EA201791197A1 - Рекомбинантные микроорганизмы, проявляющие повышенный поток при ферментационном пути - Google Patents

Рекомбинантные микроорганизмы, проявляющие повышенный поток при ферментационном пути

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Publication number
EA201791197A1
EA201791197A1 EA201791197A EA201791197A EA201791197A1 EA 201791197 A1 EA201791197 A1 EA 201791197A1 EA 201791197 A EA201791197 A EA 201791197A EA 201791197 A EA201791197 A EA 201791197A EA 201791197 A1 EA201791197 A1 EA 201791197A1
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EA
Eurasian Patent Office
Prior art keywords
energmental
manifesting
way
flow during
increased flow
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EA201791197A
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English (en)
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EA035950B1 (ru
Inventor
Михель Кёпке
Александер Пол Мюллер
Лоан Пхуонг Тран
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Ланцатек Нью Зилэнд Лимитед
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Publication of EA201791197A1 publication Critical patent/EA201791197A1/ru
Publication of EA035950B1 publication Critical patent/EA035950B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/07—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with an iron-sulfur protein as acceptor (1.2.7)
    • C12Y102/07001—Pyruvate synthase (1.2.7.1), i.e. pyruvate ferredoxin oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01005—Acetolactate decarboxylase (4.1.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение относится к рекомбинантной, карбоксидотрофной бактерии Clostridium, которая экспрессирует одну или более пируват:ферредоксин-оксидоредуктазу (ЕС 1.2.7.1), ацетолактатсинтазу (ЕС 2.2.1.6) и ацетолактат декарбоксилазу (ЕС 4.1.1.5). В изобретении дополнительно предложен способ получения продукта ферментации путем ферментации рекомбинантной бактерии в присутствии газообразного субстрата, содержащего СО, для получения одного или более из этанола, бутанола, изопропанола, изобутанола, высших спиртов, бутандиола, 2,3-бутандиола, сукцината, изопреноидов, жирных кислот, биополимеров и их смесей.
EA201791197A 2014-12-08 2015-12-07 Рекомбинантные микроорганизмы, проявляющие повышенный поток при ферментационном пути EA035950B1 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462089053P 2014-12-08 2014-12-08
US201562168969P 2015-06-01 2015-06-01
PCT/US2015/064351 WO2016094334A1 (en) 2014-12-08 2015-12-07 Recombinant microorganisms exhibiting increased flux through a fermentation pathway

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Publication Number Publication Date
EA201791197A1 true EA201791197A1 (ru) 2017-11-30
EA035950B1 EA035950B1 (ru) 2020-09-04

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EA201791197A EA035950B1 (ru) 2014-12-08 2015-12-07 Рекомбинантные микроорганизмы, проявляющие повышенный поток при ферментационном пути

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Country Link
US (1) US10590406B2 (ru)
EP (1) EP3230459B1 (ru)
JP (2) JP6862349B2 (ru)
KR (1) KR102493197B1 (ru)
CN (1) CN107002028A (ru)
AU (1) AU2015360786B2 (ru)
CA (1) CA2969233C (ru)
DK (1) DK3230459T3 (ru)
EA (1) EA035950B1 (ru)
ES (1) ES2836800T3 (ru)
HU (1) HUE052810T2 (ru)
PL (1) PL3230459T3 (ru)
PT (1) PT3230459T (ru)
WO (1) WO2016094334A1 (ru)
ZA (1) ZA201703733B (ru)

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US20160160223A1 (en) 2016-06-09
KR102493197B1 (ko) 2023-01-30
EP3230459A4 (en) 2018-06-20
PL3230459T3 (pl) 2021-03-08
AU2015360786B2 (en) 2021-10-21
EA035950B1 (ru) 2020-09-04
KR20170121147A (ko) 2017-11-01
CA2969233A1 (en) 2016-06-16
JP6862349B2 (ja) 2021-04-21
HUE052810T2 (hu) 2021-05-28
EP3230459A1 (en) 2017-10-18
DK3230459T3 (da) 2020-12-07
ZA201703733B (en) 2020-09-30
PT3230459T (pt) 2020-12-18
CA2969233C (en) 2020-06-23
ES2836800T3 (es) 2021-06-28
JP7139478B2 (ja) 2022-09-20
EP3230459B1 (en) 2020-09-16
JP2021104040A (ja) 2021-07-26
CN107002028A (zh) 2017-08-01
WO2016094334A1 (en) 2016-06-16
US10590406B2 (en) 2020-03-17
AU2015360786A1 (en) 2017-06-15
JP2017536854A (ja) 2017-12-14

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