EA202092684A1 - Применение рамановской спектроскопии для последующей очистки - Google Patents

Применение рамановской спектроскопии для последующей очистки

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Publication number
EA202092684A1
EA202092684A1 EA202092684A EA202092684A EA202092684A1 EA 202092684 A1 EA202092684 A1 EA 202092684A1 EA 202092684 A EA202092684 A EA 202092684A EA 202092684 A EA202092684 A EA 202092684A EA 202092684 A1 EA202092684 A1 EA 202092684A1
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EA
Eurasian Patent Office
Prior art keywords
raman spectroscopy
protein purification
application
situ raman
intermediates
Prior art date
Application number
EA202092684A
Other languages
English (en)
Inventor
Кристина Пассно
Кристофер Коуэн
Эндрю Тастиан
Original Assignee
Ридженерон Фармасьютикалз, Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ридженерон Фармасьютикалз, Инк. filed Critical Ридженерон Фармасьютикалз, Инк.
Publication of EA202092684A1 publication Critical patent/EA202092684A1/ru

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4044—Concentrating samples by chemical techniques; Digestion; Chemical decomposition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/65—Raman scattering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration

Abstract

Предлагаются способы и системы рамановской спектроскопии in situ для характеризации или количественной оценки промежуточного продукта очистки белка и/или конечного концентрированного пула в процессе производства или изготовления. В одном варианте осуществления данного изобретения рамановскую спектроскопию in situ применяют для характеризации или количественной оценки критически важных показателей качества промежуточных продуктов очистки белка во время последующей обработки (т.е. после сбора промежуточного продукта очистки белка). Например, описанные способы и системы рамановской спектроскопии in situ могут применяться для характеризации и количественной оценки промежуточных продуктов очистки белка, при этом промежуточные продукты очистки белка являются очищенными, конденсированными или иным образом включены в состав готового лекарственного препарата для продажи или введения.
EA202092684A 2018-08-27 2019-08-26 Применение рамановской спектроскопии для последующей очистки EA202092684A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862723188P 2018-08-27 2018-08-27
PCT/US2019/048100 WO2020046793A1 (en) 2018-08-27 2019-08-26 Use of raman spectroscopy in downstream purification

Publications (1)

Publication Number Publication Date
EA202092684A1 true EA202092684A1 (ru) 2021-03-11

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA202092684A EA202092684A1 (ru) 2018-08-27 2019-08-26 Применение рамановской спектроскопии для последующей очистки

Country Status (13)

Country Link
US (2) US11358984B2 (ru)
EP (1) EP3774841A1 (ru)
JP (1) JP2021535739A (ru)
KR (1) KR20210052389A (ru)
CN (1) CN112218877A (ru)
AU (1) AU2019329686A1 (ru)
BR (1) BR112020024296A2 (ru)
