an elevated temperature such as 100-200°C, e.g. 140 to 175°C.
• a leaving atom or group such as a halogen (e.g. chlorine, bromine or iodine) atom, or an acyloxy (e.g. trifluoroacetyloxy) or a sulphonyloxy (e.g. p -toluenesulphonyloxy) group and
• the reaction is conveniently effected in an inert organic solvent such as acetonitrile, a substituted amide (e.g. dimethylformamide) or an aromatic hydrocarbon (e.g. toluene), at an elevated temperature, for example at the reflux temperature of the solvent.
• an inert organic solvent such as acetonitrile, a substituted amide (e.g. dimethylformamide) or an aromatic hydrocarbon (e.g. toluene)
• Suitable reducing agents include borohydrides such as sodium cyanoborohydride or sodium triacetoxyborohydride.
• Suitable solvents will depend upon the reducing agent used, but may include alcohols, for example, methanol or ethanol, halogenated hydrocarbons, for example, 1,2-dichloroethane or ethers, for example, diethyl ether or tetrahydrofuran. The reaction conveniently takes place at ambient temperature.
• a compound of formula (I) where R 2 is a 1,2,4-oxadiazol-3-yl group may be prepared by reacting a compound of formula (VII) where Z is C 1-6 alkoxy (e.g. methoxy), with a compound of formula (VIII) where R 9 is a substituent as defined for R 2 , in the presence of a strong base such as sodium hydride.
• the reaction conveniently takes place in a suitable solvent such as an ether (e.g. tetrahydrofuran) at an elevated temperature, such as the reflux temperature of the reaction mixture.
• a suitable solvent such as an ether (e.g. tetrahydrofuran)
• an elevated temperature such as the reflux temperature of the reaction mixture.
• a compound of formula (I) where R 2 is a 1,2,4-thiadiazol-5-yl group may be prepared by reacting a compound of formula (IX) where R 9 is as defined above, with hydroxylamine-O-sulfonic acid, optionally in the presence of a base such as pyridine.
• the reaction conveniently takes place in a suitable solvent such as an alcohol (e.g. methanol) at ambient temperature.
• a suitable solvent such as an alcohol (e.g. methanol) at ambient temperature.
• a compound of formula (I), where R 3 represents a group -(CH 2 ) n R 4 where R 4 is a group NH-SO 2 R 7 may be prepared by reacting a compound of formula (X) with a compound (Xl) R 7 SO 2 -hal where hal is a halogen (e.g. chlorine) atom, in the presence of a base (e.g. pyridine).
• a compound of formula (X) with a compound (Xl) R 7 SO 2 -hal where hal is a halogen (e.g. chlorine) atom, in the presence of a base (e.g. pyridine).
• reaction conveniently takes place in a suitable solvent such as a halogenated hydrocarbon (e.g. dichloromethane) at ambient temperature
• a suitable solvent such as a halogenated hydrocarbon (e.g. dichloromethane) at ambient temperature
• a compound of formula (I) may be converted into another compound of formula (I) using conventional techniques.
• compounds of formula (I) where R 3 represents a group containing an -NH- moiety may be convened into another compound of formula (I) wherein R 3 contains a -N(C 1-6 alkyl)- moiety (e.g. -N(CH 3 )-) using a suitable alkylating agent such as an alkyliodide e.g. methyliodide) as described above.
• a suitable alkylating agent such as an alkyliodide e.g. methyliodide
• the reaction conveniently takes place in a suitable solvent such as an ether (e.g. tetrahydrofuran) at ambient temperature and in the presence of a strong base such as sodium hydride.
• quaternary ammonium salts of compounds of formula (I) may be prepared by reacting non-quaternary compounds of formula (I) with a suitable quaternising agent such as Q-L (where L is a leaving group as defined above e.g. a halogen (e.g. iodine) atom and Q is as defined above).
• a suitable quaternising agent such as Q-L (where L is a leaving group as defined above e.g. a halogen (e.g. iodine) atom and Q is as defined above).
