EP1682883A1 - Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire - Google Patents

Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire

Info

Publication number
EP1682883A1
EP1682883A1 EP04791057A EP04791057A EP1682883A1 EP 1682883 A1 EP1682883 A1 EP 1682883A1 EP 04791057 A EP04791057 A EP 04791057A EP 04791057 A EP04791057 A EP 04791057A EP 1682883 A1 EP1682883 A1 EP 1682883A1
Authority
EP
European Patent Office
Prior art keywords
sample
capillary
excitation light
sample chamber
along
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04791057A
Other languages
German (de)
English (en)
Inventor
Lajos Ny Rsik
Wilfried Nietfeld
Hans Lehrach
Tarso Benigno Ledur Kist
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority to EP04791057A priority Critical patent/EP1682883A1/fr
Publication of EP1682883A1 publication Critical patent/EP1682883A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6423—Spectral mapping, video display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels

Definitions

  • the present invention relates to methods for measuring fluorescence of at least one sample in at least one sample chamber, in particular to methods for measuring fluorescence of samples in capillaries of a substance separation device like e.g. an isoelectric focusing device. Furthermore, the present inventions relates to fluorescence measuring devices and substance separation devices like e.g. isoelectric focusing devices, particularly being adapted for conducting the above methods .
  • Isoelectric focusing is used for separating molecules (e.g. proteins) with a net charge depending on the surrounding pH value.
  • the basic of isoelectric focusing devices is the formation of pH gradients along a gel lane or a capillary filled with gel or polymer solution. Methods to generate this pH gradient are generally known.
  • "Carrier Ampholites" made of bifunctional amphoteric (acid and basic ends) buffers molecules are used which separate out to form a smooth pH gradient in applied electric fields within a matrix (e.g. polyacrilamide, agarose, dextran) .
  • a molecule of a sample to be investigated will migrate within the pH gradient toward the anode or cathode until it arrives at a point in pH gradient equal to it's pi value.
  • the pH gradient is generated automatically simple by applying the electrophoresis electric field (see D. F. GarfrLn et al. "Isoelectric Focusing Methods” in “Enzymol.” vol. 182, 1990, p. 459-477) .
  • a second commonly known method is the use of the so called "acrylamido buffers for immobilized pH gradients". In this components of the buffer react and are covalently attached to acrylamide derivatives to create immobilized pH gradients (see Isoelectric focusing in immobilized pH gradients: principle, methodology and some applications", Bjellquist B., Ek K. , Righetti P.
  • the focussing points of the sample can be detected with restricted precision only. Furthermore, the conventional fluorescence measurement is adapted for detecting fluorescence in a single capillary only. Samples in multi-channel systems can be analyzed with more complex devices or complex procedures only.
  • a method of measuring fluorescence of at least one sample in at least one sample chamber extending along a longitudinal ref- erence direction comprises the steps of irradiating the sample along an excitation light path and detecting fluorescence from the sample along a detection light path, wherein one of the excitation and detection light paths is directed through the sample chamber parallel to the reference direction and the respective other one of the detection and excitation light paths is directed in a direction deviating from the reference direction.
  • One of the advantages of the present invention is given by extending one of the detection and excitation light paths parallel to the longitudinal extension of the sample chamber. This setup allows an essential reflection loss reduction and an improved measurement geometry.
  • the method of the invention allows simultaneous measurements in a plurality of channels (chambers) providing a high parallelism of measurements, and/or measurements with a plurality of samples in one chamber providing a high ulti- plexation.
  • the ex- citation light path extends about perpendicularly relative to the detection light path. This may have advantages with regard to a further reflection loss and scattering light reduction. Further advantages with regard to an extended information contents and to the ultiplexation may arise, when a spectrally resolved detection of the fluorescence is provided.
  • the detection is associated with a determination of at least one location (position and/or size) of the at least one fluo- rescing sample to be investigated in the sample chamber.
  • an isoelectric focusing device with axial excitation or detec- tion allows the use of multicapillaries and multiplexation.
  • the irradiating and detecting steps are conducted for each of the sample chambers separately, advantages with regard to precision and reproducibility may arise. If the irradiating and detecting steps are conducted for all sample chambers si- multaneously, the measurement speed can be essentially increased.
