EP0972188A1 - Perfectionnements aux systemes d'electrophorese multicapillaires - Google Patents
Perfectionnements aux systemes d'electrophorese multicapillairesInfo
- Publication number
- EP0972188A1 EP0972188A1 EP99901659A EP99901659A EP0972188A1 EP 0972188 A1 EP0972188 A1 EP 0972188A1 EP 99901659 A EP99901659 A EP 99901659A EP 99901659 A EP99901659 A EP 99901659A EP 0972188 A1 EP0972188 A1 EP 0972188A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- capillaries
- detection means
- detection
- capillary
- light
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/11—Automated chemical analysis
Definitions
- the present invention relates to multicapillary electrophoresis systems
- Electrophoresis techniques are also known using for capillary migration filled with gel or other matrix of separation having the advantage of being particularly handy, easy to charge and of allowing substantially automatic operation, with higher separation speeds than in gel plate electrophoresis thanks to a large applicable electric field
- Multicapillary electrophoresis systems are known in particular in which the laser beam of excitation of the molecules is sent thereon through the walls of the capillaries, along an axis in the plane of the bar, perpendicular to the direction in which the capillaries extend, the fluorescence of the molecules being observed by receiving means having an optical axis perpendicular to the plane of the capillary strip
- the capillaries are held in relation to one another in a glass bowl in which said capillaries extend.
- the molecules which pass through the capillaries are excited after having left said capillaries by laser radiation. which is sent, just at the outlet of the bar, in the plane of said bar and perpendicular to the direction in which the capillaries extend
- the fluorescence of the molecules excited by this radiation is detected by means, for example, of a CCD camera which is oriented with an axis perpendicular to the plane of the capillary strip or with an axis parallel to the capillaries.
- a CCD camera which is oriented with an axis perpendicular to the plane of the capillary strip or with an axis parallel to the capillaries.
- such a system requires the provision of means such as laminar flows or guiding elements preventing the molecules leaving the different capillaries from diverging too far.
- the cuvette requires high precision mechanical production in glass.
- the device should make it possible to ensure a very regular flow and avoid any gas bubble or dust coming to disturb the flow.
- this technique requires the use of different materials - at least as regards viscosity - for the capillaries and the cuvette which have distinct functions, one serving for the separation of molecules, the other for channeling. flows The use of large volumes of solutions to achieve the flow then becomes necessary
- An object of the invention is therefore to propose a multicapillary electrophoresis system which, for chemical and pharmaceutical applications, is robust, inexpensive, reliable, easy to use, and has performance enabling high throughput sequencing and genotyping
- the invention provides a multicapillary electrophoresis system comprising a plurality of juxtaposed capillaries, at least one source for the emission of 'a beam intended to excite molecules lying in its path and inside the capillaries, means for detecting the fluorescence of the molecules excited by said beam
- the invention proposes to arrange the detection means so as to carry out a detection.
- the excitation beam is of elongated section and simultaneously attacks several superposed capillaries
- the space between the capillaries is filled, at least on the path of the excitation beam, with a material whose refractive index is chosen so that the excitation beam does not diverge after having passed through a capillary,
- - it includes means for applying a pressure in the detection cuvette which makes it possible to fill the capillaries with the separation matrix, - it comprises dispersion means for spatially separating the different wavelengths of fluorescence,
- the detection means collect a complete image of the light leaving the capillaries
- the detection means comprise detection means of the charge transfer type (CCD), as well as focusing means,
- FIG. 1 is a schematic perspective representation of a system according to a possible embodiment of the invention
- FIG. 2 is a schematic representation illustrating the arrangement of the detection means with respect to the capillaries for the system of FIG. 1,
- FIGS. 3a and 3b illustrate the use of an excitation beam of circular cross section
- FIGS. 4a and 4b illustrate the use of a beam of elliptical cross section
- FIG. 5 illustrates the use of a matrix of capillaries
- FIGS. 6a and 6b illustrate two possible variants for mounting the capillaries
- FIGS. 7a and 7b are two photographs illustrating the distribution of the excitation beam after having crossed capillaries, according to the index of the medium which surrounds the capillaries and which is crossed by said excitation beam,
- FIG. 8 is a graph on which the response of the system has been plotted as a function of a fluoresceem concentration
