EA201890121A1 - Способ пролиферации естественных клеток-киллеров и композиции для пролиферации естественных клеток-киллеров - Google Patents

Способ пролиферации естественных клеток-киллеров и композиции для пролиферации естественных клеток-киллеров

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Publication number
EA201890121A1
EA201890121A1 EA201890121A EA201890121A EA201890121A1 EA 201890121 A1 EA201890121 A1 EA 201890121A1 EA 201890121 A EA201890121 A EA 201890121A EA 201890121 A EA201890121 A EA 201890121A EA 201890121 A1 EA201890121 A1 EA 201890121A1
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Eurasian Patent Office
Prior art keywords
chillers
proliferation
natural cell
compositions
interleukin
Prior art date
Application number
EA201890121A
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English (en)
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EA038848B1 (ru
Inventor
Ён Сок Бэк
Ми Кён Чой
Ю Ра Кан
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Ча Биотек Ко., Лтд.
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Application filed by Ча Биотек Ко., Лтд. filed Critical Ча Биотек Ко., Лтд.
Priority claimed from PCT/KR2016/006754 external-priority patent/WO2016209021A1/ko
Publication of EA201890121A1 publication Critical patent/EA201890121A1/ru
Publication of EA038848B1 publication Critical patent/EA038848B1/ru

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2312—Interleukin-12 (IL-12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2318—Interleukin-18 (IL-18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
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  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
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  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к способу пролиферации естественных клеток-киллеров и композиции для пролиферации естественных клеток-киллеров, способных улучшать эффект пролиферации клеток и их противораковое действие, где способ включает в себя стадию культивирования естественных клеток-киллеров в культуральной жидкости, содержащей первый интерлейкин (IL-2), второй интерлейкин (по меньшей мере один интерлейкин выбран из группы, состоящей из IL-12, IL-15 и IL-18) и антитело к NKp46.
EA201890121A 2015-06-24 2016-06-24 Способ пролиферации естественных клеток-киллеров и композиции для пролиферации естественных клеток-киллеров EA038848B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20150089882 2015-06-24
KR1020160078622A KR101909879B1 (ko) 2015-06-24 2016-06-23 자연살해세포의 증식 방법 및 자연살해세포 증식용 조성물
PCT/KR2016/006754 WO2016209021A1 (ko) 2015-06-24 2016-06-24 자연살해세포의 증식 방법 및 자연살해세포 증식용 조성물

Publications (2)

Publication Number Publication Date
EA201890121A1 true EA201890121A1 (ru) 2018-06-29
EA038848B1 EA038848B1 (ru) 2021-10-28

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KR (1) KR101909879B1 (ru)
EA (1) EA038848B1 (ru)

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* Cited by examiner, † Cited by third party
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KR102265437B1 (ko) 2017-11-24 2021-06-15 의료법인 성광의료재단 Nk 배양용 조성물 및 이를 이용하여 nk 세포를 배양하는 방법
KR102014467B1 (ko) * 2017-12-14 2019-08-26 (주)녹십자웰빙 아토피성 피부염, 탈모, 상처 또는 피부 주름의 개선용 화장료 조성물 및 약학 조성물
KR102311057B1 (ko) * 2017-12-14 2021-10-08 (주)녹십자웰빙 아토피성 피부염, 탈모, 상처 또는 피부 주름의 개선용 화장료 조성물 및 약학 조성물
KR102069704B1 (ko) * 2018-05-16 2020-01-23 고려대학교 산학협력단 Hdac 억제제를 이용한 사람 유래 자연살해세포의 확장 배양법
KR102081417B1 (ko) 2018-11-23 2020-02-25 주식회사 이뮤니스바이오 Ｂｔｏ―１의 ｎｋ 세포 증식 용도
JP2022529280A (ja) * 2019-04-17 2022-06-20 チャ バイオテック カンパニー リミテッド 抗癌活性が増大されたナチュラルキラー細胞、及びその免疫治療用途
CN111944754B (zh) * 2020-08-26 2024-04-19 沈阳细胞治疗工程技术研发中心有限公司 一种自然杀伤细胞的培养方法
KR102581230B1 (ko) * 2020-10-16 2023-09-21 의료법인 성광의료재단 항암 관련 유전자 발현이 조절된 자연살해세포 및 이의 용도
CN113832101A (zh) * 2021-09-03 2021-12-24 秦红 自然杀伤细胞体外高效扩增的制备方法
KR20230150113A (ko) * 2022-04-21 2023-10-30 (주) 엘피스셀테라퓨틱스 유사 기억 자연 살해 세포 제조방법 및 이를 이용하여 제조된 유사 기억 자연 살해 세포의 항암 용도
CN115039764B (zh) * 2022-07-14 2024-03-12 北京鼎成肽源生物技术有限公司 一种自然杀伤细胞的冻存方法

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JP2010220479A (ja) * 2007-06-15 2010-10-07 Medeinetto:Kk Ｎｋ細胞の培養方法及びｎｋ細胞の利用
KR101133185B1 (ko) * 2008-07-29 2012-04-06 서울대학교병원 자연살해세포의 증식방법
KR101039843B1 (ko) * 2010-08-30 2011-06-09 주식회사 엔케이바이오 자기활성화 림프구 배양용 배지 조성물 및 이를 이용한 자기활성화 림프구 배양방법
KR101298012B1 (ko) * 2011-02-08 2013-08-26 (주)차바이오앤디오스텍 암세포로의 표적지향을 위한 자연살해 세포를 포함하는 림프구의 제조방법 및 이를 포함하는 약학 조성물
KR101644984B1 (ko) * 2011-12-22 2016-08-12 재단법인 목암생명과학연구소 자연살해세포의 제조방법, 이러한 방법에 의해 제조된 자연살해세포 및 이를 포함하는 종양 및 감염성 질환 치료용 조성물

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KR20170000798A (ko) 2017-01-03
KR101909879B1 (ko) 2018-10-19

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