  • the samples to be investigated are labelled with dif- ferent fluorophores or nanoparticles allowing an improved multiplexation of the measurement.
  • the method of the invention is used for the operation of an isoelectric focusing device with at least one straight sample chamber with axial laser light excitation or detection.
  • the sample chamber is a part of an electropho- retic separation device and a sample positioning step is provided with a migration of the at least one sample through the sample chamber under the influence of an electric field.
  • a pH gradient may be formed in the sample chamber with conventional techniques .
  • the sample chamber comprises a capillary and the excitation light path extends axially along a longitudinal axis of the capillary, while the detection light path extends through a transparent wall portion of the capillary.
  • the excitation light is focussed directly to an end of the capillary allowing a direct in-coupling of excitation light into the sample, sample carrier or matrix material.
  • a position-selective signal of the multi-channel detector may be used directly for a paral- lei determination of the location, size and/or distribution of one ore more samples.
  • the detector device in particular a single-channel detector
  • the detector device can be moved along the capillary for sequentially obtaining the above geometry- related information. This variant may have advantages for special measurement purposes (e.g. high resolution measurements at certain locations) .
  • the sample chamber comprises a capillary and the detection light path extends along a longitudinal axis of the capillary, while the excitation light path extends through a transparent wall (or transparent wall portion) of the capillary.
  • a detector e.g. CCD, PMT, or APD
  • the excitation light source can be moved along the capillary for allowing an evaluation the above geometry-related information from a position of the excitation light source.
  • the at least one sample is irradiated with at least two different wavelengths, different fluorophores can be excited.
  • the at least one sample chamber is placed in contact with a heat dissipating material.
  • a heat dissipating material E.g. the capillaries of an isoelectric focusing device are placed between dielectric plates, i.e., sandwiched between dielectric solid materials, in order to have a more efficient heat dissipation, which in turn allows more elevated voltages to be applied and correspondingly im- proved separation results.
  • a device for measuring fluorescence of at least one sample comprising at least one sample chamber for accommo- dating the at least one sample and extending along a longitudinal reference direction, wherein an excitation light source for irradiating the sample along an excitation light path and a detector device for detecting fluorescence along a detection light path are arranged such that one of the excitation and detection light paths extends through the sample chamber parallel to the reference direction and the respective other one of the detection and excitation light paths extends in another direction.
  • the device for measuring fluorescence comprises a plurality of sample chambers each being adapted for accommodating at least one sample. Preferably, all sample chambers extend in parallel along the same reference direction, so that the adaptation of the excitation light source and the detector to the chamber arrangement can be facilitated.
  • each sample chamber comprises one capillary for accommodating a sample carrier or matrix material containing or carrying the sample to be investigated.
  • the capillary has at least one transparent wall portion.
  • the excitation light source comprises at least one laser. This may have advantages with regard to intensity and wavelength control depending on the particular sample to be investigated. If the excitation light from each laser is sub- mitted to the sample chamber via at least one light guiding fibre, further advantages with regard to the use of a single laser for a plurality of capillaries and a flexible adaptation of the excitation light source to the geometry of the measuring device are obtained.
  • the detector device comprises a multi-channel detector extending along the capillary, parallel provision of position selective information can be facilitated. If the detector device is movable along the capillary, different portions of the capillary can be investigated with different resolution.
  • a separation device in particular for isoelectric focusing of sam- pies is provided comprising a measuring device having in particular the features characterized above.
  • An essential advantage of the invention is given by the pro- vision of isoelectric focusing devices which are capable to use simultaneously multichannels and/or multiplexation. Moreover, they are able to work with multiplexation in a capillary.
  • multichannels multi-capillaries
  • multiplexation many samples per capillary
  • the separation device comprises a first sample chamber holding block for carrying a first end of the at least one sample chamber.
