EP0861431B1 - System zur blutanalyse, mit mitteln zur aufbewahrung und zum transport des blutes, sowie zur selbsttätigen herstellung von blutauftragsproben - Google Patents

System zur blutanalyse, mit mitteln zur aufbewahrung und zum transport des blutes, sowie zur selbsttätigen herstellung von blutauftragsproben Download PDF

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Publication number
EP0861431B1
EP0861431B1 EP96937023A EP96937023A EP0861431B1 EP 0861431 B1 EP0861431 B1 EP 0861431B1 EP 96937023 A EP96937023 A EP 96937023A EP 96937023 A EP96937023 A EP 96937023A EP 0861431 B1 EP0861431 B1 EP 0861431B1
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EP
European Patent Office
Prior art keywords
blood
specimen
sample
slide
smear
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Expired - Lifetime
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EP96937023A
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English (en)
French (fr)
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EP0861431A1 (de
Inventor
Cynthia J. Sperber
Daniel Dashui Gao
Marshall D. Graham
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Beckman Coulter Inc
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Coulter International Corp
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G01N2035/00544—Mixing by a special element, e.g. stirrer using fluid flow
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/112499—Automated chemical analysis with sample on test slide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/113332—Automated chemical analysis with conveyance of sample along a test line in a container or rack
    • Y10T436/114998—Automated chemical analysis with conveyance of sample along a test line in a container or rack with treatment or replacement of aspirator element [e.g., cleaning, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y10T436/117497—Automated chemical analysis with a continuously flowing sample or carrier stream
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Definitions

  • the present invention relates to blood analyzing systems of the kind that detect and analyze the composition of whole blood samples. More particularly, it relates to an improved blood analyzing system that is capable of automatically storing and transporting blood specimens and, upon pre-programmed and/or operator-determined command, of providing a blood smear on a microscope slide, e.g. for further study, verification and/or analysis.
  • the COULTER® STKS Blood Analyzer utilizes a unique hematology system in which three independent energy probes (viz. direct current, radio frequency and a stable helium-neon laser) cooperate to measure a blood cell's volume, conductivity and light scatter.
  • Memory and control systems of the analyzer store and process detected cell information to provide the user with data (e.g. screen displays or print-outs) indicating the relative proportions of different cell types/populations constituting respective blood samples.
  • data e.g. screen displays or print-outs
  • the analyzer can provide a wide variety of blood information, including hemograms, five part differential white blood cell counts and reticulocyte enumeration and analysis. While such information is useful in itself to diagnosticians, it sometimes occurs that a blood smear slide would be useful for further microscopically analyzing the sample and/or verifying the results of the analyzer system.
  • U.S. Patent No. 5,209,903 describes a system for automating these steps. It includes a conveyor assembly for moving racks of blood vials, first past one or more separate blood analyzers, and then past an automated slide-making apparatus. Each of these separate apparatus includes its own blood aspiration assembly, as well as the necessary supply, disposal and cleaning devices and fluids that are required to handle successive blood specimens in a clinical manner.
  • each separate apparatus comprises its own bar code reading system to identify the individual vials, and its own vial handling mechanisms to present vials from the conveyor to its bar code reader and aspirator.
  • the analyzer and slidemaker apparatus will often be arranged in different configurations, so the conveyor system for transporting vial racks are frequently custom designed or individually modified for each installation, which can present significant costs.
  • DE-A-3503475 discloses a blood-analyzing system which comprises the combination of a blood analyzer and a slide-maker.
  • a blood sample is drawn by a single aspirating needle, and one is presented to the analyzer while the other is presented to the slide-maker.
  • the other is used to prepare a blood smear.
  • One blood smear is made for every sample analysed, with the analyzer and slide-marker operating in parallel.
  • EP-A-0517477 discloses a blood-analyzing system in which a second analysis of a blood sample is made after a first analysis indicates a need for further analysis.
