EA201890121A1 - METHOD OF PROLIFERATION OF NATURAL CELL-CHILLERS AND COMPOSITIONS FOR PROLIFERATION OF NATURAL CELL-CHILLERS - Google Patents

METHOD OF PROLIFERATION OF NATURAL CELL-CHILLERS AND COMPOSITIONS FOR PROLIFERATION OF NATURAL CELL-CHILLERS

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Publication number
EA201890121A1
EA201890121A1 EA201890121A EA201890121A EA201890121A1 EA 201890121 A1 EA201890121 A1 EA 201890121A1 EA 201890121 A EA201890121 A EA 201890121A EA 201890121 A EA201890121 A EA 201890121A EA 201890121 A1 EA201890121 A1 EA 201890121A1
Authority
EA
Eurasian Patent Office
Prior art keywords
chillers
proliferation
natural cell
compositions
interleukin
Prior art date
Application number
EA201890121A
Other languages
Russian (ru)
Other versions
EA038848B1 (en
Inventor
Ён Сок Бэк
Ми Кён Чой
Ю Ра Кан
Original Assignee
Ча Биотек Ко., Лтд.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ча Биотек Ко., Лтд. filed Critical Ча Биотек Ко., Лтд.
Priority claimed from PCT/KR2016/006754 external-priority patent/WO2016209021A1/en
Publication of EA201890121A1 publication Critical patent/EA201890121A1/en
Publication of EA038848B1 publication Critical patent/EA038848B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A61K35/17—Lymphocytes; B-cells; T-cells; Natural killer cells; Interferon-activated or cytokine-activated lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2312—Interleukin-12 (IL-12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2318—Interleukin-18 (IL-18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к способу пролиферации естественных клеток-киллеров и композиции для пролиферации естественных клеток-киллеров, способных улучшать эффект пролиферации клеток и их противораковое действие, где способ включает в себя стадию культивирования естественных клеток-киллеров в культуральной жидкости, содержащей первый интерлейкин (IL-2), второй интерлейкин (по меньшей мере один интерлейкин выбран из группы, состоящей из IL-12, IL-15 и IL-18) и антитело к NKp46.The present invention relates to a method for proliferating natural killer cells and a composition for proliferating natural killer cells capable of improving the cell proliferation effect and their anti-cancer effect, where the method includes the step of culturing natural killer cells in the culture fluid containing the first interleukin (IL- 2), the second interleukin (at least one interleukin is selected from the group consisting of IL-12, IL-15 and IL-18) and anti-NKp46 antibody.

EA201890121A 2015-06-24 2016-06-24 Method for proliferating natural killer cells and compositions for proliferating natural killer cells EA038848B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20150089882 2015-06-24
KR1020160078622A KR101909879B1 (en) 2015-06-24 2016-06-23 Method and Composition for Proliferating of Natural Killer Cell
PCT/KR2016/006754 WO2016209021A1 (en) 2015-06-24 2016-06-24 Method for proliferating natural killer cells and composition for proliferating natural killer cells

Publications (2)

Publication Number Publication Date
EA201890121A1 true EA201890121A1 (en) 2018-06-29
EA038848B1 EA038848B1 (en) 2021-10-28

Family

ID=57797429

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201890121A EA038848B1 (en) 2015-06-24 2016-06-24 Method for proliferating natural killer cells and compositions for proliferating natural killer cells

Country Status (2)

Country Link
KR (1) KR101909879B1 (en)
EA (1) EA038848B1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102265437B1 (en) 2017-11-24 2021-06-15 의료법인 성광의료재단 Composition for culturing NK cell and method for culturing NK cell using the same
KR102311057B1 (en) * 2017-12-14 2021-10-08 (주)녹십자웰빙 Cosmetic composition and pharmaceutical composition for improving atopic dermatis, alopetic, wound, or skin wrinkle
KR102014467B1 (en) * 2017-12-14 2019-08-26 (주)녹십자웰빙 Cosmetic composition and pharmaceutical composition for improving atopic dermatis, alopetic, wound, or skin wrinkle
KR102069704B1 (en) * 2018-05-16 2020-01-23 고려대학교 산학협력단 Method for Expansion of Human NK Cell Using HDAC Inhibitor
KR102081417B1 (en) 2018-11-23 2020-02-25 주식회사 이뮤니스바이오 Use of BTO-1 for proliferating NK cells
JP2022529280A (en) * 2019-04-17 2022-06-20 チャ バイオテック カンパニー リミテッド Natural killer cells with increased anti-cancer activity and their immunotherapeutic uses
CN111944754B (en) * 2020-08-26 2024-04-19 沈阳细胞治疗工程技术研发中心有限公司 Natural killer cell culture method
KR102581230B1 (en) * 2020-10-16 2023-09-21 의료법인 성광의료재단 Natural killer cells with regulated gene expression of anti-cancer effect and uses thereof
CN113832101A (en) * 2021-09-03 2021-12-24 秦红 Preparation method for efficient in-vitro amplification of natural killer cells
KR20230150113A (en) * 2022-04-21 2023-10-30 (주) 엘피스셀테라퓨틱스 Method for producing memory-like nk cell and anticancer use of memory-like nk cell prepared using the same
CN115039764B (en) * 2022-07-14 2024-03-12 北京鼎成肽源生物技术有限公司 Freezing method of natural killer cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010220479A (en) * 2007-06-15 2010-10-07 Medeinetto:Kk Method for culturing nk cell and use of the same
KR101133185B1 (en) * 2008-07-29 2012-04-06 서울대학교병원 Method for Proliferating Natural Killer cell
KR101039843B1 (en) * 2010-08-30 2011-06-09 주식회사 엔케이바이오 A medium composition for cultivating self activated lymphocyte and the cultivation method using the same
KR101298012B1 (en) * 2011-02-08 2013-08-26 (주)차바이오앤디오스텍 Process for preparing lymphocytes comprising activated natural killer cells for targeting cancer cells and pharmaceutical composition comprising the same
CN104204194B (en) * 2011-12-22 2018-08-03 财团法人牧岩生命工学研究所 Produce the method for natural killer cells, by the natural killer cells of this method production and the composition for treating cancer and infectious diseases comprising the natural killer cells

Also Published As

Publication number Publication date
KR20170000798A (en) 2017-01-03
KR101909879B1 (en) 2018-10-19
EA038848B1 (en) 2021-10-28

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