CA (1) CA3100035A1 (ru)
EA (1) EA202092684A1 (ru)
IL (1) IL280880A (ru)
MX (1) MX2021002279A (ru)
SG (1) SG11202011970TA (ru)
WO (1) WO2020046793A1 (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4317170A1 (en) 2021-03-30 2024-02-07 FUJIFILM Corporation Method for estimating purified state
TW202326113A (zh) * 2021-10-27 2023-07-01 美商安進公司 使用光譜學進行的基於深度學習的預測
WO2024070543A1 (ja) * 2022-09-27 2024-04-04 富士フイルム株式会社 情報処理装置、情報処理装置の作動方法、情報処理装置の作動プログラム、並びに状態予測モデル

Family Cites Families (155)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5675931A (en) 1992-10-02 1997-10-14 Wasserman; Kurt J. Plant tender
WO1997036540A1 (en) 1993-11-30 1997-10-09 Florida Institute Of Technology Determination of concentrations of biological substances using raman spectroscopy and artificial neural network discriminator
WO1998041611A1 (en) 1997-03-20 1998-09-24 Regents Of The University Of Minnesota Process for the continuous culture of cells
US7057732B2 (en) 1999-01-25 2006-06-06 Amnis Corporation Imaging platform for nanoparticle detection applied to SPR biomolecular interaction analysis
US20010044129A1 (en) 2000-03-14 2001-11-22 Jian Ling Methodology of using raman imaging microscopy for evaluating drug action within living cells
DE10026195A1 (de) 2000-05-26 2001-11-29 Merck Patent Gmbh Verfahren und Vorrichtung zum Identifizieren chemischer Substanzen
DE10217948B4 (de) 2002-04-22 2007-04-05 Kiefer, Wolfgang, Prof. Dr. Verfahren und Vorrichtung zur Erstellung Raman- und SER-spektroskopischer Messungen biologischer und chemischer Proben
DK1623019T4 (en) 2003-05-15 2017-03-27 Wyeth Llc LIMITED GLUCOSE SUPPLY TO ANIMAL CELL CULTURE
US7271896B2 (en) 2003-12-29 2007-09-18 Intel Corporation Detection of biomolecules using porous biosensors and raman spectroscopy
US20060281068A1 (en) 2005-06-09 2006-12-14 Chemimage Corp. Cytological methods for detecting a disease condition such as malignancy by Raman spectroscopic imaging
US7586601B2 (en) 2005-06-14 2009-09-08 Ebstein Steven M Applications of laser-processed substrate for molecular diagnostics
DK1946083T3 (da) 2005-10-17 2011-09-19 Sword Diagnostics Inc Fremgangsmåde til detektion af biologiske organismer under anvendelse af ramanspredning
CA2652976C (en) 2006-06-02 2015-08-11 Regeneron Pharmaceuticals, Inc. High affinity antibodies to human il-6 receptor
WO2008036600A2 (en) 2006-09-18 2008-03-27 Genentech, Inc. Methods of protein production
US7608693B2 (en) 2006-10-02 2009-10-27 Regeneron Pharmaceuticals, Inc. High affinity human antibodies to human IL-4 receptor
US7879984B2 (en) 2007-07-31 2011-02-01 Regeneron Pharmaceuticals, Inc. Human antibodies to human CD20 and method of using thereof
US8309088B2 (en) 2007-08-10 2012-11-13 Regeneron Pharmaceuticals, Inc. Method of treating osteoarthritis with an antibody to NGF
EP2285950A1 (en) 2008-06-13 2011-02-23 FOSS Analytical A/S Process control of biotechnological processes
EP2331700A2 (en) 2008-08-08 2011-06-15 Biogen Idec MA Inc. Nutrient monitoring and feedback control for increased bioproduct production