  • One significant purpose of the present invention is to provide for automated blood analysis systems such as described above, new capability for automatically producing blood smear slides of their tested samples, in a manner providing sample integrity, homogeneity and identification.
  • an automated blood analysis system comprises:
  • a method for selectively producing a blood smear in which a blood sample is aspirated from a container and analysed to detect certain blood characteristics, and a blood smear of said sample is produced on a microscope slide comprises the steps of:
  • the systems includes two mainframe device, viz, an automated blood analyzer 20 and an automated slidemaker 40.
  • Devices 20 and 40 are physically coupled together by a specimen transmission assembly 60; they are electronically coupled to a slide production control module 10.
  • the blood analyzer 20 preferably comprises the COULTER® STKS Blood Analyzer available from Coulter Corporation of Miami, Florida.
  • Such an analyzer includes means for receiving a plurality of blood sample containers, indicated by blood sample input-store 21 in FIG. 1, and shown schematically in FIG. 2 to include input and output bins 37, 38 and a conveyor system 39.
  • Each of the bins is adapted to receive a plurality of racks 30 of blood sample vials 31.
  • the vial racks are fed onto the conveyor system 39 which moves the racks 30 to an aspiration station, aligned with the aspirator needle 16 contained within a bellows assembly 17 adapted to facilitate needle cleansing.
  • a vial extraction assembly 18 moves the rack vials sequentially into punctured, aspirating relation with aspirator needle.
  • the conveyor assembly 39 is periodically tilted about its longitudinal axis to provide mixing of the blood in vials conveyed thereby. After each vial of a rack has been aspirated in sequence, the rack is moved to the output bin 38, and a new rack is moved onto the conveyor from the input bin.
  • the analyzer 20 further includes blood specimen processor subsystem which includes the specimen aspiration means 32 for withdrawing blood specimens to be analyzed from the respective sample container 31 (see FIG. 2), and scan means 22 for scanning such specimens to detect specimen characteristic data, e.g. red blood cell, white blood cell and differential count, as provided by subsystems 22a, 22b, 22c, respectively.
  • the data from the subsystems of processor 22 is stored and analyzed, e.g. by microprocessor subsystem 25, as operated with inputs and controls from system control 24. After analytical computations are completed, results of the data can be printed out or displayed by subsystem 27, e.g. CRT display screen, and are forwarded to slide production control 10.
  • Control 10 can embody an algorithm(s) that will decide if non-normal data conditions exist such as to initiate a slide production operation.
  • the operator interface 28 also can provide access for the user to input instructions, e.g. the desire for a slide to be made from the specimen.
  • the automated slidemaker 40 is shown in more detail in FIG. 3 and in general comprises a drop dispensing subsystem 41, a slide manipulation and smear subsystem 42, and a slide ID mark subsystem 43. As illustrated in FIG. 1, logic and control 44 is coupled to operate these subsystems.
  • FIG. 3 also shows a slide cassette 52 from which slides are extracted and presented to a manipulation assembly 53 (sometimes referred to as a "slide truck"), which in turn grasps and transports the slides to a movable transport platform 54 (sometimes referred to as a "slide shuttle").
  • the transport platform moves slides first to a marking station 43 comprising a label supply 55, a print media supply 57 and printer 56.
  • the platform then moves a labeled slide to the drop dispense station 41 where a smear specimen drop is dispensed. Thereafter, the transport platform moves to a smear station where the slide manipulation assembly moves between the two positions illustrated. In moving between these two positions, a blood drop is smeared with the edge of an angularly oriented, next successive slide from the cassette. After the smear step, the slide is moved onto the belt of a dryer station 58 and then to an outloader system (not shown). The details of the slidemaker apparatus construction and function are described in US-A-5 766 549.
  • FIG. 4 shows a preferred assembly 60 for automatically transmitting specimen blood between separate mainframe apparatus, such as 20 and 40, with minimized sample degradation and improved homogeneity.