- FIG. 9 is a graph on which the signal / noise ratio by capillary has been plotted
- - Figure 10 is a graph on which the signal / noise ratio has been plotted as a function of the laser power, for three different fluorescence concentrations
- - Figure 11 is a graph on which the intensity of the signal ratio has been plotted / noise depending on the aperture of the lens
- FIG. 12a is a graph on which the intensity collected as a function of time has been plotted for an example of sample separation implemented with the system illustrated in FIG. 1,
- FIG. 12b is a graph on which we have plotted as a function of time the intensity collected for an example of DNA separation implemented with the system illustrated in Figure 1
- the multicapillary electrophoresis system shown in FIG. 1 comprises, on an optical table 1
- the CCD camera 6 observes the fluorescence of the molecules excited inside the capillaries by the laser beam F along an optical axis which is parallel to the axis of the capillaries C
- the CCD camera 6 collects the fluorescent light coming directly from the excited molecules, which light forms a cone around the axis of said capillary according to the solid angle between the position of the excited molecules in the capillary and the opening of said capillary
- the resolution of the camera is sufficient, this makes it possible to distinguish the light which emerges from the interior of the capillaries C from that coming from the walls of these and / or from the medium which surrounds them. This results in a considerable improvement in the signal-to-noise ratio.
- capillaries C with an internal diameter of 100 ⁇ m and an external diameter of 300 ⁇ m
- a CCD camera 6 allowing, in combination with the optical means 5, a resolution of the order of 20 ⁇ m
- a black mask forming a diaphragm is advantageously mounted at the outlet of the capillaries C.
- matrix must be understood generally and designates any set of capillaries C in which these are distributed by being superposed on each other in two directions. This term consequently therefore includes just as well matrices made up of several superimposed bars as other capillary arrangements and in particular for example assemblies in which the capillaries are distributed in staggered rows.
- the number of capillaries per matrix can be very variable. Tests were carried out on matrices of 16, 50 and 100 capillaries. A higher number of capillaries per matrix could also be envisaged.
- the excitation beam F emitted by the laser source 7 is sent to the detection cell 4, to attack the capillaries C perpendicular to the direction in which they extend.
- the excitation beam F can then be either of circular section (FIGS. 3a and 3b), in which case it is sent in the plane of a bar of capillaries C to pass successively through the latter.
- it can be elongated (for example elliptical) and attack a strip in an optical direction perpendicular to the plane of said strip, which allows the same beam F to simultaneously attack the different superposed capillaries C (FIGS. 4a and 4b) .
- this allows greater tolerance on the relative position of the capillaries.
- an elliptical beam F will advantageously be used in the case where the capillaries C are distributed not according to a strip, but according to a matrix.
- the capillaries C can be held together by gluing and / or by pre-forms. Furthermore, as illustrated in FIG. 6a, it can be provided on the path of the excitation beam, in the interstices between the capillaries.
- C a material whose refractive index is chosen so that the excitation beam does not diverge after having been crossed by a capillary, in particular a material whose index is lower than that of the capillaries
- This material is chosen to be as transparent as possible and not fluorescent
- FIGS. 7a and 7b The focusing effect obtained with such a material is illustrated by the photographs shown in FIGS. 7a and 7b. It can be seen in these photographs that a beam F illuminating several capillaries C in parallel creates zones of shadow after passing through the capillaries C when the indices of the capillaries C and of the surrounding medium are close, but that the transmitted light is focused when the external index is lower than that of the capillaries C Thus, the light at the outlet of each of the capillaries is focused on the capillary from the next row, which is directly opposite
- This multiple focusing makes it possible, for example, to use the same elliptical beam F to illuminate different rows of capillaries C
- This focusing effect also allows the advantageous use of the laser beam. Almost all of its intensity is focused in the various capillaries.
- Such focusing is achievable with a network of capillaries or even more perfectly by micro lenses. It decreases the laser power required by at least a factor of 3, at the same time creating less noise.
- the material which performs the focusing function may possibly consist of the material which is used for fixing the capillaries.
- solutions are preferred in which a different material is used to prevent the excitation beam passing through the capillaries from diverging. of the one who fixes the capillaries.