US20100136609A1 (en) 2008-10-31 2010-06-03 Biomerieux, Inc. Method for separation, characterization and/or identification of microorganisms using raman spectroscopy
JO3672B1 (ar) 2008-12-15 2020-08-27 Regeneron Pharma أجسام مضادة بشرية عالية التفاعل الكيماوي بالنسبة لإنزيم سبتيليسين كنفرتيز بروبروتين / كيكسين نوع 9 (pcsk9).
CN101482554B (zh) 2009-01-22 2012-10-24 东北师范大学 用于生物分离和检测的磁性拉曼纳米复合材料及其制备方法
CN101482509A (zh) 2009-03-03 2009-07-15 福建师范大学 表面增强拉曼光谱检测动物活性单细胞样品处理方法
EP2414536B1 (en) 2009-04-03 2014-06-18 Battelle Memorial Institute Fluorescence cued raman identification of viable organisms
US20100291599A1 (en) 2009-05-18 2010-11-18 Bruker Optics, Inc. Large area scanning apparatus for analyte quantification by surface enhanced raman spectroscopy and method of use
MY164121A (en) 2009-06-26 2017-11-30 Regeneron Pharma Readily isolated bispecific antibodies with native immunoglobulin format
WO2011055791A1 (ja) 2009-11-05 2011-05-12 株式会社日立ハイテクノロジーズ 細菌コロニー釣菌装置及びその方法
US20130177972A1 (en) 2009-11-17 2013-07-11 David Green Bioreactors, systems, and methods for producing and/or analyzing organs
JO3417B1 (ar) 2010-01-08 2019-10-20 Regeneron Pharma الصيغ المستقرة التي تحتوي على الأجسام المضادة لمضاد مستقبل( interleukin-6 (il-6r
JO3340B1 (ar) 2010-05-26 2019-03-13 Regeneron Pharma مضادات حيوية لـعامل تمايز النمو 8 البشري
DE102010023099B3 (de) 2010-06-09 2011-11-17 Celltool Gmbh Verfahren und Vorrichtung zum Charakterisieren von biologischen Objekten
US9025850B2 (en) 2010-06-25 2015-05-05 Cireca Theranostics, Llc Method for analyzing biological specimens by spectral imaging
JOP20190250A1 (ar) 2010-07-14 2017-06-16 Regeneron Pharma صيغ مستقرة تحتوي على الأجسام المضادة لمضاد عامل نمو الأعصاب
WO2012037430A1 (en) 2010-09-17 2012-03-22 Abbott Laboratories Raman spectroscopy for bioprocess operations
AU2011305754B2 (en) 2010-09-20 2015-11-05 Abbvie Inc. Purification of antibodies using simulated moving bed chromatography
AR083044A1 (es) 2010-09-27 2013-01-30 Regeneron Pharma Anticuerpos anti-cd48 y usos de los mismos
CA2813587C (en) 2010-10-06 2019-01-15 Regeneron Pharmaceuticals, Inc. Stabilized formulations containing anti-interleukin-4 receptor (il-4r) antibodies
JO3756B1 (ar) 2010-11-23 2021-01-31 Regeneron Pharma اجسام مضادة بشرية لمستقبلات الجلوكاجون
WO2012122413A1 (en) 2011-03-08 2012-09-13 University Of Maryland Baltimore County Microscale bioprocessing system and method for protein manufacturing
US10774304B2 (en) 2011-03-08 2020-09-15 University Of Maryland, Baltimore County System and method for production of on-demand proteins in a portable unit for point of care delivery
JO3412B1 (ar) 2011-06-17 2019-10-20 Regeneron Pharma أجسام مضادة ل angptl3 واستخداماتها
JP6236005B2 (ja) 2011-08-30 2017-11-22 バテル メモリアル インスティチュート バイオテクノロジー生産におけるラマン分光法を用いたマイコプラズマ汚染の識別
US10421939B2 (en) 2011-10-24 2019-09-24 Bend Research, Inc. Systems and methods for producing bioproducts