  • Assembly 60 comprises a blood conduit 63 having an inlet 61 for successively receiving smear specimen portions respectively from different blood sample containers in the analyzer apparatus 20, and an outlet 62 for delivering such smear specimen portions to the dispensing station 41 of apparatus 40.
  • conduit 63 winds around a central longitudinal axis 61 a to provide a curvilinear flow path that imparts substantial angular velocity components to blood moving therein. Such configuration is referred to herein as "generally helical".
  • conduit means 63 comprises a plastic tube which is wound, generally in the form of a helix, around a support cylinder 64 extending between the mainframe apparatus.
  • One preferred tubing comprises a laboratory silicone type commercially sold by Dow Corning Corporation under the trademark SILASTIC.
  • SILASTIC Standard Industrial Standard
  • a tube of 18 inches linear length and inner diameter of .04 inches is helically wound around a cylinder with length of 10 inches having a 0.25 inch diameter.
  • the resulting helix has about 10 turns and a pitch of about 2 inches.
  • other generally helical configurations will function to provide a curvilinear flow path that imparts substantial angular velocity components to blood transported thereby.
  • FIG. 5 illustrates prior art systems wherein blood is transported with a primarily linear velocity in the direction of a transport conduits longitudinal axis.
  • This linear velocity profile allows cells to settle.
  • the linear or rectilinear velocity gives rise to cells settling quickly because of gravitational forces.
  • Such settling has the effect of altering the homogeneity of the blood as it moves between the blood analyzer 20 and slide maker 40.
  • settling can alter the cell morphology due to the relatively lengthy contact time between the cells and internal wall of the conduit as the cells are moved through the conduit.
  • the helical path provided by the assembly of the present invention provides, as well as “x”, “y” and “z” linear velocity components, continuous radial accelerations to blood transported from inlet to outlet.
  • the blood has a continuous angular velocity R and rectilinear velocities which vary in magnitude and direction along the path shown schematically in FIG. 6.
  • This angular velocity R and changing rectilinear velocities are believed to cause a tumbling movement of blood cells C during transport, which minimizes specimen degradation in comparison to the specimen quality provided by the differential "x" velocity profile incident to straight tubing transport, as illustrated in FIG. 5.
  • y and "z” components provided during the movement of blood around a curvilinear winding such as helix, provides more effective stirring of the blood than is provided by a straight line path, whereby the homogeneity of the blood is better preserved between the time it was aspirated and the time it is used to produce a blood smear.
  • a plurality of blood sample containers e.g. sealed glass vials 31, are inserted into the specimen container receiving system (shown in FIG. 2) of analyzer apparatus 20.
  • the analyzer's aspirator system 32, 35, 33 is actuated to withdraw a sample of blood through one channel 35 (see FIG. 8) of needle 16, to divider valve 14 for processing and analysis by subsystem 22.
  • a blood smear specimen is next aspirated through a second channel 36 (see FIG. 8) of needle 16 and introduced into blood storage and transmission system 60 (see FIG. 4).
  • inlet valve 65 is opened and negative pressure from one of a bank of sources 68 is coupled to outlet end 62 to move the smear specimen portion into the helical path provided by the windings of tube 63.
  • the specimens are from substantially the same location within a given vial 31. Also, because those specimens are taken sequentially from a given vial 31 (before any other vials are sampled), the specimens for analysis by apparatus 20 and for slide smear preparation by apparatus 40 are temporally proximate.
  • the apparatus 20 proceeds to complete analyzation of the first blood specimen for which a corresponding blood smear specimen portion now resides in the transmission assembly.
  • the fluid pressure source of assembly 60 is cycled to produce forward and reverse movement of the blood along the helical conduit 63. This can be accomplished by alternately coupling inlet and outlet regions to negative pressure and vent ports of fluid sources 68.
  • a smear cycle initiation signal is relayed to control 44 of apparatus 40 and to the system control 24 of apparatus 20.
