EA202190871A1 - Способы выделения и размножения клеток - Google Patents

Способы выделения и размножения клеток

Info

Publication number
EA202190871A1
EA202190871A1 EA202190871A EA202190871A EA202190871A1 EA 202190871 A1 EA202190871 A1 EA 202190871A1 EA 202190871 A EA202190871 A EA 202190871A EA 202190871 A EA202190871 A EA 202190871A EA 202190871 A1 EA202190871 A1 EA 202190871A1
Authority
EA
Eurasian Patent Office
Prior art keywords
cells
methods
interleukin
reproduction
isolation
Prior art date
Application number
EA202190871A
Other languages
English (en)
Inventor
Шристи Бхандари
Сэмюэл Флоренс
Эндрю Хаттон
Луиза Матиас
Оливер Нуссбаумер
Калле Содерстрём
Марк Уден
Original Assignee
Гаммадельта Терапьютикс Лимитед
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Гаммадельта Терапьютикс Лимитед filed Critical Гаммадельта Терапьютикс Лимитед
Publication of EA202190871A1 publication Critical patent/EA202190871A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • C12N5/0638—Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/24—Gas permeable parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/99—Serum-free medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2302—Interleukin-2 (IL-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2304—Interleukin-4 (IL-4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2309—Interleukin-9 (IL-9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2321—Interleukin-21 (IL-21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Настоящее изобретение относится к способу выделения лимфоцитов (в частности, -Т-клеток) из образца негемопоэтической ткани, включающему этапы культивирования указанного образца негемопоэтической ткани в присутствии (a) интерлейкина-2 (IL-2) или интерлейкина-9 (IL-9), (b) интерлейкина-15 (IL-15) и (c) интерлейкина-21 (IL-21) и сбор популяции лимфоцитов, культивированных из указанного образца негемопоэтической ткани. Предложены способы последующего размножения клеток, а также популяции выделенных клеток, полученные с помощью указанного способа, и способы их применения.
EA202190871A 2018-11-08 2019-11-08 Способы выделения и размножения клеток EA202190871A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18205287 2018-11-08
PCT/GB2019/053164 WO2020095058A1 (en) 2018-11-08 2019-11-08 Methods for isolating and expanding cells

Publications (1)

Publication Number Publication Date
EA202190871A1 true EA202190871A1 (ru) 2021-09-15

Family

ID=64270643

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202190871A EA202190871A1 (ru) 2018-11-08 2019-11-08 Способы выделения и размножения клеток

Country Status (13)

Country Link
US (1) US20220010277A1 (ru)
EP (1) EP3877509A1 (ru)
JP (1) JP2022506836A (ru)
KR (1) KR20210111746A (ru)
CN (1) CN113423820A (ru)
AU (1) AU2019374488A1 (ru)
BR (1) BR112021008461A2 (ru)
CA (1) CA3117895A1 (ru)
EA (1) EA202190871A1 (ru)
IL (1) IL282876A (ru)
MX (1) MX2021005481A (ru)
PH (1) PH12021551037A1 (ru)
WO (1) WO2020095058A1 (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ766453A (en) 2018-02-01 2022-02-25 Nkmax Co Ltd Method of producing natural killer cells and composition for treating cancer
GB202105113D0 (en) 2021-04-09 2021-05-26 Gammadelta Therapeutics Ltd Novel method
EP4381075A1 (en) 2021-08-03 2024-06-12 Gammadelta Therapeutics Ltd Engineering of gamma delta t cells and compositions thereof
WO2023194915A1 (en) 2022-04-04 2023-10-12 Gammadelta Therapeutics Ltd Novel gene armoring
GB202204926D0 (en) 2022-04-04 2022-05-18 Gammadelta Therapeutics Ltd Method for expanding gammadelta T cells