- the material which prevents the divergence of the excitation beam can be constituted by the buffer solution in which the capillaries bathe
- FIG. 1 which has been used by the inventors.
- the electrode in box 2 is supplied by a voltage generator sold by the company SPELLMAN
- the inputs and outputs of the capillaries C are electrically connected by means of a buffer or of a polymer solution to the cathode and to the anode of this generator.
- the voltage applied to the cathode can go up to 30 kV for a length of capillaries C between 15 and 60cm
- the detection cell 4 where the capillaries C emerge is a rectangular parallelepiped with opaque walls provided with two lateral quartz windows for the entry and exit of the laser beam F, while another window, also made of quartz, is located in the axis of the capillaries C to allow the collection of the fluorescence light by the optics 5 and the camera 6
- This last window can be replaced by a filter to discriminate fluorescent light from laser light.
- this filter can be placed at the outlet of said window.
- a fourth window on the upper wall of the cell makes it possible to observe with a microscope the alignment of the laser beam F with respect to the capillaries C.
- the adhesive which is used to fix the capillaries C in the detection cell is a transparent adhesive which polymerizes to UV
- Optic 5 is an objective which has a focal length of 1, 2 It is advantageously supplemented by two lenses with a total of 6 diopters, to obtain an enlargement close to 1
- optic 5 can consist of two objectives, the first of which is reversed Between the two objectives, a multi-color dispersion system is advantageously mounted
- the optics 5 can advantageously integrate a network of optical fibers interposed between the outputs of the capillaries and the CCD camera
- the CCD 6 camera is of the type sold by PRINCETON under the name "frame transfer". It allows successive acquisitions to be made without dead time and without a mechanical shutter.
- the active surface of the camera is 6 to 8 mm 2 with a pixel size of 22 ⁇ m / 22 ⁇ m
- the camera is cooled by the Peltier effect to approximately -40 ° C
- the laser is an argon laser (ILT) having a maximum power of around 100 mW at a wavelength of 488 nm.
- ILT argon laser
- a holographic prism makes it possible to eliminate any wavelength other than this wavelength of 488 nm.
- the separation matrix (gel or other) is injected into the capillaries by means of a pump which makes it possible to apply a pressure in the detection cuvette
- Figure 8 gives, as a function of the concentration of fluoresceesulfate injected, the number of charges collected on 25 pixels (summation by software). There is good linearity in the region shown which has been verified between 0 05 and 100 nmol / l
- the two straight lines correspond to the charges collected for a central capillary and one at the edge of the row, respectively The difference is explained by the Gaussian distribution of the laser beam (conventionally elliptical)
- FIG. 9 gives, as a function of the capillary number, the signal / noise ratio (S / N) 1 1
- the tests implemented by the inventors have shown that the sensitivity of the system also depends on the position of the point of impact of the laser relative to the outlet of the capillaries C However, this does not vary much when we do vary the distance between said point of impact and the outlet from 2 mm to 250 ⁇ m, which confirms that most of the light exiting through the opening of the capillaries C is actually collected. To further increase sensitivity, it would then be necessary to s '' approach considerably with the beam F of the exit of the capillaries C Such a gain would involve a weak colhmation of the light, which would partially degrade the resolution of the image
- the inventors made a migration test with the double-stranded sample ( ⁇ X174 from Gibco BRL) in a polymer solution (HPC 0.5%).
- the marker used was the interlayer SYBR (I) (Molecular Probes) The result has been plotted on the graph of FIG.