  • specimen identification data is forwarded to logic and control 44 to assure that a properly identified slide is presented to dispense station 41, and other data (e.g. smear speed information) is forwarded to assure that a blood smear is made according to an algorithm which takes into account various blood parameters (e.g. hematocrit).
  • the specimen recycling ends and the smear specimen is forwarded to dispensing station 41, e.g. by opening outlet valve 66 and coupling inlet region 61 to the 5 psi pressure of fluid source bank 68.
  • a metered blood drop is dispensed on the marked slide.
  • a cleaning solution from source bank 68 is washed through the transport tubing to prepare it for receiving its next smear sample portion from the analyzer 20.
  • a drying is effected by injection of 30 psi air from source bank 68 through the conduit.
  • the transmission system 60 forwards the smear specimen to a waste sump in apparatus 40, instead of to the dispensing station 41.
  • an alternative embodiment of the transmission system 70 incorporates a plurality (here two) helical tubes 73a, 73b, which are coupled in parallel between three way inlet valve 71 and three way outlet valve 72 so that one path can be receiving, recycling and outputting a blood sample, while another path is being cleaned.
  • each of helical tubes 73a, 73b has an inlet end 71a, 71b and outlet end 72a, 72b couplable to the bank of fluid sources 78 as shown in FIG. 7, by solenoid valves 91, 92, 93 and 94, respectively.
  • Specific sources within fluid source bank 78 are selectable for the inlet ends 71a, 71b by solenoid valves 81, 82, 83, 84 and 85 and for the outlet ends 72a, 72b by solenoid valves 86, 87, 88 and 89.
  • Branch inlet solenoid valves 75a, 75b are located respectively just upstream of upstream blood detector devices (e.g. photodetectors) 77a, 77b and branch outlet solenoid valves 76a, 76b are located respectively just downstream of downstream blood detectors 79a, 79b.
  • both the FIGS. 4 and 7 fluid systems have controls that operate the valves thereof to provide means for introducing an intervening gas sector at a position after a lead sector of a blood smear specimen portion in the helical tubing.
  • the gas sector is introduced behind about 50 ⁇ l of forward specimen blood.
  • This feature of the present invention allows the forward specimen sector to pick up residual cleaning solution from the tube and to isolate this residue from the trailing specimen sector.
  • the forward or lead specimen sector is directed to the sump 102 of apparatus 40 and the trailing sector is metered by valves 114, 115 for dispensing by station 41.
  • the mode of creating such intermediate gas sector will be clear from the following description of a cycle of operation of the FIGS. 7-9 system.
  • a sequence of operation commences after analyzer 20 has withdrawn its specimen, as described above.
  • a negative pressure is coupled to the aspirator passage side 35 of needle 16 (with needle passage 36 coupled to vent) to withdraw an analyzer blood portion for the analyzer to perform its functions.
  • a smear-specimen portion is withdrawn from the vial 31 by coupling the passage 36 in needle 16 to vacuum, e.g. via solenoid valves 87, 93, 76a, 75a, 71, 112 and 111.
  • Blood is withdrawn from the vial and flows along the vacuum passage until the lead end of the smear-specimen portion reaches blood detector 113, at which time aspiration vacuum is signaled to stop and the needle 16 is withdrawn from vial 31.
  • the withdrawn smear-specimen sample portion continues to be advanced until its lead end is detected by detector 77a, whereupon valve 87 terminates the vacuum.
  • the line to valve 91 is next coupled to atmospheric pressure via vent valve 86, and air is injected into a region behind the leading 50 microliter segment, which is now drawn forward by vacuum via 87.
  • valve 75a With the protective gas region separating the trail end of the lead segment from the remainder of the smear-specimen, the valve 75a is opened, valve 91 is closed, and vacuum 87 draws the remainder of the sample forward until detector 79a detects the lead segment, at which point valves 75a and 76a are both closed.
  • cleaning liquid is introduced to clean upstream of valve 71, and a second specimen can be introduced into reservoir 73b in a similar manner to that just described.