  • the first sample chamber holding block is further adapted for accommodating samples to be introduced into the respective sample chamber.
  • each sample chamber is con- nected with a second sample chamber holding block which is capable of collecting samples from the at least one sample chamber.
  • a second sample chamber holding block which is capable of collecting samples from the at least one sample chamber. If the first or second sample chamber holding block being made of a transparent material, the transmission of excitation or fluorescence light can be facilitated.
  • Figures 1 to 3 schematic representations of various embodiments of the invention
  • Figure 4 a perspective view of a multichannel isoelectric focusing device according to the invention
  • Figure 5 a schematic representation of an embodiment adapted for spectrally resolved fluorescence measurements
  • Figure 6 an illustration of a conventional fluorescence measurement set-up.
  • the invention is described in the following with reference to an isoelectric focusing device with electrophoretic molecule separation in capillaries. It is emphasized that the invention can be implemented in an analogous way with modified isoelectric focusing devices or other applications of fluorescence measurements. As an example, capillaries with other sizes or shapes can be used, or straight open channels or gel lanes can be used instead of the capillaries. Although straight sample chambers are preferred, the invention can be implemented with bend or curved chamber shapes.
  • Figure 1 shows an embodiment of a fluorescence measurement device 100 according to the present invention, comprising a sample chamber 10, an excitation light source 20, a detector device 30, a first holding block 40, and a voltage source 60.
  • the sample chamber 10 is a hollow compartment or channel hav- ing a main extension parallel to the x-direction (longitudinal reference direction) .
  • the sample chamber 10 is a capillary 11, made of a plastic or quartz, e.g. with the following dimensions: length: 10 mm, outer diameter: 200 ⁇ m, inner diameter: 75 ⁇ m.
  • the fluorescence measurement de- vice 100 comprises at least one capillary.
  • the number of capillaries is adapted to a typical format used for parallel sample processing, e.g. in micro- or nanotiter plates, like 8, 16 or higher multiples. In this case, the in- troduction of samples into the capillaries e.g. with conventional multichannel-pipettes is facilitated.
  • a first end 12 of the capillary 11 is fixed to the first holding block 40, while the second end 13 is connected with a second holding block (50, see figure 4). Both ends have an open connection with sample reservoirs in the blocks (see below) .
  • Capillary 11 is filled with a gel or solution 2 serving as a carrier material for a sample 1 migrating through the capillary under the influence of a driving voltage.
  • the carrier material 2 is introduced into the capillary in conventional manner with a pumping device (not shown) .
  • the excitation light source 20 comprises a laser, like e.g. a Ar ion laser or a semiconductor laser (preferred due to small size) being adapted for fluorescence excitation and emitting e.g. in the blue or green wavelength range (e.g. 488 nm, power: 50-150 mW) .
  • Laser 20 may be equipped with a light guiding fibre (not shown) .
  • Excitation light from laser 20 is focused axially and parallel to the x-direction to the second end 13 of the capillary 11. Excitation light travels along an excitation path through the capillary. If the device 100 comprises a plurality of parallel capillaries, a corresponding number of light guiding fibers can be fed from one laser to the ends of the capillaries. The focus at the end 13 of each capillary can be im- proved by a focusing optic (focusing lens, reference numeral
  • the detector device 30 comprises generally a light sensitive fluorescence light detector like known in the art, e.g. a photodiode, an APD or a photomultiplier .
  • Figure 1 schematically shows a CCD line detector 30 extending adjacent to the outer side of the capillary 11.
  • the CCD line detector 30 comprises a plurality of detector elements (pixel) collecting light from fluorescing molecules within the capillary along a detection path.
  • the signals of the detector elements represent the position of a fluorescing molecule in x-direction.
  • the CCD line detector 30 is e.g. the spectroscopic camera DV401 with 1024 x 128 pixels from Andor Technology . (distribu- tion in Germany LOT Oriel civil Europe) .
  • the first holding block 40 is adapted for accommodating samples to be introduced into capillary as described below with reference to figure 4.
  • Block 40 is preferably made of an electrically conducting material, e.g. platin covered inox or plastic with platin electrodes.
  • the voltage source 60 is a high voltage power source as known in the art of electrophoretic separation techniques, which is connected via an electrically conducting connection 61 with the carrier material 2 at the end 13 of the capillary 11.
  • Figure 2 shows an alternative embodiment of the measuring device 100 being similar like the embodiment of figure 1.