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6028176A (en) 1996-07-19 2000-02-22 Bayer Corporation High-affinity interleukin-4 muteins
CA2349629C (en) * 1998-11-04 2010-01-26 Hemosol Inc. Methods for the production of tcr gamma delta t cells
EP3450538B1 (en) 2003-10-08 2021-08-25 Wilson Wolf Manufacturing Corporation Cell culture methods and devices utilizing gas permeable materials
EP1777294A1 (en) 2005-10-20 2007-04-25 Institut National De La Sante Et De La Recherche Medicale (Inserm) IL-15Ralpha sushi domain as a selective and potent enhancer of IL-15 action through IL-15Rbeta/gamma, and hyperagonist (IL15Ralpha sushi -IL15) fusion proteins
PT1963369E (pt) 2005-11-28 2013-05-28 Zymogenetics Inc Antagonistas de il-21
EP2655409A4 (en) 2010-12-22 2015-07-01 Univ Leland Stanford Junior SUPERAGONISTS AND ANTAGONISTS OF INTERLEUKIN-2
WO2012156958A2 (en) * 2011-05-19 2012-11-22 Instituto De Medicina Molecular Cell line of lymphocytes comprising gamma-delta t cells, composition and production method thereof
RU2739770C2 (ru) * 2014-06-11 2020-12-28 полибайосепт ГмбХ Экспансия лимфоцитов с использованием композиции цитокинов для активной клеточной иммунотерапии
JP2018533373A (ja) * 2015-10-30 2018-11-15 キャンサー・リサーチ・テクノロジー・リミテッド 非造血組織常在性γδ Ｔ細胞の増幅および該細胞の使用
JOP20190224A1 (ar) * 2017-03-29 2019-09-26 Iovance Biotherapeutics Inc عمليات من أجل إنتاج الخلايا اللمفاوية المرتشحة للأورام واستخداماتها في العلاج المناعي
GB201707048D0 (en) 2017-05-03 2017-06-14 King S College London Expansion of gamma delta cells, compositions, and methods of use thereof
WO2018229163A1 (en) * 2017-06-14 2018-12-20 King's College London Methods of activating v delta 2 negative gamma delta t cells

Also Published As

Publication number Publication date
CA3117895A1 (en) 2020-05-14
JP2022506836A (ja) 2022-01-17
AU2019374488A1 (en) 2021-05-27
PH12021551037A1 (en) 2022-01-03
CN113423820A (zh) 2021-09-21
MX2021005481A (es) 2021-11-04
WO2020095058A1 (en) 2020-05-14
EP3877509A1 (en) 2021-09-15
KR20210111746A (ko) 2021-09-13
IL282876A (en) 2021-06-30
US20220010277A1 (en) 2022-01-13
BR112021008461A2 (pt) 2021-08-03

Similar Documents

Publication Publication Date Title
EA202190871A1 (ru) Способы выделения и размножения клеток
EA202190864A1 (ru) Способы выделения и размножения клеток
MX2016016251A (es) Expansion de linfocitos con composicion de citocinas para inmunoterapia celular activa.
AR107278A1 (es) Procedimientos de producción de linfocitos t y linfocitos t producidos por ellos
Wang et al. Differentiation of mesenchymal stem cells from human induced pluripotent stem cells results in downregulation of c-Myc and DNA replication pathways with immunomodulation toward CD4 and CD8 cells
JP2013027385A5 (ru)
WO2018097540A3 (ko) 무혈청면역세포배양용 배지첨가키트, 상기 키트를 이용한 면역세포배양방법, 상기 키트 또는 배양방법에 의해 얻어진 무혈청면역세포배양액 및 상기 배양액을 포함하는 화장료조성물
RU2011121630A (ru) Т-клетки центральной памяти против третьей стороны, способы их получения и их применение в трансплантации и лечении заболеваний
JP2016509840A5 (ru)
MX2018005207A (es) Expansion de celulas t gamma-delta residentes en tejido no hematopoyetico y usos de estas celulas.
EA201891294A1 (ru) Способ, в котором используется биспецифический белковый комплекс
AU2012274478A8 (en) Method for amplifying NK cells
ATE459652T1 (de) Verfahren zur herstellung von antikörpern
TW201612312A (en) Meristematic plant cells and method of isolating them
EA202192714A1 (ru) Система и способ извлечения и криоконсервации костного мозга
ATE544848T1 (de) Cokultur-lte (lymphoid tissue equivalent) für artifizielles immunsystem (ais)
WO2005097979A3 (en) Methods and compositions for obtaining hematopoietic stem cells derived from embryonic stem cells and uses thereof
JP2013537187A5 (ru)
EA201690479A1 (ru) Система и способы непрерывного размножения и поточного получения арбускулярных микоризных грибов в жидкой культуральной среде
EA202091349A1 (ru) Способы культивирования клеток
CN103710307A (zh) Cik细胞培养方法及其应用
Jakob et al. Pluripotent stem cell-derived mesenchymal stem cells show comparable functionality to their autologous origin
ECSP22001155A (es) Protocolo de suspensión de células de cacao campo
JP2016519939A5 (ru)
EP4316497A3 (en) Mesenchymal stem cells as vaccine adjuvants and methods for using the same