• Q-L where L is a leaving group as defined above e.g. a halogen (e.g. iodine) atom and Q is as defined above.
• a suitable solvent such as a chlorinated hydrocarbon (e.g. chloroform) at ambient temperature.
• Piperidine N-oxides of compounds of formula (I) may be prepared by reacting an appropriate piperidine compound of formula (I) with a suitable oxidising agent such as 3-chloro peroxybenzoic acid. Oxidation conveniently takes place in a suitable solvent such as a halogenated hydrocarbon (e.g. chloroform) at ambient temperature.
• a suitable oxidising agent such as 3-chloro peroxybenzoic acid. Oxidation conveniently takes place in a suitable solvent such as a halogenated hydrocarbon (e.g. chloroform) at ambient temperature.
• R 3 represents a hydroxyl group it may be necessary to protect the hydroxyl group, for example with an arylmethyl (e.g. benzyl or trityl) group. It may also be necessary to protect compounds where R 3 contains other sensitive groups such as an amine group. Such groups may be protected for example using an acyl group (e.g. benzyloxycarbonyl) or a silyl group (e.g. trimethylsilyl).
• acyl group e.g. benzyloxycarbonyl
• silyl group e.g. trimethylsilyl
• a compound of general formula (I) may be prepared by the removal of any protecting groups from a protected form of a compound of formula (I). Deprotection may be effected using conventional techniques such as those described in 'Protective Groups in Organic Synthesis 2nd Ed.' by T. W. Greene and P G M Wuts (John Wiley and Sons, 1991).
• Compounds of formula (III) are either known or may be prepared from known compounds by conventional procedures.
• the compounds may be prepared by treatement of the corresponding aniline of formula (XII) with phosgene followed by a strong base such as triethylamine.
• Compounds of formula (V) may be prepared, for example, by reacting a compound of formula (XIII) wherein R 10 is a C 1-6 alkyl (e.g. methyl) group with a chloroformate (e.g. 1-chloroethyl chloroformate, vinylchloroformate or ethylchloroformate) at an elevated temperature, with subsequent heating of the reaction mixture at reflux temperature with an alcohol (e.g. methanol).
• a chloroformate e.g. 1-chloroethyl chloroformate, vinylchloroformate or ethylchloroformate
• Compounds of formula (XII) may be prepared according to the method of general process (A), by reacting a compound of formula (II) with a compound of formula (XIV) where M is as defined above and R 11 is a C 1-6 alkyl (e.g. methyl) group.
• Compounds of formula (VII) may be prepared by reacting compounds of formula (XV) or (XVI) where Y and Z are as defined above, with a compound of formula (IV) as defined above, under conditions as described above for process (A).
• Compounds of formula (IX) may be prepared by reacting a compound of formula (XVII) with a compound of formula (XVIII) R 9 C(OCH 3 ) 2 N(CH 3 ) 2 where R 9 is as defined above.
• the reaction conveniently takes place in a suitable solvent such as an amide (e.g. dimethylformamide) at ambient temeprature.
• a suitable solvent such as an amide (e.g. dimethylformamide) at ambient temeprature.
• Compounds of formula (XVII) may be prepared by reacting compounds of formulae (XIX) or (XX) or a protected derivative thereof, where Y is as defined above, with a compound of formula (IV) as defined above, under conditions as described above for process (A), followed by removal of any protecting groups present.
• Compounds of formula (K) may be prepared by reacting a compound of formula (V) with an appropriate alkylating agent under conditions as described in process (B).
• compounds of formula (IV) may be prepared by reduction of the corresponding compounds of formula (XXI) wherein A - represents an associated anion such as a halide (e.g. bromide) anion and M is as defined above.
• Reduction may be conveniently effected by hydrogenation in the presence of a suitable catalyst, such as rhodium on alumina, in the presence of a suitable solvent, for example under aqueous conditions.