- the fluorescence light beams at the outlet of the capillaries C - which are collimated - can be directly transmitted to one or more intermediate prisms or to a network of diffraction, to spatially separate the different wavelengths emitted and send them over a network of photodiodes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
Claims
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9801091 | 1998-01-30 | ||
FR9801091A FR2774472B1 (fr) | 1998-01-30 | 1998-01-30 | Perfectionnements aux systemes d'electrophorese multicapillaire |
PCT/FR1999/000184 WO1999039192A1 (fr) | 1998-01-30 | 1999-01-29 | Perfectionnements aux systemes d'electrophorese multicapillaires |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0972188A1 true EP0972188A1 (fr) | 2000-01-19 |
Family
ID=9522409
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP99901659A Withdrawn EP0972188A1 (fr) | 1998-01-30 | 1999-01-29 | Perfectionnements aux systemes d'electrophorese multicapillaires |
Country Status (7)
Country | Link |
---|---|
US (2) | US6613212B1 (fr) |
EP (1) | EP0972188A1 (fr) |
JP (1) | JP2001519913A (fr) |
AU (1) | AU2168799A (fr) |
CA (1) | CA2284901A1 (fr) |
FR (1) | FR2774472B1 (fr) |
WO (1) | WO1999039192A1 (fr) |
Families Citing this family (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2774472B1 (fr) * | 1998-01-30 | 2000-04-21 | Centre Nat Rech Scient | Perfectionnements aux systemes d'electrophorese multicapillaire |
SE9802558D0 (sv) | 1998-07-16 | 1998-07-16 | Hanning Instr Ab | Device for detection of fluorescent |
US6406604B1 (en) * | 1999-11-08 | 2002-06-18 | Norberto A. Guzman | Multi-dimensional electrophoresis apparatus |
US7329388B2 (en) * | 1999-11-08 | 2008-02-12 | Princeton Biochemicals, Inc. | Electrophoresis apparatus having staggered passage configuration |
FR2808089B1 (fr) * | 2000-04-25 | 2002-07-05 | Sebia Sa | Dispositif perfectionne d'analyse d'echantillons par electrophorese multicapillaire a thermo-regulation solide/solide |
JP3893849B2 (ja) * | 2000-05-15 | 2007-03-14 | 株式会社日立製作所 | キャピラリアレイ電気泳動装置及び電気泳動方法 |
US6942773B1 (en) | 2001-01-26 | 2005-09-13 | The Regents Of The University Of California | Particle sizer and DNA sequencer |
FR2827957B1 (fr) * | 2001-07-25 | 2003-09-26 | Picometrics | Appareil de separation par electrophorese sur veine liquide et de detection par fluorescence induite par laser |
US7189361B2 (en) * | 2001-12-19 | 2007-03-13 | 3M Innovative Properties Company | Analytical device with lightguide Illumination of capillary and microgrooves arrays |
EP1682883A1 (fr) * | 2003-11-05 | 2006-07-26 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire |
US8007725B2 (en) * | 2003-11-07 | 2011-08-30 | Princeton Biochemicals, Inc. | Electrophoresis apparatus having valve system |
WO2005047882A2 (fr) * | 2003-11-07 | 2005-05-26 | Princeton Biochemicals, Inc. | Appareil d'electrophorese multidimensionnelle |
JP4045253B2 (ja) * | 2004-03-25 | 2008-02-13 | 株式会社日立ハイテクノロジーズ | キャピラリー及び電気泳動装置 |
WO2006086659A2 (fr) * | 2005-02-10 | 2006-08-17 | Massachusetts Institute Of Technology | Detection electrochimique de fluorescence pour electrophorese capillaire |
CN102879361B (zh) * | 2011-07-12 | 2016-06-08 | 上海理工大学 | 毛细管电泳成像检测系统 |
DE112012004100T5 (de) | 2011-09-30 | 2014-07-10 | Los Alamos National Security, Llc | Programmierbare Voll-Frame-Hyperspektral-Abbildungsvorrichtung |
CN115389599A (zh) * | 2014-03-07 | 2022-11-25 | 生命技术公司 | 用于毛细管电泳的光学系统 |
Family Cites Families (23)
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JPH0392756A (ja) * | 1989-09-06 | 1991-04-17 | Hitachi Ltd | Dna塩基配列決定装置 |