DK2780368T3 (en) 2011-11-14 2018-02-05 Regeneron Pharma COMPOSITIONS AND PROCEDURES FOR INCREASING MUSCLE MASS AND MUSCLE STRENGTH BY SPECIFIC ANTAGONIZATION OF GDF8 AND / OR ACTIVIN A
EA033790B1 (ru) 2011-12-19 2019-11-26 Opticul Diagnostics Ltd Способ спектрального обнаружения и идентификации микроорганизмов в культуре
NZ627859A (en) 2012-01-23 2015-09-25 Regeneron Pharma Stabilized formulations containing anti-ang2 antibodies
US8477307B1 (en) 2012-01-26 2013-07-02 Ann Rachel Yufa Methods and apparatus for biomedical agent multi-dimension measuring and analysis
US10101209B2 (en) 2012-04-30 2018-10-16 Finesse Solutions, Inc. Method and apparatus for quantifying solutions comprised of multiple analytes
JO3820B1 (ar) 2012-05-03 2021-01-31 Regeneron Pharma أجسام مضادة بشرية لـ fel d1وطرق لاستخدامها
TW201843172A (zh) 2012-06-25 2018-12-16 美商再生元醫藥公司 抗－ｅｇｆｒ抗體及其用途
EA028244B1 (ru) 2012-08-13 2017-10-31 Ридженерон Фармасьютикалз, Инк. АНТИТЕЛА К PCSK9 C pH-ЗАВИСИМЫМИ ХАРАКТЕРИСТИКАМИ СВЯЗЫВАНИЯ
WO2014027652A1 (ja) 2012-08-17 2014-02-20 独立行政法人科学技術振興機構 ラマン分光法を用いた生体分子の解析方法及び装置
US9512214B2 (en) 2012-09-02 2016-12-06 Abbvie, Inc. Methods to control protein heterogeneity
JOP20200236A1 (ar) 2012-09-21 2017-06-16 Regeneron Pharma الأجسام المضادة لمضاد cd3 وجزيئات ربط الأنتيجين ثنائية التحديد التي تربط cd3 وcd20 واستخداماتها
US9506867B2 (en) 2012-12-11 2016-11-29 Biogen Ma Inc. Spectroscopic analysis of nutrient materials for use in a cell culture process
JO3405B1 (ar) 2013-01-09 2019-10-20 Regeneron Pharma الأجسام المضادة لمضاد مستقبل عامل النمو المشتق من الصفائح الدموية - بيتا واستخداماتها
US20140212867A1 (en) 2013-01-30 2014-07-31 Hewlett-Packard Development Company, L.P. Device for monitoring and controlling cellular growth
WO2014137291A1 (en) 2013-03-05 2014-09-12 Agency For Science, Technology And Research Method for detecting analyte using surface enhanced raman spectroscopy, biosensor, and method of manufacturing thereof
JO3532B1 (ar) 2013-03-13 2020-07-05 Regeneron Pharma الأجسام المضادة لمضاد انترلوكين-33 واستعمالاتها
TWI659968B (zh) 2013-03-14 2019-05-21 再生元醫藥公司 針對呼吸道融合病毒ｆ蛋白質的人類抗體及其使用方法
DK2972238T3 (da) 2013-03-15 2023-06-19 Biogen Ma Inc Anvendelse af raman-spektroskopi til monitorering af dyrkningsmedium
CN105007929B (zh) 2013-03-15 2019-05-10 瑞泽恩制药公司 Il-33拮抗剂和其用途
SG11201509158SA (en) 2013-04-05 2015-12-30 Nikon Corp Cell observation method, cell observation apparatus, cell observation program, cell sheet manufacturing method, and cell sheet manufacturing apparatus
GB2514541B (en) 2013-04-10 2016-09-07 Dublin Inst Of Tech Cervical sample preparation for reduced variability in Raman spectroscopy
GB201307013D0 (en) 2013-04-18 2013-05-29 Renishaw Diagnostics Ltd Seers based assays for oligonucleotides
US11408892B2 (en) 2013-06-18 2022-08-09 The Trustees Of Columbia University In The City Of New York Devices, compositions and methods for imaging with raman scattering
TWI641620B (zh) 2013-08-21 2018-11-21 再生元醫藥公司 抗－ｐｒｌｒ抗體及其用途
US9085618B2 (en) * 2013-10-18 2015-07-21 Abbvie, Inc. Low acidic species compositions and methods for producing and using the same