• Compounds of formula (XXI) may be prepared by alkylation of 4- pyridine methanol or 4-pyridine methylamine (in which the amino group has been protected with a suitable protecting group) using a suitable alkylating agent of formula (VIa) as defined hereinbefore.
• the reaction conveniently takes place in the presence of sodium iodide in a suitable solvent such as an alcohol (e.g. isopropanol) at the reflux temperature of the solvent.
• a compound of the invention for example a physiologically acceptable salt
• this may be achieved by reacting the compound of formula (I) in the form of the free base with an appropriate acid, preferably with an equivalent amount, in a suitable solvent such as an alcohol (e.g. ethanol or methanol), an ester (e.g. ethyl acetate) or an ether (e.g. tetrahydrofuran).
• a suitable solvent such as an alcohol (e.g. ethanol or methanol), an ester (e.g. ethyl acetate) or an ether (e.g. tetrahydrofuran).
• Physiologically acceptable salts may also be prepared from other salts, including other physiologically acceptable salts, of the compound of formula (I) using conventional methods.
• the compounds of the invention may readily be isolated in association with solvent molecules by crystallisation from or evaporation of an appropriate solvent to give the corresponding solvates.
• Individual enantiomers of the compounds of the invention may be obtained by resolution of a mixture of enantiomers (e.g a racemic mixture) using conventional means, such as an optically active resolving acid; see for example Stereochemistry of Carbon Compounds' by E.L.Eliel (McGraw Hill, 1962) and 'Tables of Resolving Agents' by S. H. Wilen.
• optically active resolving acid see for example Stereochemistry of Carbon Compounds' by E.L.Eliel (McGraw Hill, 1962) and 'Tables of Resolving Agents' by S. H. Wilen.
• N-[2-[[[1-(Dimethylamino)ethylidene]amino]thioxomethyl] phenyl]acetamide (5.1g) and hydroxylamine-o-sulfonic acid (3.048g) were dissolved in methanol (60ml). Pyridine (2.9ml) was added and the resulting solution was stirred under nitrogen at room temperature for 24h. Concentration in vacuo gave a brown solid. This was separated between sodium carbonate solution (60ml) and dichloromethane (50ml), the aqueous extracted with fresh dichloromethane (5x30ml) and the combined organic extracts dried.
• N-[2-(3-Methyl-1,2,4-thiadiazol-5-yl)phenyl]acetamide (1.487g) was dissolved in Claisen's alkali (6.3M KOH in methanol, 12ml) and refluxed under a nitrogen atmosphere for 16h, then cooled and extracted with ethyl acetate (3x40ml). The combined organic extracts were washed with sodium chloride solution (10ml), dried and concentrated in vacuo to give an orange powder (1.1g). FCC using cyclohexane:ethyl acetate (4:1) as eluant gave the title compound as a yellow solid, (302mg).T.l.c. (cyclohexane:ethyl:acetate, 4:1) Rf 0.6
• T.l.c. (System A, 200:8: 1) Rf 0.25 From 4-methyl-2-(3-methyl-1,2,4-oxadiazol-5-yl)benzenamine (600mg) and N-[2-[4-(hydroxymethyl)-1-piperidinyl]ethyl]methanesulphonamide (790mg).
• Water analysis contains 0.35% H 2 O by weight.
• N-[2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl]-N'-[2-(4-piperidinyl)ethyl]urea (450mg) (287mg), analar grade acetone (0.075ml) and glacial acetic acid (0.07ml) were stirred in dichloromethane (20ml) under nitrogen.
• Sodium triacetoxyborohydride (269mg) was cautiously added. The cloudy liquid was stirred and after 49h a further quantity of acetone (0.05ml) was added. After 68h sodium triacetoxyborohydride (100mg) was added. After 120h 8% sodium bicarbonate solution was added.