  • the only difference concerns the detector device 30 which is a movable single- or multi-channel detector.
  • the x-position of the detector can be changed with a driving device (not shown) .
  • Control signals of the driving device or the position of the detector 30 represent the position of a fluorescing molecule in x-direction.
  • a single-channel detector a PMT or APD can be used.
  • a multi-channel detector a CCD camera can be used.
  • the excitation source e.g. laser 20 is arranged for illuminating the samples within the capil- lary according to the y-direction.
  • Laser 20 is movable in x- direction with a driving device (not shown) .
  • Control signals of the driving device or the position of laser 20 represent the position of a fluorescing molecule in x-direction.
  • the detector device 30 is arranged at the end 13 of the capillary 11.
  • Figure 4 illustrates a multi-channel arrangement with a plurality of capillaries 11 between the first and second holding blocks 40, 50.
  • the first holding block 40 contains conic holes 41 for accommodating samples to be introduced into the respective capillaries.
  • the first holding block 40 is made by a metal and works as the ground electrode or, alternatively, it may be made by any dielectric material and in this case electrodes must be inserted in order to keep this end at the right electrostatic potential.
  • the second holding block 50 is made of transparent material like plastic, e.g. PMMA, or quartz. It contains a central channel 51 for collecting carrier material from the capillaries.
  • the excitation light source 20 is movable along the second holding block 50, i. e. parallel to a z-reference direction.
  • the laser 20 scans all capillaries, once at a time.
  • the illumination is done in the axial x-direction, allowing a lot of light to be used to excite all bands at once.
  • the detector 30 is a movable CCD- camera as shown in figure 2.
  • the fluorescent light might be detected by a PMT or PDA.
  • the measuring device may comprise a heat dissipating compo- nent 70 (schematically shown) placed in contact with the at least one or all capillaries 11.
  • the heat dissipating component 70 can be formed as a monolithic plastics block into which all capillaries are molded.
  • Reference numeral 80 indicates a control device being adapted for controlling the function of the components of the measuring device 100.
  • the control device 80 contains a circuit for determining at least one location, size or distribution of the at least one sample along the longitudinal extension of the sample chamber (location determining device) .
  • samples are introduced into the conic holes 41 in the first sample holding block 40.
  • a high voltage is applied to the carrier material (buffer) that fills the hole of the block that holds the opposite end of the capillaries or channels 11.
  • the application of this high voltage will make the molecules to migrate into the channels or capillaries 11.
  • An alternative way to introduce the samples is simple applying a vacuum to the hole on the exit end 13 of the capillaries. Afterwards, the sample left over in the conic holder is removed and one of the IEF running buffers is placed in.
  • the detection systems are activated in order to get the spatial profile of the sample bands (e.g. reference numeral 1 in figures 1 and 2) .
  • the detection starts with the movement of the laser as shown in figure 4.
  • the laser light propagates along the capillary by internal total reflection causing the excitation of the labeled or unlabeled bands that are focused around their isoelectric points.
  • a CDD camera 30 takes a picture of the whole capillary or only pieces of them. These photos are then later mounted to get a panoramic photo.
  • a transmission diffraction grating 31 is positioned in the detection light path, as shown in figure 5. Between the diffraction grating 31, optical lenses 32 and a mirror 33 are arranged before the CCD camera 30.
  • An alternative to the diffraction grating is an optical filter transmitting fluorescence light only.
  • fluorophores e.g. FITC, NDA, succini idyl ester, nanodots
  • samples are labeled with different nanodots, that allow the simultaneous analysis of multiple samples in a single channel (multiplexation with quantum nanodots) .
  • Nanodots are described e.g. in "High quantum yield blue emission form water-soluble Au nanodots" Zheng J. , Petty J. T., Dickson R. M. in "J. Am. Chem. Soc.” 2003, 125(26), p. 7780- 7781.
  • CCD camera detection is the use of a pho- tomultiplier tube (PMT) or an avalanche photodiode (APD) .
  • PMT pho- tomultiplier tube
  • APD avalanche photodiode
  • the whole capillary must be scanned, as most of these devices don't have spatial resolution.
  • the spectrum is also scanned at each position in order to carry out multiplexed experiments.
  • PMT pho- tomultiplier tube
  • APD avalanche photodiode
  • APD a moving transmission diffraction grating
  • This oscillatory movement would be synchronized with the rate of data acquisition, so that spatial position and spectral interval of the detected photons are precisely known.