WO2015095255A1 (en) 2013-12-20 2015-06-25 Bp Corporation North America Inc. Method of monitoring and controlling a bioprocess using near- and mid-infrared spectroscopy
TWI680138B (zh) 2014-01-23 2019-12-21 美商再生元醫藥公司 抗ｐｄ-ｌ１之人類抗體
TWI681969B (zh) 2014-01-23 2020-01-11 美商再生元醫藥公司 針對ｐｄ-１的人類抗體
US9540701B2 (en) 2014-02-28 2017-01-10 Asl Analytical, Inc. Apparatus and method for automated process monitoring and control with near infrared spectroscopy
HUE045307T2 (hu) 2014-03-11 2019-12-30 Regeneron Pharma EGFRVIII-ellenes antitestek és alkalmazásaik
US10544395B2 (en) 2014-03-19 2020-01-28 Pfizer Inc. Method of cell culture
TWI701042B (zh) 2014-03-19 2020-08-11 美商再生元醫藥公司 用於腫瘤治療之方法及抗體組成物
CN106030278A (zh) 2014-03-25 2016-10-12 马尔文仪器有限公司 分散在液相中的蛋白质的拉曼光谱结构调查研究
KR102461511B1 (ko) 2014-05-05 2022-11-01 리제너론 파마슈티칼스 인코포레이티드 인간화된 c5 및 c3 동물
JO3701B1 (ar) 2014-05-23 2021-01-31 Regeneron Pharma مضادات حيوية بشرية لمتلازمة الشرق الأوسط التنفسية - بروتين كورونا فيروس الشوكي
US10563163B2 (en) 2014-07-02 2020-02-18 Biogen Ma Inc. Cross-scale modeling of bioreactor cultures using Raman spectroscopy
KR20170062466A (ko) 2014-09-16 2017-06-07 리제너론 파마슈티칼스 인코포레이티드 항-글루카곤 항체 및 그것의 사용
CN104297225B (zh) 2014-09-29 2018-02-16 无限极（中国）有限公司 一种细胞内黑色素的快速检测与成像的方法
KR102395773B1 (ko) 2014-11-07 2022-05-09 삼성전자주식회사 생체 시료의 분석 시스템 및 이를 이용한 분석 방법
TWI506265B (zh) 2014-11-19 2015-11-01 Nat Univ Chung Cheng 微流式生物感測系統
TWI710573B (zh) 2015-01-26 2020-11-21 美商再生元醫藥公司 抗伊波拉病毒醣蛋白之人類抗體
JP2016156807A (ja) * 2015-02-23 2016-09-01 株式会社堀場製作所 分析方法及び分析装置
CN104774756A (zh) 2015-04-23 2015-07-15 东南大学 一种基于sers检测技术的微流控药物筛选芯片及方法
CN106295251A (zh) 2015-05-25 2017-01-04 中国科学院青岛生物能源与过程研究所 基于单细胞表现型数据库的表型数据分析处理方法
US20180291329A1 (en) * 2015-05-29 2018-10-11 Biogen Ma Inc. Cell culture methods and systems
JPWO2016208356A1 (ja) 2015-06-26 2018-05-24 株式会社ニコン 判定装置、判定プログラム、判定方法、細胞シート製造装置、および細胞シート製造方法
JP7001585B2 (ja) 2015-09-23 2022-02-04 ファイザー・インク 細胞および細胞培養の方法
EP3380825B1 (de) 2015-11-23 2020-09-23 CellTool GmbH Vorrichtung und verfahren zum analysieren biologischer objekte mit raman spektroskopie
CN105738343A (zh) 2016-03-03 2016-07-06 福建师范大学 采用表面增强显微拉曼光谱检测咽拭标本生化成分的方法
SG11201807124PA (en) 2016-03-23 2018-09-27 Agency Science Tech & Res Surface enhanced raman spectroscopy (sers) microfluidics biosensor for detecting single and/or multiple analytes
KR102610590B1 (ko) 2016-07-25 2023-12-07 삼성전자주식회사 생체 내 물질 추정 장치 및 방법, 단위 스펙트럼 획득 장치 및 웨어러블 기기
WO2018031954A1 (en) 2016-08-12 2018-02-15 Biogen Ma Inc. Identifying components of dry powder mixtures using raman spectroscopy
CN106645079B (zh) 2016-09-30 2019-07-23 福建师范大学 一种基于红细胞光镊拉曼光谱技术的人体血型鉴别方法
CN106769693A (zh) 2016-11-14 2017-05-31 中国科学院重庆绿色智能技术研究院 一种基于拉曼光谱的循环肿瘤细胞自动检测系统
GB201621912D0 (en) 2016-12-21 2017-02-01 Dublin Inst Of Tech And Trinity College Dublin A method of using raman spectroscopy for identification of low grade cervical cytology cases likely to progress to high grade / cancer