• the aqueous was are-basified with sodium bicarbonate (pH8) and the product extracted into dichloromethane (3x500ml). The solvent was removed under vacuo yielding a white solid which was further purified by FCC with 15% methanol in toluene as eluent, giving the title compound as a pale yellow solid (1.53g), m.p.105-107°. T.l.c. (15% methanol in toluene) Rf 0.74
• Methanesulphonyl chloride (0.02ml) was added to a stirred solution of 1-[(2-aminoethyl-4-piperidinyl]methyl[2-(3-methyl-1,2,4-oxadiazol-5-yl)phenyl] carbamate (100mg) and pyridine (0.05ml) in dry dichloromethane (2.5ml) under nitrogen. After 1.5h water (10ml) was added and the mixture extracted with dichloromethane (3x5ml). The combined extracts were dried, filtered and evaporated to give a yellow oil ( ⁇ 125mg). FCC with System A (250:10:1) as the eluent gave the title compound as a white solid (68mg) m.p. 126-7°.
• the compounds of the invention exhibited no apparent adverse or toxic effects when administered to rats in vivo .
• the compound of Example 1 exhibited no apparent adverse or toxic effects when administered intraperitoneally to conscious rats up to doses of 1mg/kg.
• the compound of the invention, microcrystalline cellulose, lactose and cross linked polyvinylpyrrolidone are sieved through a 500 micron sieve and blended in a suitable mixer.
• the magnesium stearate is sieved through a 250 micron sieve and blended with the active blend.
• the blend is compressed into tablets using suitable punches.
• Compound of the invention 5.0mg Lactose 165.0mg Pregelatinised Starch 20.0mg
• Cross-linked polyvinylpyrrolidone 8.0mg Magnesium Stearate 2.0mg Compression weight 200.0mg
• the compound of the invention, lactose and pregelatinised starch are blended together and granulated with water.
• the wet mass is dried and milled.
• the magnesium stearate and cross-linked polyvinylpyrrotidone are screened through a 250 micron sieve and blended with the granule.
• the resultant blend is compressed using suitable tablet punches.
• the compound of the invention and pregelatinised starch are screened through a 500 micron mesh sieve, blended together and lubricated with magnesium stearate, (meshed through a 250 micron sieve). The blend is filled into hard gelatine capsules of a suitable size.
• Compound of the invention 5.0mg Lactose 177.0mg Polyvinylpyrrolidone 8.0mg Cross-linked polyvinylpyrrolidone 8.0mg Magnesium Stearate 2.0mg Fill weight 200.0mg
• the compound of the invention and lactose are blended together and granulated with a solution of polyvinylpyrrolidone.
• the wet mass is dried and milled.
• the magnesium stearate and cross-linked polyvinylpyrrolidone are screened through a 250 micron sieve and blended with the granules.
• the resultant blend is filled into hard gelatine capsules of a suitable size.
• the hydroxypropyl methylcellulose is dispersed in a portion of hot purified water together with the hydroxybenzoates and the solution is allowed to cool to room temperature.
• the saccharin sodium flavours and sorbitol solution are added to the bulk solution.
• the compound of the invention is dissolved in a portion of the remaining water and added to the bulk solution. Suitable buffers may be added to control the pH in the region of maximum stability.
• the solution is made up to volume, filtered and filled into suitable containers.
• % w/v Compound of the invention 1.00 Water for injections B.P. to 100.00
• Sodium chloride may be added to adjust the tonicity of the solution and the pH may be adjusted to that of maximum stability and/or to facilitate solution of the compound of the invention using dilute acid or alkali or by the addition of suitable buffer salts.
• Antioxidants and metal chelating salts may also be included.
• the solution is prepared, clarified and filled into appropriate sized ampoules sealed by fusion of the glass.
• the injection is sterilised by heating in an autoclave using one of the acceptable cycles.
• the solution may be sterilised by filtration and filled into sterile ampoules under aseptic conditions.
• the solution may be packed under an inert atmosphere of nitrogen.

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• 1993
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  • 1993-03-26 EP EP99201608A patent/EP0972773A1/fr not_active Withdrawn
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  • 1993-03-26 EP EP93908861A patent/EP0640081B1/fr not_active Expired - Lifetime
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• 1994
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• 2000
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