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

L'invention concerne un procédé pour mesurer la fluorescence d'au moins un échantillon (1) dans au moins une chambre d'échantillon (10, 11) s'étendant dans le sens longitudinal de référence (x), comprenant les étapes de positionnement de l'échantillon (1) dans une chambre d'échantillons (10, 11), l'irradiation de l'échantillon (1) par un éclairage d'excitation provenant d'une source d'éclairage d'excitation (20), le long d'un chemin d'excitation, et la détection de l'éclairage de fluorescence de l'échantillon (1) au moyen d'un dispositif de détection (30) le long d'un chemin d'éclairage de détection, un des passages d'éclairage d'excitation et de détection s'étendant à travers la chambre d'échantillons (10, 11) parallèlement au sens de référence longitudinal et l'autre passages d'éclairage d'excitation et de détection s'étendant dans un sens (y) déviant du sens (x) de référence longitudinal. L'invention concerne, de plus, un dispositif pour mesurer la fluorescence selon le procédé de l'invention.
EP04791057A 2003-11-05 2004-10-29 Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire Withdrawn EP1682883A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04791057A EP1682883A1 (fr) 2003-11-05 2004-10-29 Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP03025455 2003-11-05
PCT/EP2004/012300 WO2005050187A1 (fr) 2003-11-05 2004-10-29 Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire
EP04791057A EP1682883A1 (fr) 2003-11-05 2004-10-29 Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire

Publications (1)

Publication Number Publication Date
EP1682883A1 true EP1682883A1 (fr) 2006-07-26

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EP04791057A Withdrawn EP1682883A1 (fr) 2003-11-05 2004-10-29 Procedes et dispositifs pour mesurer la fluorescence dans un echantillon dans un capillaire

Country Status (3)

Country Link
EP (1) EP1682883A1 (fr)
BR (1) BRPI0416120A (fr)
WO (1) WO2005050187A1 (fr)

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
US5104512A (en) * 1990-05-14 1992-04-14 Labintelligence, Inc. Gel electrophoresis system
US5184192A (en) * 1991-07-17 1993-02-02 Millipore Corporation Photometric apparatus with a flow cell coated with an amorphous fluoropolymer
US5784154A (en) * 1992-01-13 1998-07-21 Anthony R. Torres Electrophoresis separation in a capillary passage
JPH10239278A (ja) * 1997-02-24 1998-09-11 Hitachi Ltd 電気泳動装置
FR2774472B1 (fr) * 1998-01-30 2000-04-21 Centre Nat Rech Scient Perfectionnements aux systemes d'electrophorese multicapillaire
DE60220497T2 (de) * 2001-01-26 2008-01-31 Biocal Technology, Inc., Irvine Optische detektion in einem mehrkanaligen bioseparationssystem
FR2827958B1 (fr) * 2001-07-25 2003-09-26 Picometrics Dispositif d'analyse par fluorescence induite par laser et appareil de separation avec un tel dispositif

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2005050187A1 *

Also Published As

Publication number Publication date
WO2005050187A1 (fr) 2005-06-02
BRPI0416120A (pt) 2007-01-02

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