US10261020B2 (en) 2017-01-04 2019-04-16 Kaiser Optical Systems Inc. Cost-effective Raman probe assembly for single-use bioreactor vessels
CN112014372A (zh) 2017-02-20 2020-12-01 中国科学院青岛生物能源与过程研究所 一种快速定性/定量评价不同抗菌药物作用效果的方法
US20180261329A1 (en) 2017-02-21 2018-09-13 Segterra, Inc. Personalized Health-Information Based on Genetic Data
JPWO2018159833A1 (ja) 2017-03-02 2020-01-16 株式会社ニコン 細胞の判別方法、がんの検査方法、計測装置、がんの検査装置および検査プログラム
US11105747B2 (en) 2017-03-20 2021-08-31 Spectral Platforms, Inc. Spectroscopic methods to detect and characterize microorganisms
CN107132208B (zh) 2017-04-10 2018-11-30 苏州贝康医疗器械有限公司 一种基于拉曼光谱测量的细胞培养液质量检测方法
DE102017207262B4 (de) 2017-04-28 2019-12-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren und Vorrichtung zur Bereitstellung einer eine gewünschte Zielprotein-Expression aufweisenden Zelllinie
US11598712B2 (en) 2017-05-02 2023-03-07 Thinkcyte, Inc. System and method for cell evaluation, and cell evaluation program
EP3679121A1 (en) 2017-09-07 2020-07-15 Corning Incorporated Optical cell culture monitoring and analyte measuring system
CN107741417B (zh) 2017-09-28 2019-11-08 上海氘峰医疗器械有限公司 一种原位快速检测细胞生物过程的方法
CN111344558A (zh) 2017-10-06 2020-06-26 龙沙有限公司 使用拉曼光谱法自动控制细胞培养
EP3698125A1 (en) 2017-10-16 2020-08-26 Regeneron Pharmaceuticals, Inc. In situ raman spectroscopy systems and methods for controlling process variables in cell cultures
AR113449A1 (es) 2017-10-16 2020-05-06 Regeneron Pharma Sistemas y métodos de espectroscopía de raman in situ para controlar variables de procesos en cultivos celulares
SG11202002339XA (en) 2017-10-16 2020-04-29 Regeneron Pharma Perfusion bioreactor and related methods of use
KR20190054746A (ko) 2017-11-14 2019-05-22 중앙대학교 산학협력단 줄기 세포 분석 방법
EP3714035A4 (en) 2017-11-22 2021-08-18 Culture Biosciences, Inc. FERMENTATION AUTOMATION WORK CELL
CN108037107A (zh) 2017-12-04 2018-05-15 中国科学院长春光学精密机械与物理研究所 一种同步检测装置
EP3709005A1 (en) 2017-12-13 2020-09-16 HORIBA, Ltd. Distinguishing method, learning method, distinguishing device and computer program
CN108169203B (zh) 2017-12-14 2020-09-08 济南大学 一种检测hOGG1活性的生物传感器及其应用
EP3744825A4 (en) 2018-01-24 2021-12-08 Yokogawa Electric Corporation CULTURE ADDITIVE DELIVERY APPARATUS, CULTURE CONTAINER, CULTURE SYSTEM AND CELL PRODUCTION METHOD
CN108267437B (zh) 2018-02-09 2020-06-23 中国科学院城市环境研究所 一种基于单细胞拉曼光谱和15n2稳定同位素标记的固氮菌检测方法
EP3752593B1 (en) 2018-02-12 2021-12-01 Corning Incorporated Remote monitoring system for cell culture
EP3910320A1 (en) 2018-03-29 2021-11-17 The Automation Partnership (Cambridge) Ltd. Computer-implemented method and system for spectroscopic analysis of biological material
DE102018108325B4 (de) 2018-04-09 2020-07-09 Schott Ag Sensoraufnahme für einen Bioreaktor sowie Bioreaktor mit Sensoraufnahme und Verfahren zur Vermehrung oder Kultivierung biologischen Materials
CN112020508A (zh) 2018-05-04 2020-12-01 法国多肽实验室 具有再循环回路的自动合成反应器系统
KR20180059739A (ko) 2018-05-28 2018-06-05 울산과학기술원 라만분광기반 바이오센서의 제조방법
CN110687089B (zh) 2018-07-06 2021-12-10 中国科学院青岛生物能源与过程研究所 基于单细胞检测的高通量并行拉曼光谱仪