  • the specimen in the reservoir 73a now comprises a 50 microliter lead segment and a 200 microliter trailing segment, separated by a gas sector that positively maintains separation between those blood specimen segments.
  • the segments are then recycled forward and rearward between closed valves 75a, 76a by alternately coupling valves 91 and 93 to vacuum and vent (91 to 81, 82 and 93 to 87,86).
  • the magnitudes of the vacuum are selected to cause a blood speed along the helical tube reservoir to be about 3 to 5 inches per second, which maintains adequate mixing without cell damage.
  • the sample portion is discharged from the helical transport, store and mixing reservoir section 73a by coupling the low (5 psi) positive pressure source 85 to open valve 91 through valve 72.
  • the initial 50 microliters of blood, gas region and a lead portion of the trailing segment are discharged through valve 101 pinch valve, pumps 114, 115 and dispensing nozzle to waste.
  • detector 79a senses the edge between the trailing end of the lead blood sector and the air sector, the pressure from source 85 is stopped and the pinch valves 114, 115 are actuated to meter a blood drop sample (e.g.
  • the 30 psi air source from bank 78 is coupled to flow through that path and purge and dry the path.
  • One preferred cleaning fluid is a balanced isotonic saline solution such as is commercially available from Coulter Corporation of Miami, Florida under the tradename ISOTON®. This solution serves to clean the paths of residual blood from the previous sample and is dried or blown out by the 30 psi air purge of the tubing. The residue in the tubing (after blow out and drying) is picked up by the leading segment of the next sample to use that pathway (which leading segment, it will be recalled, is subsequently discarded to waste, also).
  • the exterior of both needle 16 and dispensing probe 42 are also both cleaned and dried after each specimen operation.
  • the sample reservoir 73b is discharged and metered onto the next successive slide while the reservoir 73a is cleaned and reloaded with the next successive sample. It will be appreciated that this parallel sequence of loading and cleaning the reservoirs 73a and 73b can continue repeatedly to transport blood samples in a manner maintaining homogeneity, without cell damage.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (8)

  1. Automatisiertes Blutanalysesystem, umfassend:
    (a) eine Blutanalyseeinrichtung (20) zur Analyse einer Blutprobe zur Bestimmung der Bestandteile derselben, wobei die Blutanalyseeinrichtung umfaßt (i) Einrichtungen (21) zur Aufnahme eines Blutprobenbehälters, enthaltend eine zu analysierende Blutprobe; (ii) Einrichtungen (32) zum Ansaugen erster und zweiter Anteile der Blutprobe aus dem Blutprobenbehälter; und (iii) Einrichtungen (22) zur Analyse des ersten Blutprobenanteils zur Bestimmung der Bestandteile desselben;
    (b) eine Objektträger-Bereitungseinrichtung (40) für ein automatisches Herstellen von Blutabstrichen auf einzelnen Mikroskop-Objektträgern, wobei die Objektträger-Bereitungseinrichtung eine Bluttropfen-Ausgabeeinrichtung (41), welche wirksam mit den Ansaugeinrichtungen (32) für ein selektives Ausbringen von einzelnen Tropfen des Bluts auf einem Mikroskop-Objektträger verbunden ist; und Abstricheinrichtungen (42) zum Ausstreichen eines auf einem Mikroskop-Objektträger ausgegebenen Bluttropfens beinhaltet, um einen Blutabstrich auf dem Mikroskop-Objektträger auszubilden; und
    (c) Proben-Transporteinrichtungen (60), welche die Blutanalyseeinrichtung und die Objektträger- Bereitungseinrichtung verbinden, wobei die Proben-Transporteinrichtungen Einrichtungen zum Transport des zweiten Blutprobenanteils von den Ansaugeinrichtungen (32) zu der Tropfen-Ausbringvorrichtung (41) umfaßt, wobei die Transporteinrichtungen Leitungseinrichtungen (63), welche einen Fluidströmungsweg zwischen den Ansaugeinrichtungen und der Tropfen-Ausbringvorrichtung definieren, und Steuer- bzw. Regeleinrichtungen (68) umfassen, welche den zweiten Blutprobenanteil zu einem Strömen entlang des Fluidströmungswegs veranlassen, wobei das Analysesystem dadurch gekennzeichnet ist, daß (a) der Fluidströmungsweg geformt ist, um ein Taumeln bzw. Bewegen der Blutzellen, welche der zweite Blutprobenanteil enthält, bei einer Bewegung der Blutzellen entlang des Strömungswegs zu bewirken, und (b) die Steuer- bzw. Regeleinrichtungen arbeiten, um den zweiten Blutprobenanteil zu einer Bewegung nach vorwärts und rückwärts entlang des Strömungswegs veranlassen, wodurch der zweite Blutprobenanteil gespeichert wird, während der erste Blutprobenanteil analysiert wird, und die Homogenität und die Zellmerkmale des zweiten Blutprobenanteils erhalten werden.