US5194915A (en) * | 1990-09-20 | 1993-03-16 | Millipore Corporation | Photometric apparatus and process |
US5516409A (en) | 1991-02-28 | 1996-05-14 | Hitachi, Ltd. | DNA detector and DNA detection method |
US5529679A (en) * | 1992-02-28 | 1996-06-25 | Hitachi, Ltd. | DNA detector and DNA detection method |
US5290587A (en) * | 1992-07-14 | 1994-03-01 | Hewlett-Packard Company | Method of making an electrophoretic capillary tube |
US5240585A (en) * | 1992-07-14 | 1993-08-31 | Hewlett-Packard Company | Conductive bridge for external control of electroosmotic flow |
US5324401A (en) * | 1993-02-05 | 1994-06-28 | Iowa State University Research Foundation, Inc. | Multiplexed fluorescence detector system for capillary electrophoresis |
JP3563140B2 (ja) * | 1995-01-19 | 2004-09-08 | 株式会社日立製作所 | キャピラリーアレイ電気泳動装置 |
US5439578A (en) * | 1993-06-03 | 1995-08-08 | The Governors Of The University Of Alberta | Multiple capillary biochemical analyzer |
JP3034770B2 (ja) * | 1994-09-29 | 2000-04-17 | 日立ソフトウエアエンジニアリング株式会社 | キャピラリ型電気泳動装置 |
US5675155A (en) * | 1995-04-26 | 1997-10-07 | Beckman Instruments, Inc. | Multicapillary fluorescent detection system |
US5582705A (en) * | 1995-05-19 | 1996-12-10 | Iowa State University Research Foundation, Inc. | Multiplexed capillary electrophoresis system |
US5833827A (en) * | 1995-09-29 | 1998-11-10 | Hitachi, Ltd. | Capillary array electrophoresis system |
US5650846A (en) * | 1995-11-21 | 1997-07-22 | Hewlett-Packard Company | Microcolumnar analytical system with optical fiber sensor |
US5567294A (en) * | 1996-01-30 | 1996-10-22 | Board Of Governors, University Of Alberta | Multiple capillary biochemical analyzer with barrier member |
JP3559648B2 (ja) | 1996-04-23 | 2004-09-02 | 株式会社日立製作所 | キャピラリーアレー電気泳動装置 |
JPH1019846A (ja) * | 1996-06-27 | 1998-01-23 | Shimadzu Corp | マルチキャピラリー電気泳動装置 |
JP3400650B2 (ja) | 1996-06-28 | 2003-04-28 | 株式会社日立製作所 | 電気泳動分離検出方法及び装置 |
US6063251A (en) * | 1997-05-30 | 2000-05-16 | Spectrumedix Corporation | Electrically insulated capillary arrays for electrophoretic applications |
JPH10227740A (ja) | 1997-02-18 | 1998-08-25 | Hitachi Ltd | 多色蛍光検出電気泳動分析装置 |
US6054032A (en) * | 1998-01-27 | 2000-04-25 | 3M Innovative Properties Company | Capillary electrophoresis array |
FR2774472B1 (fr) * | 1998-01-30 | 2000-04-21 | Centre Nat Rech Scient | Perfectionnements aux systemes d'electrophorese multicapillaire |
US6103083A (en) * | 1998-02-20 | 2000-08-15 | Tetragen | Capillary electrophoresis apparatus and method |
-
1998
- 1998-01-30 FR FR9801091A patent/FR2774472B1/fr not_active Expired - Fee Related
-
1999
- 1999-01-29 US US09/402,219 patent/US6613212B1/en not_active Expired - Fee Related
- 1999-01-29 AU AU21687/99A patent/AU2168799A/en not_active Abandoned
- 1999-01-29 EP EP99901659A patent/EP0972188A1/fr not_active Withdrawn
- 1999-01-29 JP JP53899999A patent/JP2001519913A/ja not_active Ceased
- 1999-01-29 CA CA002284901A patent/CA2284901A1/fr not_active Abandoned
- 1999-01-29 WO PCT/FR1999/000184 patent/WO1999039192A1/fr not_active Application Discontinuation
-
2000
- 2000-08-04 US US09/632,573 patent/US7018519B1/en not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
See references of WO9939192A1 * |
Also Published As
Publication number | Publication date |
---|---|
CA2284901A1 (fr) | 1999-08-05 |
US7018519B1 (en) | 2006-03-28 |
FR2774472A1 (fr) | 1999-08-06 |
AU2168799A (en) | 1999-08-16 |
WO1999039192A1 (fr) | 1999-08-05 |
JP2001519913A (ja) | 2001-10-23 |
US6613212B1 (en) | 2003-09-02 |
FR2774472B1 (fr) | 2000-04-21 |
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