US11959836B2 (en) 2018-08-15 2024-04-16 Georgia Tech Research Corporation Analysis system and methods of use thereof
CN109266717B (zh) 2018-09-27 2022-02-22 上海镭立激光科技有限公司 一种通过单细胞分析检测细菌耐药性的方法和装置
AU2019365102A1 (en) 2018-10-23 2021-04-29 Amgen Inc. Automatic calibration and automatic maintenance of Raman spectroscopic models for real-time predictions
WO2020087040A1 (en) 2018-10-26 2020-04-30 Pataigin, Llc Method for analyzing samples
EP3859008B1 (en) 2018-11-02 2022-11-16 PHC Holdings Corporation Method for estimating cell count, and device for estimating cell count
CN109342393A (zh) 2018-11-15 2019-02-15 中牧实业股份有限公司 一种利用拉曼光谱检测细胞培养基中葡萄糖含量的方法
CN111220590A (zh) 2018-11-23 2020-06-02 上海氘峰医疗器械有限公司 病原微生物药敏的快速检测仪器及检测方法
CN111220589A (zh) 2018-11-23 2020-06-02 上海氘峰医疗器械有限公司 病原微生物的快速鉴定仪器及鉴定方法
CN209460143U (zh) 2018-11-23 2019-10-01 上海氘峰医疗器械有限公司 病原微生物的快速鉴定仪器
CN113728110A (zh) 2018-12-21 2021-11-30 克维拉公司 用于微集落生长和微生物细胞表征的系统和方法
CN109342403A (zh) 2018-12-21 2019-02-15 福建师范大学 基于表面增强拉曼光谱检测细胞脱颗粒的方法
JP2022049724A (ja) 2019-02-08 2022-03-30 国立大学法人 筑波大学 細胞種の推定方法、細胞種の推定装置、細胞の製造方法、細胞の製造装置、観察方法、観察装置、学習済モデルの製造方法、および学習済モデルの製造装置
KR102291105B1 (ko) 2019-03-04 2021-08-23 주식회사 엑소퍼트 엑소좀에 의한 인공지능 기반의 액체생검을 이용한 암 진단 정보 제공 방법 및 시스템
WO2020186026A1 (en) 2019-03-12 2020-09-17 The Trustees Of Columbia University In The City Of New York Stimulated raman coupled fluorescence spectroscopy and microscopy system and method
CN110208238A (zh) 2019-03-27 2019-09-06 天津理工大学 一种基于svm模型结合图像对肺癌组织的精确定位方法
US20220187209A1 (en) 2019-05-07 2022-06-16 President And Fellows Of Harvard College In situ measurement of absolute concentrations by normalized raman imaging
CN114245869A (zh) 2019-05-23 2022-03-25 百时美施贵宝公司 监测细胞培养基的方法
CN113924355B (zh) 2019-05-28 2024-04-02 上海药明生物技术有限公司 用于监测和自动控制灌流细胞培养的拉曼光谱集成灌流细胞培养系统
WO2020247952A2 (en) 2019-06-07 2020-12-10 The Regents Of The University Of California Systems comprising substrates and methods of using the same for detection of pancreatic cancers
GB201909082D0 (en) 2019-06-25 2019-08-07 Ge Healthcare Bio Sciences Ab Methods for control of a bioprocess
GB201909274D0 (en) 2019-06-27 2019-08-14 Ge Healthcare Bio Sciences Ab Method in bioprocess purification system
JP7257277B2 (ja) 2019-07-12 2023-04-13 株式会社日立製作所 細胞培養モニタリング装置及び細胞培養システム
MX2022001519A (es) 2019-08-05 2022-04-06 Seer Inc Sistemas y metodos para preparacion de muestras, generacion de datos y analisis de proteina corona.
WO2021030672A1 (en) 2019-08-14 2021-02-18 The University Of Massachusetts Cell culture methods
US20210062133A1 (en) 2019-08-28 2021-03-04 Nirrin Technologies, Inc. Device and bioreactor monitoring system and method
CN110501319A (zh) 2019-08-29 2019-11-26 中国科学院长春应用化学研究所 多通路结构光照明的拉曼超分辨显微成像方法
CN110441287B (zh) 2019-09-11 2022-02-08 重庆工商大学 一种原位定量检测细菌信号分子的增强拉曼光谱方法
CN110646401B (zh) 2019-10-12 2022-04-12 福建师范大学 基于羟基磷灰石纳米粒子吸附蛋白的sers检测方法
CN111289489B (zh) 2020-03-05 2023-06-02 长春长光辰英生物科学仪器有限公司 一种基于拉曼光谱的微生物单细胞生长检测方法
CN111912826B (zh) 2020-06-22 2024-03-01 上海氘峰医疗科技有限公司 一种在细胞水平上进行抗肿瘤药物药效评估的方法

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