  2. System nach Anspruch 1, worin die Ansaugeinrichtungen (32) eine Ansaugnadel (16) für ein Ansaugen des zweiten Blutprobenanteils aus im wesentlichen derselben Position innerhalb des Probenbehälters wie für den ersten Probenanteil beinhalten.
  3. System nach Anspruch 1 oder 2, worin der Fluidströmungsweg schraubenförmig ausgebildet ist.
  4. System nach einem der vorangehenden Ansprüche, worin die Transporteinrichtungen (60) den zweiten Probenanteil aus einem Probenbehälter unter Verwendung derselben Ansaugeinrichtungen, welche den ersten Probenanteil ansaugen, erhalten.
  5. Verfahren für ein selektives Erzeugen eines Blutabstrichs, in welchem eine Blutprobe aus einem Behälter angesaugt und analysiert wird, um bestimmte Blutmerkmale zu detektieren, und ein Blutabstrich der Probe auf einem Mikroskop-Objektträger erzeugt wird, wobei das Verfahren die Schritte umfaßt:
    a) sequentielles Ansaugen von ersten und zweiten Blutproben aus demselben Abschnitt bzw. Bereich des Behälters;
    b) Analysieren der ersten Blutprobe zur Detektion dieser Merkmale; und
    c) selektives Erzeugen eines Blutabstrichs der zweiten Blutprobe als Antwort auf die Resultate der Analyse der ersten Blutprobe, worin die zweite Blutprobe gespeichert wird und die Homogenität und die Zellmerkmale der zweiten Blutprobe erhalten werden, während die erste Blutprobe analysiert wird.
  6. Verfahren nach Anspruch 5, worin die zweite Blutprobe in Bewegung gehalten wird, während die erste Blutprobe analysiert wird.
  7. Verfahren nach Anspruch 6, worin während des Speicherschritts die zweite Blutprobe sequentiell in einer Leitung in entgegengesetzten Richtungen bewegt wird.
  8. Verfahren nach Anspruch 7, in welchem die Leitung eine schraubenförmige Form aufweist, um zu bewirken, daß Blutzellen in der zweiten Blutprobe kontinuierlich taumeln bzw. sich bewegen.
EP96937023A 1995-11-14 1996-10-25 System zur blutanalyse, mit mitteln zur aufbewahrung und zum transport des blutes, sowie zur selbsttätigen herstellung von blutauftragsproben Expired - Lifetime EP0861431B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/557,229 US5665312A (en) 1995-11-14 1995-11-14 Blood analysis system having blood storage, transport and automatic slide-making capabilities
US557229 1995-11-14
PCT/US1996/017225 WO1997018457A1 (en) 1995-11-14 1996-10-25 Blood analysis system having blood storage, transport and automatic slide-making capabilities

Publications (2)

Publication Number Publication Date
EP0861431A1 EP0861431A1 (de) 1998-09-02
EP0861431B1 true EP0861431B1 (de) 2000-03-29

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US (1) US5665312A (de)
EP (1) EP0861431B1 (de)
JP (1) JP3650126B2 (de)
AU (1) AU7479196A (de)
DE (1) DE69607476T2 (de)
WO (1) WO1997018457A1 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8815537B2 (en) 2008-04-25 2014-08-26 Roche Diagnostics Hematology, Inc. Method for determining a complete blood count on a white blood cell differential count
US9083857B2 (en) 2008-04-25 2015-07-14 Roche Diagnostics Hematology, Inc. Systems and methods for analyzing body fluids

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6083762A (en) * 1996-05-31 2000-07-04 Packard Instruments Company Microvolume liquid handling system
US5930791A (en) * 1996-12-09 1999-07-27 Leu; Sean Computerized blood analyzer system for storing and retrieving blood sample test results from symmetrical type databases
US5947911A (en) * 1997-01-09 1999-09-07 Via Medical Corporation Method and apparatus for reducing purge volume in a blood chemistry monitoring system
DE69819996T2 (de) * 1997-09-27 2004-09-02 Horiba Ltd. Gerät für die Zählung von Blutzellen und zur immunologischen Bestimmung unter Verwendung von Vollblut
US6407858B1 (en) 1998-05-14 2002-06-18 Genetic Microsystems, Inc Focusing of microscopes and reading of microarrays
US6428752B1 (en) 1998-05-14 2002-08-06 Affymetrix, Inc. Cleaning deposit devices that form microarrays and the like
US6722395B2 (en) 1998-01-13 2004-04-20 James W. Overbeck Depositing fluid specimens on substrates, resulting ordered arrays, techniques for analysis of deposited arrays
US6269846B1 (en) 1998-01-13 2001-08-07 Genetic Microsystems, Inc. Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays
US7095032B2 (en) * 1998-03-20 2006-08-22 Montagu Jean I Focusing of microscopes and reading of microarrays
US6830729B1 (en) 1998-05-18 2004-12-14 University Of Washington Sample analysis instrument
AU3771599A (en) 1998-05-18 1999-12-06 University Of Washington Liquid analysis cartridge
US6572824B1 (en) * 1998-09-18 2003-06-03 Cytyc Corporation Method and apparatus for preparing cytological specimens
US6562299B1 (en) * 1998-09-18 2003-05-13 Cytyc Corporation Method and apparatus for preparing cytological specimens
AU4350501A (en) † 2000-03-08 2001-09-17 Cytyc Corp Method and apparatus for preparing cytological specimens
US6548303B2 (en) * 2000-06-23 2003-04-15 Cem Corporation Method and apparatus for rapid fat content determination
US7220591B2 (en) 2001-12-04 2007-05-22 Cem Corporation Method and apparatus for rapid fat content determination
US7368080B2 (en) * 2002-01-18 2008-05-06 Sysmex Corporation Smear preparing apparatus
US8449822B2 (en) * 2002-01-18 2013-05-28 Sysmex Corporation Smear preparing apparatuses and methods of preparing sample smears
EP1503202B1 (de) * 2003-07-31 2018-07-25 Sysmex Corporation Vorrichtung zur Vorbereitung von Abstrichen
DE10342304B4 (de) * 2003-09-12 2006-02-02 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto Rüssel zur Entnahme und/oder Zufuhr einer Flüssigkeit
EP1612537B1 (de) * 2004-06-30 2012-12-19 Sysmex Corporation Vorrichtung zur Vorbereitung von Proben, System zur Analyse von Proben und Probenplatte
US7300804B2 (en) * 2004-11-15 2007-11-27 Beckman Coulter, Inc. Method and apparatus for controlling the uniform smearing of a biological liquid over a substrate
JP5211426B2 (ja) * 2006-01-23 2013-06-12 株式会社日立プラントテクノロジー マイクロリアクタシステム
JP4975599B2 (ja) * 2007-12-17 2012-07-11 シスメックス株式会社 標本作製装置および標本作製方法
CN101487845B (zh) * 2009-02-19 2013-01-02 苏州海路生物技术有限公司 大便标本自动检测仪
US8236256B2 (en) * 2010-04-27 2012-08-07 Thomas Friedlander Apparatus and method for efficient and precise transfer of liquids
US9111343B2 (en) 2011-01-18 2015-08-18 Roche Diagnostics Hematology, Inc. Microscope slide coordinate system registration

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1454510A (en) * 1973-05-22 1976-11-03 Tetronics Research Dev Co Ltd Laying blood traces
CA1057592A (en) * 1975-12-19 1979-07-03 Technicon Instruments Corporation Transfer process and apparatus therefor
US4016828A (en) * 1976-03-22 1977-04-12 The Perkin-Elmer Corporation Apparatus for blood film preparation
US4096824A (en) * 1976-07-09 1978-06-27 Smithkline Corporation Slide smearing device
US4303611A (en) * 1980-08-11 1981-12-01 Eastman Kodak Company Analyzer apparatus featuring a simplified incubator
US4378333A (en) * 1980-12-11 1983-03-29 Laipply Thomas C Device for preparing blood smears on glass slides and method therefor
US4494479A (en) * 1982-10-14 1985-01-22 Coulter Electronics, Inc. Slide preparation apparatus
US4902476A (en) * 1983-01-14 1990-02-20 Baxter International Inc. Heat exchanger and blood oxygenator apparatus
JPS60162955A (ja) * 1984-02-03 1985-08-24 Hitachi Ltd 血球自動分析装置
US4997627A (en) * 1987-07-17 1991-03-05 Fisher Scientific Company Sample analysis
US5073500A (en) * 1988-01-08 1991-12-17 Inax Corporation Method and apparatus for detecting urinary constituents
US5209903A (en) * 1989-09-06 1993-05-11 Toa Medical Electronics, Co., Ltd. Synthetic apparatus for inspection of blood
AU636384B2 (en) * 1989-09-06 1993-04-29 Toa Medical Electronics Co., Ltd. Synthetic apparatus for inspection of blood
JP3077772B2 (ja) * 1991-06-05 2000-08-14 シスメックス株式会社 複数の分析モジュールを用いる粒子自動分析方法及び装置
JP2812625B2 (ja) * 1992-10-19 1998-10-22 株式会社日立製作所 液体試料自動分析装置
JP2842758B2 (ja) * 1993-05-10 1999-01-06 株式会社日立製作所 自動分析装置
JP2948069B2 (ja) * 1993-09-20 1999-09-13 株式会社日立製作所 化学分析装置
US5494828A (en) * 1994-07-13 1996-02-27 Leopando; Mark E. Slide dispensing device and method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8815537B2 (en) 2008-04-25 2014-08-26 Roche Diagnostics Hematology, Inc. Method for determining a complete blood count on a white blood cell differential count
US9017610B2 (en) 2008-04-25 2015-04-28 Roche Diagnostics Hematology, Inc. Method of determining a complete blood count and a white blood cell differential count
US9083857B2 (en) 2008-04-25 2015-07-14 Roche Diagnostics Hematology, Inc. Systems and methods for analyzing body fluids
US9602777B2 (en) 2008-04-25 2017-03-21 Roche Diagnostics Hematology, Inc. Systems and methods for analyzing body fluids
US10094764B2 (en) 2008-04-25 2018-10-09 Roche Diagnostics Hematology, Inc. Systems and methods for determining a complete blood count and a white blood cell differential count

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DE69607476T2 (de) 2000-12-21
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US5665312A (en) 1997-09-09
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AU7479196A (en) 1997-06-05
WO1997018457A1 (en) 1997-05-22
DE69607476D1 (de) 2000-05-04

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